臺灣博碩士論文加值系統

苯胺和其他芳香環胺類在工業上是很重要的原料，這些化合物通常被使用於染料、聚合物及農藥的製程上。苯胺對是一種疑似致癌物，並且會對環境造成污染。苯胺本身的氧化峰的電位在+760mV，但是在利用電化學方法直接偵測苯胺時，由於電極表面的聚合反應；阻礙了電子傳導，所以會使偵測的訊號越來越小，導致偵測再現性變差。本研究為利用流注分析系統，配合碳墨的使用來將phenothiazine修飾在電化學偵測器上，phenothiazine在被修飾的電化學偵測器上可以有效地將苯胺的偵測電位由+760mV降至+300mV，也可以避免掉聚合反應而造成的影響增加再現性。結果顯示連續測定苯胺10μM的氧化訊號之相對標準偏差為2.6%，偵測極限為0.1μM(S/N=3)，線性達20μM（相關係數R＝0.9997）。

Aniline and its derivatives are very important compounds in industrial application. Usually these compounds are used to produce dyestuff , polymers and pesticides etc. These derivatives have been suspected as a carcinogen among the environment pollutants. Aniline can be oxidized on +760 mV (Ag/AgCl). However, the direct oxidation of aniline can be troublesome as the polymerization effect on electrode surface when use electrochemical method. Because the polymerization obstructs electron transfer to electrode surface, thus, signal will decrease and cause poor reproducibility. A phenothiazine modified carbon ink electrode was used to detect aniline. The modified electrode can avoid polymerization effect and apply good reproducibility. At the measurement of 10μM aniline, the relative standard deviation is 2.6%(n=20). The estimated detection limit (S/N=3) is 0.1μM and the linearity of response is up to 20μM (with correlation coefficient R=0.9997)

圖表目錄…………………………………………………………III
1-1前言…………………………………… 1
1-2苯胺之應用範圍與其毒性……………………………………1
1-2-1苯胺的應用範圍…………………………………………….1
1-2-2苯胺的毒性………………………………………………….2
1-3苯胺的偵測…………………………………………………….3
1-3-1非電化學的偵測方法………………………………………..3
1-3-2電化學的偵測方法…………………………………………..4
1-4電極修飾的方法……………………………………………….7
1-4-1單層分子(Monomolecuar layers) ……………………………8
1-4-2共價鍵結(Formation of covalent bond between electrode and
elecrtroactive reagent) ……………………………………….9
1-4-3聚合物膜(polymer film coating on electrode) ……………….…………………………………………..11
1-4-4非勻相多分子膜(Heterogeneous multimolecular layers) ……………………………………………………………….12
1-5修飾電極的功能…..……………………………………………14
1-5-1電催化(electrocatalysis) ………………………………………14
1-5-2預濃縮(preconcentration) …………………………………….15
1-5-3薄膜阻隔(membrane barrier.) ..……………………………….16
1-6 Phenothiazine 修飾劑……………………………………….….18
1-6-1 Phenothiazine的基本性質……………………………..…….20
1-6-2 Phenothiazine衍生物在修飾電極上的應用………….……..20
1-7自動流注系統在修飾電極上的應………………………..……20
1-8本研究的目的………………………………………..……..…..22
Chapter 2
2-1藥品…………………………………………………………23
2-2儀器…………………………………………………………23
2-3電極的製備方法…………………………………………….24
Chapter 3
3-1 Phenothiazine對於苯胺偵測機制的探討…………………..25
3-2Phenothiazine修飾電極製備法的探討………………………28
3-3分析最佳化的探討……………………………………………29
3-4結論……………………………………………………………33

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