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研究生:陳晉勳
研究生(外文):Chin-Hsun Chen
論文名稱:切除卵巢之大白鼠其血中leptin含量的變化
論文名稱(外文):Response of serum leptin level in rats after ovariectomy and Estrogen supplement
指導教授:周芬碧周芬碧引用關係
指導教授(外文):Fen-Pi Chou
學位類別:碩士
校院名稱:中山醫學院
系所名稱:生物化學研究所
學門:生命科學學門
學類:生物化學學類
論文種類:學術論文
論文出版年:2000
畢業學年度:88
語文別:中文
論文頁數:80
中文關鍵詞:雌性素瘦身蛋白卵巢切除術
外文關鍵詞:estrogenleptinoverietomy
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本實驗主要是探討停經前及停經後的婦女( postmenopausal ),體重增加的機制,在這個研究中,使用卵巢切除來誘導肥胖的大白鼠模式,來探討leptin在這過程中所扮演的角色。在這實驗過程中,進行長達17週長時間的監控紀錄大白鼠在失去雌性素(estrogen)之後,血清中平均leptin的變化,同時觀察給予雌性素對於leptin的影響。
結果顯示,除去卵巢那組(OVX)平均的體重在三星期後已經遠高於其它兩組老鼠的平均體重,並且持續的增加直到實驗終止。OVX組隨著重量的增加,同時也扮隨著血中平均leptin濃度的改變,到了實驗一半期間第7週時,OVX組大部分的老鼠血清中平均leptin的濃度幾乎無法被偵測到(0.216±0.132ng/ml),隨後它的濃度開始升高,到了實驗結束終了,leptin平均的濃度測得為(3.182±0.936ng/ml),很明顯地比手術前所測得leptin平均的濃度(0.818±0.242ng/ml)大約高於四倍左右,這些血清leptin數值的上下波動應造因於卵巢的切除,而雌性素的補充則會使此現象消失。
本論文的証據顯示卵巢切除平均誘導體重的增加,可能是因為早期平均leptin濃度的降低所造成的,而最後平均leptin的升高和平均體重的增加,很可能與leptin訊息的敏感度的下降有所關連。
This experiment is aimed at discussing the mechanism of gaining weight of women between “ Postmenopausal ײ from “ Primenopausal ײ During the research, using “ Ovariectomy — induced obesity model ײ to observing how leptin plays a role during the procedure.
During a 17-week-long experiment, we observed the variation and influence of the leptin when the “ Estrogen ײ is loses and “ Estrogen ײ gain of in rats. According to the results, the weight gaining on the OVX group, which “Ovariectomy-induced obesity model ײ was applied, was far ahead than the other two groups in their average weight in three weeks and continued increasing until the last week. The leptin level in blood was increased with the weight gain of the OVX group. However, the above leptin level, whick was at the unit of (0.216±0.132ng/ml), was hardly detected when at mid-term of the 7 th week. After this, its leptin level started gaining to the unit of (3.182±0.936ng/ml) until the final procedure of the experiment. It is obvious that the level of the leptin was around four times than it was before the operation (0.818±0.242ng/ml). The flutuation of leptin’s level were caused by the “Ovariectomy” induces the increase of weight, which is caused by the leptin level decreased in the early stage. However, the final increasing of leptin level and its weight gain may be influnced by the sensitivity of the leptin signal.
一、英文摘要1
二、中文摘要2
三、緒言3
四、研究動機16
五、實驗材料與方法17
六、實驗結果27
七、討論29
八、圖表與圖表說明34
九、參考文獻40
十、附錄52
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