跳到主要內容

臺灣博碩士論文加值系統

(44.211.26.178) 您好!臺灣時間:2024/06/15 01:44
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

: 
twitterline
研究生:林長安
研究生(外文):Chang-An Lin
論文名稱:不同運動量後抗氧化活性及訊息傳遞蛋白之表現
論文名稱(外文):Effect of different exercise intensity on the activities of antioxidant enzymes and expression signal transduction proteins
指導教授:王朝鐘王朝鐘引用關係
指導教授(外文):Chau-Jong Wang Ph.D
學位類別:碩士
校院名稱:中山醫學院
系所名稱:生物化學研究所
學門:生命科學學門
學類:生物化學學類
論文種類:學術論文
論文出版年:2000
畢業學年度:88
語文別:中文
論文頁數:94
中文關鍵詞:一氧化氮
外文關鍵詞:Nitric oxide
相關次數:
  • 被引用被引用:3
  • 點閱點閱:211
  • 評分評分:
  • 下載下載:28
  • 收藏至我的研究室書目清單書目收藏:4
 運動訓練能刺激生物體提昇抗氧化活性,降低自由基的傷害。然而激烈運動後,肌肉的疼痛與自由基的產生有關聯。本研究針對二十七位受試者(13位足球選手與14位不習慣運動者),分為運動員組與非運動員組,區分為運動前組與運動後組,運動前組先採血及實施五千公尺跑步後(運動後組)採血,探討運動選手與不習慣運動者於運動前後,血液中麩胱甘、麩胱甘過氧化、超氧化岐化、丙二醛、血糖、血脂肪、NO、乳酸、肝、腎功能變化情形,目的在探討運動習慣與高度運動對人體,抗氧化能力的影響,結果顯示運動員組運動前後MDA(1.43±0.55,0.95±0.54)(P<0.05)、GSH-PX(4.10±1.40,2.60±0.80)(P<0.02)、LA (4.22±0.79,12.13±6.1)(P<0.01)、creatinine(1.67±0.1,1.47±0.2)(P<0.01)、LDH(371.1±55,430.5±74)(P<0.05)、glucose(85.5±79,109.0±22)(P<0.05)有顯著變化。
非運動員組運動前後SOD(120.95±7.96,137.38±19.19)(P<0.01)、creatinine(1.12±0.1,1.55±0.2)(P<0.01)、uric acid(6.88±1.0,7.67±1.0)(P<0.05)、glucose(78.2±9.1,125.0±2.6)(P<0.01)、Albumin(4.8±0.2,5.1±0.2)(P<0.01)有顯著變化。
運動前組運動員與非運動員比較GSH(4.16±0.60,11.70±7.50)(P<0.01)、SOD(137.74±14.59,120.95±7.96)(P<0.01)、MDA(1.43±0.55,0.26±0.02)(P<0.01)、NO(6.55±1.351,23.2±5.12)有顯著變化(P<0.01),Chol(171±20,143±28)、HDL(63.58±12,51.28±8.3)(P<0.01)、LDL(100±22,78.5±23)(P<0.02)有顯著變化。
運動後組,運動員與非運動員比較GSH(4.53±0.68,11.79±2.60)、MDA(0.95±0.54,0.25±0.23)、GSH-PX(2.60±0.80,4.00±0.90)(P<0.01)、LA(12.13±6.1,16.3±2.56)(P<0.02)、NO(6.146±0.936,16.3±2.56)(P<0.01)、HDL(66±13,53.6±8.3)(P<0.01)、LDL(100±22,79±22)(P<0.01)、Uric acid(8.7±1.7,7.67±1.0)(P<0.05)有顯著變化。
進一步實驗採用CD-1(ICR)小白鼠,依運動(游泳)不同強度分成四組(control、dose-1、dose-2、dose-3)分別實驗分析項目,在小白鼠血清脂質MDA、LA變化及小白鼠器官(肝、腎、心、肺)抗氧化如麩胱甘過氧化、超氧化化活性,結果顯示肝臟、腎臟、心臟、肺臟抗氧化如GSH-PX、SOD。結果顯示在肝臟、GSU-PX control與dose-1、control與dose-2、control與dose-3比較有顯著變化(P<0.01),在肺臟GSU-PX dose-1與dose-2、dose-2與dose-3比較有顯著變化(P<0.01),在腎臟SOD control與dose-2比較有顯著變化(P<0.01),LA方面dose-2與dose-3有顯著變化(P<0.01)。
在CD-1小白鼠肝臟結果發現訊息傳遞明顯增加,包括P38、JNK1、JNK2、Raf、PKB/AKT,我們運用西方墨點吸漬法測定,發現運動後。在Raf方面四種不同運動強度變化很大,JNK1、JNK2逐漸下降,P38亦是,PKB/AKT逐漸上升。顯示在訊息傳遞蛋白表現在運動上扮演重要角色。
Training increases the antioxidant activity, thereby protecting tissues from the damage caused by free radical. Free radicals are probably involved in the development of post-exercise muscle soreness.
The study in connection with twenty-seven person (13 person football team and 14 person non-sportsman), to differentiate between sportsman group and non-sportsman group, to differentiate between before-exercise group and to run five thousand meter group (exercise group), to different sampling blood. explore sportsman and non-sportsman at before-exercise and ofter-exercise, in blood glutathione (GSH), glutathione peroxidase (GSH-PX), superoxidedismutase (SOD), malondiadehyde (MDA), blood sugar, bloot fat, nitric acid (NO), latic acid (LA) and liver/renal function to change situtation, the purpose of this study is to compare the antioxidant enzyme activities affect in have habit of exercise and height exercise to human.
The change obvious shown in result before/after exercise of the sportsmen group on MDA (1.43±0.55, 0.95±0.54) (P<±0.05), GSH-PX (4.10±1.40, 2.60±0.80) (P<0.02), LAC(4.22±0.79, 12.13±6.1) (P<0.01, creatinine (1.67±0.1, 1.47±0.2) (P<0.01), LDH (371.1±55, 430.5±74) (P<0.05), glucose (85.5±79, 109.0±22) (P<0.05).
The change has shown obviously before/after exercise of non-sportsmen group on SOD (120.95±7.96, 137.38±19.19) (P<0.01), creatinine (1.12±0.1, 1.55±0.2) (P<0.01), uric acid (6.88±1.0, 7.67±1.0) (P<0.05), glucose (78.2±9.1, 125.0±2.6) (P<0.01), Albumin (4.8±0.2, 5.1±0.2) (P<0.01).
T make a comparison between sportsmen group and non-sportsmen group before exercise, it change obviously on GSH (4.16±0.60, 11.70±7.50) (P<0.01), SOD (137.74±14.59, 120.95±7.96) (P<0.01), MDA (1.43±0.55, 0.26±0.02) (P<0.01), NO (6.55±1.351, 23.2±5.12), and also change obvious on (P<0.01), Chol (171±20, 143±28), HDL (63.58±12, 51.28±8.3) (P<0.01), LDL (100±22, 78.5±23) (P<0.02).
And in a comparison between sportsmen group and non-sportsmen group before exercise, it change obviously on GSH (4.53±0.68, 11.79±2.60), MDA (0.95±0.54, 0.25±0.23), GSH-PX (2.60±0.80, 4.00±0.90) (P<0.01), LA (12.13±6.1, 16.3±2.56) (P<0.02), NO (6.146±0.936, 16.3±2.56) (P<0.01), HDL (66±13, 53.6±8.3) (P<0.01), LDL (100±22, 79±22) (P<0.01), Uric acid (8.7±1.7, 7.67±1.0) (P<0.05).
For further examination with CD-1 (ICR) minc, we divided into 4 teams (control, dose-1, dose-2, dose-3) according to the different strength of the sport (swimming) to examine and analyze the items of MDA of serum lipid preoccupation, LA, and organs (e.g. :liver, kidney, heart, and lung), antioxidant enzyme, e.g. :GSH-PX, SOD, and it shown that in the liver, change more obviously (P<0.01) to GSH-PX in control & dose-1, control & dose-2, control & dose-3; and in lung, change more obviously to GSH-DX in dose-1 & dose-2, dose-2 & dose-3; in kidney, change more obviously (P<0.01) to SOD in contrl & dose-2; and change more obviously (P<0.01) for LA in dose-2 & dose-3.
We found the signal transudation protein increased obviously in CD-1 mice’s liver, include P38, JNK1, JNK2, Raf, PKB/Akt, we checked with westernblot test, and we found 4 different sport strength not changed obviously in Raf after exercise, JNK1, JNK2 down gradually, P38 lowered, too, PKB/AKT up gradually. Therefore, signal transudation protein acts an important part in exercises.
縮 寫 表 1
中文摘要 2
Abstract 4
緒 論 7
一、氧化壓力(oxidative stress) 7
二、自由基的產生 8
三、細胞組織氧化傷害 8
四、脂質過氧化 9
五、生物體內的抗氧化系統 10
(一)超氧化岐化(superoxide dismutase, SOD) 11
(二)麩胱甘(glutathione, GSH) 12
(三)麩胱甘過氧化(glutathione peroxidase, GSH-Px) 13
六、一氧化氮NO(nitric oxide) 14
七、血乳酸(lactic acid) 16
八、ROS在蛋白質磷酸化中的作用 19
(一)酪氨酸-磷酸化 19
(二)絲氨酸/蘇氨酸-磷酸化 19
九、p38MAPK信號通路 20
十、Ras/Raf/MEK/Erk通路調控細胞功能的機制 21
十一、Raf與MAPKs之關係 22
十二、PKB的信息傳遞作用 22
十三、MAPK訊息傳遞蛋白與運動關係 23
研究目的及動機 25
壹、儀器設備及材料 26
一、實驗對象 26
二、實驗材料 26
(一)儀器設備 26
(二)材料 27
三、實驗處理 27
(一)運動分組及採樣 27
(二)動物來源 28
(三)測試方法及分組採樣 28
貳、實驗方法 29
A.脂質過氧化(MDA)測定 29
B.GSH含量分析 29
C.蛋白質(protein)濃度測定 31
D.超氧化歧化(SOD)分析 31
E.GPx活性分析 32
F.NO的測定方法 33
G.乳酸測定: 35
I.蛋白質樣本之轉印(protein transfer): 37
J.免疫墨點法(immunoblot): 37
參、統計分析 39
肆、結果 40
一、運動對血清SOD活性的影響 40
二、運動對血清GSH含量的影響 40
三、運動對血清與器官GPx活性的影響 40
四、運動與MDA的關係 41
五、運動與NO的關係 41
六、運動對肝、腎功能血清生化值影響 41
七、運動對血脂肪、血糖影響 42
八、運動對小白鼠組織SOD及GSH-PX之影響 42
九、運動對小白鼠乳酸及MDA之影響 42
十、運動小白鼠肝臟對Raf蛋白的影響 43
十一、運動對JNK(1/2)蛋白的影響 43
十二、運動對P38蛋白的影響 43
十三、運動對PKB/AKT蛋白的影響 44
伍、討論 45
一、運動對血漿中自由基代謝及抗氧化的影響 45
二、運動對抗氧化的影響(88) 47
三、運動對SOD活性的影響 51
四、運動對GSH含量的影響 52
五、運動對GPx活性的影響 53
六、運動後血清生化值的影響 53
參考文獻 69
附圖表
FIGURE 1 84
FIGURE 2 85
FIGURE 3 86
FIGURE 4 87
FIGURE 5 88
TABLE 1 89
FIGURE 6 90
FIGURE 7 91
FIGURE 8 92
FIGURE 9 93
FIGURE 10 94
1.Chow CK. (1991) Vitamin E and oxidative stress. Free Rad. Biol. Med., 11:215~232.
2.Cadenas. E.(1989) Biochemistry of oxygen toxicity. Annu. Rev. Biochem., 58:79-110.
3.Richards. D.M., Dean. R.T. and Jessup. W.(1988) Membrane proteins are critical targets in free radical mediated cytolysis. Biochem. Biophys. Acta., 946:281-288.
4.Schuessler. H. and Schilling. K.(1984) Oxygen effect in the radiolysis of protein. part 2. Bovine serum albumin, Intl. J.Radiat. Biol., 45:267-287.
5.Dean. R.T, Thomas. S.M. and Garner, A.(1986) Free-radical-mediated fragmentation of monoamine oxidase in the mitochondrial membrane. Roles of lipid radical. Biochem. J., 240:489-494.
6.Dean, R.T., Hunt, J.V., Grant, A.J., Yamamoto, Y. and Niki, E.(1991) Free radical damage to proteins: the influence of the relative localizatin of radical generation, antioxidants and target proteins. Free Rad. Biol. Med., 11:161-168.
7.Dean, R.T. and Cheeseman, K.H. (1987) Vitamin E protects proteins against free radical damage in lipid enviroments. Biochem. Biophys. Res. Commun., 148:1277-1282.
8.Takenaka, Y., Miki, M., Yasuda, H. and Mino, M. (1991) The effect of α-tocopherol as an antioxidant on the oxidation of membrane protein thiols induced by free radicals generated in different sites. Arch. Biochem. Biophys., 285:344-350.
9.Chance B. Sies H and Boveris A (1979) Hydroperoxide metabolism in mammalian organs. Physiol Rev.,59: 527-605.
10.Klebanoff SJ.(1980) Oxygen metabolism and the toxic properties of phagocyes..Ann. Intern. Med., 93:480-489.
11.Gilbert W.(1981) DNA Seguencing and gene structure, Science 214:1305-1312.
12.Deiss A, Lee GR and Gartwright GE (1970) Hemolytic anemia in Wilson''s disease. Ann. Intl. Med., 73:413-418.
13.Horvarth pM and Ip C. (1983) Synergistric effect of vitamin E and Selenium in the chemoprevention of mammary carcinogenesis in rats. Cancer Res., 43:5335-5341.
14.Ben-zeev A, Duerr A, Solomon F and penman S.(1979). The outer boundary of the cytoskeleton: A lamina derived from plasma membrane proteins. Cell 17:859-865.
15.Richards DM, Dean RT and Jessup W. (1988) Membrane proteins are critical targets in free radical mediated cytolysis. Biochim. Biophs. Acta., 946:281-288.
16.Cadenas S, Rojas C, Mendez J, Herrero A and B arja G. (1996) Vitamin E decreases urine lipid peroxidation produrts in young healthy human volunteers under normal conditions. Pharmacol. Toxicol., 79:247-253.
17.Schuessler H and S chilling K. (1984) Oxygen effect in the radiolysis of protein, Part 2, Bovine serum albumin. Intl J. Radiat, Biol., 45:267-287.
18.Dean RT, Thomal SM and Garner A. (1986) Free-radica lmediated fragmentation of monoamine oxidase in the mitochordrial mambrane, Role of Lipid radical. Biochem. J, 240:489-494.
19.Jacobson BS. (1983) Interaction of the plasma membrane with the cyto skeleton: an overriew. Tissue & Cell 15:852-859.
20.Schlessinger J. (1983) Mobilities of cell-membrane proteins; How are they modulated by the cytonskeleton? Trends Neurosci., 6:360-363.
21.Chow Ck. (1979) Nutritional influence on cellular antioxidant defense system. Am. J. Clin. Nutr., 32:1066-1081.
22.Halliwell B and Gutteridge JMC. (1990) Pole of free radicals and catalytz metal ions in human disease: an overview. Methods Enzymol 186:1-85.
23.Cotgreave IA, Moldeus Pand Orrenius S. (1988) Host biochemical defense. mechanisms against prooxidants. Annu. Rev. pharmacol. Toxicol., 28:189-212.
24.Fridovich I. (1995) Superoxide radical and superoxide dismutases. Annu. Rev. Biochem., 64:97-112.
25.Jacob RA. (1995) The integrated antioxidant system Nutr. Res. 15:755-766.
26.Kosower NS and Kosower EM. (1978) The glutathione status of cell. Intl. Cytol. 54:109-160.
27.Reed D.J. (1990) Glutathione: Toxicological implications, Annu. Rev. pharmacol. Toxicol., 30:603-631.
28.Neal GE, Nielschu, Judoh DJ and Hulbert PB (1987) Conjugation of model substrates or microsomally-activated aflatoxin Bl with reduced glutathione, catalysed by cytosolic glutathione-S-transferases in livers of rats, mice and guinea pigs. Biochem. Pharmacel., 36:4269-4276.
29.Skreydlewska E and Farbiszewsk iR. (1997) Glutathione consumption and in activation of glutathione-related enzymes in liver, erythrocytes and serum of rats atter metanel intoxication. Arch. Toxicol., 71:741-745.
30.Cross CE et al: oxygen radicals and human disease. Annals of International Medicine., 1987: 107: 526-545.
31.自由基醫學:陳瓊、周玫編著。
32.Watson RR et al: Selenium and Vitamins A, E and C: Nutrients with cancer prevention properties. J. Am. Diet Assoc., 1986; 86:505-510.
33.Diplock AT: Antioxidant nutrients and disease prevention and overview. Am. J. Clin. Nutr., 1991; 53:489s-93s.
34.Bunker VW et al: Selenium status in disease; the role of selenium as a therapeutic agent. BJCP., 1990; 44:401-3.
35.Burk RF: Molecular biology of selenium, with implications for its metabolism. FASEB J., 1991; 5:2274-9.
36.Cirelli A: Serum selenium concentration and disease progress in patients with HIV infection. Clin. Biochem., 1991; 24:211-214.
37.“你的生命活力-從自由基談起”,健康世界叢書100,林天送教授著,P.40∼43, 49-50。
38.Powers, S.K. & Howley, E.T. (1997). Exercise physiology: Theory and application to fitness and performance (3rd Ed.). Dubuque, IA: Brown & Benchmark Publishers.
39.王永盛(1994)。現代運動訓練。北京:北京體育大學出版社。
40.樓迎統、陳君侃、黃容棋、王錫五(民85)。實用生理學。匯華圖書出版社。
41.丁文琴(民85)。不同運動強度恢復期女性血乳酸最高值之探討。國立台灣師範大學體育研究所,碩士論文。
42.Hultman, E. and Saholm K.(1980). Acid-base balance during exercise. Exercise and Sport Science Review 8, 41-128.
43.Itoh, Hand Ohkuwa (1991). Ammonia and lactate in the blood after short-term sprint esercise. European Joumal of Applied Physiology., 62, 22-25.
44.Fleck, S.J. & Kraemer, W.J. (1997). Designing resistance training programs (2nd ed.). Champaign, IL: Human Kinetics.
45.楊錫讓等著(民86)。運動生理學原理及應用。台北:中國文化大學出版部印行。
46.Karlsson, J., Peterson, F.B., Henriksson, J., & Knuttgen, H.G. (1975). Effect of previous exercise with arms and legs on metabolism and performance in exhaustive exercise. Journal of Applied Physiology., 38, 763-767.
47.Fitts, R.H., & Holloszy, J.O. (1976). Lactate and contractile force in frog muscle during development of fatigue and recovery. American Joumal of Physiology., 231, 430-433.
48.Stamford, B.A., Weltman, A., Moffatt, R., & Sady, S.(1981). Exercise recovery above and below the anaerobic threshold following maximal work. Journal of Applied Physiology: Respiratory /environmental and /exercise Physiology. 51, 840-844.
49.Yates, J.W., Gladden, L.B., & Cresanta, M. K.(1983). Effects of prior dynamic leg exercise on static effort of the elbow flexors. Journal of Applied Physiology: Respiratory Environmental and Exercise Physiology, 55(3). 891-896.
50.浦鈞宗、高崇玄、馮煒權(1989)。優秀運動員機能評定手冊。北京:人民體育出版社。
51.黃永任(民83)。運動科學講座。台北:八熊星出版社。
52.Gollnick, P.D., & Hermansen, L.(1973). Biochemical adaptations to exercise: anaerobic metabolism. Exercie Sports Science Review., 1, 1-43.
53.Gass, G.C., Rogers, S., & Mitchell, R.(1981). Blood lactate concentration following maximum exercise in trained subjects. British Journal of Sports Medicine., 15(3), 172-176.
54.馮煒權、翁慶章等著(1990)。血乳酸與運動訓練-應用手冊。北京:人民體育出版社。
55.Trivedi, B. & Danforth, w.H. (1966). Effect of PH on the kinetics of frog muscle phosphofructokinase. Journal of Biological Chemistry., 241, 4110-12.
56.Frank J. T. Staal, Micheael T. Anderson, Gerard E. J. Stall, Leonard A. Herzenberg, Cralos Gittler, and Leonore A. Herzenberg. Redox regulation of signal transduction: Tyrosine phosphorylation and calcium influx. Proc. Natl. Acad. Sci. USA Vol 91 pp. 3619-3622, 1994.
57.Graeme R. Guy, Jennifer Cairns, Siew Bee Ng, and Y. H. Tan. Inactivation of a Redox-sensitive Protein Phosphatase during the Early Events of Tumor Necrosis Factor/Interleukin-1 Signal Transduction. J.B.C. Vol. 268, No. 3, pp. 2141-2148, 1993.
58.Mary Ann Stevenson, Stephanie Sue Pollock, C. Norman Coleman, and Stuart K. Calderwood. X-Irradiation, Phorbol Esters, and H2O2 Stimulate Mitogen-activated Protein Kinase Activity in NIH-3T3 cells through the Formation of Reactive Oxygen Intermediates. Cancer Research 54, 12-15, 1994.
59.Stephen M. Keyse & Elizabeth A. Emslie. Oxidative stress and heat shock induce a human gene encoding a protein-tyrosine phosphatase. Nature. Vol 359. 15, 1992.
60.Eva Cano and Louis C. Mahadevan. Parallel signal processing among mammalian MAPKs. TIBS 20-March, p117-112, 1995.
61.Zhengui Xia, Martin Dickens, Joel Raingeaud, Roger J. Davis, Michael E. Greenberg. Opposing Effects of ERK and JNK-p38 MAP Kinases on Apoptosis. SCIENCE. Vol. 270. p1326, 1995.
62.Jyoti Sen, Rosana Kapeller, Ruben Fragoso, Ranjan Sen, Leonard I. Zon, and Steven J. Burakoff. Intrathymic Signals in Thymocytes Are Mediated by p38 Mitogen-Activated Protein Kinase. The American Association of Immunologists, p4535, 1996.
63.Kenneth W. Wood, Haiqing Qi, Gabriella D’Arcangelo, Robert C. Armstrong, Thomas M. Roberts, and Simon Halegoua. The cytoplasic raf oncogene induces a neuronal phenotype in PC12 cells: A potential role for cellular raf kinases in neuronal growth factor signal transduction. Proc. Natl. Acad. Sci. USA. Vol. 90. pp. 5016-5020, 1993.
64.Clodagh C. O’Shea, Tessa Crompton, Ian R. Rosewell, Adrian C. Hayday and Michael J. Owen. Raf regulates positive selection. Eur. J. Immunol, 1996, 26, p2350-2355.
65.Catrin A. Pritchard, Michael L. Samuels, Elizabeth Bosch, and Martin Mcmahon. Conditionally Oncogenic Forms of the A-Raf and B-Raf Potein Kinases Display Different Biological and Biochemical Properties in NIH 3T3 Cells. Molecular and Cellular Biology, Nov. 1995, p. 6430-6442.
66.Michael L. Samuels and Martin Mcmahon. Inhibition of Platelet-Deived Growth Factor- and Epidermal Growth Factor-Mediated Mitogenesis and Signaling in 3T3 Cells Expressing △Raf-1:ER, and Estradiol-Regulated Form of Raf-1. Molecular and Cellular Biology, Dec. 1994, p. 7855-7866.
67.Tomas Jelinke, Andrew D. Catling, Christoph W. M. Reuter, Shonna A. Moodie, Alan Wolfman, and Michael J. Weber. Ras and Raf-1 Form a signalling Complex with MEK-1 but Not MEK-2. Molecular and Cellular Biology, p. 8212-8218, 1994.
68.Jones PF, Jakubowicz T, Hemmings BA. Molecular cloning of a second form of rac protein kinase Cell Regul, 1991, 88:4171∼4175.
69.Bellacosa A, Testa JR, Staal SP, et al. A retroviral oncogene akt, encoding a serine-threonine kinase containing an SH2-like region. Science, 1991, 254:274∼277.
70.Karin M. The regulation of AP-1 activity by mitogen-activated protein kinase. J Biol Chem, 1995, 270:16483-16486.
71.Kathleen B. Schwarz. Oxidative Stress During Viral Infection: A Review. Free Radical Biology & Medicine, Vol. 21, No. 5, pp. 641-649, 1996.
72.Yagi, K. Lipid peroxidation and human diseas chem phys. Lipid., 45, 337-351, 1987.
73.Bradford. M.M. Hrapid and Sensitive method for the quantification of microgramquantities of protein utilizing the principle of protein-dye binding, And Biochem., 72 248-254, 1976.
74.Feelish M and Noack EA. Correlation between hitrin oxide formation during degradation of organce nitrate and activation of quanylate cyclase, Eur. J. pharmacol, 139, 19-30, 1987.
75.Gruetter CA, Barry BK, McNamara DB, Kadowitz PJ and Ignarro LS. Coronary arterial relaxation and guanylate cyclase activation by cigarette smoke, N’-nitroso-nornicotine and nitric oxide. J. Pharmacol. Exp. Ther., 214-9-15, 1980.
76.Wang J.F, Komarov P, Sies H and de Groot H. Contribution of nitric oxide synthesis to liminal-dependent chemiluminescence generated by phorbol-ester activated Kupffer cells. Biochem. J., 279, 311-314, 1991.
77.Furchgott RF, Cherry PD, Zawadzki JV and Jothianandan D. Endothelial cells as mediators of vasodilatation of arteries. J. Cardiovasc. Pharmacol. 6, suppl 2, S336-343, 1984.
78.Leuenberger U, Gauch R, Rieder K and Baumgartner E. Determination of nitrate and bromide in foodstuffs by High-Performance Liquid Chromatography. J. Chromatography., 202, 461-468, 1980.
79.曹國華,陳吉棣,一次有氧運動或無氧運動對人體內自由基生成與消除的影響,中國運動醫學雜誌,1991:10(1):1。
80.程吟梅等,健身跑對中老年人超氧化物歧化活力和含量的影響及其相互關係初探,中國運動醫學雜誌,1994:13(1):49。
81.顧榮瑞等,動脈粥樣硬化的機制及不同運動強度對其影響的研究,中國運動醫學雜誌,1994:13(3):65。
82.倪耀華,運動強度對血漿脂質過氧化物和超氧化物歧化活性的影響,中國運動醫學雜誌,1992:11(3):118。
83.Gee D. et al. The effect of exhaustive exercise on expired pentant as measure of in vivo lipid peroxidation in the rat. Life Sci., 1981: 28: 2425.
84.Vinikka L. et al. Lipid peroxide prostacyclin and thromboxane A2 in runner during actue exercise. Med Sci sports Exero 1984: 16(3): 275.
85.馮連世等,急性運動對血清超氧化物歧化的影響及其與有氧能力的關係,中國運動醫學雜誌,1994:13(3):129。
86.馮連世等,運動與血清活性的變化,中國運動醫學雜誌,1991:10(2):88。
87.李愛華等,劇烈運動對自由基影響的實驗研究,中國運動醫學雜誌,1991:10(2):79。
88.運動與肌纖維類型對抗氧化的影響。廖威彰/國立交通大學, 謝錦城/國立新竹師院副教授。P.18-24。
89.Jenkins, R.R., Friedland, R., & Howald, H.(1984). The relationship of oxygen uptake ot superoxide dismutase and catalase activity in human skeletal muscle. International Journal of Sport medicine, 5(1):11-14.
90.Quintanilha, A.T.(1984). Effects of physical exercise and/or vitamin E on tissue oxidation metabolism. Biochemical Society Transactions, 12:403-404.
91.Ji, L.L., Fu, R.(1992). Responses of glutathione system and antioxidant enzymes to exhaustive exercise and hydroperoxide, Journal of Applied Physiology, 72(2):549-554.
92.Ji, L.L., Frederick, W., & Lardy, H.A.(1988). Enzymatic down regulation with exercise in rat skeletal musele. Archives of Biochemistry and Biophysics, 263(1):137-149.
93.Ji, L.L., Dillon, D., & Wu, E. (1990). Alteration of antioxidant enzymes with aging in rat skeletal musele and liver. American Journal of Physiology, 258:R918-923.
94.Ji, L.L., Fu, R., & Mitchell, E.W.(1992). Glutathione and antioxidant enzymes in skeletal muscle:effects of fiber type and exercise intensity. Journal of Applied, Physiology, 73(5):P1854-1859.
95.Ji, L.L., (1993). Antioxidant enzyme response to exercise and aging. Medicine and Science in Sports and Exercise, 25(2):225-231.
96.Ji. L.L., (1995). Exercise and oxidative stress:role of the cellular antioxidant systems. Sports Medicine, 23:135-166.
97.Duthie, G.G., Robertson, J.D., Maughan, R.J., & Morrice, P.C. (1990). Blood antioxidant status and erythrocyte lipid peroxidation following distance running. Archives of Biochemistry and Biophysics, 282(1):78-83.
98.Rokitzki, L., Logemann, E., Sagredos, A.N., et al.(1994). Lipid peroxidation and antioxidative vitamins under extreme endurance stress. Aeta Physiologica Scandinavica, 151:149-158.
99.Lawler, L.M.,Power, S.K., Visser, T., Dijk, H.V.,Kordus, M.J., & Ji, L.L.(1993). Acute exercise and skeletal musele antioxidant and metabolic enzymes: effects of fiber type and age. American Journal of Physiology, 265:R1344-1350.
100.Criswell, D., Powers, S., Dodd, et al. (1993). High intensity training-induced changes in skeletal muscle antioxidant enzyme activity. Medicine and Science in Sports and Exercise, 25(10):1135-1140.
101.Kanter, M.M., Hamlin, R.L. Unverferth, D.V., et al.(1985). Effect of exercise training on antioxidant enzymes and cardiotoxicity of doxorubicin, Journal of Applied Physiology, 59(4):1298-1303.
102.Mena, P., Maynar, M., Gutierrez, J.M.,et al. (1991). Erythrocyte free radical scavenger enzymes in bicycle professional racers. adaptation to training. International Journal of Sport medicine, 12(6):563-566.
103.Ohno, H., Sato, Y., & Yamashita K.(1986). The effect of brief physical exercise on free radical scavenging enzyme systems in human red blood cells. Canadian Journal of Physiology and Pharmacology, 64:1263.
104.謝錦城(民86):人體骨骼肌抗氧化系統對於耐力運動與訓練的反應。中華民國大專院校八十六年度體育學術研討會專刊(下),中華民國大專體育總會出版,382-399頁。
105.Powers, S.K., Lawler, J., Criswell, D., et al.(1992). Aging and respiratory muscle metabolic plasticity: effects of endurance training. Journal of Applied Physiology, 72(3):1068-1073.
106.Powers, S.K., Criswell, D., Lawler, J., et al.(1994). Influence of exercise and fiber type on antioxidant enzyme activity in rat skeletal muscle. American Journal of Physiology, 266:R375-380.
107.Lecuwenburgh, C., fiebig, R., chandwanney, R., & Ji, L.L.(1994).Aging and exercise training in skeletal musele:responses of glutathione and antioxidant enzyme systems. American Journal of Physiology, 267.(2pt2):R439-445.
108.Tessier, F., Margaritis, I. Richard, M. J., et al (1995). Selenium and training effects on the glutathione system and aerobic performance. Medicine and Science in Sports and Exercise, 27(3):390-396.
109.Alessio, H.M., & Goldfarb, A.H.(1988). Lipid peroxidation and scavenger enzymes during exercise: adaptive response to training. Journal of Applied Physiology, 64(4). 1333-1336.
110.Kaner, M.M., Nolte, L. A. & Holloszy, J. O. (1993). Effects of an antioxidant vitamin mixture on lipid peroxidation at rest and postexercise. Journal of Applied Physiology, 74(2), 965-969.
111.Sjodin, B., Westing, Y.H., & Apple, F.S. (1990). Biochemical mechanisms for oxygen free radical formation during exercise. Sports Medicine, 10(4), 236-254.
112.Jenkins, R.R. (1988). Free radical chemistry: relationship to exercise. Sports Medicine, 5. 156-170.
113.馮連世、楊奎生、宗丕芳、郭軍(1994):急性運動對血清超氧化物岐化的影響及其與有氧能力的關係。中國運動醫學雜誌,13(3):129-132。
114.Bedford, T.G., Tipton, C.M., Willson, N.C., Oppliger, R.A., Gisolfi, C.V.(1979).Maximum oxygen consumption of rats and its changes with various experimental procedures. Journal of Applied Physiology, 47(6), 1278-1283.
115.Quintanilha, A.T.(1984). Effects of physical exercise and/or vitamin E on tissue oxidative metabolism. Biochemical Society Transactions, 12(3), 403-404.
116.Ji, L.L. & Fu, R. (1992). Responses of glutathione system and antioxidation enzymes to exhaustive exercise and hydroperoxide. Journal of Applied Physiology, 72(2), 549-554.
117.Ji, L.L. (1993). Antioxidant enzyme response to exercise and aging. Medicine and Science in Sports and Exercise, 25(2), 225-231.
118.謝錦城、許壬榮、呂學冠、廖威彰、劉燦榮(民87):衰竭運動對老鼠紅血球脂質過氧化物、變形性與型態學的影響。中華民國大專院校八十七年度體育學術研討會專刊(上),17-27。
119.Ji, L.L., Dillon, D., & Wu, E. (1990). Alteration of antioxidant enzymes with aging in rat skeltal muscle and liver, American Journal of Physiology, 258, R918-923.
120.Duthie, G.G., Robertson, J.D., Maughan, R. J., & Morrice, P.C.(1990). Blood antioxidant status and erythrocyte lipid peroxidation following distance running. Archives of Biochemistry and biophysics, 282(1), 78-83.
121.謝錦城(民86):人體骨骼肌抗氧化系統對於耐力運動與訓練的反應。中華民國大專院校八十六年度體育學術研討會專刊(下),167-185。
122.倪耀華(1992):運動強度對血漿脂質過氧化物和超氧化物歧化活性的影響。中國運動醫學雜誌,11(2),118-119。
123.Dufaux, B., Heine, O., Kothe, A., Prinz, U., & Rost, R. (1997). Blood glutatione status following distance running. Internation Journal of Sports Medicine, 18(2). 89-93.
124.CRC Handbook of Free Radicals and Antioxidants in Biomedicine VolumeⅡ, Consequences of Impaired antioxidant protection of the retina, Martin L. Katz and W. Gerald Robison, Jr. P.292, 1989.
125.Trace Elements, Micronutrients, and Free Radicals, Chapter 2. Free Radicals as Mediators of Tissue Injury, rolando del maestro P.30, 1991, Humana Press, Tatowa, New Jersey.
126.Aquatic toxicology Molecular, Biochemical, and cellular perspectives, chapter 2, Enzymology and Molecular Biology of phase Ⅱ xenobiotic-conjugating ehzymes in fish, P.61, 1994, Lewis Publishers.
127.Introduction to Biochemical Toxicology Second edition. Chapter 12, mechanisms of chemically induced cell injury and cellular protection Mechanisms, Donald J. Reed, P.281, 1994.
128.Chow C.K.(1991) Vitamin E and oxidatine Seress Free Radical Biol. Med. 11:215-232.
129.Oxicative enzymes in Foods, 2, Superoxide Dismutase, Judith K. Donnelly & David S. Robinson, procter Department of Food Science, The University of Leeds, Leeds, UK, P.52, 1994, Elsevier applied scienc publishers LTD.
130.Meister, A. (1988) Glutathione metabolism and its selective modification J. Biol. Chem. 263:7205-17208.
131.CRC Handbook of Free Radicals and Antioxidants in Biomedicine volume Ⅱ, Serum α-Tocopherol, coenzyme Q, and TBA-reactive substance in Acute myocardial damage and stroke, Masayoshi Kibata, P.236, 1989, by CRC Press, Inc.
132.CRC Handbook of Free Radicals and Antioxidants in Biomedicine Volune Ⅱ, Effect of vitamin E on low-density lipoprotein metabolism in the arterial wall, Yasushi Saito and Nobubiro Morisaki P.221. Cell-cell interaction through modification of LDL by oxidation mechanism, 1989, by CRC Press, Inc.
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
無相關期刊