臺灣博碩士論文加值系統

本試驗之目的係自梅花鹿腦垂腺 cDNA 基因庫選殖梅花鹿之泌乳素 cDNA。萃取自梅花鹿腦垂腺之總 RNA，利用反轉錄使之成為cDNA，再以 PCR 的方法擴增之，並再將此cDNA兩端加以修飾，接上EcoRI 的切位，嵌入λgt11 噬菌體之DNA 中，以此方式完成梅花鹿腦垂腺 cDNA 基因庫之構築，以梅花鹿泌乳素cDNA 835 bp 當探針，篩選完整之泌乳素基因。本試驗所得之泌乳素 cDNA與山羊相比較後發現，兩者之胺基酸長度相同，皆含有30個胺基酸之訊號與199個胺基酸之成熟。梅花鹿與山羊在訊號有一個胺基酸不同，而成熟中有10個胺基酸不同，其胺基酸之相似性為94.8%，核酸之相似性為95.2%。在基因的表現試驗中，將鹿泌乳素成熟之cDNA，接入 pTrc99A 原核表現載體中，使菌體大量表現梅花鹿泌乳素重組蛋白質，經西方吸漬法證實可產生一分子量為 23 kDa 之泌乳素。利用RT-PCR的方法分析，顯示腦垂體為唯一表現泌乳素基因之組織。

This study was attempted to clone prolactin cDNA from pituitary cDNA library of sika deer. Total RNA was extracted from pituitary of sika deer then the cDNA library was constructed. The full length of prolactin cDNA was screened by using 835 bp of prolactin PCR product as screening probe. The coding region of prolactin cDNA from sika deer showed high homology to that from goat with 95.2% homology in nucleotide sequence and 94.8% homology in amino acid sequence. The amino acid sequence of pituitary prolactin from sika deer deduced from cDNA suggested that it is consisted of 30 amino acids of signal peptide and 199 amino acid of mature peptide. Moreover, one amino acid from signal peptide and 10 amino acids from mature peptides were different between sika deer and caprine. The coding region of prolactin cDNA was then subcloned into prokaryotic expression vector pTrc99A and the recombinant prolactin was expression in E. coli . The result from Western Blot analysis showed that the molecular weight of this recombinant prolactin is 23 kDa. Analysis by RT-PCR showed that the pituitary is the only tissue expressing prolactin gene.

壹、 摘要…………………………………………………………………………………………1
貳、 前言…………………………………………………………………………………………2
參、 文獻檢討…………………………………………………………………………………4
一. 泌乳素之研究歷史……………………………………………………….………… 4
二. 泌乳素的基因序列及蛋白質構造……………………… ……………………7
(一) 泌乳素之蛋白質構造與cDNA………………… ……… ………………7
(二) 泌乳素基因組DNA 之序列………………… …………………………10
(三) 醣化的變異………………………………………… ………… … …………… 12
(四) 泌乳素的裂解產物…………………………………… …… ……… ………13
(五) 泌乳素家族之其他成員…………………………… ……… …… ……… 13
三. 泌乳素之合成與調節………………………………………………………………16
(一) 泌乳素的轉錄與合成路徑…………… …… …… ……………………… 16
1. 轉錄作用………………………… ………………… ………… …………………16
2. 泌乳素的生物合成……………………… ……… ……… …… …………… 18
(二) 泌乳素分泌的內分泌控制……………………………… ………………… 19
1. 泌乳素抑制的因子……………………… …… ………………………………19
(1 ) 度巴胺………………………… ………………………………………………19
(2 ) 內皮素………………………………… ………………… ……………………21
( 3 ) 降鈣素…………………………………………………………………… 21
2 . 泌乳素釋放因子………… …………………………………………………22
( 1 ) 激甲狀腺素…………………………………………………………… 22
( 2 ) 小腸收縮………………………………………………… …… ……23
肆、 材料與方法……………………………………………………………………………25
伍、 結果……………………………………………………………….………………………44
陸、 討論………………………………………………… ……………………………………53
柒、 結論…………………………………………… …… ……………………………………61
捌、 參考文獻…………………………………… …… …… ………………………………62
玖、 英文摘要…………………………………………………………………………………75

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