臺灣博碩士論文加值系統

本實驗以α型與β型幾丁聚醣為原料，分別製成水溶性幾丁聚醣與酸溶性
幾丁聚醣，探討不同去乙醯度、添加量及使用不同的溶劑對種子發芽與水
耕蔬菜抗菌性的影響。實驗分為三個部分：
一、酸溶性幾丁聚醣塗佈於種子對其發芽率的影響。
二、酸溶性幾丁聚醣與水溶性幾丁聚醣對水耕青江菜生長之影響。
三、酸溶性幾丁聚醣對水耕青江菜抗菌性之研究。
所得的實驗結果如下：
將不同去乙醯度、添加量之幾丁聚醣乳酸(0.2M)溶液塗佈於芥藍菜、萵苣
、青江菜、紅莧菜、芹菜、花椰菜的種子上，均能增加其發芽數。去乙醯
度80、90％的幾丁聚醣之結果比70％者好。其中，以去乙醯度80％、1.0
％幾丁聚醣乳酸溶液及去乙醯度90％、1.5%幾丁聚醣乳酸溶液的效果最好
。這可能與種子發芽期間，其內部水分與氧氣達到平衡有關。而做為溶劑
的乳酸、醋酸及幾丁聚醣醋酸溶液均無法增加發芽數。此外也發現，幾丁
聚醣對於種皮較薄的種子，才能增加其發芽數。而對於芽菜類種子，則無
法增加其發芽數。
以水耕栽培法種植青江菜並添加水溶性幾丁聚醣時，先以幾丁聚醣乳酸溶
液塗佈於種子後，再添加120、180ppm的水溶性幾丁聚醣者，其產量較高
。其中，以添加180ppm水溶性幾丁聚醣的效果最好。至於其所含化學成分
的水分、灰分，粗纖維及澱粉含量都無顯著差異。
以酸溶性幾丁聚醣對於青江菜之病原菌Colletotrichum higginsianum 與
Alternaria brassicae Sacc.不具抑菌效果。而對於Cercosporella
brassicae具有良好之抑菌作用。

In this study ,α-chitosan andβ-chitosan were used to prepare
water-soluble and acid-soluble chitosans. Experiments were
designed to investigate the effect of degree of deacetylation
(DD), concentration of chitosans and solvent added to them for
stimulating germination of seeds and for antifungal action to
pathogens on hydroponic vegetable. This research was
constituted of the following three parts：
1.Effects of acid-soluble chitosan on germination of seeds.
2.Effects of acid-soluble and water-soluble chitosans on the
growth of hydroponic Spoon cabbage.
3.Effects of acid-soluble chitosans on antifnngal action to
pathogens of hydroponic Spoon cabbage.
The results obtained were as follows：
The seeds of Chinese kale, lettuce, Spoon cabbage, Amaranthus
ganceticus, celery and cauliflower were coated with different
DD and concentration of chitosans in lactic acid solution
(0.2M), then they were found to be able to stimulate their
germination. The results revealed that chitosans with 80% and
90% DD is better than that of 70% DD. Among them 1.5% chitosan
with 90% DD in lactic acid and 1.0% chitosan with 80% DD in
lactic acid are the best. This may be due to the better balance
of H2O and O2 in seed on
the period of germination. But lactic acid, acetic acid only
and chitosan in acetic acid did not work here. While chitosan
were effective only for the seeds with thinner seed coats, but
not for those with thicker one.
After the seed coated with acid-soluble chitosan, Spoon cabbage
was grown by water-culture and water-soluble chitosan was
added, then the yield is higher for those of adding 120 and 180
ppm water-soluble chitosans, but the latter is the best. As for
their chemical compositions, moisture, ash, crude cellulose and
starch showed no differences.
For the antifungal action of acid-soluble chitosan to Spoon
cabbage, Colletotrichum higginsianum and Alternaria brassicae
were found to none effective, while Cercosporella brassicae was
effective.