本試驗分別以甲硫胺酸鋅、離胺酸鋅、硫酸鋅或氧化鋅四種鋅來源，添加於低鋅飼糧中飼餵生長豬，以測定生長豬對不同鋅來源之利用率。試驗採2×5×5重複拉丁方格設計，五頭30公斤（試驗一）及五頭60公斤（試驗二）之杜洛克×藍瑞斯雜交閹公豬，分兩個試驗，五個階段分別餵飼五種試驗飼糧，並以體重3.5% 為每階段之每日飼料餵飼量，試驗處理分別為：1. 以玉米－大豆粕為主配成低鋅（30 mg/kg）之基礎飼糧組（B）（對照組）；2. B額外再添加甲硫胺酸鋅提供鋅60 mg/kg組，3. B額外再添加離胺酸鋅提供鋅60 mg/kg組，4. B額外再添加硫酸鋅提供鋅60 mg/kg組及5. B額外再添加氧化鋅提供鋅60 mg/kg組。飼糧中添加0.25%三氧化二鉻作指示劑。豬隻飼養於不鏽鋼代謝架中，試驗一豬隻以外科手術於迴腸末端裝置T型管，收集腸道內容物，試驗二則無。每階段適應期為4天，收集期3天，每日收集豬糞及腸道內容物2次，以測定豬隻對鋅之消化率。結果顯示，在全腸道鋅消化率方面，除了未添加鋅組偏低（P＜0.05）外，添加鋅來源各組間差異不顯著。在不同鋅來源中鋅消化率方面，除了硫酸鋅中鋅消化率（ 10.5 % ）低於甲硫胺酸鋅及氧化鋅者（P＜0.05）外，而三組鋅來源中鋅消化率差異不顯著。甲硫胺酸鋅為22.5 %，離胺酸鋅為19.3 %，氧化鋅為22.5 %。在迴腸末端鋅消化率方面，不同飼糧及鋅來源中，鋅的消化率皆呈現負值（表三）。在不同飼糧中鋅消化率方面，四種鋅來源消化率皆呈現負值(-178∼-253 % )，Zn-Met組飼糧中鋅消化率顯著低於其他各組（P < 0.05）。在不同鋅來源中鋅消化率方面，四種鋅來源中鋅消化率間則差異不顯著。綜合上述結果顯示，豬隻對硫酸鋅中鋅之利用率偏低，而對甲硫胺酸鋅、離胺酸鋅及氧化鋅中鋅之利用率則相近。以全糞法測定鋅利用率即可。另外，試驗效應具顯著性（P < 0.01），此結果可能顯示，隨年齡的增長，豬隻對鋅的消化率隨之降低。

Two 5×5 Latin square designed experiments were conducted to determine the utilization of Zn from Zn-methionine, Zn-lysine, ZnSO4 and ZnO by 5 growing pigs at initial weight 30 kg for experiment 1, and initial weight 60 kg for experiment 2. Sixty ppm of Zn from above mentioned Zn compounds were added to the basal diet containing 30 ppm of Zn. Pigs were fed in the metabolism cage and the feces were collected for 3 days during each of 5 collection period. Cr2O3 was added in the diet as the marker for the digestibility study. Zn addition significantly increased the Zn digestibility of the diets ( P < 0.05 ). Zn digestibility in Zn-methionine, Zn-lysine and ZnO was similar, and ranged from 19 to 23 %, while Zn digestibility in ZnSO4 ( 10.5 % ) was much lower than of them ( P < 0.05 ). Zinc digestibility is inversely related to age ( P < 0.01 ).

誌謝 -------------------------------------------------------- 1
目次 -------------------------------------------------------- 2
表次 -------------------------------------------------------- 4
圖次 -------------------------------------------------------- 5
摘要 -------------------------------------------------------- 6
緒言 -------------------------------------------------------- 8
文獻檢討 ---------------------------------------------------- 9
壹﹑鋅之化學特性及其分布 --------------------------------- 10
貳﹑豬隻對鋅之需求 --------------------------------------- 11
一、 鋅之來源 ---------------------------------------------- 12
二、 鋅在動物體內之消化吸收 -------------------------------- 13
三、 鋅之排泄 ---------------------------------------------- 18
四、 鋅之生理功能 ------------------------------------------ 19
五、 鋅缺乏對豬隻之影響 ------------------------------------ 24
六、 鋅過量所引起的毒性反應 -------------------------------- 25
七、 鋅需要量 ---------------------------------------------- 26
參﹑豬隻對飼糧中鋅之可利用性
一、 測定飼糧中鋅利用性之方法 ------------------------------ 27
二、 不同飼糧成份對鋅可利用性之影響 ------------------------ 28
三、 不同鋅來源中鋅之可利用性 ------------------------------ 29
肆﹑豬糞中鋅對環境之影響
材料與方法 ------------------------------------------------- 36
結果 ------------------------------------------------------- 41
討論 ------------------------------------------------------- 48
結論 ------------------------------------------------------- 52
參考文獻 --------------------------------------------------- 53
英文摘要 --------------------------------------------------- 78
小傳 ------------------------------------------------------- 79

行政院農業委員會。1990。台灣地區飼養標準：豬。
AOAC. 1984. Official Methods of Analysis (14th Ed. ). Association of official Analytical Chemist, Arlington, VA.
Cousins, R. J. 1985. Absorption, transport, and hepatic metabolism of copper and zinc: special reference to metallothionein and ceruloplasmin. Phys. Rev. 65: 238 - 309.
Ellis, R., E. R. Morris, and A. D. Hill. 1982. Bioavailability to rats of iron and zinc in calcium-iron-phytate and calcium-zinc-phytate complexes. Nutr. Res. 2:319 - 322.
Fordyce, E. J., R. M. Forbes, K. R. Robbins, and J. W. Erdman, Jr. 1987. Phytate×calcium/zinc molar ratios: Are they predictive of zinc bioavailability? J. Food Sci. 52:440 - 444.
Hahn, J. D., and D. H. Baker. 1993. Growth and plasma zinc responses of young pigs fed pharmacologic levels of zinc. J. Anim. Sci. 71: 3020 - 3024.
Hill, D. A., E. R. Peo, Jr., A. J. Lewis, and J. D. Crenshaw. 1986. Zinc-amino acid complexes for swine. J. Anim. Sci. 63: 121 - 130.
Kratzer, F. H., and P. Vohra. 1986. Chelates in Nutrition. CRC Press. Boca Raton, FL. pp. 3.
NRC. (1988). Nutrient Requirements of Swine ( 9th Ed. ). National Academy Press. Washington, DC.
SAS. 1990. SAS/STAT User's Guide (Release 6.08). SAS Inst. Inc., Cary, NC.
Schell, T. C., and E. T. Kornegay. 1996. Zinc concentration in tissues and performance of weanling pigs fed pharmacological levels of zinc from ZnO, Zn-methionine, Zn-lysine, or ZnSO4. J. Anim. Sci. 74: 1584 - 1593.
Schwarz, F. J., and M. Kirchgessner. 1976. Distribution and excretion of intravenously administered zinc in rats with different zinc intake. Zentralblatt Fur Veterinarmedizin - Reihe A 23: 836 - 848.
Swinkels, J. W., E. T. Kornegay, W. Zhou, M. D. Lindemann, K. E. Webb, Jr., and M. W. Verstegen. 1996. Effectiveness of a zinc amino acid chelate and zinc sulfate in restoring serum and soft tissue zinc concentrations when fed to zinc-depleted pigs. J. Anim. Sci. 74: 2420 - 2430.
Wedekind, K., G. Collings, J. Hancock, and E. Titgemeyer. 1994. The bioavailability of zinc-methionine relative to zinc sulfate is affected by calcium level. Eighty Third Annual Meeting of the Poultry Science Association, Inc., Starkville, Mississippi, USA, August 73: 114.
Wedekind, K. J., A. E. Hortin, and D. H. Baker. 1992. Methodology for assessing zinc bioavailability: efficacy estimates for zinc-methionine, zinc sulfate, and zinc oxide. J. Anim. Sci. 70: 178 - 187.
Wedekind, K. J., A. J. Lewis, M. A. Giesemann, and P. S. Miller, 1994. Bioavailability of zinc from inorganic and organic sources for pigs fed corn-soybean meal diets. J. Anim. Sci. 72: 2681 - 2689.
Weigand, E., and M. Kirchgessner. 1979. Absorbability of zinc from different compounds. Zeitschrift fur Tierphysiologie, Tierernahrung und Futtermittelkunde 42: 137 - 146.
Williams, C. H., D. J. David, and D. Ilsmaa. 1962. The determination of chromic oxide in faeces samples by atomic absorption spectrophotometry. J. Agric. Sci. 59: 381 — 385.