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研究生:王靜儀
論文名稱:以倒立式顯微鏡探究血小板於不同合成PU上之吸附情形
論文名稱(外文):Platelet Adhesion Study of the Synthesized Polyurethanes by Inverted Optical Microscope with Image System
指導教授:林睿哲
學位類別:碩士
校院名稱:國立成功大學
系所名稱:化學工程學系
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:1999
畢業學年度:87
語文別:中文
論文頁數:43
中文關鍵詞:血小板吸附血小板活化光學顯微鏡
外文關鍵詞:platelet adhesionmorphologyoptical microscope
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體外血液相容性測試非常依賴影像系統提供足夠多量之資訊,以作進一步之分析及血液相容性判斷。本實驗主要是建立以倒立式光學顯微鏡為主之影像系統,觀察血小板吸附實驗之材質表面,輔以數位式影像擷取器做數位影像擷取,所取得之影像再經電腦軟體處理、分析。本系統之優點在於1.)可取代SEM為血小板吸附實驗觀測儀器,避免SEM試片前處理對表面所造成之不確定干擾,且操作費用便宜;2.)可應用於其他表面微觀觀測;3.) 連續監測系統之建立有助發展體外(in vivo)流動態(in situ)實驗,有助研究血小板吸附之動力機構,本系統可進一步應用於流動態血液吸附實驗觀測。
本實驗中,共選擇了10種材料表面於顯微鏡下觀察血液吸附測試結果,其中包含了8種PU並參考以往文獻以酸氧化處理玻璃表面作為對照組。血液相容性測試結果,PU中以含氟之PU血小板吸附量較少,而含離子基COONa及含SO3Na等離子性PU則表現較差。在實驗過程中,發現不同於以往酸氧化處理的玻璃表面血小板瘋狂吸附之情形,經NaOH浸泡處理過之玻璃,在血液相容性上有優異之表現,此情形非常特別。

Platelet adhesion is one of the ways for hemocompatibility assessment, in which great amount of image information is in demand for in vitro investigation. The aim of this study is to set up an image system for the observation for the phenomena occurring during blood-material contact. With the aid of CCD, frame grabber and computer etc., images of the material surface before and after the platelet contact were obtained for further analysis of platelet adhesion density and adherent platelet morphology。
There were 10 materials tested, including eight kinds of polyurethanes (PU) and two kinds of treated glasses. The fluorine-containing PUs showed lower platelets adhesion density while the ion-containing PUs (COONa and SO3Na-containing polyurethanes) showed higher values. Amazingly, NaOH treated glass, unlike well-studied acid-oxidized-glass, was found to be relatively better in platelet compatibility, in terms of lower platelet adhesion density and less activated platelet morphology.
The system was examined to function well from static platelet adhesion experiments. The advantages of this image system comparing with SEM are cheaper in operating and is facilitating to hemocompatibility study in flow system。

摘要I
AbstractII
目錄III
表目錄III
圖目錄III
目 錄
壹、緒論…………………………………………..….1
貳、文獻回顧………………………………………….4
2-1 血液相容性之探討…………………………………4
2-1.1 血液之成分…………………………………..........4
2-1.2 血小板之機能(Platelet Functions)………………....5
2-1.3 凝血作用…………………………………..............8
2-2 聚胺基甲酸乙酯(Polyurethane,PU)簡介…………10
2-3 實驗分析設備…………………………………....12
參、研究方法 14
3-1 PU溶液 14
3-1.1 實驗室合成PUs 14
3-1.2 商業PU 14
3-2 蓋玻片[Cover glass]的前處理17
3-2.1 NaOH處理之玻璃 17
3-2.2 Acid處理之玻璃 17
3-2.3 PU鍍膜用之玻璃 18
3-3 PU成膜 18
3-4 血小板吸附性質之測試 19
3-5影像擷取系統 21
肆、實驗結果 23
4-1 接觸角 23
4-1.1 靜態接觸角(Static Contact Angle)測試結果23
4-1.2 動態接觸角(Dynamic Contact Angle) 24
4-2化學分析電子光譜儀(Electron Spectroscopy for Chemical Analysis, ESCA)量測結果25
4-3血小板吸附實驗結果27
4-3.1 血小板吸附密度27
4-3.2 血小板活化情形.34
伍、討論與建議39
5-1影像系統建立32
5-2影像分析33
5-3玻片表面40
5-4 PU表面 41
參考文獻

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