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研究生:王佩瑾
研究生(外文):Pei-chin Wang
論文名稱:香菇太空包堆肥抑制胡瓜猝倒病菌的特性
論文名稱(外文):Characteristics for Inhibition of Cucumber Damping-off Pathogen by Spent Forest Mushroom Compost
指導教授:黃振文黃振文引用關係
指導教授(外文):Jenn-Wen Huang
學位類別:碩士
校院名稱:國立中興大學
系所名稱:植物病理學系
學門:農業科學學門
學類:植物保護學類
論文種類:學術論文
論文出版年:1999
畢業學年度:87
語文別:中文
論文頁數:61
中文關鍵詞:胡瓜猝倒病菌農業廢棄物香菇太空包堆肥栽培介質抑病介質水溶性浸出液
外文關鍵詞:Agriculture wastescucumber damping-offculture mediumspent forest mushroom compostsuppressive mediumwater-soluble filtrate
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利用四種香菇生長基質資材與香菇太空包不同腐熟程度之堆肥栽培胡瓜幼苗,發現完熟的香菇太空包堆肥(Spent forest mushroom compost;SFMC)最適於胡瓜幼苗的生長,故本試驗選SFMC作為栽培胡瓜幼苗的介質。將胡瓜猝倒病菌Pythium aphanidermatum Pa104和Pa105菌株以四種方法接種於SFMC與荷蘭泥炭苔(Bas Van Burren No.4 peat moss;BVB )兩介質中,結果發現游走子接種法可以穩定測出SFMC抑制胡瓜猝倒病發生的效果比BVB優異。將SFMC與BVB按不同比例混合後,接種P. aphanidermatum Pa104 和 Pa105菌株,結果發現介質中含有SFMC比例愈高,胡瓜發生猝倒病的百分率愈少。將本菌Pa104 和 Pa105兩菌株接種在SFMC中,14天後,菌體的存活率降至10%以下,直到第28天時,便無法測得P. aphanidermatum 兩菌株的存活,證明本菌不易在SFMC中存活 。熱蒸汽(100℃,20min)與高溫高壓(121℃,15lb,20min)處理無法有效破壞SFMC抑制胡瓜猝倒病的功效。探討SFMC與BVB兩介質之水溶性浸出液對本菌兩菌株產生游走孢子囊、游走孢子、藏精器、及藏卵器的影響,結果發現SFMC水溶性浸出液較BVB浸出液更具有顯著抑制本菌產生游走子、藏精器及藏卵器的效果;然而,兩介質的水溶性浸出液對本菌產生游走孢子囊的數量間卻無顯著差異。本菌兩菌株游走子的釋放量隨SFMC之電導度(EC)下降而增加。在SFMC與BVB的EC值相近時,兩菌株的游走子釋放量亦隨之趨於相近。pH值不會影響兩介質浸出液的電導度;惟pH值介於7至9間,SFMC浸出液對本菌兩菌株之發芽管的抑制效果最為顯著。高溫高壓處理兩介質後抽取水溶性浸出液或由介質抽取浸出液經高溫高壓處理,皆可部份紓解兩者水溶性浸出液抑制病原菌發芽管的生長,惟介質先經熱處理再抽取浸出液者,其抑制病原菌靜止子之發芽管生長的效果較為顯著。本菌在熱處理過之SFMC中,亦比在BVB介質中不易存活。利用SFMC培育之胡瓜根、莖及子葉較BVB所培育者更具有抵抗P. aphanidermatum纏據的效果;惟兩者所培植之胡瓜根系經蒸餾水漂洗後的抗菌效果,即會隨之下降,致使本菌纏據植體的比率因而提高。SFMC所培育之植株的根與莖比BVB所培育者含有較高量的過氧化酵素活性,惟由SFMC所培育之胡瓜子葉卻比BVB培植者含有較低的過氧化酵素活性。此外,由SFMC培育之胡瓜子葉含有K、Ca、Mg、NO2--N和NO3--N的量均顯著較BVB所培植者高。綜合上述研究成果證明香菇太空包堆肥抑制胡瓜猝倒病菌的原理主要歸因於非生物因子扮演著重要的角色。

Of the four agriculture wastes tested, spent forest mushroom compost (SFMC) was more suitable as substrates for growth of cucumber seedlings than raw growth medium for cultivation of forest mushroom (RGM), raw spent forest mushroom growth medium (RFM), and raw spent forest mushroom growth medium contaminated with other microorganisms [ RFM (C) ]. Inoculation method of zoospore suspension provided a stable and accurate means of assessing the effect of SFMC and Bas Van Burren No.4 peat moss (BVB) on damping-off incidence of cucumber seedlings. SFMC was more effective in suppressing damping-off of cucumber seedlings caused by Pythium aphanidermatum isolate Pa104 and Pa105 as compared with BVB. Five SFMC-BVB medium combinations were prepared by mixing SFMC with BVB at rates of 4:0, 3:1, 2:2, 1:3, and 0:4 (v/v) and inoculated with zoospore suspension of the pathogen. The more concentration of SFMC the medium was amended, the less incidence of damping-off the cucumber seedlings were grown in the medium for 12days. SFMC was not suitable for survival of P. aphanidermatum isolates Pa104 and Pa105 because the fungi could not be detected by Corn meal-pimaricin-rose bengal-streptomycin-benomyl agar 28 days after their perlite inoculum were added into SFMC medium. The suppressive effect of SFMC medium on infection of cucumber seedlings by P. aphanidermatum isolates Pa104 & Pa105 was not reduced after it was steamed at 100℃ hot air for 20 min. or autoclaved at 121℃, 15lb, for 20 min. The water-soluble filtrate extracted from SFMC could markedly inhibit formation of oOgonia and antheridia of P. aphanidermatum isolates Pa104 & Pa105 and reduce production of zoospores as compared with BVB's water-soluble filtrate. However, filtrates from SFMC and BVB were not significantly different in affecting the formation of zoosporangia. The electrical conductivity values of SFMC filtrate were highly correlated with production of zoospores by the pathogen. The inhibitory effect of filtrate from SFMC medium were partially nullified after it was steamed and autoclaved. However, the steamed and autoclaved SFMC medium still showed adverse effect on survival of the pathogen. Cucumber seedlings grown in SFMC medium were more effective in inhibiting colonization of root, stem, and cotylendon by the pathogen than ones in BVB medium. The peroxidase activity, nutrient elements such as K, Ca, Mg, NO3--N and NH4+-N in root and stem from cucumber seedlings grown in SFMC medium were significantly higher than those in BVB medium. The results suggest that abiotic factors may play an important role on reduction of damping-off incidence of cucumber seedlings in SFMC medium.

前言………………………………………………………………………1
材料與方法………………………………………………………………5
供試植株……………………………………………………………5
病原菌分離及病原性測定…………………………………………5
病原菌鑑定…………………………………………………………6
接種源及靜止子(encysted zoospores)的製備……………………6
供試堆肥介質………………………………………………………8
堆肥(介質)水溶性浸出液的製作………………………………8
堆肥介質之化學成分分析…………………………………………8
香菇生長基質資材與香菇太空包堆肥對胡瓜生長的影響………10
介質對胡瓜猝倒病發生的影響……………………………………10
不同接種方法探討SFMC抑制胡瓜猝倒病的效果………10
病原菌在SFMC與BVB介質中的存活……………………………10
高溫高壓對SFMC介質抑制胡瓜猝倒病發生的影響……………11
介質水溶性浸出液對Pythium aphanidermatum 產生游走子的影響……………………………………………………………………11
SFMC與BVB水溶性浸出液的電導度對P. aphanidermatum 產生游走子的影響………………………………………………………12
SFMC及BVB 浸出液對P. aphanidermatum產生游走孢子囊、游走孢子、藏精器及藏卵器的影響…………………………………12
不同酸鹼值之SFMC及BVB水溶性浸出液對P. aphanidermatum靜止子發芽率及發芽管生長的影響………………………………12
熱蒸氣或高溫高壓對SFMC及BVB 水溶性浸出液抑制Pythium aphanidermatum 靜止子發芽管的影響……………………………13
高溫高壓處理過之介質對胡瓜猝倒病菌存活的影響……………13
由SFMC與BVB介質所培育之胡瓜植體抗拒Pythium aphanidermatum纏據的效果………………………………………14
胡瓜植體之過氧化酵素活性分析…………………………………15
胡瓜植體化學分析…………………………………………………15
結果……………………………………………………………………17
病原菌鑑定…………………………………………………………17
菌株之致病毒力比較………………………………………………17
香菇太空包生長基質與香菇太空包堆肥對胡瓜生長的影響……17
評估SFMC對胡瓜猝倒病發生的影響……………………………18
不同接種方法探討SFMC抑制胡瓜猝倒病發生的效果…18
SFMC與BVB對Pythium aphanidermatum存活的影響…………19
高溫高壓對SFMC與BVB介質抑制胡瓜猝倒病發生的影響……19
SFMC與BVB介質之化學成分分析………………………………19
SFMC與BVB水溶性浸出液對Pythium aphanidermatum 產生游走孢子囊、游走孢子、藏精器及藏卵器的影響
………………………………………………………………20
熱蒸氣與高溫高壓對SFMC與BVB浸出液抑制Pythium aphanidermatum發芽管的影響…………………………21
胡瓜猝倒病菌在高溫高壓處理過之介質中的存活………………21
SFMC與BVB兩介質培育胡瓜植體抗拒Pythium aphanidermatum纏據的效果………………………………………21
胡瓜植株根、莖及子葉之過氧化酵素活性分析…………………22
植體化學成分分析測定……………………………………………22
討論……………………………………………………………………23
參考文獻………………………………………………………………27
中文摘要………………………………………………………………33
英文摘要………………………………………………………………35
圖表……………………………………………………………………37
附錄……………………………………………………………………61

王才義。1993。園藝作物生產與發展研討會專刊。69-82頁。國立中興大學園藝系編印。台中。
杜自疆。1987。食用菇栽培技術(第十一版)。豐年社編印。台北。
林景和。1993。太空包菇類廢渣之農業利用。永續農業研討會專集。p.93-102。台中區農業改良場編印。彰化。
吳文希。1996。健康作物幼苗培育的溫床-栽培介質的調製。健康清潔植物培育研討會專刊 p.113-115。 中華民國植病學會出版。
邱安隆。1996。利用農工廢棄物研製可供培育蔬菜種苗之抑病介質。國立中興大學植物病理研究所碩士論文。
邱安隆、黃振文。1997。農工廢棄物堆肥培育蔬菜幼苗與抑制菜苗根部病害的效果。植病會刊 6:67-72。
郭魁士。1990。土壤學(第六版)。之宜出版社。台北。
張愛華。1981。本省現行土壤測定方法。作物需肥診斷技術。台灣省農業試驗所印。台中。
黃振文。1993。開發有機添加劑防治作物病害的系列研究。永續農業研討會專集 p.227-237。台中區農業改良場編印。彰化。
黃錦河。1995。本土化蔬菜穴盤育苗介質之開發利用。國立中興大學園藝研究所碩士論文。
Assche, C. van., and Uyttebroeck, P. 1981. The influence of domestic waste compost on plant diseases. Acta Hort. 126:169-178.
Bateman, D. F. 1964. An induced mechanism of tissue resistance to polygalacturonase in Rhizoctonia-infected hypocotyls of bean. Phytopathology 54: 439-445.
Boehm, M. J., Madden, L. V., and Hoitink, H. A. J. 1993. Effect of organic matter decomposition level on bacterial species diversity and composition in relationship to Pythium damping-off severity. Appl. Environ. Microbiol. 59:4147-4149.
Bremner, J. M. and Mulvaney .1982. Nitrogen-total. Pages 595-624, in Methods of Soil Analysis Part 2. 2nd ed . Page, A. L. et al (eds ), Am. Soc. of Agron Inc. Madison,Wis.
Cataldo, D. A., Haroon, M., Schader, L. E., and Youndgs, V. L. 1975. Determination of itrate in plant tissues. Soil Sci. Plant Nutri. 6(1):71-80.
Chen, W., Hoitink, H. A. J., and Madden, L. V. 1988. Microbial activity and biomass in container media for predicting suppressiveness to damping-off caused by Pythium ultimum. Phytopathology 78: 1447-1450.
Cruickshank, I. M., and Perrin, D. R. 1964. "Pathological function of phenolic compounds in plants" Biochemistry of Phenolic Compounds. Academic Press. London .
Daft, G. C., Poole, H. A., and Hoitink, H. A. J. 1979. Composted hardwood bark: A substitute for steam sterilization and fungicide drenches for control of poinsettia crown and root rot. Hortscience 14: 185-187.
De Boodt, M., and Verdonk, O. 1972. The physical properties of the substances in horticulture. Acta Hort. 26: 37-42.
Doll, E. C., and Lucas, R. E. 1973. Testing soil for potassium, calcium and magnesium. Pages 133-152 in Soil Testing and Plant Analysis .Walsh , L. M. and Beaton, J. D. (eds.), Soil Sci. Soc. of Am. Madison, Wis.
Harada, Y., Inbar., and Chew, Y. 1985. Effect of compost maturity on tomato seedling growth. Sci. Hortic. 27:199-208.
Hardy, G. E. S. J., and Sivasithamparam, K. 1991a. Suppression of Phytophthora root rot by a composted Eucalyptus bark mix . Aust. J. Bot. 39:153-159.
Hardy, G. E., S. J., and Sivasithamparam, K. 1991b. Effects of sterile and non-sterile leachates extracted from composted eucalyptus bark and pine-bark container media on Phytopathora spp. Soil Biol. Biochem. 23:25-30.
Hoitink, H. A. J., and Pool, H. A. 1980. Factor affecting quality of composts for utilization in container media. Hortscience 15:13-20.
Hoitink, H. A .J., and Fahy, C. P. 1986. Basis for the control of soilborne plant pathogens with composts. Annu. Rev. Phytopathol. 24:93-114.
Kao, Ching-Wen 1985. The role of calcium and microorganisms in suppression of Pythium splendens in a pasture soil from South Kohala, Hawaii. Ph. D. dissertation, the University of Hawaii. pp.22-27.
Kato, K., Fukaya, M., and Tomita, I. 1981. Effect of successive applications of various soil amendments on tomato fusarium wilt. Res. Bull. Aichi-Ken Agric. Res. Cent. 13:199-208.
Kirchmann, H., and Witter, E. 1989. Ammonia Violatilization during aerobic and anaerobic manure decomposition. Plant and Soil 115:35-41.
Kuc, J. P., and Preisig, C. 1984. Fungal regulation of disease resistance mechanisms in plants. Mycologia 76: 767-784.
Kuwatsuka, S., and Shindo, H. 1973. Behavior of phenolic substance in the decaying process of plants.I. Identification and quatitative determination of phenolic acids in rice straw and its decayed product by gas chromatography. Soil Sci.Plant Nutri. 19:219-227.
Lin, Y. S., Yang, C. H., and Stanghellini, M. E. 1992. The relationships between inoculum density and absolute inoculum potential of Pythium aphanidermatum. Plant Pathol. Bull. 1:13-18.
Lee, Y. S., and Bartlett, R. J. 1976. Stimulation of plant growth by humic 0substances. Soil Sci. Soc. Am. J. 40:876-879.
Lo, C.T., and Lin, Y. S. 1992. Inhibition of Pythium aphanidermatum by volatile substances from soil amended with S-H mixture. Plant Pathol. Bull. 1:206-209.
Loehr, R.C. 1974. Agriculture Waste Management .Academic press. London.
Loehr, V.I., Wang, S. H., and Wolt, J. D. 1984. Physical and chemical characteristic of fresh and aged spent mushroom compost. Hortscience 19:681-683.
Malek, R. B., and Gartner, J. B. 1975. Hardwood bark as a soil amendment for suppression of plant parasitic nematodes on container-growth plant. Hortscience 10: 33-35.
Mandelbaum, R., and Hadar, Y. 1990. Effects of available carbon source on microbial activity and suppression of Pythium aphanidermatum in compost and peat container medium. Phytopathology 80:794-804.
Nelson, P.V. 1991. Greenhouse Operation and Management. 3rd ed. Reston Pub. Co. Inc. Virginia, U.S.A.
Pacumbaba, R. P., Beyl, C. A., and Pacumbaba, R. O., Jr. 1999. Shiitake mycelial leachate suppresses growth of some bacterial species and symptoms of bacterial wilt of tomato and lima bean in vitro. Plant Dis. 83: 20-23.
Parr, J. F., and Willson, G. B. 1980. Recycling organic wastes to improve soil productivity. Hortscience 15:162-166.
Sachindra, N. M., Kageyama, K., and Hyakumachi, M. 1996. Decreased germinability and virulence of oospores of Pythium aphanidermatum in relation to loss of endogenous carbon during incubation on soil. Soil Biol. Biochem. 28: 545-553.
Schmilenski, G. K. 1991. Quality control and use of composted organic wastes as components of growing media in the federal republic of Germany. Acta Hort. 294:89-181.
Stanghellimi, M.E., and Russell, J. D. 1973. Germination in vitro of Pythium aphanidermatum oospores. Phytopathology 63:133-137.
Stills, S.M., Dirr, M.A., and Gartner, J.B. 1976. Phytotoxic effects of several bark extracts on mung bean and cucumber growth. J. Amer. Soc. Hort. Sci. 101: 34-37.
Tamura, M., and Taketami, K. 1977. Biology and control culbroot of Chinese cabbage in the ishikawa prefecture. Ishikawa Pref. Agric. Exp. Stn. Bull. 9: 1-26.
Van Der Plaats-Niterink, A. J. 1981. Monograph of the Genus Pythium. Studies in Mycology No.21, Centraalbureau Voor Schinnelcultures, Baarn, the Netherlands. 242pp.
Worall, R.J. 1981. Comparison of composted hardwood and peat-based media for the production of seedlings, foliage and flowering plants. Sci. Hor .15:311-319.
Zhang, W., Dick, W. A., and Hoitink, H. A. J. 1996. Compost-induced systemic acquired resistance in cucumber to Pythium root rot and anthracnose. Phytopathology 86:1066-1070.

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