美洲蜚蠊 Periplaneta americana L.的性費洛蒙一直被認為是在消化道的中腸產生的。近來尚有研究報告指出在雌蟲腹末的腔腺收集到大量之主要性費洛蒙蜚蠊酮乙。本文報告以生物檢定法重新檢查美洲蜚蠊未交尾雌蟲性費洛蒙的產生部位的結果。雌體多處組織的萃取液會使未交尾雄蟲產生性反應，其中以包括結腸和直腸的後腸引起的反應最強，尤以結腸的反應較強。以觸角電析法比較未交尾雌蟲腔腺、肛門、直腸、結腸個別的萃取液對未交尾雄蟲觸角的反應，以結腸引起之觸角電析反應最強。經氣相色層分析比對，發現結腸內性費洛蒙有0.34 ng的蜚蠊酮甲和8.31 ng的蜚蠊酮乙。逐一解剖正在求偶的未交尾雌蟲，比較其結腸和腔腺萃取物對雄蟲引發之性反應，結果結腸的反應最好，腔腺幾乎完全沒有反應。有關美洲蜚蠊性費洛蒙的出處，本研究與前人報導相異之處，亦在文中討論。

For a long time, the midgut has been proposed to be the sex pheromone production site in the female American cockroach (Periplaneta americana L.). A recent report indicates that atrial glands at the abdominal tip produce large quantity ofthe main sex pheromone component, periplanone-B. The study identifies sex pheromone production site in the female American cockroach using bioassay with the extracts of variousfemale body parts which aroused sexual responses from the unmated males. The extract of hindgut, including both colonand rectum, induced strong sexual responses from males; moreover, colon extract was stronger. The electroantennogramof male antennae in response to the contents of atrial glands,pygidium, rectum and colon showed that colon extract was more effectiveness. From gas chromatographic analysis, the quantity of periplanone-A (PA) and periplanone-B (PB), the main sex pheromone components in female American cockroach, was 0.34 and 8.31 ng, respectively, in the colon of a virgin female. The ratio of PA to PB was ca. 1:24. Based on these studies, colon was shown to be the main sex pheromone source in the virgin female American cockroach. The reason why present results differed from those previous studies was also discussed in the content.

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