臺灣博碩士論文加值系統

甲基丙烯酸三團聯共聚物為多功能性的基材，本研究將此共聚物改質螯合金屬離子，藉由IMA系統的特性初步嘗試用以選擇性共沈澱特定蛋白質，達到純化分離的效果。此外，改質高分子如未接金屬離子，則以初步的動力學試驗推測應用於處理重金屬廢水的可行性，同時配合高分子的回收再利用，使應用上可以提高經濟效益。本研究重點可分為下面四點︰
( 1 ) 三團聯共聚物的改質：分別對於改質共聚物和蛋白質共沈澱實驗，及處理過渡金屬廢水的可行性研究方面選擇不同類型的三團聯共聚物。改質方面可分為三階段：高分子部份的醯氯化、高分子上醯氯化部份共價鍵結螯合觸手IDA、以IDA螯合過渡金屬離子。
( 2 ) 有關改質共聚物的化學鑑定部份：首先是高分子醯氯化後，利用FT-IR測定高分子是否有醯氯化發生，而高分子改質螯合觸手後，則使用NMR-H1和NMR-C13測定，初步定性了解改質高分子的改質程度和可能結構。
( 3 ) 改質共聚物的物理性質測定部份：
(a) 量測改質高分子的沈澱區，並藉此推測等電點。
(b) 以表面張力計測定改質高分子的臨界微胞濃度。
(c) 量測高分子平均分子量，並推測接上螯合觸手程度。
(d) 利用ICP-AES及AA定量測定改質共聚物螯合金屬
離子的程度和其能力。
( 4 ) B8M12A16-IDA-Cu和蛋白質初步共沈澱實驗：所選蛋白質表面分別具有不同數目的金屬親和點，對鍵結過渡金屬離子的改質高分子，具有不同程度之親和性。共沈澱實驗嘗試以鹽類遮蔽靜電作用力影響，調整適當的pH值以單純顯露專一親和作用力。初步結果顯示分子量14000以下，且表面金屬親和點較少的蛋白質Lysozyme、Ribonuclease A完全沒有發生親和性共沈澱，而分子量較高同時表面金屬親和點較多的Hemoglobin則在適當條件下有親和性共沈澱發生。此外也發現，Myoglobin在低pH時和螯合金屬之改質共聚物有不尋常共沈澱現象。
( 5 ) B8M12A32-IDA處理過渡金屬廢水的初步研究：由初步實驗可知廢水中重金屬離子可被改質高分子螯合而由廢水中分離出來。另外回收改質高分子和再鍵結金屬離子的實驗方面，結果發現高分子回收率和金屬再鍵結率很低，因此需對鍵結機構進行了解，以提昇回收的改質高分子的能力。

中文摘要 Ⅰ
英文摘要 Ⅲ
目錄 Ⅴ
表目錄 Ⅷ
圖目錄 Ⅸ
一、 緒論 1
二、 文獻回顧 3
2.1 團聯共聚物 3
2.1-1 團聯共聚物種類與合成方法 3
2.1-2 甲基丙烯酸系列團聯共聚物合成其特性 5
2.1-3 團聯共聚物的改質 8
2.2親和性共沈澱機制 9
2.2-1 選擇性共沈澱之高分子 9
2.2-2 選擇性共沈澱之配合基 11
2.3IMA系統 16
2.3-1IMA系統之親和性機構 16
2.3-2IMA系統之親和性應用 17
2.3-3IMA系統之影響因子 20
三、 研究目的和實驗流程 25
3.1研究目的 25
3.2實驗流程 27
四、 實驗裝置及藥品 28
4.1實驗藥品 28
4.2實驗裝置 30
五、 實驗步驟和分析方法 31
5.1團聯共聚物的純化 31
5.2團聯共聚物的改質 32
5.2-1酸基的醯氯化步驟 33
5.2-2鍵結觸手和純化的步驟 34
5.2-3鍵結過渡金屬和純化的步驟 36
5.3團聯共聚物的鑑定 37
5.3-1以FTIR定性鑑定醯氯化前後高分子 37
5.3-2以C13-NMR定性鑑定醯氯化前後高分子 37
5.3-3以H1-NMR定性鑑定醯氯化前後高分子 38
5.4團聯共聚物的性質量測 39
5.4-1改質高分子沈澱區和等電點測定 39
5.4-2改質高分子表面張力和臨界微胞濃度測定 40
5.4-3改質高分子之分子量測定 40
5.4-4ICP-AES測定改質高分子上金屬離子濃度 41
5.5蛋白質親和性共沈澱應用 42
5.6重金屬污染廢水之處理應用 44
六、 結果和討論
6.1團聯共聚物的鑑定 46
6.1-1以FTIR定性鑑定醯氯化前、後高分子 46
6.1-2以C13-NMR定性鑑定醯氯化前、後高分子 46
6.1-3以H1-NMR定性與半定量醯氯化前後高分子 46
6.2團聯共聚物的性質量測 47
6.2-1改質高分子沈澱區和等電點測定 47
6.2-2改質高分子表面張力和臨界微胞濃度測定 48
6.2-3改質高分子之分子量測定 49
6.2-4ICP-AES測定改質高分子上金屬離子濃度 50
6.3蛋白質親和性共沈澱應用 51
6.4重金屬污染廢水之處理應用 54
6.5結果圖形 55
七、結論和建議 76
八、參考文獻 77
九、附錄 82

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