跳到主要內容

臺灣博碩士論文加值系統

(44.211.34.178) 您好!臺灣時間:2024/11/03 09:08
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:張正宏
論文名稱:離體培養及新鮮分離之血管內皮細胞的電生理特性
論文名稱(外文):Electrophysiological Characterization of Cultured and Freshly Isolated Vascular Endothelial Cells
指導教授:任卓穎
指導教授(外文):Jen. Chauying J
學位類別:碩士
校院名稱:國立成功大學
系所名稱:生理學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:1999
畢業學年度:87
語文別:中文
中文關鍵詞:內皮細胞電生理細胞培養
外文關鍵詞:endothelial cellspatch-clampcell culture
相關次數:
  • 被引用被引用:0
  • 點閱點閱:223
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
內皮細胞為血管組織與血液之理想介面,控制著物質的通透及調控血管張力,並調節血小板及白血球的活性,在生理上有著多功能的角色。內皮細胞常被視為非興奮性細胞,其運作多與細胞內訊息傳導有關。近年來內皮細胞之離子通道功能與角色漸漸被瞭解,細胞藉由細胞膜對離子通透性的改變與細胞膜電位的改變使細胞活化,從文獻中指出若阻斷細胞膜上氯離子或鉀離子通道,則影響鈣離子流入細胞,與鈣離子相關之訊息傳導途徑將受阻﹔此外文獻中指出離體培養之內皮細胞比正常體內之細胞對一些血管活化因子之刺激較為遲鈍。所以我們的實驗以人類臍帶靜脈內皮細胞(HUVEC)為對象,分別以酵素新鮮分離(enzymatic freshly isolated)、機械力新鮮分離(mechanical freshly isolated)及酵素分離培養第一代之細胞(cultured first passage),以傳統全細胞記錄法(conventional whole-cell patch-clamp)及細胞接觸穿孔記錄法(cell-attached perforated patch),記錄內皮細胞之電生理參數變化,我們的結果顯示:(1) 三種不同處理之HUVEC細胞膜之電生理參數各有差異; 包括四種不同I-V curve之電生理類型; (2) Histamine刺激使細胞膜電位過極化(hyperpolarization),能誘導一外向電流(outward current),且培養的細胞比新鮮分離之細胞對histamine刺激敏感; (3) 酵素處理似乎對細胞膜有所傷害使細胞對histamine之反應變遲鈍; (4) Acetylcholine對於三種不同處理之HUVEC均無誘發電流。

Endothelial cells lining the inner surface of blood vessels act as a transducing surface for many physiological functions, such as controlling the permeability of blood-tissue interface, adjusting the vascular diameter according to hemodynamic needs, and modulating of the function of white blood cells and platelets. In the last decade, many channels have been described in endothelial cells, which are generally regarded as non-excitable cells. These channels control various ion permeabilities and change membrane potential, and thus modulate electrochemical driving force for Ca2+ movements. Activation of endothelial cells is usually coupled to an elevation in cytosolic Ca2+. It has been reported that blocking of K+ or Cl- channels may influence extracelluar Ca2+ influx. In this study, we have applied both conventional whole-cell patch-clamp and cell-attached perforated-patch techniques to examine electrophysiological characteristics of cultured and freshly isolated human umbilical vein endothelial cells (HUVECs). Our results showed that: (1) electrophysiological characteristics in HUVEC were different among three preparations, and these cells could be divided into four different types according to their I-V curves; (2) histamine induced an outward current and caused membrane hyperpolarization, and the cultured cells were more sensitive to histamine treatment than freshly isolated cells; (3) collagenase treatment during HUVEC isolation might damage cell membrane and reduced its sensitivity to histamine; (4) none of these three HUVEC preparations showed acetylcholine-induced membrane currents.

中文摘要--------------1
英文摘要--------------2
緒論--------------------3
材料與方法----------6
結果------------------14
討論------------------27
參考文獻-----------32
附錄------------------36

Adams DJ, Barakeh J, Laskey R, Van Breemen C. Ions channels and regulation of intracellular calcium in vascular endothelial cells. FASEB J. 3: 2389- 2400, 1989

Azuma H, Ishikawa M, Sekizaki S. Endothelium-dependent inhibition of platelet aggregation. Br. J. Pharmacol. 88: 411- 415, 1986

Albelda SM, Buck CA. Intergrins and other cell adhesion molecules. FASEB J. 4: 2868-8880,1990

Bossu J, Feltz LA, Rodeau JL, Tanzi F. Voltage-dependent calcium transient currents in freshly dissociated capillary endothelial cells. FASEB Lett. 255: 377-380, 1989

Bregestovski P, Bakhramov A, Danilov S, Moldobaeva A, Takeda K. Histamine-induced inward currents in cultured endothelial cells from human umbilical vein. Br. J. Pharmacol. 95: 429- 436, 1988

Davies PF. Flow-mediated endothelial mechanotransduction. Physiol. Rev. 75: 519- 560, 1995

Delean A, Munson PJ, Rodbare D. Simultaneous analysis of families of sigmoidal curves: application to bioassay, radioligand assay, and physiological dose-response curves. Physiol. 235: E97- E102, 1978

Folkman J, Moscona A. Role of cell shape in growth control. Nature. 273: 345- 347, 1978

Gericke M, Oike M, Droogman G, Nilius B. Inhibition of capacitative Ca2+ entry by a Cl- channel blocker in human endothelial cells. European Journal of Pharmacology. 269: 381-384, 1994

Groschner K, Graier WF, Kukovetz WR. Histamine induces K+, Ca2+, and Cl- currents in human vascular endothelial cells. Circ Res. 75: 304-314, 1994

Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Archiv. 391: 85-100,1981

Himmel HM, Whorton AR, Strauss HC. Intracellular calcium, currents, and stimulus-response coupling in endothelial cells. Hypertension. 21: 112-127, 1993

Jing M, Frederick G, John L. Diaspirin cross-linked hemoglobin does not alter isolated human umbilical artery or vein tone. Art Cell, Blood Subs, & Immob Biotech. 24: 621-628, 1996

Killachey JJF, Johnston MG, Movat HZ. Increased permeability of microcarrier-cultured human endothelial monolayers in response to histamine and thrombin. Am. J. Pathol. 122: 50-61, 1986

Marchenko SM, S.O. Sage. Mechanism of acetylcholine action on membrane potential of endothelium of intact rat aorta. Am. J. Physiol. 266: H2388-H2359, 1994

Meyrick B & Brigham KL. Increase permeability associated with dilatation of endothelial cell junctions caused by histamine in intimal explants from bovine pulmonary artery. Exp. Lung Res.6: 11- 25 , 1984

Nilius B, Riemann D. Ion channels in human endothelial cells. Gen. Physiol. Biophys. 9: 89-112, 1990

Nilius B, Schwartz G, Oike M, Droogmans G. Histamine-activated, non-selective cation currents and Ca2+ transients in endothelial cells from human umbilical vein. Pflugers Arch. 424: 285-293, 1993

Nilius B, Viana F, Droogmans G. Ion channels in vascular endothelium. Annu. Rev. Physiol. 59: 145-170, 1997

Ogata N, Tatebayashi H. A simple and multi-purpose concentration-clamp method for rapid superfusion. Journal of Neuroscience Methods. 39: 175-183, 1991

Oike M, Gericke M, Droogmans G, Nilius B. Calcium entry activated by store depletion in human umbilical vein endothelial cells. Cell calcium. 16: 367-376, 1994

Ono K, Nakao M, Iijima T. Chloride-sensitive nature of the histamine-induced Ca2+ entry in cultured human aortic endothelial cells. Journal of physiology. 511: 837- 849, 1998

Rae J, Cooper K, Gates P, Watsky M. Low access resistance perforated patch recordings using amphotericin B. Journal of neuroscience Methods. 37: 15- 26, 1991

Revest PA, Abbott NJ. Membrane ion channels of endothelial cells. Tips. 13: 404-407, 1992

Rotrosen D, Gallin JI. Histamine type I receptor occupany increase endothelial cytosolic calcium,reduces F-actin, and promotes albumin diffusion across cultured endothelial monolayers . J Cell Biol. 103: 2379-2387, 1986

Rubanyi GM. Endothelium-derived relaxing and contracting factors. J Cell Biochem. 46: 27-36, 1991

Takeda K, Klepper M. Voltage-dependent and agonist activated ionic currents in vascular endothelial cells: A review. Blood Vessels. 27: 169- 183, 1990

Tracey WR, Peach MJ. Differential muscarinic receptor mRNA expression by freshly isolated and cultured bovine aortic endothelial cells. Circulation Research. 70: 234-240, 1992

Vanhoutte PM, Rubanyi GM, Miller VM, Houston DS. Modulation of vascular smooth muscle contraction by endothelium. Annu. Rev. Physiol 48: 307-320, 1986

Vargas FF, Caviedes PF, Grant DS. Electrophysiological characteristics of cultured human umbilical vein endothelial cells. Microvascular. 47: 153-165, 1994

Zunkler BJ, Henning B, Grafe M, Bass R, Hildebrandt AG, Fleck E. Electrophysiological properties of human coronary endothelial cells. Basic Res Cardiol. 90: 435- 442, 1995

QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
1. 石之瑜,「關於大陸少數民族的公民化」,共黨問題研究(台北),23卷3期,1997年3月,頁17-26。
2. 吳安家,「中共十四大修改黨章的政治意涵」,中國大陸研究(台北),36卷1期,1993年1月,頁5-13。
3. 吳安邦,「一九九七年中共少數民族問題」,中共研究(台北),32 卷1期,1998年1月,頁82-94。
4. 王章陵,「新疆地區少數民族分離運動的分析」,共黨問題研究(台 北),23卷3期,1997年3月,頁5-16。
5. 吳傳國,「中共少數民族政策及其困境」,國防雜誌(台北),13卷5期,1997年11月,頁47-60。
6. 宋國誠,「中共八屆人大二次會議後的發展情勢與對策」,中國大陸研究(台北),37卷5期,1994年5月,
7. 李志堯,「由南疆暴動看中共政權的隱憂」,國防雜誌(台北),12卷11期,1997年5月,頁47-52。
8. 李信成,「中共沿邊開放與新疆經貿發展」,共黨問題研究(台北),21卷7期,1995年7月,頁42-51。
9. 林文軒,「大陸少數民族地區社經發展概況與問題」,共黨問題研究(台北),21卷2期,1995年2月,頁74-88。
10. 俞劍鴻,「在後鄧小平時代-中國會出現區域性軍事分離主義嗎?」,中華戰略學刊(台北),1994年3月,頁 101-110。
11. 張麟徵,「分離主義的內省與外觀」,問題與研究(台北),33卷10期,1994年10月,頁1-21。
12. 畢英賢,「新疆的政情與改革開放」,中國大陸研究(台北),36卷10 期,1993年10月,頁38-47。
13. 黃友信,「中共的少數民族政策與社會控制」,共黨問題研究(台北),21卷5期,1995年5月,頁55-74。
14. 熊自健,「近年來中共的宗教政策」,中國大陸研究(台北),37卷7 期,1994年7月,頁35-44。
15. 熊坤新,「中國大陸伊斯蘭教現況」,中國邊政(台北),138期,1997年12月,頁43-47。