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研究生:劉威炬
論文名稱:含多氯聯苯河底倪中厭氧微生物分解之研究
指導教授:張碧芬張碧芬引用關係
指導教授(外文):Chang Bee-Ven
學位類別:碩士
校院名稱:東吳大學
系所名稱:微生物學系
學門:生命科學學門
學類:微生物學類
論文種類:學術論文
論文出版年:1999
畢業學年度:87
語文別:中文
中文關鍵詞:多氯聯苯厭氧生物降解脫氯作用河底泥生物復育
外文關鍵詞:Polychlorinated biphenyls (PCBs)anaerobic degradationdechlorinationriver sedimentbioremediation
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多氯聯苯是美國及臺灣環保署優先列管毒性物質,可能存在河川底泥的環境中。本研究選取基隆河中下游,可能造成污染之6個採樣點添加多氯聯苯,探討河底泥中多氯聯苯之厭氧生物降解。結果顯示不同採樣點,河底泥之脫氯速率為K10(0.0075 mg/L/day) > K8(0.0070 mg/L/day) > K9(0.0066 mg/L/day) > K7(0.0041 mg/L/day) > K6(0.0040 mg/L/day)。2,3,4,5-CB在河底泥中添加具有脫氯活性之厭氧混合菌與否皆有脫氯效果。pH值在7~8,溫度在30~40℃,2,3,4,5-CB,2,3,5,6-CB及2,3,4,5,6-CB三種多氯聯苯呈現較佳的脫氯效果。三種多氯聯苯在濃度為1~20 mg/L,當濃度愈高時,有愈高之脫氯速率。
2,3,4,5-CB,2,3,4,5,6-CB及2,3,5,6-CB的脫氯過程並檢測到中間代謝產物。2,3,4,5-CB之代謝產物為2,4,5-CB、2,3,5-CB及2,5-CB;2,3,5,6-CB之代謝產物為2,3,6-CB及2,5-CB而2,3,4,5,6-CB之代謝產物為2,3,5,6-CB、2,3,4,5-CB、2,3,6-CB、2,4,6-CB及2,5-CB,至於二氯以下產物則未被偵測到。河底泥分解多氯聯苯快慢為2,3,4-CB(0.0701 mg/L/day)> 2,3,4,5-CB(0.0388 mg/L/day)> 2,3,4,5,6-CB(0.0145 mg/L/day)> 2,2',3,4,4',5,5'-CB, 2,2',3,3',4,4,',5,5'-CB, 2,3,5,6-CB, 2,2',4,4',6,6'-CB(0.0055~0.006 mg/L/day)> 2,2',3,3',4,4'-CB(0.003 mg/L/day)。混合上述八種多氯聯苯脫氯速率則更低。
比較不同還原狀態之脫氯速率,以甲生成狀態最快,硫酸還原狀態次之,脫氮狀態最慢。添加電子接受者如氯化鐵會抑制脫氯,,氧化錳則對脫氯有延滯現象。添加電子提供者醋酸鹽、乳酸鹽及丙酮酸鹽則有促進的影響。添加非離子型界面活性劑之結果顯示,添加Brij 30、SDS及SN 70中可抑制脫氯。而甲烷菌抑制劑(BESA)、硫酸還原抑制劑(molybdate)的添加皆會抑制脫氯的作用,顯示本研究微生物脫氯作用是以甲烷生成及硫酸還原狀態之菌群為主。
本研究結果可建立最適合之脫氯條件,作為含多氯聯苯河底泥生物復育之參考。
Polychlorinated biphenyls (PCBs) are priority-pollutants by U.S. and Taiwan EPA. It may be detected in river sediment. In this study, we investigated potential of anaerobic degradation of PCBs in sediment, which collected from six monitoring sites of middle and down stream of Ken-Lung river sediment. The results showed dechlorination rates of six sampling sites in the following order: K10(0.0075 mg/L/day) > K8(0.0070 mg/L/day) > K9(0.0066 mg/L/day) > K7(0.0041 mg/L/day) > K6(0.0040 mg/L/day). It had no significant difference on 2,3,4,5-CB dechlorination in river sediment whether HCB-adapted consortium was added or not. The optimal conditions for 2,3,4-CB, 2,3,4,5-CB and 2,3,4,5,6-CB dechlorination were 30℃~40℃ and pH7.0~8.0. At a concentration of 1 to 20 mg/L, the higher the three PCBs congeners concentration, the faster the dechlorination rates.
The intermediate products for 2,3,4,5-CB were 2,3,5-CB, 2,4,5-CB, and 2,5-CB ; 2,3,5,6-CB, 2,3,4,5-CB, 2,3,6-CB, 2,3,5-CB, 2,4,5-CB and 2,5-CB for 2,3,4,5,6-CB; 2,3,6-CB and 2,5-CB for 2,3,5,6-CB. PCBs congeners dechlorination rates were in the following order: 2,3,4-CB>2,3,4,5-CB>2,3,4,5,6-CB>2,2',3,3'4,4'-CB>2,2',4,4',6,6'-CB > 2,3, 5,6-CB>2,2', 3,4,4',5,5'-CB>2,2',3,3',4,4',5,5'-CB. Dechlorination rates were decreased by adding the eight PCBs congeners in mixture.
Under different anaerobic conditions, we found that 2,3,4,5-CB, 2,3,5,6-CB, and 2,3,4,5,6-CB dechlorination rates were in following order: methanogenic conditions>sulfate reducing conditions>denitrifying conditions. The three PCBs congeners were enhanced by the addition of lactate, pyruvate and acetate, but delayed by addition of manganese oxide and inhibited by addition ferric chloride. Above the critic micellar concentration (CMC) of non-surfactants such as Brij30, Brij35, Triton X-100 and Triton N-101, three PCBs congeners dechlorination rates was inhibited. Treatment microbial inhibitors such as bromoethane sulfonic acid and molybdate showed methanogen and sulfate-reducing bacteria involved in dechlorination.
From our results, we can establish the optimal dechlorination conditions which could be used for further application of bioremediation for clean up of PCBs—contaminated river sediment.
中文摘要
英文摘要
目錄
表目錄
圖目錄
前言
材料與方法
結果
討論
結論
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