臺灣博碩士論文加值系統

本實驗是利用具有催化過氧化氫還原性質的釕金屬氧化物，與葡萄糖
氧化共同修飾在電極上，而發展成葡萄糖生化感測器。並利用電位的選
擇及Nafion膜來避免在測量生化樣品時所遭遇到共存在樣品中的易氧化物
質如ascorbic acid、uric acid等的干擾，以提高此葡萄糖生化感測器的
專一性。此感測器的製備方式是將12.5 %的RuO2混入使用乙酸乙酯稀釋10
倍的碳墨中，直接滴到倒置的石墨旋轉電極上，在室溫下乾燥10分鐘後再
以Nafion膜來固定1.4 units的酵素。此生化感測器偵測葡萄糖的電位
為+0.2 V(vs. Ag/AgCl)，於旋轉電極轉速400 rpm，溫度控制25℃的0.05
M pH 7.4的磷酸緩衝溶液中偵測葡萄糖，電流密度為366.693 nA/mM×cm2
，偵測極限為4.36×10-4 M，反應時間(t10-90 %)為66秒，重複二十次操
作的相對標準偏差為1.894 %。另外，本系統若在4℃下製備乾燥線性可
達50 mM(相關係數為0.99992)。此生化感測器在+0.2 V的電位下偵測，可
完全避開氧氣的催化干擾，並使用Nafion膜可防止生化樣品中具電活性物
質，如ascorbic acid、cystine、uric acid、tyrosine、galactose、
histidine和glutamic acid的干擾。

A glucose biosensor with exceptional linearity was fabricated by
mixing withglucose oxidase and carbon ink. The ruthenium oxides
were prepared with glucoseoxidase was modefied with Nafion
membrane onto the ink surface. Subsequently, the glucose can be
measured at +0.2 V(vs. Ag/AgCl), in 0.05 M pH 7.4
phosphatebuffer solution, 400 rpm, 25 C. The linearly of the
response at best is up to 50 mM (with correlation coefficient
0.99992). The sensitivity of the electrodeis 366 nA/mM.cm2. The
estimated detection limit (S/N=3) is 4.36x10-4 M. The response
time t10%-90% is 66 seconds. The standard deviation of twenties
repetivedetection of 5 mM glucose is 1.894%. There is no
significant interference observedfrom the addition of 0.1 mM
ascorbic acid, 0.4 mM uric acid, 0.2 mM cystine, glutamicacid,
histidine, tyrosine, arabinose, fucose, galatose, lactose,
mannose, raffinose,stachyose, xylose and 10 mM alcohol at
presence or unpresence of 5.5 mM glucose.