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研究生:許麗秋
研究生(外文):Li Chiou Hsu
論文名稱:尿中BTEX與其代謝產物關係之研究
論文名稱(外文):Study on the Relationships between BTEX in Urine and its metabolites
指導教授:郭錦堂郭錦堂引用關係
指導教授(外文):C-T Kuo
學位類別:碩士
校院名稱:中國醫藥學院
系所名稱:環境醫學研究所
學門:醫藥衛生學門
學類:公共衛生學類
論文種類:學術論文
論文出版年:2000
畢業學年度:88
語文別:中文
論文頁數:71
中文關鍵詞:甲苯乙苯二甲苯生物偵測固相微萃取頂空法
外文關鍵詞:benzenetolueneethylbenzenexylenebiological monitoringsolid-phase microextractionheadspace
相關次數:
  • 被引用被引用:10
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  • 下載下載:46
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摘 要
對於環境中有害物濃度之監測,一般以採集空氣樣本來測定其暴露情況,如此只能提供單一暴露途徑的潛在危害,為了能同時瞭解有害物進入人體劑量的多寡,本研究不僅掌握空氣中有害物濃度,同時以較不具侵略性與便利性之尿液樣品作為生物偵測方法,以高效率液相層析儀進行BTEX多個標的物質代謝產物測定,並利用一種靈敏度高、使用方便、偵測極限可達pg之萃取技術?固相微萃取法(solid-phase microextraction,簡稱SPME),測定尿中BTEX未代謝原形物質濃度。本研究選定中部某彩色印刷工廠勞工為對象,進行環境測定及生物偵測,以探討空氣中BTEX濃度與尿中代謝產物、尿中未代謝原形物質三者間的相關性。
本研究利用固相微萃取法經由測試評估後,其檢量線線性相關係數均高於0.999,偵測極限可達pg、再現性測試之變異係數均小於13.62%,尿液樣品貯存於4℃時,其穩定性仍能維持30天。此技術應用於暴露BTEX作業勞工尿液之生物偵測確實為可行、便利之測定方法。另外,實際測定職業暴露者之結果顯示,空氣中BTEX濃度之分佈在0.16 ~ 4.29 ppm之間,其中以乙苯之濃度較其他物質為高;尿中BTEX代謝產物分析結果,各代謝產物濃度均低於ACGIH所規定之BEIs值,尿中未代謝原形物質之測定以乙苯濃度較高,三者經由統計相關性分析後,空氣中苯與尿中代謝產物t,t-MA相關性達0.86,但無達統計上顯著意義,乙苯與尿中代謝產物MA相關為0.5 6(經creatinine校正,p<0.05)及0.57 (經比重校正,p<0.05),(間+對)-二甲苯與尿中代謝產物(m+p)-MHA之相關性為0.77(經creatinine校正,p<0.01)及0.56(經比重校正,p<0.05),尿中BTEX未代謝原形物質中僅有乙苯與空氣中乙苯相關較佳(r=0.60,p<0.05),其他則沒有良好相關性。
Abstract
The major method to determine the toxical benzene, toluene, ethylbenzene and xylenes(BTEX) in the environment was performed by adapting air as sample directly and measuring its volume. It only could tell us the concentration of BTEX in the environment, but we had no idea about the concentration of BTEX in our human bodies. In this study, a more convenient and less invasive method was used; it could determine the concentration of BTEX both in the environment and in human urine. Solid-phase microextraction(SPME) has been used for sampling BTEX from the headspace(HS) of urine and gas chromatography(GC) has been applied for analysis. The urine samples were from a print factory in middle-Taiwan, and the relationships for concentration of BTEX among the environmental air, urinary metabolites and urinary unmetabolites were inspected.
The HS-SPME-GC method showed a very high relative linear correlation of 0.999 and the limit of detection was to pg. The coefficient of variances of reproducibility was less than 13.62%. Urine samples were stable for 30-day storage at 4℃. It is a practicable and convenient method for biological monitoring of BTEX, which the workers were exposed to. In addition, it revealed that the concentration of BTEX in air was 0.16~4.29ppm, and that of ethylbenzene was the highest among them; the concentrations of BTEX in urinary metabolites were lower than BEIs value regulated by ACGIH; the concentration of ethylbenzene in urinary unmetabolites was the highest among BTEX.
After statistical analysis of correlation, it showed that the correlation of concentration between environmental air and t,t-MA in urinary metabolites was 0.86 and had no significant difference; the correlation of concentration between ethylbenzene in environmental air and MA in urinary metabolites were 0.56(corrected with creatinine, p<0.05)and 0.57(corrected by specific gravity, p<0.05), respectively; the correlation of concentration between (m+p)-xylene in environmental air and (m+p)-MHA in urinary metabolites were 0.77(corrected with creatinine, p<0.01) and 0.56(corrected by specific gravity, p<0.05), respectively; the correlation of concentraction between ethylbenzene in environmental air and ethylbenzene in urinary unmetabollites was 0.60(p<0.05).
目 錄
中文摘要……………………………………………………………..I
英文摘要……………………………………………………………..II
目錄…………………………………………………………………..III
表目錄………………………………………………………………..V
圖目錄………………………………………………………………..VI
壹、前言
一、研究動機……………………………………………………..1
二、研究目的……………………………………………………..3
貳、文獻回顧
一、BTEX化合物之性質、用途及危害…………………………4
二、BTEX化合物之代謝產物……………………………………6
三、尿中代謝產物之校正方法…………………………………..8
四、尿中BTEX未代謝原形物質測定之相關研究……………..9
五、固相微萃取技術之介紹與應用……………………………..12
六、固相微萃取法之優點………………………………………..13
參、材料與研究方法
一、材料…………………………………………………………..14
(一)藥品………………………………………………………...14
(二)實驗器具…………………………………………………...15
(三)儀器設備…………………………………………………...16
二、實驗方法……………………………………………………..17
(一)空氣中BTEX採樣及分析方法……………………………17
(二)尿液樣品採集與分析方法………………………………...18
A)尿中BTEX代謝產物分析………………………………..19
B)尿中BTEX未代謝原形物質分析………………………..21
(三)資料處理與分析…………………………………………...26
肆、結果
一、作業環境測定分析結果……………………………………..27
二、尿液樣品之生物偵測………………………………………..28
(一)尿中BTEX代謝產物之分析………………………………28
A.分析方法之建立…………………………………………..28
B.尿中BTEX代謝產物之分析品質管制…………………..29
C.職業暴露勞工尿中BTEX代謝產物之分析……………..30
(二)尿中BTEX未代謝原形物質之分析………………………30
A.最適分析條件之建立……………………………………..30
B.尿中BTEX未代謝原形物質之分析品質管制…………..33
C.職業暴露勞工尿中BTEX未代謝原形物質之分析……..35
三、空氣中BTEX濃度與尿中代謝產物、未代謝原形物質相關性探討……35
伍、結論……………………………………………………………..38
陸、討論與建議……………………………………………………..40
參考文獻……………………………………………………………..41
參考文獻
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