交感神經摘除後，曾有報告指出會導致相關周邊器官胜月太 性神經纖維免疫組織化學反應的密度增加。而三叉神經節共同受到感覺性胜月太及自律性兒茶酚月安類神經的支配，因此自律性神經系統及一般感覺神經系統間可能存在著某些功能上的交互作用，但仍缺乏直接之解剖証據。本實驗旨在利用山葵過氧化氫酉每(horseradish peroxidase, HRP)逆向輸送標示特性，多巴胺β-羥基酉每( dopamine β-hydroxylase, DBH)免疫螢光技術，探討三叉神經節中交感性傳入神經纖維之來源處。實驗發現於大白鼠之三叉神經節注入HRP後12小時，受標示神經元位於同側頸上神經節吻部, 而於單側摘除頸上神經節後會導致同側三叉神經節中DBH顯著降低。另合併觀察P物質(substance P, SP)及抑鈣素基因相關胜月太(calcitonin gene related peptide, CGRP) 之免疫組織化學法等,則發現於單側摘除頸上神經節後30天，SP及CGRP在三叉神經節、尾段腦幹三叉神經脊髓核的中間部及尾部腹側區以及副三叉神經核等區域均有增加的表現;以上結果顯示三叉神經節中的交感性傳入神經來自頸上神經節，即顯示三叉神經節中具有被同側頸上神經節所支配之交感性傳入神經。本實驗結果將可作為自律神經系統調控一般感覺神經系統的明確解剖証據。

Sympathectomy had been shown to result in an increased density of fibers immunoreactive for sensory peptides in peripheral targets innervated by both sensory and sympathetic neurons . Trigeminal ganglion is the targets of both autonomic and sensory innervation which contains peptidergic and sympathetic catecholaminergic fibers. This suggests that there might exist anatomical and functional interactions between sympathetic and sensory systems. In this study, we examined the origins of sympathetic innervation on trigeminal ganglion by HRP retrograde transport technique and further studied the sympathetic innervation by examination of immunoreactivities for dopamine β-hydroxylase (DBH) , calcitonin gene related peptide (CGRP) and substance-P ( SP ) in bilateral trigeminal ganglia followed unilateral superior cervical ganglionectomy. HRP labeled neurons, which restrictedly located at rostral portion, were only found in the ipsilateral superior cervical ganglion (SCG). After unilateral SCG ganglionectomy, the ipsilateral trigeminal DBH immunostaining was dramatically decreased while CGRP and SP immunostaining in the ipsilateral trigeminal ganglion were elevated in response to SCG ganglionectomy. In the lower medulla, CGRP and SP immunostaining were increased in both interpolaris and caudal portion of ventral spinal trigeminal nucleus and in paratrigeminal nucleus. The data demonstrate that trigeminal sympathetic
VI
innervation was originated from ipsilateral SCG. These results may
provide anatomical evidence of autonomic modulation on sensory system.

目錄
目錄…………………………………………Ι
圖目錄……………………………………….Ⅲ
中文摘要………………………………………………………Ⅴ
英文摘要………………………………………………………Ⅵ
第一章 緒論……………………………………………………1
第一節 三叉神經節與頸上神經節…………………….1
第二節 三叉神經節與頸上神經節之交互關係……….4
第三節 總結…………………………………………….7
第二章 材料與方法 ………………………………………….8
第一節 實驗動物……………………………………….8
第二節 山葵過氧化氫酉每追蹤標示法………………… 8
第三節 頸上神經節摘除術…………………………….9
第四節 組織化學染色法……………………………….10
第五節 免疫組織化學染色法………………………….11
第三章 結果……………………………………………………14
第一節 HRP標示神經元在頸上神經節的位置………..14
第二節 DBH免疫螢光反應在三叉神經節的表現……..14
I
第三節 SP及CGRP免疫組織化學染色在三叉神經節的表現…15
第四節SP及CGRP免疫組織化學染色在腦幹中的表現……..15
第四章 討論……………………………………………………………17
第五章 結論 ………………………………………………………….21
第六章 參考文獻………………………………………………………22
圖表…………………………………………………………………….29
附錄…………………………………………………………………….42

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