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研究生:江宏仁
研究生(外文):Chiang, Hung-Ren
論文名稱:培養中巨噬細胞對於鈷鉻鉬生醫材料表面特性發炎反應之變化
論文名稱(外文):Changes in Inflammatory Profiles of Cultured Macrophages in Response to Surface Properties of Co-Cr-Mo Implant Alloy
指導教授:連雙喜宋晏仁宋晏仁引用關係
指導教授(外文):Shuang Shii LianYen-Jen Sung
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:材料科學與工程學研究所
學門:工程學門
學類:材料工程學類
論文種類:學術論文
論文出版年:2000
畢業學年度:88
語文別:中文
論文頁數:77
中文關鍵詞:鈷鉻鉬生醫材料
外文關鍵詞:Co-Cr-Mobiomaterials
相關次數:
  • 被引用被引用:4
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  • 下載下載:76
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摘要
生醫材料在植入人體內後之生物相容性好壞,與材質之表面特性直接相關。對於一般生醫金屬材料,國外最近興起一股重新將金屬對金屬關節接面應用在人工髖關節的研究趨勢與評估,原因是過去由於金屬表面加工技術未臻非常成熟以及材料製程等問題,使得商業上所使用之生醫金屬材料會對人體產生嚴重的傷害,成為生醫金屬材料設計上之瓶頸。但隨著近年來金屬材質及加工技術顯著之進步以及表面處理的製程之快速發展,如能運用現代之合金精煉及表面處理技術,應可使金屬表面粗糙及光滑度改善,而增加其生物相容性。
職是之故,本論文嚐試利用高壓感應熔煉法來加氮及精煉人工膝關節常用之鈷鉻鉬合金,並探討高氮鈷鉻鉬合金之生物相容性。此系統包含兩個層面:(1)利用高壓感應熔煉法所熔煉不同氮含量的鈷鉻鉬合金,來討論合金氮含量對於巨噬細胞的影響;(2)採用不同研磨方法研磨鈷鉻鉬合金,用來探討合金表面對於鼠巨噬細胞株RAW264.7發炎反應及黏附的行為。實驗中將RAW264.7細胞與鈷鉻鉬金屬共同培養並加入細菌內毒素LPS刺激,然後測試RAW264.7細胞所釋出的一氧化氮(NO)、細胞激素(TNF-及IL-1)作為細胞發炎反應的指標。結果發現鈷鉻鉬合金的氮含量並不會增加RAW264.7細胞的NO及細胞激素產量。而在合金表面粗糙度的影響方面,則利用砂輪機所研磨的粗糙合金表面與鑽石粉研磨的細緻合金表面處理組相較。結果顯示,細緻表面的合金會增加RAW264.7細胞合成NO及細胞激素,表示了合金表面性質確實在巨噬細胞的發炎反應中,扮演重要的調控角色。總結這些發現,本論文主張為了要降低人工植入體對於人體所造成的發炎反應,或許可以選擇在不需要對磨的植入性材料表面上,利用機械加工或合金設計的原理設計較為粗糙的材料表面,以利細胞的黏附以及降低發炎。
ABSTRACT
The biological compatibility of implant biomaterials is directly related to their surface properties. Inadequate surface processing and alloy manufacturing procedures have been attributed to the harmful effects observed after the implantation of metallic materials and, consequently, have limited the employment of these biomaterials as joint prostheses. However, recent advancement in metal processing techniques has made it possible to generate metallic biomaterials with improved surface properties that are more biocompatible. In light of the high failure rate of prosthesis using ultra-high molecular weight polyethylene (UHMWPE) in the contact interface, application of new metallic biomaterial in joint replacement surgery has been reevaluated by myriad orthopedic research centers.
The present work utilized nitrogen supplementation to cobalt-chromium-molybdenum (Co-Cr-Mo) alloys using high pressure induction melting (HPIM) to enhance metallic strength. Alloys so generated were examined for physical properties such as nitrogen content, metallic phases, and hardness. Furthermore, biocompatibility of these alloys was investigated by measuring proinflammatory responses of cultured murine macrophages in the context of different physical features, primarily nitrogen content and surface roughness. The muring macrophage cell line RAW264.7 was cultured in various Co-Cr-Mo alloys in the presence of a bacterial endotoxin, lipopolysaccharide (LPS), as proinflammatory stimulant and production of nitric oxide (NO), interleukin-1 (IL-1) and tumor necrosis factor- (TNF) by these cells was measured as indicators of inflammatory response. Within 24 hrs of culture period, nitrogen content appeared to have no effect on proinflammatory responses in these macrophages. On the other hand, surface roughness generated by diamond powder polish (smooth surface) or wheel grinding (rough surface) had significant impact on cellular responses. The rough surface was significantly more preferable than the smooth surface for macrophages to adhere. In addition, cell viability measurement indicated that these macrophages survived successfully on the rough surface while significant cell death occurred when cultured on the smooth surface. In contrast to inferior cell viability and adherence on the smooth surface, cells cultured in such condition produced significantly more amounts NO, IL-1, and TNF- in the presence of LPS stimulation. These data indicate that surface properties of implant alloys may play important role in the modulation of proinflammatory responses of macrophages. The data also support the proposition that increased roughness on the surface of prosthetic alloys where no joint contact was necessary may be beneficial for biocompatibility by reducing proinflammatory responses of adherent macrophages.
1.摘要
2.文獻回顧
3.實驗方法
4.結果與討論
5.結論
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