(3.235.108.188) 您好!臺灣時間:2021/03/03 20:40
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

我願授權國圖
: 
twitterline
研究生:趙春香
研究生(外文):Chun-Hsiang Chao
論文名稱:大白鼠三叉神經節與頸上神經節間聯會關係之探討
論文名稱(外文):Neuroanatomical Connection Between the Superior Cervical Ganglion and Trigeminal Ganglion in Rats
指導教授:劉江川王順德
指導教授(外文):Jiang-Chun LiuShuen-De Wang
學位類別:碩士
校院名稱:國防醫學院
系所名稱:生物及解剖學研究所
學門:生命科學學門
學類:生物訊息學類
論文種類:學術論文
論文出版年:1999
畢業學年度:87
語文別:英文
論文頁數:54
中文關鍵詞:上頸神經節三叉神經節抑鈣素基因相關胜P物質
外文關鍵詞:superior cervical gangliatrigeminal gangliacalcitonin gene-related peptide(CGRP)substance P
相關次數:
  • 被引用被引用:0
  • 點閱點閱:110
  • 評分評分:系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
交感神經摘除後,曾有報告指出會導致相關周邊器官胜月太 性神經纖維免疫組織化學反應的密度增加。而三叉神經節共同受到感覺性胜月太及自律性兒茶酚月安類神經的支配,因此自律性神經系統及一般感覺神經系統間可能存在著某些功能上的交互作用,但仍缺乏直接之解剖証據。本實驗旨在利用山葵過氧化氫酉每(horseradish peroxidase, HRP)逆向輸送標示特性,多巴胺β-羥基酉每( dopamine β-hydroxylase, DBH)免疫螢光技術,探討三叉神經節中交感性傳入神經纖維之來源處。實驗發現於大白鼠之三叉神經節注入HRP後12小時,受標示神經元位於同側頸上神經節吻部, 而於單側摘除頸上神經節後會導致同側三叉神經節中DBH顯著降低。另合併觀察P物質(substance P, SP)及抑鈣素基因相關胜月太(calcitonin gene related peptide, CGRP) 之免疫組織化學法等,則發現於單側摘除頸上神經節後30天,SP及CGRP在三叉神經節、尾段腦幹三叉神經脊髓核的中間部及尾部腹側區以及副三叉神經核等區域均有增加的表現;以上結果顯示三叉神經節中的交感性傳入神經來自頸上神經節,即顯示三叉神經節中具有被同側頸上神經節所支配之交感性傳入神經。本實驗結果將可作為自律神經系統調控一般感覺神經系統的明確解剖証據。

Sympathectomy had been shown to result in an increased density of fibers immunoreactive for sensory peptides in peripheral targets innervated by both sensory and sympathetic neurons . Trigeminal ganglion is the targets of both autonomic and sensory innervation which contains peptidergic and sympathetic catecholaminergic fibers. This suggests that there might exist anatomical and functional interactions between sympathetic and sensory systems. In this study, we examined the origins of sympathetic innervation on trigeminal ganglion by HRP retrograde transport technique and further studied the sympathetic innervation by examination of immunoreactivities for dopamine β-hydroxylase (DBH) , calcitonin gene related peptide (CGRP) and substance-P ( SP ) in bilateral trigeminal ganglia followed unilateral superior cervical ganglionectomy. HRP labeled neurons, which restrictedly located at rostral portion, were only found in the ipsilateral superior cervical ganglion (SCG). After unilateral SCG ganglionectomy, the ipsilateral trigeminal DBH immunostaining was dramatically decreased while CGRP and SP immunostaining in the ipsilateral trigeminal ganglion were elevated in response to SCG ganglionectomy. In the lower medulla, CGRP and SP immunostaining were increased in both interpolaris and caudal portion of ventral spinal trigeminal nucleus and in paratrigeminal nucleus. The data demonstrate that trigeminal sympathetic
VI
innervation was originated from ipsilateral SCG. These results may
provide anatomical evidence of autonomic modulation on sensory system.

目錄
目錄…………………………………………Ι
圖目錄……………………………………….Ⅲ
中文摘要………………………………………………………Ⅴ
英文摘要………………………………………………………Ⅵ
第一章 緒論……………………………………………………1
第一節 三叉神經節與頸上神經節…………………….1
第二節 三叉神經節與頸上神經節之交互關係……….4
第三節 總結…………………………………………….7
第二章 材料與方法 ………………………………………….8
第一節 實驗動物……………………………………….8
第二節 山葵過氧化氫酉每追蹤標示法………………… 8
第三節 頸上神經節摘除術…………………………….9
第四節 組織化學染色法……………………………….10
第五節 免疫組織化學染色法………………………….11
第三章 結果……………………………………………………14
第一節 HRP標示神經元在頸上神經節的位置………..14
第二節 DBH免疫螢光反應在三叉神經節的表現……..14
I
第三節 SP及CGRP免疫組織化學染色在三叉神經節的表現…15
第四節SP及CGRP免疫組織化學染色在腦幹中的表現……..15
第四章 討論……………………………………………………………17
第五章 結論 ………………………………………………………….21
第六章 參考文獻………………………………………………………22
圖表…………………………………………………………………….29
附錄…………………………………………………………………….42

參考文獻
1. Aberdeen J, Milner P, Lincoln J, Burnstock G. Guanethidine sympathectomy
of mature rats leads to increase in calcitonin gene-related peptide and
vasoactive intestinal polypeptide-containing nerves. Neurosci.47:453-61, 1992.
2. Adams GG, Cellun JF. Neuroparalytic keratitis and the effect of cervical
sympathectomy following operative procedures for trigeminal
neuralgia. Scottish Medical J.32(3):86-88, 1987.
3. Alvarez FJ, Kavookjian AM, Light AR. Ultrastructural morphology,synaptic
relationships,and CGRP immunoreactivity of physiologically identified C
-fiber terminals in the monkey spinal cord. J.Comp.Neurol.329:472-490, 1993.
4. Amara SG, onas V, Rosenfeld MG, Ong ES, Eveans RM, Alternative RNA
processing in calcitonin gene expression generates mRNAs encoding different
polypeptide products. Nature 296:240-244, 1982.
5. Arbab MAR, Wiklund L, Delgado T, Svendgaard NA. Stellate ganglion
innervation of the vertebro-basillar arterial system demonstrated in the rat
with anterograde and retrograde WGA-HRP tracing. Brain Res.445:175
-180, 1988.
6.Barr ML, Kiernan JA. The Human Nervous System ,6th Ed.Lippincott-Raven
, 1993,pp.131-135,367-372.
7. Benarroch EE, Zollman PJ, Schmelzer JD, Nelson DK, Low PA.
Guanethidine sympathectomy increases substance-P concentration in the
superior sympathetic ganglion of adult rats. Brain Res. 584:305-8, 1992;.
8. Carvalho TL, Hoodson NP, Blank MA, Wilson PE, Mulderry PK, Bishop AE,
et al. Occurrence,distribution and origin of peptide-containing nerves of
guinea-pig and rat male genitalia and the effects of denervation on sperm
22
characteristics. J. Anat. 149:121-41,1986.
9.Carpenter MB. Core Text of Neuroanatomy,4th Ed. Baltimore : Willians &
Wilkins , 1991,pp.176-181.
10. Chan-Palay V. The paratrigeminal nucleus .I. Neurons and synaptic
organization. J.Neurocytol.7:405-418, 1978.
11. Chan-palay V. The paratrigeminal nucleus .II. Identification and inter
-relations of catecholamine axons ,indolamine axons ,and substance P
immunoreactive cells in the neuropil . J.Neurocytol.7:419-442, 1978.
12. Cole DF, Bloom SR, Burnstock G, Butler JM, McGregor GP, Safffrey MJ, et
al. Increase in SP-like immunoreactivity in nerve fibers of rabbit iris
and ciliary body one to four months following sympathetic denervation. Exp.
Eye Res. 37:191-7 , 1983.
13. Daphne AB, Catia S, Lawrence K. GAP-43 m RNA and calcitonin gene
related peptide m RNA expression in sensory neurons are increased
following sympathectomy. Brain Res.Bull. 42(1):39-50, 1997.
14. Denise VR, Hanisch UK, Quirion R. Neuroanatomical localization ,
pharmacological characterization and functions of CGRP, related peptides
and their receptors. Neurosci. Behav. Rev. 21(5):649-78, 1997.
15. Fristad I, Heyeraas KJ, Kvinnsland IH. Neuropeptide Y expression in the
trigeminal ganglion and mandibular division of the trigeminal nerve after
inferior alveolar nerve axotomy in young rats. Exper.Neurol.142:276
-286, 1996.
16. Gibson SJ, Polak JM, Bloom SR, Sabate IM, Mulderry PM, Ghhatei MA,
McGregor GP, Morrison JEB, Kelly JS, Evans RM, Rosenfeld MG.
Calcitonin gene-related peptide immunoreactivity in the spinal cord of
man and eight other species . J. Neurosci.4:3101-3111, 1984.
17. Henry JL. Effects of substance P on functionally identified units in cat spinal
cord. Brain Res.114:439-451, 1976.
23
18. Henry MA, Nousek-Goebal NA, Westrum LE. Light and electron microscopic
localization of calcitonin gene-related peptide immunoreactivity in lamina II
of the feline trigeminal pars caudalis/medullary dorsal horn:A qualitative
study. Synapse 13:99-107, 1993.
19. Hokfrlt T, Kellerth JO, Nilsson G, Pernow B. Experimental
immunohistochemical studies on the localization and distribution of
substance P in cat primary sensory neurons, Brain Res.100:235-252, 1975.
20. Hokfrlt T, Kellerth JO, Nilsson G, Pernow B. Substance P:localization in the
central nervous system and in some primary sensory neurons. Science
190:889-890, 1975.
21. Hsu SM, Raine L, Fanger H. The use of avidin-biotin-peroxidase complex
(ABC) in immunoperoxidase teckniques : A comparison between ABC and
unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29:579
-580, 1981.
22. Jacqueline J, Christopher VH, Yeo H. The central distribution of primery
afferent from the external eyelids,conjunctiva,and cornea in the
rabbit,studied using WGA-HRP and B-HRP as transganglionic tracers.
Expt.Neurol. 142:217-25, 1996.
23. Janig W, Kollmann W. The involvement of the sympathetic nervous system in
pain- possible neuronal mechanism. Drug Res. 34(II):1066-73, 1984.
24.Kandel ER, Jessell TS,Cchwartz JM. Principle of Neuroscience ,3rd Ed.
Connecticut. Appleton & Lange ,1991,pp.701-710.
25. Kessler J, Bell W, Black I. Interactions between the sympathetic and sensory
innervation of the iris. J. Neurosci.3:1301-1307, 1983.
26. Kilduff TS, Sharp FR, Heller HC. [14C] 2-deoxyglucose uptake in ground
squirrel brain during hibernation. J .Neurosci.2:143-157, 1982.
27. Kilduff TS, Sharp FR, Heller HC. Relative 2-deoxyglucose uptake of the
paratrigeminal nucleus increases during hibernation. Brain Res.262:117
24
-123,1983.
28. Kilduff TS, Sharp FR, Heller HC. Progressive activation of paratrigeminal
nucleus during entrance to hibernation. American J.Physiology 255:178
-181, 1988.
29. Kummer K. Sensory ganglia as a target of autonomic and sensory nerve
fibers in the guinea-pig. Neurosci. 59(3):739-54, 1994.
30. Kummer W, Gibbins IL, Stefan P, Kapoor V. Catecholamines and
catecholamine - synthesizing enzymes in guinea-pig sensory ganglia. Cell Tiss.
Res. 261:595 — 606, 1990.
31. Lembeck F. Sir Thomas Lewis’s nocifensor system,histamine and substance
-P-containing primary afferent nerves . Trends Neurosci.6:106-198, 1983.
32. Lindh B, Staines W, Hokfeit T, Terenius L, Salvaterra PM. Immunohisto-
chemical demonstration of choline acetyltransferase-immunoreactive
preganglionic nerve fibers in guinea pig autonomic ganglia. Proc.Natl.Acad
Sci.USA 83:5316-5320, 1986.
33. McLachlan EM, Janig W, Devor M, Michaelis M. Peripheral nerve injury
triggers noradrenergic sprouting within dorsal root ganglia. Nature
383:543-5,1993.
34. Marfurt CF. The central projections of trigeminal primary afferent neurons
in the cat as determined by the transganglionic transport of horseradish
peroxidase. J.Comp.Neurol.203:785-798, 1981.
35. Marfurt CF, Turner DF. Sensory nerve endings in the rat orofacial region
labelled by the anterograde and transganlionc transport of horseradish
peroxidase:A new method for tracing peripheral nerve fibers .Brain
Res.261:1-12, 1983.
36. Marfurt CF, Rajchert DM. Trigeminal primary afferent projections to “non
-trigeminal “ areas of the rat central nervous system. J.Comp.Neurol.303:489
-511, 1991.
25
37. Menetrey D, Leah J, Pommery J. Efferent projections of the paratrigeminal
nucleus in the rat. Neurosci.Lett.73:48-52, 1987.
38. Mesulam MM. Tetramethyl benzidine for horseradish peroxidase
neurochemistry: a non-carcinogenic blue reaction-product with superior
sensitivity for visualiing neural afferents and efferents. J.Histochem
Cytochem. 26:106-117, 1978.
39. Mineta Y, Morimoto M, Harano K, Totoki T. Sympathetic postganglinonic
innervation of external carotid artery,internal carotid artery,common carotid
artery and aorta in the dog-experimental study using HRP and WGA-HRP.
Masui- Jap. J. Anesthesiology 41(4):547-53, 1992.
40. Nilsson BY. Effects of sympathetic stimulation on mechanoreceptors of cat
vibrissae . Acta Physiol.Scand.68:127-128, 1972.
41. Noback CR, Strominger NL, Demarest RJ. The Human Nervous System,3rd
Ed.The National Book Company,1983,pp244-267.
42. Phelan K, Falls W. The interstitial system of the spinal trigeminal tract in the
rat :anatomical evidence for morphology and functional
heterogeneity . Somatosens.Mot.Res.6:367-399, 1989.
43. Quigg M, Elfvin LG, Aldskogius H. Distribution of cardiac sympathetic
afferent fibres in the guinea pig heart labeled by anterograde transport of
wheat germ agglutinin-horseradish peroxidase. J. Auton Nerv. Syst.25:107-
18, 1988.
44. Santini M. Adrenergic fibres in the feline Gasserian ganglion. Life Sci.5:283-
7, 1966.
45. Skofittsch G, Jacobowitz DM. Calcitonin gene-related peptide coexists with
substance-P in capsaicin sensitive neurons and sensory ganglia of the
rat. Peptides 6:747-754, 1985.
46. Stevens RT, Hodge CJ, Apkarian AV. Catecholamine varicosities in cat
dorsal root ganglion and spinal ventral roots. Brain Res. 261:151-154, 1983.
26
1986.
47. Stone RA, Kuwayama Y, Terenghi G, Polak J.M. Calcitonin gene-related
peptide : occurrence in corneal sensory nerves. Expt. Eye Res. 43(2):279-283,
48. Sugimoto T, Fujiyoshi Y, Xiao C, He YF,Ichikawa H. Central projection of
calcitonin gene-related peptide (CGRP)-and substance P(SP)-immunoreactive
trigeminal primary neurons in the rat. J.Comp.Neurol. 378:425-442, 1997.
49. Takahashi T, Otsuka M. Regional distribution of substance P in the spinal
cord and nerve roots of the cat and the effect of dorsal root section. Brain
Res.87(1):1-11,1975.
50. Takemura M, Sugimoto T, akai A. Topographic organization of central
terminal region of different sensory branches of the rat mandibular
nerve. Exper.Neurol.96:540-557, 1987.
51. Takemura M, Sugimoto T, Shigenaga Y. Difference in projection of primary
afferents innervating facial and intraoral structures in the rat. Exper. Neurol.
111:324-331, 1991.
52. Terenghi G, Polak JM, Rodrigo J, Mudrry PK, Bloom SR. Calcitonin gene
-related peptide immunoreactive nerves in the tongue,epiglottis and pharynx
of the rat : occurrence,distribution and origin. Brain Res.365:1-14, 1986.
53. Terenghi G, Zhang S-Q, Unger WG, Polak JM. Mophological changes of
sensory CGRP-immunoreactive and sympathetic nerves in peripheral tissues
following chronic denervation. Histochem. 86:89-95, 1986.
54. Traub RJ, Allen B, Humphrey E, Ruda MA . Analysis of calcitonin gene
-related peptide-like immunoreactivity in the cat dorsal spinal cord and
dorsal root ganglia provide evidence for a multisegmental projection of
nociceptive C-fiber primary afferents. J.Comp. Neurol.302:562-574, 1990.
27
55. Unger WG, Terenghi G, Ghatei M, Ermis K, Butler J, Zhang S, et al.
Calcitonin gene-related polypeptide as a mediator of the neurogenic ocular
injury response. J. Ocular Pharmacol. 1:189-99, 1985.
56. Van Ranst L, Lauwweryns J. Effects of long-term sensory vs sympathetic
denervation on the distribution of calcitonin gene-related peptide and
tyrosine hydroxylase immunoreactivities in the rat lung. J. Neuroimmunol.
29:131-8,1990.
57. Walker MW, Ewald DA, Perney TM, Miller RJ. Neuropeptide Y modulates
neurotransmitter release and calcium currents in rat sensory neurons.
J. Neurosci.8:2438-2446, 1988.
58. Yodlowski ML, Fredieu JR, Landis S. Neonatal 6-hydroxydopamine
treatment eliminates cholinergic sympathetic innervation and induces
sensory sprouting in rat sweat glands. J. Neurosci. 4:1535-48, 1984.

QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
系統版面圖檔 系統版面圖檔