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研究生:陳藝婉
研究生(外文):Yi-Wan Chen
論文名稱:鈣離子與TBHP誘發肝細胞起泡機制之探討
論文名稱(外文):Calcium and the plasma membrane blebbing in rat hepatocytes treated with TBHP
指導教授:李宗貴
指導教授(外文):Chong-Kuei Lii
學位類別:碩士
校院名稱:中山醫學院
系所名稱:營養科學研究所
學門:醫藥衛生學門
學類:營養學類
論文種類:學術論文
論文出版年:1999
畢業學年度:87
語文別:中文
論文頁數:93
中文關鍵詞:氧化壓力突起小泡鈣離子維生素EGSH小泡生長
外文關鍵詞:oxidative stressmembrane blebbingcalciumVitamin Eglutathionebleb growth
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當肝細胞受到氧化傷害時,常在細胞膜表面生成許多突起小泡(membrane blebbing)。其生成機制一般認為是相當複雜且是多源性的,包括:細胞膜蛋白質氧化、細胞架構蛋白-肌動蛋白及管蛋白發生改變、胞內鈣離子恆定受到破壞等,其中胞內鈣離子恆定的破壞被認為應是主要因素。
先前實驗已知氧化壓力下維生素E可抑制肝細胞細胞膜起泡的發生;此外,生物體內另一重要抗氧化分子-GSH,也有維持細胞膜蛋白質完整及減少起泡的功能,然而維生素E與GSH抑制起泡的發生是否與胞內鈣離子恆定有關則尚未釐清。因此本實驗將分成二部份來探討鈣離子恆定與起泡的關係:第一部份主要針對胞內鈣離子對於維生素E或GSH抑制肝細胞起泡扮演角色作探討;第二部份則針對胞內鈣離子濃度與細胞起泡過程中小泡生長之關係作探討。
實驗方法:利用初代培養大白鼠肝細胞在未添加或添加100 M維生素E(d--tocopheryl succinate)或 1mM BSO (L-buthionine[S,R]-sulfoximine , 抑制GSH合成)條件下預培養 24小時後,分別加入0.5、1.0、2.0mM t-butyl hydroperoxide (TBHP)誘發氧化壓力,利用倒立式位相差顯微鏡觀察肝細胞起泡程度及小泡大小並以共軛焦顯微鏡觀察胞內鈣離子濃度變化,同時也分析脂質過氧化、胞內硫醇(包括蛋白質硫醇與非蛋白質硫醇)、LDH leakage與肝細胞起泡現象之間的關係。
結果(第一部份):1.當細胞以EGTA (Ethylene glycol-bis[β-aminoethyl ether]-N,N,N’N’-tetraacetic acid)或EGTA+A23187(4-Bromo-calcium ionophore)預處理時,起泡明顯受到抑制,顯示鈣離子對細胞起泡的生成扮演有重要角色;2.TBHP處理下維生素E與GSH均可降低細胞起泡率及LDH leakage,然而維生素E與GSH的保護效果與TBHP濃度有關,在低濃度(0.5mM)TBHP下,GSH對細胞形態具保護效果,然而在高濃度(2mM)TBHP時,則以維生素E保護效果較佳;3.維生素E與GSH的保護作用的確與維持細胞鈣離子的恆定有關;4.維生素E與GSH也可有效抑制脂質過氧化和GSH與蛋白質硫醇的流失,而這可能與其保護細胞形態有關。
在第一部份我們已知鈣離子對細胞起泡的生成扮演有重要角色,在第二部份我們繼續探討在細胞起泡時,鈣離子對小泡直徑生長之影響。結果(第二部份):在2.0mM TBHP誘發氧化壓力時,胞內鈣離子變化與細胞的起泡有密切關係,小泡生成後將快速成長直至破裂,鈣離子濃度的增加在小泡發生前,且小泡長出後鈣離子濃度也呈繼續增加趨勢,但若在小泡形成後不久即加入15mM EGTA 將鈣移除,則不僅可減少細胞起泡率亦可抑制小泡生長速度,同時有部份小泡會縮小最後則消失不見。
綜合以上二個實驗我們認為:1.胞內鈣離子濃度恆定的破壞是TBHP誘發肝細胞起泡因素之一,且鈣離子濃度的變化也會影響小泡的生長;2.維生素E與GSH可維持細胞在氧化壓力下鈣離子的恆定,此作用與維生素E與GSH保護細胞形態有關;3.GSH與維生素E相較下,GSH僅在低濃度TBHP下才具保護效果,但當TBHP濃度增到2.0mM時,則以維生素E保護效果為佳。
目錄
頁次
總摘要……………………………………………………………… 1
前言………………………………………………………………… 3
文獻探討…………………………………………………………… 4
一、 氧化壓力………………………………………………….. 4
二、 生物體內抗氧化系統…………………………………….. 5
(一) 維生素E……………………………………………… 6
(二) 麩胱甘……………………………………………… 6
三、 細胞膜形態變化-起泡……………………………………. 8
(一) 起泡的發生…………………………………………… 8
(二) 起泡的過程…………………………………………… 8
(三) 起泡生成機制………………………………………… 9
A.細胞結構蛋白被破壞…………………………….… 10
B.硫醇的流失與氧化………………………………… 11
C.ATP的耗損………………………………………… 11
D.胞內鈣離子恆定受干擾…………………………… 12
實驗目的……………………………………………………………… 16
材料方法…………………………………………………………… 17
一、 實驗材料…………………………………………………... 17
(一) 實驗動物……………………………………………… 17
(二) 化學試藥……………………………………………… 17
二、 實驗方法………………………………………………….. 19
(一) 肝細胞分離及培養………………………………….. 19
頁 次
(二) 細胞處理及樣品製備……………………………….. 20
(三) 細胞形態變化之觀測…………………………………. 21
(四) 生化分析………………………………………………. 21
A. 細胞存活率測定………………………………….. 21
B. 脂質過氧化測定………………………………….. 22
C. 還原態麩胱甘/氧化態麩胱甘測定…………. 23
D. 細胞膜蛋白質硫醇測定…………………………. 24
(五) 胞內鈣離子濃度分析………………………………… 25
三、 統計分析…………………………………………………… 27
第一部份:胞內鈣離子對於維生素E與GSH抑制肝細胞起泡
扮演角色之探討………………………………………… 28
摘要……………………………………………………………….. 29
結果……………………………………………………………….. 31
一、 氧化壓力下維生素E與麩胱甘(GSH)對肝細胞形
態與存活率之影響……………………………………. 31
二、 維生素E與GSH對肝細胞脂質過氧化之影響…….. 32
三、 鈣離子與細胞膜起泡…………………………………. 33
四、 氧化壓力下,肝細胞鈣離子濃度變化與起泡………. 34
五、 GSH與蛋白質硫醇在TBHP處理下之變化………… 35
結果圖表…………………………………………………………. 37
討論……………………………………………………………… 54
結論(一)……………………………………………………………… 60
第二部份:胞內鈣離子濃度與小泡生長之關係………………….. 61
頁 次
摘要………………………………………………………………. 62
結果與討論………………………………………………………. 63
一、 氧化壓力下,鈣離子對細胞形態與存活率之影響….. 63
二、 鈣離子對細胞小泡生長的影響………………………. 64
三、 鈣移除對小泡生長的影響……………………………. 65
結果圖表…………………………………………………………. 68
結論(二)……………………………………………………………. 78
總結論……………………………………………………………… 79
參考文獻………………………………………………………… 80
附件一: Leibovitz’s L-15 培養基成份…………………………… 92
參考文獻
王振宇(1997),維生素E和麩胱甘保護細胞形態之探討,私立中山醫學院營養科學研究所碩士論文
江明才(1998), 肌動蛋白及管蛋白與肝細胞突起小泡生成關係之探討,私立中山醫學院毒理學研究所碩士論文
Adelman, R.; Saul, R. L.; Ames, B. N. (1988) Oxidative damage to DNA: Relation to species metabolic rate and life span. Proc. Natl. Acad. Sci. USA 85: 2706-2710.
Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, K., and Waston, J. D. (eds.) (1983) in Molecular Biology of the cells. pp549-668. Garland Press, NY.
Ames, B. N.; Shigenaga, M. K.,; Hagen, T. M. (1993) Oxidant, antioxidants, and the degenerative diseases of aging. Proc. Natl. Acad. Sci. USA 90: 7915-7922.
Anderson, M.E. Glutathione and Glutathione Delivery Compounds. (1997) Adv. Pharmacol. 38: 65-78.
Bast, A. and Haenen, G.R. (1990) Regulation of lipid peroxidation by glutathione and lipoic acid: involvement of liver microsomal vitamin E free radical reductase . Adv. Exp. Med. Biol. 264: 111-116.
Bellomo, G., Mirabelli, F. D., DiMonte, D., Richelmi, P., Thor, H. Orrenius, C. and Orrenius, S. (1987) Formation and reduction of glutathione-protein mixrd-disulfides during oxidative stress; a study with isolated hepatocytes and menadione (2-methyl-1,4-naphthoqinone). Biochem. Pharmacol. 36: 1313-1320.
Bennett, J. and Weeds, A. (1986) Calcium and cytoskeleton. Bri. Med. Bull. 42: 385-390.
Berlett , B.S. and Stadtman , E.R. (1997) Protein Oxidation in Aging, Disease, and Oxidative Stress. J. Biol. Chem. 272: 20313-20316.
Berridge, M.J., Bootman, M.D.and Lipp, P. (1998) Calcium-a life and death signal. Nature 395: 645-648.
Berry, M. N. and Friend, D. S.(1973) High yield production of isolated rat liver parenchymal cells: a biochemical and fine structural study. J. Biol. Chem. 59: 722-734.
Bonney, V. R., Becker, J. E., Walker, P. R. and Potfer, V. R.(1974) Primary monolayer cultures of rat parenchymal cells suitable for study regulation of enzymes synthesis. In Vitro 9: 399-413.
Boyne, A. F. and Ellman, G. L. (1972) A methodology for analysis of tissue sulfhydryls components. Anal. Biochem. 46: 639-653.
Brigelius, R., Lenzen, R. and Sies, H. (1982) Increase in hepatic mixed disulfides and glutathione disulfide levels elicited by paraquat. Biochem. Pharmacol. 31: 1637-1641.
Burkart, V., Gro-Eick, A., Bellmann, K., Radons, J. and Kolb, H.(1995) Suppression of nitric oxide toxicity in islet cells by -tocopherol. FEBS Lett. 364: 259-263.
Burnier, M., Centeno, G., Burki, E. and Brunner , H.R. (1994) Confocal microscopy to analyze cytosolic and nuclear calcium in cultured vascular cells. Amer. Physiol.Soc. 193: C1118-C1127.
Burnier, M., G. Centeno, E. Grouzmann, P. Walker, B. Waeber, and H. R. Brunner. (1991) In vitro effects of Dup 753, a nonpeptide angiotensin II receptor antagonist, on human platelets and rat vascular smooth muscle cells. Am. J. Hypertens. 4: 438-443
Cadenas, E. (1989) Biochemistry of oxygen toxicity . Annu. Rev. Biochem. 58: 79-110.
Caprari, P., Bozzi, A., Malorni, W., Bottini, A., Iosi, F., Santini, M.T. and Salvati, A.M. (1995) Junctional sites of erythrocytes skeletal proteins are specific targets of tert-butyl hydroperoxide oxidative damage. Chem-Biol. Interact. 94, 243-258.
Chen, Q. and Stevens. J.L. (1991) Inhibition of Iodoacetamide and t-Butyl hydroperoxide Toxicity in LLC-PK1 Cell by Antioxidants: A Role for Lipid Peroxidation in Alkylation Induced Cytotoxicity. Arch. Biochem. Biophys. 284 , 422-430
Dean, R. T. and Cheeseman, K. H. (1987) Vitamin E protects proteins against free radical damage in lipid environments. Biochem. Biophys. Res. Commun. 148:1277-1282
.
Deressinski, S.C., Ross, W., Sussman, H., Raju, P. A., Anderson, M. T., Moore, W., Ela, S. W. Herzenberg, L. A. and Herzenberg L. A. (1992) Intracellular glutathione levels in T cell subsets in HIV-infected individuals. AIDS Res. Human Retrovirus 8: 305-311
Farber, J. L. (1990) The role of calcium in lethal cell injury. Chem Res Toxicol. 3(6): 503-508
Fawthrop, D. J., Boobis, A. R. and Davis, D. S. (1991) Mechanisms of cell death. Arch. Toxicol. 65:437-444.
Fedier, A. and Keller, H.U. (1997) Suppression of bleb formation, locomotion, and polarity of Walker carcinosarcoma cells by hypertonic media correlates with cell volume reduction but not with changes in the F-actin content. Cell Motil Cytoskeleton, 37(4): 326-337
Ferris, C.D., Huganir, R.L., Bredt, D.S., Cameron, A.M. and Snyder, S.H. (1991) Inositol trisphosphate receptor: Phosphorylation by protein kinase C and calcium calmodulin-dependent protein kinases in reconstituted lipid vesicles. Proc. Natl. Acad. Sci. USA 88, 2232-2235.
Fraga, C. G., Leibovitz, B. E. and Tappel, A. L. (1988) Lipid peroxidation measured as thiobarbituric acid-reactive substances in tissue slices: Characterization and comparison between homogenates and microsomes. Free Radical Biol. Med. 4:155-161.
Friend, J.E. amd Haddad, G.G. (1993) Major differences in Ca2+response to anoxia between neonatal and adult rat CA1 neurons: role of Ca2+ and Na+. J. Neurosci. 13, 63-72
Glascott, P. A., Gilfor, E. and Farber, J. L. (1992) Effect of vitamin E on the killing of cultured hepatocytes by tert-butyl hydroperoxide. Mol. Pharmacol. 41:1155-1162.
Gores, G.J., Herman, B. and Lemasters, J.J. (1990) Plasma Membrane Bleb Formation and Rupture: A Common Feature of Hepatocellular Injury. Hepatology 11: 690-698.
Gudat F, Spichtin HP, Stocklin E, Krey G, Altorfer J, Schmid M Bianchi L. (1983) Electron microscopic studies of delta infection. Prog Clin Biol Res 143:181-189.
Gunter, T. E., and Pfeiffer, D.F. (1990) Mechanisms by which mitochondria transport calcium. Am. J. Physiol. 258: C755-C786
Harman, A.W. and Maxwell, M.J. (1995) An evaluation of the role of calcium in cell injury. Annu. Rev. Pharmacol. Toxicol. 35: 129-44.
Heo, J., Kim, G.H., Lee, K.S., Go, W.U., Ju, H.J., Park, S.K., Song, G.A., Cho, M., Yang, U.S., Moon, H.K., Kim, Y.K. (1997) Effect of Ca2+ channel blockers, external Ca2+ and phospholipase A2 inhibitors on t-butylhydroperoxide-induced lipid peroxidation and toxicity in rat liver slices. Korean J Intern Med , 12 (2): 193-200.
Henschke, P.N., and Elliot, S.J. (1995) Oxidized glutathione decrease luminal Ca2+ content of the endothelial lines (1,4,5)P3-sensitive Ca2+store. Biochem J. 312, 485-489.
Herman, B., Nieminen, A.L., Gores, G.J. and Lemasters ,J.J. (1988) Irreversible injury in anoxic hepatocytes precipitated by an abrupt increase in plasma membrane permeability. FASEB J. 2: 146-151.
Hinshaw, D. B., Burger, J. M., Beals, T. F., Armstrong, B. C. and Hyslop, P. A. (1991) Actin polymerization in cellular oxidant injury. Arch. Biochem. Biophys. 288, 311-316.
Hinshaw, D. B., Sklar, L. A., Bohe, B., Schraufstatter, I., Hyslop, P. A. Rossi, H. W., Spragg, R. G. and Cochrane, C. G. (1986) Am. J. Pathol. 123: 454-464.
Jewell, S.A., Bellomo. G., Thor, H., Orrenius, S. and Smith, M.T. (1982) Bleb formation in hepatocytes during drug metabolism is caused by disturbance in thiol and calcium ion homeostasis. Science 217: 1257-1259.
Jena. B.P., Padfield, P.J., Ingebritsen. T.S. and Jamieson. J.D. (1991) Protein tyrosine phosphatase stimulates Ca2+-dependent amylase secretion from pancreatic acini. J. Biol. Chem. 266: 17744-17746.
Johnson, M.E., Gores, G.J., Uhl. C.B. and Still, J.C. (1994) Cytosolic free calcium and cell death during metabolic inhibition in a neuronal cell line. J. Neurosci. 14: 4040-4049
Jones, D. P. (1995) Glutathione distribution in natural products: absorption and tissue distribution. Methods Enzymol. 252:3-13.
Kagan. V. E. and Quinn, P. J. (1988) The interaction of alpha-tocopherol and homologues with shorter hydrocarbon chains with phospholipid bilayer dispersions. A fluorescence probe study. Eur. J. Biochem,. 171: 661-667.
Kolodney, M. S., and E. L. Elson. (1995) Contraction due to microtubule disruption is associated with increased phosphorylation of myosin regulatory light chain. Proc. Natl. Acad. Sci. USA 92:10252-10256, 1995.
Kosower, N.S. and Kosower, E. M. (1978) The glutathione status of cells. Intl. Rev. Cytol. 54: 109-160.
Kuo, J.H., Chen , H.W., Chou, R.G.R., Lii ,C.K. (1997) Vitamin E protection of cell morphology under oxidative stress is related to cytoskeletal proteins in rat hepatocytes. Arch Toxicol 71:231-237.
Lange, K., and U. Brandt. (1996) Calcium storage and release properties of F-actin: evidence for the involvement of F-actin in cellular calcium signaling. FEBS Lett. 395: 137-142
Lemaster, J. J., DiGuiseppi. J., Nieminen, A-L., and Herman, B. (1987) Blebbing, free Ca2+ and mitochondrial membrane potential preceding cell death in hepatocytes. Nature (London) 325:78-81.
Li, W., Zhao, Y. and Chou, J.-N. (1987) Paraquat-induced cytoskeletal injury in cultured cells. Toxicol. Appl. Pharmacol. 91: 96-106.
Lii, C.-K. and Hendrich, S.(1993) Selenium deficiency supposed the S-glutathionation of carbonic anhydrase III in rat hepatocytes under oxidative stress. J. Nutr. 123: 1480-1486.
Lii, C.-K., Chai, Y.-C., Zhao, W., Thomas, J. A. and Hendrich, S. (1994) S-thiolation and irreversible oxidation of sulfhydryls on carbonic anhydrase III during oxidative stress: a method for studying protein modification in intact cells and tissues. Arch. Biochem. Biophys. 308: 231-239 .
Lii, C.-K. and Hung, C.-N. (1997) Protein thiol modification of human red blood cells treated with t-butyl hydroperoxide. Biochim. Biophys. Acta 29 ; 1336 (2): 147-56.
Liu, H., Miller, E., Water, B., and Stevens, J.L. (1998) Endoplasmic Reticulum Stress Proteins Block Oxidant-induced Ca2+Increases and Cell Death. J. Bio. Chem. 273: 12858-12862
Lkeda, M., Ariyoshi, H., Sakon, M., Kambayash, J.I., Yoshikawa, N., Shinoki, N., Kawasaki, T., Monden, Morito (1998) A role for local calcium gradients upon hypoxic injury in human umbilical vein endothelial cells (HUVEC) Cell Calcium 24(1): 49-57.
Lowry, O. H., Rosebrough , N. J., Farr, A. L. and Randall, R. J. (1951) Protein measurement with folinphenol reagent. J. Biol. Chem. 193: 265-275.
Malorniet, W., Iosi, F., Mirabelli, F. and Bellomo, G. (1991) Cytoskeleton as target in menadione-induced oxidative stress in cultured mammalian cells: alteration underlying surface bleb formation. Chem.-Biol. Interact. 80: 217-236.
Martin, S. J.,O'Brien, G. A., Nishioka, W. K., McGahon, A. J., Mahboubi, A., Saido, T. C. and Green, D. R. (1995) Proteolysis of foldrin (non-erythroid spectrin) during apoptosis. J. Biol. Chem. 270: 6425-6428.
Marcussen, M. (1996) Induction of cell surface blebbing by increased cellular Pi concentration. Biochem. J. 318: 955-958.
Martensson, J., Jain, A., Stole, E., Frayer, W., P., Auld, A.M., Meister, A. (1991) Inhibition of glutathione synthesis in the newborn rat : a model for endogenously produced oxidative stress . Proc. Natl. Acad. Sci. U.S.A. 88: 9360-9364
McConkey, D.J. and Orrenius, S.(1996) The role of calcium in the regulated of apoptosis. J. Leuk. Biol. 59: 775-783.
Mehendale, H. M., Roth, A., Gandolfi, A., J., Klaunig, J. E., Lemasters, J.J., and Cuttis (1994) Novel mechanisms in chemically induced hepatotoxicity. FASEB J. 15: 1285-1295
Meister, A. (1989) Metabolism and function of glutathione. In Glutathione: Chemical, Biochemical, and Medical Aspects (Dolphin, D., Poulson, R. and Avramovic, O., eds) pp367-474, Part A.John Silley & Sons Inc., New York, NY
Mikkelsen, L., Hansen, H. S., Grunnet, N. and Dich, J. (1994) Cytoprotective effect of tocopherols in hepatocytes cultured with polyunsaturated fatty acid. Lipids 29: 369-372.
Mirabelli, F., Salis, A., Marinoni, V., Finardi, G., Bellomo, G., Thor, H. and Orrenius, S. (1988 a) Menadione-induced bleb formation in hepatocytes is associated with the oxidation of thiol groups in actin. Arch. Biochem. Biophys. 264: 261-269.
Mirabelli, F., Salis, A., Perotti, M., Taddei, F., Bellomo, G. and Orenius, S.(1988 b) Alternation of surface morphology caused by the metabolism of menadione in mammalian cells are associated with the oxidation of critical sulfhydryls groups in cytoskeletal proteins. Biochem. Pharmacol. 37:3423-3427.
Miyoshi, H., Umeshita, K., Sakon, M., Shinobu, I.O., Fujitani, K., Gotoh, M., Oiki, E., Kambayashi, J.I. and Monden, M. (1996) Calpain Activation in Plasma Membrane Bleb Formation During tert-Butyl Hydroperoxide-induced Rat Hepatocyte injury. Gastroenterology 110: 1897-1904.
Moldeus, P., Hogberg, J. and Orrenius. S. (1978) Isolation and use of liver cells. Methods Enzymol, 52,55-62.
Murata, A., Monden, M., Umeshita, K., Nakano, H., Kanai, T., Gotoh, M., and Mori, T. (1994) Role of Intracellular Calcium in superoxide-Induced Hepatocyte Injury. Hepatology 19: 1223-1228
Nicotera, P., Bellomo, G., and Orrenius, S. (1990) The role of Ca2+in killing. Chem Res Toxicol. 3(6): 484-494
Nicotera P., Thor H., Orrenius S. (1989) Cytosolic-free Ca2+ and cell killing in hepatoma 1c1c7 cells exposed to chemical anoxia. FASEB J. 3:59-64
Nieminen A.L., Gores G.J., Dawson T.L., Herman B, Lemasters J.J. (1990) Toxic injury form mercuric chloride in hepatocytes. J Biol Chem 265: 2399-2408
Nieminen, A. L., Gores G.J., Wray, B.E., Tanaka, Y., Herman, B. and Lemasters, J.J.(1988) Calcium dependence of bleb formation and cell death in hepatocytes. Cell Calcium 9: 237-246.
NiKi, E. (1991) Action of ascorbic acid as a scavenger of active and stable oxygen radicals. Am. J. Clin. Nutr. 54: 1119S-1127S.
Orrenius, S., McConKey, D.P., Jones , D. J. and Nicotera , P (1988) Ca2+-activated mechanisms in toxicity and programmed cell death. ISI Atlas Sci. Pharmacol. 2: 319-324.
Palmanda, J.R. and Kehrer, J.P.(1993) Involvement of vitamin E and protein thiol in the inhibition of microsomal lipid peroxidation by glutathione. Lipid 28, 427-431
Perntki, M., Chaponnier, M, Jeanreneaud, B. and Gabbinai, G. (1979) Actin microfilaments, cell shape, and secretory processes in isolated rat hepatocytes. J. Cell Biol. 81: 592-607.
Phillips MJ, Powell S, Patterson J, Valencia P , eds. (1987) The liver: an atlas and text of ultrastructural pathology. New York: Raven Press,46, 72,87,107,146,351,522-523.
Potter, D. W. (1980) High-performance, glutathione liquid chromatography analysis of anaomole levels of glutathione, glutathional disulfide, and related thiols and disulfides. Ana Biochem. 106:55-62.
Ray, S.D. and Fariss, M.W. (1994) Role of cellular energy status in tocopheryl hemisuccinate cytoprotection against ethyl methanesulfonate-induced toxicity. Arch. Biochem. Biophys. 311 :180-190..
Reed, D. J., Pascoe, G. A. and Olafsdotter, K. (1987) Some aspects of cell defense mechanisms of glutathione and vitamin E during cell injury. Arch. Toxicol. 11(suppl.) 34-38.
Reed, D. J., Babson, J. R., Beatty, P. W., Brodie, A. E., Ellis, W. W. and Potter, D.W. (1980) High —performance liquid chromatography analysis of nanomole levels of glutathione, glutathional disulfide, and related thiols and disulfides. Ana. Biochem. 106: 55-62.
Rokutan, K., R. B. Jr. and Kawai, K. (1994) Oxidative stress induces S-thiolation of specific proteins in cultured gastric mucosal cells. Am. J. Physiol. 266: G247-G254.
Rosania, G. R., and Swanson. J.A. (1996) Microtubules can modulate pseudopod activity from a distance inside macrophages. Cell. Motil. Cytoskeleton 34: 230-245.
Rush, G.F., Gorski, J. R., Ripple, M.G., Sowinski, J., Bubelski, P. and Hewitt, W.R. (1985) Organic hydroperoxide-induced lipid peroxidation and cell death in isolated hepatocytes. Toxicol. Appl. Pharmacol. 78: 473-483
Sakaida, I., Thomas, A.P., Farber, J.L. (1991) Increase in Cytosolic calcium ion concentration can be dissociated form the killing of cultured hepatocytes by tert-butyl hydroperoxide. J. Biol. Chem. 266: 717-722.
Sano M., Motchnik P.A., Tappel A.L. (1986) Halogenated hydrocarbon and hydroperoxide-induced peroxidation in rat tissue slices. J Free Radic Biol Med
Schuessler, H., and Schilling, K. (1984) Oxygen effect in the radiolysis of proteins Part 2. Bovine albumin. Int. J. Radiat. Biol. Relat. Stud. Chem. Med, 45(3): 267-281.
Smith, M. T., Evans, C. G., Thor, H. and Orrenius, S. (1985) Quinone-induced oxidative injury to cells and tissues. in Oxidative Stress(Sies, H., ed.), pp91-113. Academic Press, New York, NY.
Stournaras, C., Drewes, G., Blackholm, H., Merkler, I. and Faulstich, H (1990) Glutathionyl(cysteine-374) actin forms filaments of low mechanical stability. Biochim. Biophys. Acta 1037: 86-91.
Stall, F. J. T., Roederer, M., Herzenberg, L. A. and Herzenberg L. A. (1991) Intracellular thiols regulate activation of nuclear kB and transcription of human immunodeficiency virus. Proc. Natl. Acad. Sci. USA 87: 9943-9947
Stall. F.J. T., Roederer, M., Israeiski, D. M. Bubp, J., Mole, L. A., McShane, D., Deressinski, S.C., Ross, W., Sussman, H., Raju, P. A., Anderson, M. T., Moore, W., Ela, S. W. Herzenberg, L. A. and Herzenberg L. A. (1992) Intracellular glutathione levels in T cell subsets in HIV-infected individuals. AIDS Res. Human Retrovirus 8: 305-311.
Takenaka, Y., Miki, M., Yasuda, H. and Mino, M, (1991) The effect of -tocopherol as an antioxidant on the oxidation of membrane protein thiols induced by free radicals generated in different sites. Arch. Biochem. Biophys. 285: 344-350.
Thor, H., Smith, M. T., Hartzell, P., Bellomo, G., Jewell, S.A. and Orrienius, S. (1982) The metabolism of menadione (2-methyl-1,4-naphthoquinone) by isolated hepatocytes. J. Biol. Chem. 257: 12419-12425.
Torgerson, R.R. and McNiven, M.A. (1998) The actin-myosin cytoskeleton mediates reversible agonist-induced membrane blebbing. J. Cell Sci. 111, 2911-2922
Trump, B. F., Berezesky, I. And Phelps, P.C. (1981) Sodium and calcium regulation and the role of the cytoskeleton in the pathogenesis of disease: a review and hypthesis. in Scanning Electron Microscopy (O'Hara, A. M. F., ed.), pp435-454. SEM Inc., Chicago, IL.
Tsai, W.J., Chen, J.C., Wang, C.T. (1988) Changes in both calcium pool size and morphology of human platelets incubated in various concentration of calcium ion. Calcium-specific bleb formation on platelet-membrane surface. Biochin Biophys Acta 9; 940(1): 105-120 .
Urano, S., Inomori, Y., Sugawara, T., Kato, Y., Kitahara, M., Hasegawa, Y., Matsuo, M. and Mukai, K. (1992) Vitamin E: inhibition of retinol-induced hemolysis and membrane-stabilizing behavior. J. Biol. Chem. 267: 18365-18370.
Van Haelst, C., and Rothstein, T.L. (1988) Cytochalasin stimulates phosphoinositide metabolism in murine B lymphocytes. J. Immunol. 140: 1256-1258.
Van Winkle. W. B., Snuggs. M., Miller. J. C. and Buja. L. M. (1994) Cytoskoletal alterations in cardiomyocytes following exposure to the lipid peroxidation product, 4-hydroxynonenal. Cell Motil. Cytoskel. 28: 119-134.
Virtanen, I. and Miettinen, A. (1980) The role of actin in the surface integrity of cultured rat liver a cells. Cell Biol Int Rep 4: 29-36.
Wang, S.-T., Kuo, J.-H, Chou, R.-C. R. and Lii, C.-K. (1996) Vitamin E protection of cell morphology and protein thiols in rat hepatocytes treated with tert-butyl hydroperoxide. Toxicol. Let. 89: 91-98.
Weeds A. (1982) Actin —binding proteins: regulators of cell archltecture and motility. Nature 296: 811-816.
White, C.A., Thannickal, V.J. , Fanburg, B.L. (1994) Glutathione deficiency in human disease. J. Nutr. Biochem. 5: 218-226.
Wyllie, A.H. (1980) Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature 284: 555-556 .
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