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研究生:顏峰霖
研究生(外文):Feng-Lin Yen
論文名稱:傳統方劑與生藥之抗氧化活性資源開發研究
論文名稱(外文):The developmental study of antioxidant activities on traditional Chinese prescriptions and several folk medicines in Taiwan
指導教授:林 俊 清
指導教授(外文):Chun-Ching
學位類別:碩士
校院名稱:高雄醫學大學
系所名稱:天然藥物研究所
學門:醫藥衛生學門
學類:藥學學類
論文種類:學術論文
論文出版年:2000
畢業學年度:88
語文別:英文
論文頁數:78
中文關鍵詞:中風動脈硬化細胞色素 c黃嘌呤氧化酵素超氧陰離子自由基羥基自由基電子自旋共振
外文關鍵詞:strokeathrosclerosiscytochrome cxanthine oxidasesuperoxide radcialhydroxyl radicalElectron spin resornace
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中風是國人的十大死亡原因之一,而中風主要有四種類型:腦栓塞、腦血栓、腦出血、蜘蛛膜下腔出血,其中以血塊引起的腦栓塞、腦血栓為最常見。腦栓塞通常是在已被動脈硬化症所損壞的動脈中形成,動脈硬化症的部分成因是因高血脂中的脂肪、膽固醇和形成血塊的必要物質沉積在動脈所造成。根據醫學研究顯示,當人在面臨氧化壓力時,一旦抗氧化系統失去平衡,活性氧(自由基)便會去攻擊破壞膽固醇中的低密度脂蛋白( LDL ),使得氧化的脂質沉積在血管壁上,因而造成動脈粥狀硬化,嚴重時有變成心臟病、高血壓、中風等疾病之危險,而抗氧化物的使用,能有效改善及預防動脈硬化。為了開發中風防治生藥資源,而著手對我國傳統醫學常用方劑:血腑逐瘀湯、烏藥順氣散、補陽還五湯、小續命湯及柴胡加龍骨牡蠣湯等,進行研究其抗氧化活性之研究,以期改善及預防動脈硬化,進而對中風之治療有所助益。實驗是以Cytochrome C還原比色法測定各方劑清除•O2- 自由基之效果,結果發現此五種方劑均具有清除•O2-自由基的活性,其中以小續命湯最具效果。而在抑制自由基的形成,則以柴胡加龍骨牡蠣湯最具效果。另以Hypoxanthine-xanthine oxidase和Fenton反應體系分別產生超氧陰離子自由基Superoxide radical和Hydroxyl radical,以5,5-Dimethyl-1-pyrroline-1-oxide作為spin adduct捕捉劑,並以電子自旋共振 ( Electron spin resonace¸ESR ) 技術直接觀察生藥方劑對氧自由基的清除作用,結果發現此五種方劑均具有清除•O2- 及•OH自由基的活性,其中以柴胡加龍骨牡蠣湯清除•O2-最具效果,清除•OH自由基方面則以補陽還五湯最具效果,因此更進一步證實此五種方劑具有清除自由基的能力。此外,五種方劑在氯化亞鐵-抗壞血酸-誘發脂質過氧化實驗均具有抗脂質過氧化的能力活性,其中以血腑逐瘀湯較佳。而在抗高血脂作用實驗中也確認五種方劑都具有降低 LDL 及 Cholesterol 的作用,其中以血腑逐瘀湯較佳。
綜合以上的研究結果,血腑逐瘀湯、烏藥順氣湯、補陽還五湯、小續命湯及柴胡加龍骨牡蠣湯等五方劑均具有良好之清除自由基之作用及降血脂能力。
此外還針對台灣產市售生藥細葉山葡萄、杭菊、洛神花、白鶴靈芝、苧麻、藿香、紫蘇、麥門冬、絞股藍、香蘭、仙草、白鳳豆、愛玉、魚腥草、靈芝、鳳尾草、一條根等十七種作一完整的抗氧化活性探討。以Cytochrome C還原比色法測試清除‧O2- 自由基的能力及利用xanthine oxidase inhibition試驗來測試抑制‧O2- 自由基的能力;最後再以TBA法來評估對於因自由基所導致的脂質過氧化情形是否具有抑制作用。在清除‧O2- 自由基的能力中實驗結果顯示,在原生藥抽取物,除了麥門冬、白鳳豆、一條根不到50 %的抑制作用外,其餘生藥都具有50 %的抑制作用,以杭菊、白鶴靈芝、靈芝及細葉山葡萄最具效果。另外在抑制‧O2- 自由基的能力實驗結果顯示,以洛神花、愛玉、細葉山葡萄、杭菊較具抑制效果,其餘生藥並無明顯的具有抑制自由基形成的作用。在氯化亞鐵-抗壞壞血酸誘發脂質過氧化試驗中的結果顯示,靈芝及苧麻都確實有較強的抑制脂質過氧化的作用活性,而杭菊、愛玉、絞股藍、細葉山葡萄次之,其餘生藥之白鳳豆、麥門冬及一條根都不具抑制作用。
The generation of oxygen radicals and the process of lipid peroxidation may be a factor in the cerebral damage secondary to the ischemia of the cerebrovascular disease (CVD),for example, stroke. The antioxidant activities of five traditional Chinese medicinal prescriptions: Shiee-Fuu-Jwu-Iu-Tang (SFJIT), Oh-Yaw-Shuen-Chin-Saan (OYSCS), Buu-Yang-Hwan-Wuu-Tang (BYHWT), Sheau-Shiuh-Ming-Tang (SSMT) and Chir-Hwu-Jia-Long-Guu-Muu-Lih-Tang (CHJLGMLT) were investigated in anti-lipid peroxidation, anti-superoxide formation and free radical scavenger activity, which were examined by means of FeCl2-ascorbic acid induced lipid peroxidation, xanthine oxidase inhibition, cytochrome C system and an electron spin resonance(ESR) spectrometer, respectively.
The results showed that CHJLGMLT has more anti-lipid peroxidation and anti-superoxide formation effects than other prescriptions, CHJLGMLT showed the greatest superoxide radical scavenger activity and BYHWT showed the greatest hydroxyl radical scavenger activity.It is also proved that these prescriptions decreased the level of total cholesterol and LDL-cholesterol in serum on high cholesterol-fed rats. From these results, it seems likely that these prescriptions may be effective in the prevention and therapy of stroke which formed in the ischemic patients.
Vitis thunbergii, Chrysanthemum morifloium, Hibiscus sabdariffa Rhinacanthus nasutus, Boehmeria nivea, Agastache rugosa, Peerilla frutescens Britten var, Luffa cylindrica, Gynostemma pentaphyllum, Haraella retrocalla, Mesona procumbens, Cacavalia gladiata, Ficus pumil, Houttuynia cordata, Ganoderma lucidum and Pteris ensiformis, are folk medicine in Taiwan, were investigated in anti-lipid peroxidation, anti-superoxide formation and free radical scavenger activity, which were examined by means of FeCl2-ascorbic acid induced lipid peroxidation, xanthine oxidase inhibition, cytochrome C system, respectively.
The results showed that Gynostemma pentaphyllum, Vitis thunbergi, Chrysanthemum morifloium, Boehmeria nivea, have more anti-lipid peroxidation and superoxide radical scavenger effects than other crude drugs. Hibiscus sabdariffa, Ficus pumil, Vitis thunbergi, Chrysanthemum morifloium showed the greatest anti-superoxide formation activity.
目 錄

中文摘要-------------------------------------------------Ⅰ
英文摘要-------------------------------------------------Ⅲ
第一篇 常用治療中風方劑之抗脂質過氧化、抗高血脂作用及抗氧化活性之研究
第一章 緒言---------------------------------------------1
第二章 抗脂質過氧化作用之研究
第一節 前言------------------------------------------ 2
第二節 實驗材料與方法-------------------------------- 2
第三節 實驗結果與討論-------------------------------- 9
表格及附圖--------------------------------------------11
第三章 自由基清除作用之研究
第一節 前言------------------------------------------14
第二節 實驗材料與方法--------------------------------14
第三節 實驗結果與討論--------------------------------18
表格-------------------------------------------------- 20
第四章 自由基生成抑制作用之研究
第一節 前言------------------------------------------21
第二節 實驗材料與方法--------------------------------22
第三節 實驗結果與討論--------------------------------25
表格-------------------------------------------------- 27
第五章 電子自旋共振法
第一節 前言------------------------------------------28
第二節 實驗材料與方法--------------------------------28
第三節 實驗結果與討論--------------------------------34
表格及附圖--------------------------------------------38
第六章 抗高血脂作用之研究
第一節 前言-----------------------------------------48
第二節 實驗材料與方法-------------------------------48
第三節 實驗結果與討論-------------------------------50
表格-------------------------------------------------51
第二篇 臺灣產生藥抗氧化活性之研究探討
第一章 緒言-------------------------------------------53
第二章 抗脂質過氧化作用之研究
第一節 前言-----------------------------------------54
第二節 實驗材料與方法-------------------------------54
第三節 實驗結果與討論-------------------------------56
表格------------------------------------------------- 58
第三章 自由基清除作用之研究
第一節 前言-----------------------------------------61
第二節 實驗材料與方法-------------------------------61
第三節 實驗結果與討論-------------------------------62
表格------------------------------------------------- 64
第四章 自由基生成抑制作用之研究
第一節 前言-----------------------------------------65
第二節 實驗材料與方法-------------------------------65
第三節 實驗結果與討論-------------------------------66
表格------------------------------------------------- 68
總結論--------------------------------------------------- 69
參考文獻------------------------------------------------- 72
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