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研究生:陳臆如
研究生(外文):I-Ju Chen
論文名稱:人尿製劑對老年或去卵巢大鼠氧化應力相關指標的影響
論文名稱(外文):Effect of an urinary preparation on oxidative stress-related parameters in aged or ovariectomized rats.
指導教授:林文川林文川引用關係
指導教授(外文):Wen-Chuan Lin
學位類別:碩士
校院名稱:中國醫藥學院
系所名稱:中國藥學研究所
學門:醫藥衛生學門
學類:藥學學類
論文種類:學術論文
論文出版年:2000
畢業學年度:88
語文別:中文
論文頁數:104
中文關鍵詞:人尿製劑脂質過氧化超氧化物歧化脢穀胱甘太去卵巢大鼠骨質疏鬆cisplatin
外文關鍵詞:an urinary preparationlipid peroxidationsuperoxide dismutaseglutathioneovariectomized ratosteoporosiscisplatin
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在老化的過程中,因增齡而引起的中樞神經系統退化,已知部分原因與腦部產生活性氧而造成的細胞毒有關。所以有許多具有抗氧化作用的合成及天然物質被試著使用來預防因增齡而引起的中樞神經系統退化。我們研究室先前的研究發現人尿製劑具有很好的氫氧自由基清除作用。本實驗目的是使用老年雄性大鼠和去卵巢大鼠,來評估人尿製劑對老化的指標如如脂質過氧化反應、穀胱甘及超氧化物歧化的影響。另外,也探討人尿製劑對去卵巢大鼠引起的骨質疏鬆,及對cisplatin引起的腎毒性的影響。得到的結果如下:
1. 十五月齡的雄性大鼠,給予含人尿製劑 0.2 % 及1 %的飲水連續八個月。結果顯示,人尿製劑處理的大鼠,大腦皮質、血漿、主動脈、睪丸中的丙二醛濃度比控制組低。人尿製劑處理的大鼠,肝臟和腎臟的穀胱甘濃度顯著增加。另外,人尿製劑處理的大鼠,皮膚和腎臟氫脯氨酸含量明顯減少。
2. 去卵巢大鼠口服人尿製劑八週。不加鐵誘發丙二醛的濃度,在去卵巢大鼠組的大腦皮質、海馬迴、肝臟、心臟、子宮及腎上腺較偽手術組高,但在紋狀體二組無差異。人尿製劑處理的大鼠,對大腦皮質、海馬迴、紋狀體、腎上腺的不加鐵誘發丙二醛濃度,與控制組比較有減少作用。但對肝臟、心臟、子宮則無影響。加鐵誘發丙二醛的濃度,在去卵巢大鼠的大腦皮質、海馬迴、肝臟、心臟、子宮及腎上腺較偽手術組高。人尿製劑處理的大鼠,對海馬迴、紋狀體的加鐵誘發丙二醛濃度,與控制組比較有減少作用,但對大腦皮質、肝臟、心臟、子宮及腎上腺則無影響。在去卵巢大鼠的大腦皮質中,穀胱甘含量,超氧化物歧化、過氧化氫和穀胱甘過氧化的活性與偽手術組比較沒有差異,人尿製劑處理的大鼠,大腦皮質的穀胱甘過氧化活性下降,但對超氧化物歧化、過氧化氫的活性及穀胱甘的濃度沒有影響。
3. 大鼠分成偽手術組及去卵巢手術組,去卵巢大鼠放置十二週讓其形成骨質流失的狀態。在去卵巢手術後十二週,給予人尿製劑治療,於手術後二十週將大鼠犧牲。去卵巢大鼠和偽手術組比較有骨質疏鬆的情況,在股骨及第四腰椎的密度骨、灰份及鈣含量明顯下降。人尿製劑能降低去卵巢後所產生的影響。另外,人尿製劑對去卵巢大鼠的陰道及子宮有增重作用。這些結果顯示人尿製劑經由刺激雌激素接受體來改善去卵巢所誘發的骨質疏鬆。
4.大鼠先給予人尿製劑五天,第五天大鼠靜脈注射cisplatin ( 5mg/kg i.v.),而後再給人尿製劑三天。人尿製劑可以顯著的改善cisplatin所引的腎毒性。人尿製劑明顯改善cisplatin所引起的腎毒性,可從血清中血液尿素氮和肌酸肝明顯下降,以及明顯增加尿量和肌酸肝清除率看出。人尿製劑可增加經cisplatin排空的腎臟總均漿及粒線體層中的穀胱甘量。這些發現顯示,人尿製劑增加穀胱甘的含量,參與人尿製劑對cisplatin誘發腎毒性的保護作用。
   我們的研究結果顯示,人尿製劑可經由抗氧化作用,減緩老化的過程。本研究亦證實人尿製劑對於骨質疏鬆症是有益的。另外,人尿製劑可以對抗cisplatin所引起的腎毒性,顯示人尿製劑也許可以應用在cisplatin的化學療法。
During aging processes, it is believed that the age-related deterioration of the central nervous system is partly due to the cytotoxicity of reactive oxygen species generated in the brain. Thus, various synthetic or natural compounds having antioxidative properties have been tried to protect against the age-induced deterioration of CNS. In our previous works, we showed that a preparation of human urine (PHU) is a good scavenger of hydroxyl radical. The aim of this experiment was to evaluate the effects of PHU on the age-related parameters, such as lipid peroxidation (LPO), glutathione (GSH). and superoxide dismutase (SOD), in aged male rats and ovariectomized (OVX) rats. In addition, effect of PHU on the osteopenia in OVX rats, and nephrotoxicity induced by cisplatin were also studied. The following results were obtained.
1.The rats (15 months) were received PHU 0.2 or 1.0 g/dl drink water for 8 months. The results showed that MDA concentrations of brain cortex, plasma, aorta, testis were significantly lower in the PHU treated group as compared with the control group. The concentrations of GSH were significantly increased in the liver and kidney of rats treated with PHU. In addition, the diminution of hydroxyproline of skin and kidney in PHU treated rats were also observed.
2.The OVX rats were orally administered PHU for 8 weeks. Fe-independent MDA concentrations in brain cortex, hipocamppus, liver, heart, uterus and adrenal gland, but not in striatum, increased significantly in OVX controls compared to sham-operated rats. Fe-independent MDA concentrations in brain cortex, hipocamppus, striatum and adrenal gland, but not in liver, heart and uterus, decreased significantly in OVX rats treated with PHU compared to those in OVX rats. Fe-dependent MDA concentrations in brain cortex, hippocampus liver, heart and uterus, but not in striatum and adrenal gland, increased significantly in OVX controls compared to sham-operated rats. Fe-dependent MDA concentrations in striatum and hippocampus, but not in brain cortex, liver, heart, uterus and adrenal gland, decreased significantly in OVX rats treated with PHU compared to those in OVX controls. GSH contents and activities of SOD, catalase and GSH-Px of brain cortex were not significantly between the sham-operated group and OVX controls. However, PHU suppressed the activity of GSH-Px, but not SOD and catalase, in the brain cortex of OVX rats.
3.The rats were divided into the sham and OVX groups. The OVX rats were allowed to loss bone for 12 weeks. At 12 weeks post-OVX, the OVX rats were treated with PHU. They were killed 20 weeks postsurgery. OVX rats were osteopenic compared to sham by decreased bone density, ash and calcium content of femur and 4th lumbar spine. PHU decreasede these OVX-induced effects. In addition, PHU increased the weight of vargina and uterus in OVX rats. The data suggest that PHU ameliorated the osteopenia induced by OVX through stimulating the estrogen receptors.
4.Oral administration of PHU for 5 days before and 3 days after single injections of cisplatin (5 mg/kg, i.v. in rats) greatly ameliorated cisplatin-induced nephrotoxicity. PHU-induced amelioration of cisplatin nephrotoxicity was evident by significant reductions in serum BUN and creatinine, and significant improvement in urinary volume and creatinine clearance. PHU increased the GSH concentrations depleted by cisplatin in the renal totoal homogenates and mitochondrial fractions. The findings of this study suggest that increasing effect of PHU on the amounts of GSH be involved in the action of PHU on cisplatin-induced nephrotoxicity.
Our results suggest that PHU may delay the aging process by means of its antioxidant effects. This study also demonstrated that PHU is beneficial for osteopenia. In addition, PHU protects against cisplatin-induced renal toxicity, indicating that PHU might have applications for the use of cisplatin in cancer chemotherapy.
目錄
中文摘要…………………………………………………………………1
第一章 總論……………………………………………………………..4
第二章 人尿製劑對雄性老年大鼠的影響………………………….…11
第三章 人尿製劑對去卵巢大鼠的影響:抗氧化研究………………..36
第四章.人尿製劑對去卵巢大鼠的影響:骨質疏鬆研究………………51
第五章 人尿製劑拮抗cisplatin腎毒性作用………………………….64
結論……………………………………………………………………..76
參考文獻………………………………………………………………..77
英文摘要………………………………………………………………..90
附錄……………………………………………………………………..94
謝辭……………………………………………………………………104
表目錄
Table 2-1 Effect of long-term administration of PHU on body weight changes and mortality in the aged rats…………………….…..32
Table 2-2 Effect of long-term administration of PHU on the lipid peroxidation in various organs of the aged rats……………....33
Table 2-3 Effect of long-term administration of PHU on the contents of GSH in various organs in the aged rats………………………..34
Table 2-4 Effect of long-term administration of PHU on the contents of immune cells in the blood. in the aged rats…………………..35
Table 3-1 Effect of PHU on the activity of SOD in the three region of the brain in the ovariectomized rats………………………………49
Table 3-2 Effect of PHU on the Fe+2-independent and Fe+2-dependent lipid peroxidation in heart , liver , uterus , adrenal gland in ovariectomized rats…………………………………………...50
Table 4-1 Effect of PHU on the serum concentrations of estradiol , calcium and amount of urinary calcium.……………………...63
Table 5-1 Effect of PHU on the activities of SOD ,Catalase ,GSH-Px of cisplatin-treated rats…………………………………………..75
圖目錄
Fig. 2-1 Effect of long-term adminstration of PHU on lipid peroxidation in three regions of the brain in the aged rats…………………..28
Fig. 2-2 Effect of long-term administration of PHU on the activity of SOD in three regions of the brain in the aged rats………………………………………………………………29
Fig. 2-3 Effect of long-term administration of PHU on the contents of hydroxyproline in skin (A) and kidney (B) in the aged rats…..30
Fig. 2-4 Effect of long-term administration of PHU on the concentrations of testosterone in the serum in the aged rats……………….…..31
Fig. 3-1 Effect of PHU on the Fe+2-independent and Fe+2-dependent lipid peroxidation in three regions of the brain in the ovariectomized rats………………………………………………………….…..47
Fig. 3-2 Effect of PHU on the content of GSH , and the activities of GSH-Px, catalase in the brain cortex of the ovariectomized rats….…48
Fig. 4-1 Effect of PHU on the changes of body weight in ovariectomized rats……………………………………………………….……...57
Fig. 4-2 Effect of PHU on the concentration of cholesterol in ovariectomized rats……………………………………………..58
Fig.4-3 Effect of PHU on the weight of pituitary(A) , uterus(B) and vagina(c) in ovariectomized rats……………………………...59
Fig. 4-4 Effect of PHU on the bone density of 4th lumbar vertebra and right femur in the ovariectomized rats………………………….60
Fig. 4-5 Effect of PHU on the bone ash of 4th lumbar vertebra and right femur in the ovariectomized rats…………………………….…61
Fig. 4-6 Effect of PHU on the bone calcium of 4th lumbar vertebra and right femur in the ovariectomized rats………………………..62
Fig. 5-1 Protective effect of PHU against cisplatin -induced elevation of serum creatinine and blood urea nitrogen (BUN ) in rats…….71
Fig. 5-2 Protective effect of PHU against cisplatin -induced changes in urine volume and creatinine clearance in rats………………...72
Fig. 5-3 Effect of PHU on the lipid peroxidation in the renal total homogenate (A) and mitochondrial fraction (B) of cisplatin -treated rats…………………………………………………….73
Fig. 5-4 Effect of PHU on the contents of GSH in the renal total homogenate (A) and mitochondrial fraction (B) of cisplatin -treated rats……………………………………………………74
附錄
Table 1 Absolute organ weights in aged rats treated with PHU for 8 months………………………………………………………...94
Table 2 Relative organ weights in aged rats treated with PHU for 8 months………………………………………………………95
Table 3 Hematological findings in aged rats treated with PHU for 8 months…………………………………………………...96
Table 4 Blood chemistry findings in aged rats treated with PHU for 8 months………………………………………………………97
Table 5 Urinary findings in aged rats treated with PHU for 8 months…98
Table 6 Absolute organ weights in ovarectomized rats treated with PHU for 8 weeks……………………………………………………99
Table 7 Relative organ weights in ovariectomized rats treated with PHU for 8 weeks……………………………………………………100
Table 8 Urinary findings in ovariectomized rats treated with PHU for 8 weeks……………………………………………………….101
Table 9 Blood chemistry findings in ovariectomized rats treated with PHU for 8 months……………………………………………102
Table 10 Hematological findings in ovariectomized rats treated with PHU for 8 months……………………………………………103
1. 中藥大辭典,上海科學技術出版社,上海,pp.27-29,1986。
2. 吳繼星,尿淘金,科技信息,3:32-40,1990。
3. 唐高霞、竇鴻斌,人尿的利用,今日科技2:31-32,1989。
4. 宋原智,尿的開發與綜合利用,中國生化藥物雜誌1:71-74,1992。
5. 蔣秉植、楊健美,人尿中的活性物質的開發,化工進展5:39-42,1993。
6. (明)李時珍,本草綱目,國立中國醫藥研究所,台北,p.1606,1988。
7. 中藥大辭典,上海科學技術出版社,上海,p.36,1986。
8.賴東淵:人尿製劑抗氧化及保肝作用之研究,中國藥學研究所博士論文,台中1998。
9.廖媛璋:人尿製劑對大鼠初代肝細胞之麩胱甘及其相關酵素之影響,中國藥學研究所碩士論文,台中1998。
10.(明)李時珍,本草綱目,國立中國醫藥研究所,台北,pp.1604-1606,1988。
Abrass, C.K., Adcox, M.J. and Raugi, G.J. (1995) Aging-associated changes in renal extracellular martix. Am. J. Pathol. 146, 742-752.
Aebi, H.(1984) Catalase in vitro. Methods in Enzymol. 250,121-126.
Aejmelaeus, R.T., Holm, P., Kaukinen, U., Metsa-Ketela, T.J.A., Laippala, P., Hervonen, A.L.J. and Alho, H.E.R. (1997) Age-related changes in the peroxyl radical scavenging capacity of human plasma. Free Radic. Biol. Med. 23, 69-75.
Al-Harbi, M.M., Osman, A.M.M., Al-Bekairi, A.M., Al-Shababah, O.A., Sabah, D.M. and Raza, M. (1995) Effects of desferrioxamine on cisplatin-induced nephrotoxicity in normal rats. Chemotherapy 41, 448-454.
Allen R.G.(1993) Free radicals and differentiation : the interrelationship of development and aging .In:Yu . B. P., (ed.) Free in aging ,Boca Raton , FL:CPC Press .12-23.
Aust, S.D., Morehouse, L.A. and Thomas, C.E. (1985) Oxygen toxicity, oxygenradicals, transition metals and disease. Free Radi. Biol. Med. 1, 3-25.
Azhar, S., Cao, L. and Reaven, E. (1995) Alteration of the adrenal antioxidant defense dydtem during aging in rats. J. Clin. Inves. 96, 1414-1424.
Benzi, G and Moretti, A. (1995) Age and peroxidative stress-related modifications of the cerebral enzymatic activities linked to mitochondria and the glutathione system. Free Radic. Biol. Med. 19, 77-101.
Benzi, G., Pastories, O., Marzatico, F. and Villa, R.F. (1988) Influence of aging and drug treatment on the cerebral glutathione system. Neurobiol. Aging 9, 371-375.
Beutler, E., Duron, O.,and Kellyu, BM.(1963)Improved method for the determination of blood glutathione . J Lab & Clin Med. 61,882-888.
Birge, S.J. (1998) Hormones and the aging brain. Geriatrics 53, S28-S30.
Braughler, J.M., Pregenzer, J.F., Chase, R.L., Duncan, L.A., Jacobsen, E.J. and McCall, J.M. (1987) Novel 21-amino steroid as potent inhibitors of iron-dependent lipid peroxidation. J. Biol. Chem. 262, 10438-10440.
Bromont, C., Marie, C. and Bralet, J. (1989) Increased lipid peroxidationin vulnerable brain regions after transient forebrain ischemia in rats. Stroke 20, 918-924.
Burns,EA.,and Goodwin,JS.,(1997) Immunodeficiency of aging .Drug & Aging. 11(5): 374-97.
Burnett, CC, Reddi AH (1983) Influence of estrogen and progesterone on matrix-induced endochondral bone formation.Calcif Tissue Int.35.609-614.
Burzynski, S.R. (1988) Isolation, purification, and synthesis of antineoplastons. In : Advances in experimental and clinical chemotherapy: antineoplaston II (ed.) : Kuemmerle, H.P. p.1-8, Burzynski Research Institute, Inc. Houston.
Cand, F. and Verdetti, J. (1989) Superoxide dismutase, glutathione peroxidase, catalase, and lipid peroxidation in the major organs of the aging rats. Free Radic. Biol. Med. 7, 59-63.
Carter,N.P.,(1990) Measurement of cellular subsets using antibodies.In: Ormerod MG,ed.Flow cytometry : A practical approach .Oxford.:ILR Press,45-67.
Coudary, C., Rouseel, A.M., Arnaud, J. and Favier, A. (1997) Selenium and antioxidant vitamine and lipid peroxidation levels in preaging French population. EVA study group. Edude de vieillissement arteriel. Biol Trace Elem Res. 57, 183-190.
Danh, H.C., Benedetti, M.S. and Destert, P. (1983) Differential changes in superoxide dismutase activity in brain and liver of old rats and mice. J. Neurochem. 40, 1003-1007.
De Vries, J. (1996) Cytoxicity: molecular mechanisms of cell death. In : Toxicology : Principle and applications. Niesink RJM, De Vries, J, Hollinger, M.A., (ed.) CPC Press, New York, pp.289-313.
De Zwart, L.L., Merrman, J.H.N., Commandeur, J.N.M. and Vermeulens, N.P.O. (1999) Biomarkers of free radical damage applications in experimental animals and in human. Free. Radic. Biol. Med. 26, 202-226.
De, A.K. and Darad, P. (1991) Age-associated changes in antioxidants and antioxidative enzymes in rats. Mecam. Ageing Dev. 59, 123-128.
Dogru-Abbasoglu, S., Tamer-Toptani, S., Ugurnal, B., Kocak-Toker, N., Aykac-Toker, G. and Uysal, M. (1997) Lipid peroxidation and antioxidant enzymes in livers and brains of aged rats. Mech. Ageing Dev. 62, 111-116.
Evans, P.H. (1993) Free radicals in brain metabolism and pathology. Bri. Med. Bull. 49, 577-587.
Farooqui, MY, Day,WW.,and Zamorano,DM., (1987) Glutathione and lipid peroxidation in the aging rat.Comp.Biochem.Physiol..88,177-180.
Fletcher,R.H.,and Fletcher,S.W., (1994) Glutathione and ageing : ideas and evidence. The Lancet.344,1379-1380.
Garrido, A., Garate, M., and Valenzulea, A. (1993) Changes in the antioxidant capacity of blood plasma are produced after Res comm Chem Pathol Pharmacol. 367-370.
Glowinski,J.,and Iversen,L.L.,(1966) Regional studies of catecholamines in the rat brain I: The disposition of [3H]norepinephrine , [3H]dopamine and [3H]dopa in various regions of the brain.J.Neurochem.33,655-69.
Gibbs, R.B. and Aggarwal, P. (1998) Estrogen and basal forebrain cholinergic neurons: implications for brain aging and Alzheimer''s diseae-related cognitive decline. Horm and Behav 34, 98-111.
Griffith, O.W. (1999) Biological and pharmacologic regulation of mammalian glutathione synthesis. Free Radic. Bbiol. Med. 27, 922-935.
Hanfeman, DG, Sunde, RA, and Hoekstra, WG.(1974) Effect of dietary selenium on erythrocyte and liver glutathione peroxidase in the rats. J Nutr .104, 580-587.
Harrison, E., Adjei, A., Amecho C., Yamamoto S.,Kono S.(1998) The effect of soybean protein on bone loss in a rat model of postmenopusal osteoporosis.J. Nutri Sci. Vita.44(2).257-68.
Harman, D.(1968) Aging: A theory based on free radical and radiation chemistry.J.Gerontol. 11, 298-300.
Hua Zhu K.E., Hollis A., Simmons, Christine M.,Pirie D.,Todd crawford, and David D., Thompson.(1995) Droloxifene , a new estrogen antagonist/agonist,prevents bone loss in ovariectomized rats.The Endocrine Soci.136 (6 ).2435-2441.
Janero, D.R. (1990) Malondialdehyde and thiobarbituric acid-reactivity as diagonistic indices of lipid peroxidation and peroxidative tissue injury. Free Radic. Biol. Med. 9, 515-540.
Juricova, M. and Deyl, Z. (1975) Ageing processes in collagens from different tissues of rats. Adv. Exp. Med. Biol. 53, 351-357.
Kalu, D.N.,Hardin,RR,Cockerhan, R.(1984) Evaluation of the pathogenesis of skeletal change in ovariectomized rats. Endocrinology.115,507-512.
Kalu, D.N. , Hardin, RR,(1984) Evaluation of the role of calcitonin deficiency in ovariectomized-induce osteopenia. Life Sci.34 ,2394-2397.
Kalu, D.N., Liu, C.C., Salerno, E., Hollis, B., Echon, R. and Ray, M. (1991).Skeletal response of ovariectomlized rats to low and high does of 17b-estradiol.Bone and Miner.14,175-187.
Kameyama, Y. and Gemba, M. (1991) Cisplatin-induced injury to calcium uptake by mitochondria in glutathione-depleted slices of rat kidney cortex. Japan. J. Pharmacol. 55, 74-76.
Kawai, M., Matsuura, S., Asanuma, M. and Ogawa, N. (1998) Manda, a fermented natural food, suppress lipid peroxidation in the senescent rat brain. Neurochem. Res. 23, 455-461.
Kimuya, Y., Kubo, M., Tani, T., Arichi, S., Okuda, H.(1981) Studies on scutellarie Radix. IV. Effects on lipid peroxidation in ret liver. Chem & Pharm Bull. 29(9) 2618-23.
Knight, J.A. (1995) The process and theories of aging. Ann. Clin. Lab. Sci. 25, 1-12.
Kuhlmann, M.K., Burkhardt, G. and Kohler, H. (1997) Insights into potential cellular mechanisms of cisplatin nephrotoxicity and their clinical application. Nephrol Dial Tranplant 12, 2478-2480.
Lai, T.Y.,Wu Y.W. and Lin W.C.(1999)Effect of a urinary preparation on liver injury by short-term carbon tetrachloride treatment in rats.Am. J.Chin. Med.2,241-249.
Leibbrandt, M.E., Grushenka, H.I.W., Metz, A.L., Ozbia, A.A. and Haskins, J.R. (1995) Critical subcellular targets of cisplatin and related platinum analogs in rat renal proximal tubule cells. Kidney Int. 48(3) , 761-770
LeMaoult, J., Szabo, P., Weksler, M.E. (1997) Effect of age on humoral immunity ,selection of the B-cell repertorie and B-cell development. Immunol. Rev.,160, 15-126.
Levi,J., Jacobs,C., Kalman,SM., McTigue, M., and Weiner,MW.,(1980) Mechanism of cis-platinum nephrotoxicity :I.Effects of sulfhydryl group in rat kidneys.J.Pharmacol Exp Ther .213,545-550.
Li, X.,Takahashi,M.,Kushida,K.,and Inoue,T., (1998) The preventive and interventional effects of raloxifene analog (LY117018 HCL) on osteopenia in ovariectomized rats.13(6),1005-1010.
Liau, M.C.,Lee, S.S., and Burzyski, S.R.(1988) Differentiation inducing components of antineoplaston A5 Adrv. Exptl .Clin Chemother. 88, 9-25.
Lin, W.C., Wu, Y.W. and Lai, T.T.(2000) Scavenging of reactive oxygen species by an urinary preparation.Am. J. Chin. Med.(inpress)
Liu,C.C.,Kalu, D.N., (1990) Preexisting bone loss associated with ovariectony is reversed by parathyroid hormone.J Bone Min Res. 5:239.
Liu,J.and Mori,A.,(1993) Age -associated changes in superoxide dismutase activity, thiobarbituric acid reactivity and reduced glutathione level in the brain and liver in senescence accelerated mice (SAM) : a comparison with ddY mice. Mech.Ageing Dev. 71,23-30.
Lovell, M.A., Ehmann, W.D., Butler, S.M. and Markesbery, W.R. (1995) Elevated thiobarbituric acid-reactive substance and antioxidant enzyme activity in the brain in Alzheimer''s disease. Neurology 45, 1594-1601.
Lowry, O.H. , Rosebrough, N.J. , Farr, A.L. , and Randall, R.J.(1951) Protein measurment with the folinophenol reagent. J. Biol. Chem.193,265-275.
Luo, S. Aourla,A. Larrie,C. Gauthier,S. Merand,Y. Belanger,A. and Labrie,F. (1998) Effect of twenty-four-week treatment with the antiestrogen EM-800 on estrogen-sensitive parameters in intact and ovariectomized mice.Endocrinology.139 (5),2645-2656.
Mackall, C.L., Punt, J.A., Morgan, P., Farr, A.G. and Gress, R.E. (1998)Thymic function in young/old chimeras: substantial thymic T cell regenerative capacity despite irreversible age-associated thymic involution. Eur. J. Immunol. 28, 1886-1893..
Markesbery,WR.,(1997) Oxidative stress hypothesis in Alzheimer''s disease. Free Radic.Biol.Med.23(1):134-47.
Markesbery,WR.,and Carney,JM.,(1999) Symposium: Oxidative stress in neurological disease-oxidative alterations in Alzheimer''s disease.Brain Pathology.9,133-146.
Marklund, S.and Marklund, G.(1975).Involvement of superoxide anion radical in the autoxication of dyrogallol and convenient assay for superoxide dismutase. Eur J. BioChem. 47, 469-474.
Matsuo, M., Gomi, F. and Dooley, M.M. (1992) Age-related alterations in antioxidant capacity and lipid peroxidation in brain, liver, and lung homogenates of normal and vitamin E-deficient rats. Mech. Ageing Dev. 64, 273-292.
Matsushima, H., Yonemura, K., Ohishi, K. and Hishida, A. (1998) The role of oxygen free radicals in cisplatin-induced acute renal failure in rats. J. Lab. Clin. Med. 131, 518-526.
Mayes, P.A. (1988) Nutrition, digestion, and absorption. In: Harper''s Biochemistry. Murray, R.K., Granner, D.K., Mayes, P.A. and Rodwell, V.W. (ed.). Prentice-Hall International Inc. 23 edition, pp. 571-588.
Mcintosh, L.J., Trush, M.A. and Tronocoso, J.C. (1997) Increased susceptibility of Alzheimer''s disease temporal cortex to oxygen free radical-mediated processes. Free Radic. Biol. Med. 23, 183-190.
Mizuno, Y. and Ohta, K. (1986) Regional distributions of thiobarbituric acid-reactive products. Activities of enzymes regulating the metabolism of oxygen free radicals, and some of the related enzymes in adult and aged rats brains.J. Neurochem. 46, 1344-1352.
Neuman, R.E. and Logan, M.A.(1950) The determination of hydroxyproline. J. Biol. Chem .184, 299-306.
Ohkawa, H., Ohishi, N. and Yagi, K.(1979) Assay for lipid peroxides in animal tissue by thiobarbituric acid reaction. Anal.Biochem.95,351-358.
Ohtsuki, T. Matsumoton, M , Suzuk, Taniguchi, N., and Kamada, T.(1995) Mitochondrial lipid peroidation and superoxide dismutase in rat hypertensive target organs. Am. J. Physiol.268,H1418-H1421.
Pacifici, R.E. and Davies, K.J.A. (1991) Protein, lipid and DNA repair system in oxidative stress: the free-radical theory of aging revisited. Gerontol. 37, 166-180.
Perry, H.M. (1999) The endocrinology of aging. Clin. Chem. 45, 1369-1376.
Persky, A.M., Green, P.S., Stubley, L., Howell, C.O., Zaulyanov, L., Brazeau, G.A. and Simpkins, J.W. (2000) Protective effect of estrogen against oxidative damage to heart and skeletal muscle in vivo and in vitro. Proc. Soc. Exp. Biol. Med. 223, 59-66.
Provinciali, M., Di Stefano, G., Bulian, D., Stronati, S. and Fabris, N. (1997) Long-Pushpendran, C.K., Subramanian, M., Devasagayam, T.P.A. and Singh, B.B. (1998) Study on lipid peroxidation potential in different tissue induced by ascorbate- Fe+2: Possible factors involved intheir differential susceptibility. Mol. Cell. Biochem. 178, 197-202.
Pushpendran,C.K.,Subramanian,M.,Devasagayam,T.P.A., and Singh,B.B.,
(1998) Study on lipid peroxidation potential tissue induced by ascorbate-Fe+2 possible factors involved in their different susceptibility. Mol.Cell Biochem.178,197-202.
Ravindranath,V., Shivakumar,B.R.,and Anandatheerthavarada,H.K.,(1989) Low glutathione levels in brain regions of aged rats. Neurosci. Lett.101,187-190.
Ramassamy, C., Averill, D., Beggert, V. Bastiantetto, S.,Theroux, L.,Lussier-Cacan, S.,Cohn, J.S.,Christen, Y.Davignen, J. Quirion, R. and Poirier, J.(1999)Oxidative damage and protection by Antioxidants in the frontal cortex of Alzheimer''s disease is related to the Apolipoprotein E, Genotype. Free Radic Biol Med. 27 , 544-553.
Reddel, R.R. (1998) A reassessment of the telomere of senescence. Bioessays 20, 977-84.
Reiser, K.M. (1998) Nonenzymatic glycation of collagen in aging and diabetes. Proc. Soc. Exp. Biol. Med. 218, 23-37.
Sadzuka, Y., Shoji, T. and Takino, Y. (1992) Mechanism of the increase in lipid peroxide induced by cisplatin in the kidneys of rats. Toxicol. Lett. 62, 293-300.
Safirstein, R., Miller, P. and Guttemplan, J.B. (1984) Uptake and metabolism of cisplatin by rat kidney. Kidney Int. 25, 753-759.
Sahoo, A. and Chainy, G.B.N. (1997) Alterations in the activities of cerebral antioxidnt enzymes of rat are related to aging. Int. J. Neurosci. 15, 939-948.
Santiago, L.A., Osato, J.A., Liu, J. and Mori, A. (1993) Age-related increases in superoxide dismutase activity and thiobarbituric acid-reactive substances: effect of bio-catalyzer in aged rat brain. Neurochem. Res. 18, 711-717.
Saoth, K. (1978) Serum lipid peroxides in cerebrovascular disorders determined by a new caloremetric methods. Clin. Chim. Acta. 90, 370-43.
Sawada, M. and Carlson, J.C. (1987) Changes in superoxide radical and lipid peroxide formation in the brain, heart and liver during the lifetime of the rat. Mech. Aging Dev. 41, 125-137.
Sedlak , J.and Lindsay,R.H.(1968) Estimation of total, protein-bound , and nonprotein sulfhydryl group in tissue with Ellman''s reagent .Anal. Biochem. 25,192-205.
Sell, D.R. (1997) Ageing promotes the increase of early glycation Amadori product as assessed by epsilon-N-(2-furoylmethyl)-L-lysine (furosine) level in rodent skin collage. The relationship to dietary restriction and glycoxidation.
Sies, H. (1999) Glutathione and its role in cellular functions. Free Radic. Biol. Med. 27, 916-921.
Smith, M.A., Harris, P.L.R., Sayre, L.M., Perry, G. (1997) Iron accumulation in Alzhemer''s disease is asource of redox-generated free radicals. Proc. Natl. Acad. Sci. USA 94, 9866-9868.
Stocks, J., Gutteridge, J.M.C., Sharp, R.J. and Dormandy, T.L. (1974) Assay using brain homogenate for measuring the antioxidant activity of biological fluids. Clin. Sci. Molec. Med. 47, 215-222.
Thibault, A., Samid, D. and Cooper, MR.(1995) Phassse I study of phenylacetate administered twice daily to patients with cancer. Cancer. 75,2932-2938.
Tian, L. Cai, Q and Wei, H. (1998) Alterations of antioxidant enzymes and oxidative damage to macromolecules in different organs of rats during aging. Free Radic. Biol. Med. 24, 1477-1484.
Toran-Alleerand, C.D., Singh, M. and Setalo, G.J. (1999) Novel mechanisms of estrogen action in the brain: new players in an old story. Frontiers in Neuroendocrinol. 20, 97-121.
Turner RT, Vanderstcenhoven, JJ, Bell, NH.(1987) The effect of ovariectomy and 17b-estadiol on cortical bone histomophometry in growing rats.J. Bone Min Res.2.115-122.
Umeki, S., Sumi, M., Niki, Y. and Soejima, R. (1987) Concentrations of superoxide dismutase and superoxide anion in blood of patients with respiratory infections and compromised immune system. Clinical Chemisty. 33(12),2230-3.
Van Nesselrooij,J.H.,Szeverenyi,N.M.,and Ruocco,M.J., (1989) Magnetic resonance imaging of estrogen-induced pituitary hypertrophy in rats.Magnetic Resonance in Medicine.11,161-171.
Vermeulen, N.P.E. and Baldew, G.S. (1992) The role of lipid peroxidation in the nephrotoxicity of cisplatin. Biochem. Pharmacol. 44, 1193-1199.
Wamer, M., Nilsson, S. and Gustafsson, J.A. (1999) The estrogen receptor family. The Opinion in Obstetrics and Gyneology 11, 249-254.
Wang, Q., Santizo, R., Baughman, V.L., Pelligrino, D.A., and Iadecola, C. (1999) Estrogen provides neuroprotection in transient forebrain ischemia through perfusion-independent mechanisms in rats. Stroke 30, 630-637.
Wiseman, H. (1995) Dietary influence on membrane function: importance in protection against oxidative damage and disease. J. Nutr. Biochem. 7, 2-15.
Wronski,T.J, Cintron M, Doherty AL,Dann LM. (1988) Estrogen treatment prevents osteopenia and depress bone turnover in ovariectomized rats.Endovrinology.123,681-686.
Yagi, K. (1997) Female hormones act as natural antioxidants? a survey of our research. Acta Biochimica Polnica 44, 701-710.
Zhang, J.G. and Lindup, W.E. (1994) Cisplatin nephrotoxicity: decrease in mitochondrial protein sulphydryl concentration and calcium uptake by mitochondria from rat renal cortical slices. Biochem. Pharmacol. 47, 1127-1135.
Zhang, J.R., Andrus, P.K. and Hall, E.D. (1993) Age-related reginoal changes in hydroxyl radical stress and antioxidants in gerbil brain. J Neurochem. 61, 1640-1647.
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