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研究生:方慰親
研究生(外文):Wei-Ching Fang
論文名稱:鐵對水稻切離葉片生理作用影響之研究
論文名稱(外文):Studies on the physiological effects of iron on detached rice leaves
指導教授:高景輝高景輝引用關係
指導教授(外文):Ching Huei Kao
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:農藝學研究所
學門:農業科學學門
學類:一般農業學類
論文種類:學術論文
論文出版年:1999
畢業學年度:87
語文別:中文
論文頁數:83
中文關鍵詞:水稻
外文關鍵詞:Oryza sativairon
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本論文係以水稻台中在來一號 (Oryza sativa L., cv. Taichung Native 1) 為試驗材料,探討鐵對水稻切離葉片生理作用之影響。主要包括三個部份:(一)硫酸亞鐵造成水稻切離葉片毒害之可能機制,(二)鐵離子與MJ所促進的水稻切離葉片老化之關係,與(三)鐵離子增加水稻切離葉片POD活性之可能機制。
過量的硫酸亞鐵處理會導致蛋白質與葉綠素含量的下降,且提高MDA之含量。過量的硫酸亞鐵處理會誘導APOD與GR活性上升,SOD活性下降。自由基清除劑 (manntiol、ascorbate、GSH) 可降低硫酸亞鐵所造成之毒害,顯示硫酸亞鐵之毒害係由活化氧族所造成。
鐵離子螫合劑可抑制MJ所造成的蛋白質與葉綠素含量之下降及脂質過氧化作用之增加。鐵離子螫合劑BP可降低MJ所促進的老化作用之機制可能是與Fe2+ 或Cu2+ 結合,降低Haber-Weiss或Fenton反應之進行。BP前處理可增加切離葉片內SOD之活性,故BP之作用亦可能與SOD活性的增加有關。
硫酸亞鐵處理,無論在光線或黑暗下皆會誘導POD活性之增加。POD活性之增加與自由基無關,可能與蛋白質重新合成有關。另外,Cu2+ 或Zu2+ 也會誘導POD活性之上升,其也可能是因為蛋白質重新合成之故。而Fe2+,Cu2+ 或Zu2+ 處理皆會造成POD同功酵素圖譜之改變。
The aim of this thesis was to study the effects of iron on the physiology of detached rice (Oryza sativa L., cv. Taichung Native 1) leaves. We investigated (a) the possible mechanism of FeSO4-induced toxicity of detached rice leaves, (b) the possible involvement of Fe2+ in MJ-promoted senescence of the detached rice leaves, (c) the possible mechanism of Fe2+-induced peroxidase (POD) activity in detached rice leaves.
Excess FeSO4 decreased the levels of protein and chlorophyll, and increased lipid peroxidation. Excess FeSO4 treatment also resulted in an increase in the activities of APOD and GR and a decrease in the activity of SOD. Free radical scavengers (mannitol, ascorbate or GSH) were able to alleviate the toxicity induced by FeSO4, indicating that FeSO4-induced toxicity could be mediated through the action of activated oxygen.
The iron chelators such as BP, HQ and PA were effective in alleviating MJ-promoted senescence of detached rice leaves. Among them, BP was the most effective in reducing MJ-promoted senescence. The effect of BP in reducing MJ-promoted senescence is most likely due to its chelation of iron or copper, which results in the reduction of Fenton or Haber-Weiss reaction, and an increase in SOD activity.
POD activity in detached rice leaves was significantly induced by FeSO4 under both light and dark conditions. FeSO4-induced POD activity is not attributed to free radicals, but is due to inducing de novo synthesis of the enzyme. Moreover, Cu2+ and Zn2+ can also induce POD activity. The induction of POD activity by Cu2+ or Zn2+ is also due to de novo synthesis of the enzyme. Results of IEF gel indicated that isozyme pattern of POD changed in Fe2+, Cu2+ or Zn2+-treated detached rice leaves.
一、前言
二、前人研究
三、材料與方法
四、結果
五、討論
六、引用文獻
七、中文摘要
八、英文摘要
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