臺灣博碩士論文加值系統

許多臨床疾病的診斷已用唾液取代血液，血液中跟運動有關的某些生化值像乳酸、肌酸酐、血尿素氮、尿酸等，在唾液中也可測得這些運動生化值，只是濃度較低，本實驗之目的在找出唾液中與血液濃度成一定比例變化的運動生化值，進而將來能夠以簡單易得的唾液來取代抽血，以檢測運動員的運動生化值。本研究分兩部分，第一部分以18人(女生10人、男生8人)為對象，在休息狀態時採集唾液與血液，以國內運動訓練中心及學校常用的乾式自動血液分析儀分析唾液與血液中的乳酸、肌酸酐、血尿素氮、尿酸濃度，以觀察唾液與血液中的這些運動生化值是否具有相關性，實驗結果顯示唾液與血液中乳酸、肌酸酐、血尿素氮、尿酸濃度皆達到有意義的相關(p<0.05)，乳酸及肌酸酐濃度的r值依序為0.85及0.77，呈高度相關，而唾液與血液中血尿素氮及尿酸濃度的r值依序為0.62及0.50，其相關性較低。第二部分另以5人(女生3人、男生2人)為對象，以腳踏車測力器從事漸增負荷運動，在運動中及休息時連續採集唾液與血液，以乾式自動血液分析儀分析唾液與血液中的乳酸，以觀察唾液與血液中的乳酸濃度是否具有相關性，實驗結果顯示唾液與血液中乳酸濃度的r值皆大於0.94，因此在預測血液中乳酸及肌酸酐濃度方面，唾液不失為一項非侵入性、簡便的替代體液。

Saliva is used as a biological fluid to replace the blood in some clinical diagnoses. To get the saliva is more convenient and easier than that of blood. The present study is to investigate whether the blood can be substituted by saliva to determine the concentration of lactate, creatinine, blood urea nitrogen and uric acid. Eighteen subjects(10 female, 8 male) participated in the first part of this study. Saliva and blood were collected in the same time at rest. Samples were analyzed by a dry-type blood analyzer, which was the most frequently used blood analyzer in Taiwan sports society. Our results showed the higher correlation values of lactate(r=0.85) and creatinine(r=0.77) between saliva and blood(p<0.05). The correlation values of blood urea nitrogen(r=0.62) and uric acid(r=0.50) between saliva and blood also reached the significant level at α=0.05. Five subjects(3 female, 2 male) participated in the second part of this study. Saliva and blood were collected and analyzed at 5 minute intervals during the performance of a graded exercise test on a cycle ergometer against increasing workloads. The correlation of lactate between saliva and blood was all above 0.94. We suggest that saliva can be used as an alternative fluid to determine the lactate and creatinine.

第壹章 緒論
一.研究動機………………………………………………………… 1
二.研究目的………………………………………………………… 2
第貳章 文獻探討
一.唾液……………………………………………………………… 4
二.唾液的臨床診斷應用…………………………………………… 6
三.乳酸……………………………………………………………... 9
四.肌酸酐………………………………………………...………...11
五.血尿素氮………………………………………………………..12
六.尿酸…………………………………………………………......13
七.血液分析儀…………………………………………………13
第參章 材料與方法
一. 研究對象………………………………………………………. 14
二. 實驗時間及地點………………………………………………..14
三. 實驗使用的器材………………………………………………..14
四. 實驗設計與方法………………………………………………..15
五. 生化值的測量…………………………………………………..18
六. 資料收集與處理………………………………………………..20
第肆章 結果與討論
一. 受試者的測試結果及統計分析……………………… 21
二. 安定性測驗……………………………………………. 32
三. 再現性測驗……………………………………………. 33
第伍章、結論與建議
一. 結論……………………………………………………..35
二. 建議……………………………………………………..35
參考文獻……………………………………………………………......36

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