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研究生:吳崇維
研究生(外文):Chung-Wei Wu
論文名稱:以聚合連鎖反應偵測口腔鱗狀上皮細胞癌組織之人類疣瘤病毒及EB病毒之研究
論文名稱(外文):Detection of Human papillomavirus and Epstein- Bar virus in oral squamous cell carcinoma by using the polymerase chain reaction method
指導教授:謝天渝謝天渝引用關係張基隆張基隆引用關係
指導教授(外文):TIEN-YU SHIEHKEE-LUNG CHANG
學位類別:碩士
校院名稱:高雄醫學大學
系所名稱:牙醫學研究所
學門:醫藥衛生學門
學類:牙醫學類
論文種類:學術論文
論文出版年:2000
畢業學年度:88
語文別:中文
論文頁數:68
中文關鍵詞:人類疣瘤病毒EB病毒口腔鱗狀上皮細胞癌聚合連鎖反應
外文關鍵詞:Human papillomavirusEpstein- Bar virusoral squamous cell carcinomapolymerase chain reaction
相關次數:
  • 被引用被引用:5
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  • 下載下載:16
  • 收藏至我的研究室書目清單書目收藏:0
本研究主要是利用聚合鏈反應(polymerase chain reaction, PCR)來探討50個口腔鱗狀細胞癌病例及20個正常人與HPV-16及EBV感染之間的關係。本研究的結果,正常人樣本呈HPV-16陽性反應為10%,而EBV也同樣為10%,HPV-16及EBV雙重感染的比率為5%。口腔鱗狀細胞癌樣本之HPV-16的感染率以有、無嚼食檳榔習慣來區分,有嚼食檳榔習慣者的HPV-16感染率為34%,無嚼食檳榔習慣者的HPV-16感染率為17% ,全部口腔鱗狀細胞癌樣本的感染率為32%。不論有無嚼食檳榔之習慣與正常者比較其結果在統計學上皆無顯著性差異。在EBV感染方面,有嚼食檳榔習慣者的EBV感染率為50%,無嚼食檳榔習慣者的EBV感染率為33%,全部口腔鱗狀細胞癌樣本的感染率為48%,有嚼食檳榔習慣者與正常者比較其結果在統計學上有明顯差異(p=0.002)。在HPV-16及EBV雙重感染方面,有嚼食檳榔習慣者的感染率為18%,無嚼食檳榔習慣者的EBV感染率為17%,全部樣本的感染率為18%,不論有無嚼食檳榔之習慣與正常者比較其結果在統計學上皆無明顯差異。
此外,發現在4個頸部淋巴結組織中都可偵測到HPV-16及EBV這兩種病毒的存在,HPV-16存在的比率為50%,而EBV存在的比率更高達100%,其中HPV-16及EBV共同存在率為50%。在無嚼食檳榔習慣之復發腫瘤的組織中EBV存在比率也高達100%。
因此EBV的感染對於嚼食檳榔者之口腔黏膜的癌化可能具有某些作用,而 HPV-16或EBV的感染對於頸部淋巴結的轉移及在無嚼食檳榔習慣腫瘤的復發,可能具有某些意義,值得我們再做更進一步的研究。
The purpose of this study was to understand the relationship between 50 specimens of oral squamous cell carcinoma or 20 specimens of gingiva and HPV-16 and EBV infection by using polymerase chain reaction method. Our results showed that HPV-16 infection rate of normal gingiva was 10%, and the EBV infection rate is also 10%. The oral cancer patients were divided into one group with betel nut chewing habit and the other group without betel nut chewing habit. HPV-16 DNA could be identified in 15 out of 44 (34%) oral cancer specimens from patients with betel nut chewing habit and in 1 out of 6 (17%) oral cancer specimens from patients without betel nut chewing habit. The overall HPV-16 infection rate in oral cancer specimens was 32% (16/50). There was no significant difference in HPV-16 infection rate between oral cancer specimens and normal gingiva specimens. EBV DNA could be detected in 22 out of 44 (50%) oral cancer specimens from patients with betel nut chewing habit and in 2 out of 6 (33%) oral cancer specimens from patients without betel nut chewing habit. The overall EBV infection rate in oral cancer specimens was 48%. There was a significant difference in EBV infection rate between oral cancer specimens either from patients with betel nut chewing habit or from patients without betel nut chewing habit and normal gingiva specimens.
We also found the presence of HPV-16 or EBV DNA in 4 cancerous tissue specimens from metastatic lymph nodes. The HPV-16 DNA positive rate in metastatic lymph nodes was 50% and the EBV DNA positive rate in mrtastatic lymph nodes was 100%. In addition, EBV DNA could be detected in all the recurrent cancers from patients without betel nut chewing habit.
Our results suggest that EBV may play a role in oral carcinogenesis in patients with betel nut chewing habit. Furthermore, HPV-16 or EBV may be associated with cervical lymph node metastases and with oral cancer recurrence in patients without betel nut chewing habit. Further studies are needed to prove these hypotheses.
目 錄
壹、 摘要---------------- 1
中文摘要---------- 2
英文摘要---------- 4
貳、 緒論---------------- 5
參、 文獻回顧----------- 12
肆、 研究方法----------- 20
伍、 結果--------------- 31
陸、 討論--------------- 41
柒、 參考文獻----------- 48
捌、 圖表--------------- 57
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