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研究生:陳哲宏
研究生(外文):Che-Hung Chen
論文名稱:斜紋夜盜蟲細胞株之建立
論文名稱(外文):Establishment of cell lines from Spodoptera Litura (F.)
指導教授:石正人石正人引用關係
指導教授(外文):Cheng-Jen Shih
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:昆蟲學研究所
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2000
畢業學年度:88
語文別:中文
論文頁數:61
中文關鍵詞:斜紋夜盜蟲細胞株
外文關鍵詞:Spodoptera lituraCell line
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摘要
本論文以斜紋夜蛾(Spodoptera litura)為材料,試圖從胚胎、血球、幼蟲的脂肪體及精巢等組織建立細胞株。目前已成功建立兩株細胞株,均源自於血球細胞,分別定名為SL-H1及SL-H2。自1999年4月,初代培養建立至今,皆已經過20餘代之繼代培養。本試驗所使用的培養液為TNM-FH並添加10﹪胎牛血清。在利用血球細胞為材料,建立初代培養細胞時,經常遭遇因血淋巴液引發的黑化作用,其對細胞具有毒性。為加強細胞分化及供給足量的生長物質,初代培養時的胎牛血清含量為15﹪,並添加抗氧化劑glutathion,以減緩培養液黑化的情形發生。在初代培養建立之時,可觀察到不同形態的血球細胞,包括prohemocyte、granulocyte、plasmatocyte和spherulocyte,經連續繼代培養後prohemocyte及oenocytoid占細胞族群之多數。就培養之細胞生長速率而言,二株細胞的倍增速率分別為96及120小時。就細胞核型分析,細胞之染色體為多倍體,在培養第20代時,二株細胞之細胞核染色體數目分別為65-130及76-123之間,且有隨繼代培養次數而增加之現象。以酯作同功異構電泳分析,結果顯示三株細胞株其電泳膠片上酯之染色條紋的位置及數目皆不同,具有鑑別能力,也証明本試驗所得之二株細胞並非遭實驗室既有培養取自斜紋夜蛾蛹體卵巢之SL-7B所污染。以100個逢機引子進行新建立細胞之DNA分析,經RAPD-PCR試驗分析結果顯示,大多數的供試引子均能區別SL-7B及新建立之血球細胞,但僅有UBC-53逢機引子可成功鑑別SL-H1及SL-H2細胞株。利用interlukin-1b及prolactin receptor特異引子對,進行DAF-PCR,結果可知對於新細胞株(SL-H1和SL-H2)與SL-7B有很好的區別能力,但對於本文所建立的SL-H1與-H2之間則沒有鑑別力。
Sumarry
Establishment of cell lines from Spodoptera litura (F.)
Che-Hung Chen
The aim of this thesis is to establish cell lines from Spodoptera litura to provide as host cell for S. litura nucleopolyhedrovirus replication in vitro. Primary culture was made from many tissues of S. litura, including embryos, larval haemolymph, fat body, larval testes, etc. Two cell lines established from hemocyte can be subcultured more than 20 passages, designated as IBL-SL-H1 and IBL-SL-H2. These two cell lines were derived since April 1999. The cells were maintained at 28℃ in TNM-FH medium supplemented with 10% fetal bovine serum (FBS). Melanization in the cultivation of hemocytes was a serious problem. The hemocytes were almost completely destroyed in cultures where melanization occurred to any great extent. Primary culture cell growth was improved by supplement of 15% FBS, and by addition of glutathion to prevent melanization and to improve cell adherence to the tissue culture flask. The estimated population doubling time of these cells were 96 and 120 hr in SL-H1 and SL-H2, respectively. The cultured cells were polyploid with chromosome numbers ranging from 65 to130 in SL-H1 and 76 to 123 in SL-H2 at the 20th passage. The chromosome number was increased with passages. In the primary culture, four types of cells were present, including prohemocyte, granulocyte, plasmatocyte and spherulocyte. After subculturing, prohemocyte-like and oenocytoid-like cells were more prevalent. The isozyme patterns of esterase were analyzed in two new cell lines and SL-7B established from pupal ovaries of Spodoptera litura. The results revealed that the distance and number of bands were different from each other. It demonstrated that these new cell lines were not contaminants with the SL-7B. 100 random primers were examined for DNA analysis for characterization of the cell lines. The results showed that most primers could distinguish from new cell lines to SL-7B. Only with primer UBC-53, the amplified DNA fragments could identify different in SL-H1 and SL-H2 cells. Primer sets for amplifying prolactin receptor and interlukin-1b genes were used in an attempt to generate fingerprints from SL-H1, SL-H2, and SL-7B. The result showed the fingerprint patterns of news cell lines and SL-7B were quite different. But SL-H1 and SL-H2 gave nearly identical patterns with these primer sets.
目錄
中文摘要
英文摘要
目錄…………………………………………………………………………i
圖次…………………………………………………………………………ii
壹、緒言……………………………………………………………………1
貳、往昔研究………………………………………………………………3
參、材料與方法……………………………………………………………13
一、材料………………………………………………………………13
二、方法………………………………………………………………14
(一) 條件培養液 (conditioned medium) 之配製…………14
(二) 初代培養之建立…………………………………………15
(三) 細胞株之繼代培養………………………………………16
(四) 細胞之冰凍保存與解凍培養……………………………16
(五) 新建立細胞之特徵………………………………………17
肆、結果與討論……………………………………………………………21
一、細胞株的建立……………………………………………………21
二、其他組織的初代培養……………………………………………25
三、新建立斜紋夜蛾細胞株的特性分析……………………………26
伍、引用文獻………………………………………………………………48
陸、誌謝……………………………………………………………………58
染、附錄……………………………………………………………………59
伍、參考文獻
石正人。1994。利用基因轉殖微生物以提高殺蟲活性。生物農業研究與發展研討會專刊。李國欽、高穗生和費雯綺編。5.1-5.36頁。
石正人、林仁偉。1995。斜紋夜蛾細胞株之建立。中華昆蟲15: 333-344。
石正人、胡耀中。1996。基因重組苜蓿夜蛾核多角體病毒對秋行軍蟲細胞株及斜紋夜蛾幼蟲殺蟲效力。中華昆蟲16: 1-12。
石正人、樊修秀、王重雄。1995a。斜紋夜蛾核多角體病毒台灣分離株特性之研究。植物保護學會會刊37: 157-167。
石正人、樊修秀、王重雄。1995b。紫外線對斜紋夜蛾核多角體病毒之影響。植物保護學會會刊37: 169-177。
胡耀中、羅竹芳、石正人。1994。構築含蘇力菌內毒素基因之重組昆蟲桿狀病毒。中華昆蟲14: 445-461。
貢榖紳。1984。臺灣主要蔬菜害蟲之經濟重要性變遷與防治展望。蔬菜害蟲研討會專刊:3-30頁。臺灣省政府農林廳編印。
崛健、素木得一。1910。台灣の害蟲に關すろ調查。臺灣農事試特別報告1號:152-154。
鄭允。1984。蔬菜鱗翅目害蟲之化學防治及抗藥性。蔬菜害蟲研討會專刊 100-110頁。台灣省政府農林廳編印。
歐陽盛芝、朱耀沂。1990。斜紋夜蛾 (Spodoptera litura (F.)) 生物學:Ⅱ. 成蟲壽命及交尾能力。中華昆蟲 10: 27-36。
歐陽盛芝、朱耀沂。1991。斜紋夜蛾 (Spodoptera litura (F.)) 生物學:Ⅳ. 雌蛾連續配對三日時的交尾和產卵能力。中華昆蟲 11: 39-47。
嚴奉琰、黃汝真。1977。微生物殺蟲劑NPV田間穩定性之研究。台大農學院研究報告17: 127-132。
蘇智勇。1992。利用斜紋夜蛾核多角體病毒防治斜紋夜蛾。植保會刊34: 227-234。
Beckage, N. E., J. S. Metcalf, D. J. Nesbit, K. W. Schleifer, S. R. Zetlan, and I. deBuron. 1990. Host hemolymph monophenoloxidase activity in parasitized Manduca Sexta larvae and evidence for inhibition by wasp polydnavirus. Insect Biochem. 20: 285-295.
Carter, J. B., C. Griffiths, and C. F. Edwards. 1994. Establishment of a cell line from Tipula paludosa (Diptera, Tipulidae) hemocytes and replication of T. paludosa baculovirus in vitro. J. Invertebr. Pathol. 63: 119-122.
Chakraborty, S., and S. Reid. 1999. Serial passage of a Helicoverpa armigera nucleopolyhedrovirus in Helicoverpa zea cell cultures. J. Invertebr. Pathol. 73: 303-308.
Davis, T. R., T. J. Wickham, K. A. Mckenna, R. R. Granados, M. L. Shuler, and H. A. Wood. 1993. Comparative recombinant protein production of eight insect cell lines. In Vitro Cell Dev. Biol. 29A: 388-390.
Disney, J. E., and W. J. McCarthy. (1985). A modified technique for the improved characterization of lepidopteran chromosomes from cells in culture. In Vitro Cell. Dev. Biol. 21: 563-568.
Donaldson, M., H. A. Wood, P. C. Kulakosky, and M. L. Shuler. 1999. Glycosylation of a recombinant protein in the Tn5B1-4 insect cell line: Influence of ammonia, time of harvest, temperature, and dissolved oxygen. Biotechnol. Bioeng. 63: 255-262.
Eide, P. E., J. M. Caldwell, and E. P. Marks. 1975. Establishment of two cell lines from embryonic tissue of the tobacco hornworm, Manduca Sexta. In Vitro 11: 395-399.
Ennis, T. J., and S. S. Sohi. 1976. Chromosomal characterisation of five lepidopteran cell lines of Malacosoma disstria (Lasiocampidae) and Christoneura fumiferana (Tortricidae). Can. J. Genet. Cytol. 18: 471-477.
Gelernter, W. D., and B. A. Federici. 1986. Continuous cell line from Spodoptera exigua (Lepidoptera: Noctuidae) that supports replication of nuclear polyhedrosis viruses from Spodoptera exigua and Autographa californica. J. Invertebr. Pathol. 48: 199-207.
Glaster, R. W. 1917. The growth of insect blood cells in vitro. Psyche 24(1): 1-7.
Goldschmidt, R. 1915. Some experiments on spermatogenesis in vitro. Proc. Natl. Acad. Sci. 2: 220-222.
Goodwin, R. H. 1975. Insect cell culture: improved media and methods for initiating attached cell lines from the Lepidoptera. In Vitro 11: 369-378
Goodwin, R. H., G. J. Topkins, R. R. Gettig, and J. R. Adams. 1982. Characterization and culture of virus replicating continuous insect lines from the bollworm, Heliothis zea (Boddie). In Vitro 18: 843-850.
Grace, T. D. C. 1962. Establishment of the four strains of cell from insect tissue grown in vitro. Nature 195: 788-789.
Grace, T. D. C. 1982. Development of insect cell culture. pp. 1-8. in: K. Maramorosch, and J. Mitsuhashi, eds. Invertebrate Cell Culture, Applications. Academic Press, New York.
Granados, R. R., and M. Naughton. 1976. Replication of Amsacta moorei entomopoxvirus and Autographa californica nuclear polyhedrosis virus in hemocyte cell lines from Estigmene acrea. pp. 379-389. in: E. Kurstak and K. Maramorosch, eds. Invertebrate Tissue Culture: Applications in Medicine, Biology, and Agriculture. Academic Press, New York.
Granados, R. R., and K. A. Williams. 1986. In vivo infection and replication of baculovirus. pp. 89-108. in: R. R. Granados, and B. A. Federici, eds. The Biology of Baculovirus. Vol. Ⅰ. CRC Press, Boca Raton, Florida.
Granados, R. R., L. Guoxun, A. C. G. Derksen, and K. A. McKenna. 1994. A new insect cell line from Trichoplusia ni (BTI-Tn-5B1-4) susceptible to Trichoplusia ni single enveloped nuclear polyhedrosis virus. J. Invertebr. Pathol. 64: 260-266.
Greene, A. E., and J. Charney, W. W. Nichols, and L. L. Coriell. 1972. Species identity of insect cell lines. In Vitro 7: 313-322.
Gupta, A. P. 1979. Insect hemocytes. Cambridge Univerisity Press, London. 614 pp.
Hakim, R. S., F. T. Hakim, and M. J. Loeb. 1997. Growth Maduca Sexta midgut epithelial cells in the establishment of a primary culture. pp. 19-24. in: K. Maramorosch, and J. Mitsuhashi, eds. Invertebrate Cell Culture. Science Publisher, Inc. U.S.A.
Harvey, G. T., and S. S. Sohi. 1989. Isozyme characterization of 8 hymenopteran and 20 lepidopteran cell lines. pp. 71-89. in: J. Mitsuhashi, ed. Invertebrate Cell System Applications. Vol. 1. CRC Press, Boca Raton, FL.
Hink, W. F. 1970. Established insect cell line from the cabbage looper, Trichoplusia ni. Nature 226: 466-467.
Hink, W. F. 1972. A catalog of invertebrate cell lines. pp. 363-387. in: C. Vago, ed. Invertebrate Tissue Culture. Vol. Ⅱ. Academic Press, New York.
Hink, W. F. 1991. A serum-free medium for the culture of insect cells and production of recombinant proteins. In Vitro Cell. Dev. Biol. 27A: 397-401.
Hirumi, H. and K. Maramorosch. 1964. Insect tissue culture: use of blastokinetic stage of leafhopper embyo. Science 44: 1465-1467.
Holtke, H. J., G. Sanger, C. KesSLer, and G. Schmitz. 1992. Sensitive chemiluminescent detection of digoxigenin-laveled nuleic acid: a fast and simple protocol and it’s application. Biotechniques 12: 104-113.
Imanishi, S. and Y. Ohtsuki. 1988. Characteristics of cell lines established from embryonic tissues of several races of the silkworm, Bombyx mori, cultured in vitro. J. Seric. Sci. Jpn. 57: 184-188.
Inoue, H. and J. Mitsuhashi. 1989. A new cell line from embryos of Bombyx mori. pp. 199-206. in: J. Mitsuhashi, ed. Invertebrate Cell System Applications. Vol. Ⅱ. CRC Press, Boca Raton, Florida.
Jarvis, D. L., J. A. G. W. Fleming, G. R. Kovacs, M. D. Summers, and L. A. Guarino. 1990. Use of early baculovirus promoters for continuous expression and efficient processing of foreign gene products in stably transformed lepidopteran cells. Bio/Technology 8: 950-955.
Kawai, Y. and J. Mitsuhashi. 1997. An insect cell line descrimination method by RAPD-PCR. In Vitro Cell. Dev. Biol. 33: 512-515.
Keddie, B. A., M. A. Erlandson, and G. J. Hilchie. 1995. Establishment and characterization of three Malacosoma disstria cell lines. J. Invertebr. Pathol. 66: 136-142.
Kondo, A., and S. Maeda. 1991. Host range expansion by recombination of the baculovirus Bombyx mori nuclear polyhedrosis virus and Autographa californica nuclear polyhedrosis virus. J. Virol. 65: 3625-3632.
Kurtti, T. J., and M. A. Brooks. 1970. Growth of lepidopteran epithelial cells and hemocytes in primary cultures. J. Invertebr. Pathol. 115: 341-350.
Kurtti, T. J., and M. A. Brooks. 1977. Isolation of cell lines from embryos of the cockroach, Blattela germanica. In Vitro 13: 11-17.
Kurtti, T. J., S. P. S. Chaudhary, and M. A. Brooks. 1974. Influence of physical factors on the growth of insect cell in vitro Ⅰ. Effect of osmotic pressure on growth rate of a moth cell line. In Vitro 10: 149-156.
Kurtti, T. J., S. P. S. Chaudhary, and M. A. Brooks. 1975. Influence of physical factors on the growth of insect cell in vitro Ⅱ. Sodium and potassium as osmotic pressure regulators of moth cell growth. In Vitro 11: 274-285.
Lee, S. H., and R. F. Hou. 1992. Establishment of a cell line derived from embryos of the diamondback moth, Plutella xylostella (L.). J. Invertebr. Pathol. 59: 174-177.
Levin, D. B. and J. Huang. 1999. Spodoptera littoralis type B nucleopolyhedrovirus infection of a grasshopper cell line. J. Invertebr. Pathol. 74: 184-192.
Loeb, M. J., Woods, C. W., Brandt, E. P. and Bôrkovec, A. B. 1982. Science 218: 896-898.
Luckow, V. A. 1993. Baculovirus systems for the expression of human gene product. Curr. Opin. Biotechnol. 4: 564-572.
Lynn, D. E. 1989. Methods for the development of cell lines from insects. J. Tissue Culture Methods. 12: 23-29.
Lynn, D. E. 1999. Development of insect cell lines: Virus susceptibility and applicability to prawn cell culture. Methods in Cell Science 21: 173-181.
Lynn, D. E., and H. Oberlander. 1983. The establishment of cell lines from imaginal wing discs of Spodoptera frugiperda and Plodia interpunctella. J. Insect Physiol. 29: 591-596.
Lynn, D. E., and M. Shapiro. 1999. New cell lines from Heliothis virescens: characterization and susceptibility to baculovirus. J. Invertebr. Pathol. 72: 276-280.
Lynn, D. E., E. M. Dougherty, J. T. McClintock, and M. Loeb. 1988. Development of cell lines from various tissues of Lepidoptera. pp. 239-242. in: Y. Kurroda, E. Kurstak, and K. Maramorosch, eds. Invertebrate and Fish Tissue Culture. Springer-Verlag, New York.
Market, C. L., and F. Moller. 1959. Multiple forms of enzymes: tissue, ontogenic and species specific patterns. Proc. Natl. Acad. Sci, USA 45: 753-763.
Martignoni, M. E., and Scallion, R. J. 1961. Preparation and uses of insect hemocyte monolayers in vitro. Biol. Bull. 121: 507-520.
McIntosh, A. H., J. J. Grasela, and R. L. Matteri. 1996. Identification of insect cell lines by DNA amplification fingerprinting (DAF). Insect Mol. Biol. 5(3): 187-195.
McIntosh, A. H., P. A. Andrews, and C. M. Ignoffo. 1981. Establishment of two continuous cell lines of Heliothis virescens (F.) (Lepidoptera). In Vitro 17: 649-654.
McIntosh, A. H., P. D. Christian, and J. J. Grasela. 1999. The establishment of Heliothine cell lines and their susceptibility to two baculovirus. In Vitro Cell. Dev. Biol. 35: 94-97.
McKenna, K. A., H. Hong, E. vanNunen, and R. R. Granados. 1998. Establishment of new Trichoplusia ni cell lines in serum-free medium for baculovirus and recombinat protein production. J. Invertebr. Pathol. 71: 82-90.
Mitsuhashi, J. 1966. Tissue culture of the rice stem borer, Chilo suppressalis Walker (Lepidoptera: Pyralidae). Ⅱ. Morphology and in vitro cultivation of hemocytes. Appl. Entomol. Zool. 1(1): 5-20.
Mitsuhashi, J. 1981. Establishment and some characteristics of a continous cell line derived from fat body of the cabbage armyworm (Lepidoptera, Noctuidae). Develop. Growth Differ. 23: 63-72.
Mitsuhashi, J. 1990. Comparison of zymograms of some insect cell lines by means of APIZYM system. Appl. Entomol. Zool. 25: 535.
Mitsuhashi, J., and H. Inoue. 1988. Obtainment of a continuous cell line from the larval fat bodies of the Mulberry Tiger Moth, Spilosoma imparalis (Lepidoptera: Arctiidae). Appl. Entomol. Zool. 23: 488-490.
Mochida, O., and T. Okada. 1974. A bibliography of Spodoptera spp. (Lepidoptera: Noctuidae). Misc. Bull. Kyushu Nat. Agr. Expt. Sta. No. 49 110 pp.
Nichols, W. W., C. Bradt, and W. Bowne. 1971. Cytogenetic studies on cells in culture from the class insecta. Curr. Top. Microbiol. Immunol. 55: 61-69.
Okada, M. 1977. Studies on the utilization and mass production of Spodoptera litura nuclear polyhedrosis virus for control of the tobacco cutworm, Spodoptera litura Fabricius. Bull. Chugoku Natl. Agric. Exp. Stn. Ser.-E, No. 12: 66 pp.
Olsen, G. J. and C. R. Woese. 1993. Ribosomal RNA: A key to phylogeny. FASEB J. 7: 113-123.
Pant, U., A. B. Sudeep, S. S. Athawale, and K. Banerjee. 1997. A new cell line from larval ovaries of Spodoptera litura (F.) (Lepidoptera: Noctuidae). In Vitro Cell. Dev. Biol. 33: 161-163.
Robb, D. A. 1984. Tyrosinase. pp. 207-240 in: R. Lontie, ed. Copper Proteins and Copper Enzymes. Vol. 2. CRC Press, Boca Raton, FL.
Rochford, R. 1984. Establishmnet of a cell line from embryos of the cabbage looper Trichoplusia ni (Hübner). In Vitro 20: 823-825.
Schmit, A. R., A. F. Rowley, and N. A. Ratcliffe. 1977. The role of Galleria mellonella hemocytes in melanin formation. J. Invertebr. Pathol. 29: 232-234.
Shih, C. J., and J. C. Chang. 1997a. High level expression of recombinant protein in a cell line derived from Spodoptera litura (Lepidoptera: Noctuidae). Appl. Entomol. Zool. 32: 179-188.
Shih, C. J., and J. C. Chang. 1997b. A simple media for growth Spodoptera litura (Lepidoptera: Noctuidae) cells and propagation of Autographa californica nuclear polyhedrosis virus. Appl. Entomol. Zool. 32: 589-594.
Shih, C. J., R. W. Lin, and C. H. Wang. 1997. Establishment of a cell line from Spodoptera litura (Lepidoptera: Noctuidae) and replication of S. litura nuclear polyhedrosis virus. J. Invertebr. Pathol. 69: 1-6.
Shouche, Y. S., M. S. Patole, U. Pant, S. Paranjape, and K. Banerjee. 1999. Authentication of two cell lines developed from the larval and pupal ovaries of Spodoptera litura by rRNA based methods. In Vitro Cell. Dev. Biol. 35: 244-245.
Sieburth, P. J., and J. E. Maruniak. 1988. Growth characterization of a continuous cell line from the velvetbean caterpillar, Anticarsia gemmatalis Hubner (Lepidoptera: Noctuidae). In Vitro Cell. Dev. Biol. 24: 195-198.
Smith, G. E., M. D. Summers, and M. J. Fraser. 1983. Production of human β-interferon in insect cells infect with a baculovirus expression vector. Mol. Cell Biol. 3: 2156-2165.
Sohi, S. S. 1971. In vitro cultivation of hemocytes of Malacosoma disstria Hubner (Lepidoptera: Lasiocampidae). Can. J. Zool. 49: 1355-1358.
Suryakala. 1998. Protein metabolism in the haemolymph and fat body of Spodoptera litura. Indian J. Ent. 60: 95-99.
Tabachnick, W. J., and D. L. Knudson. 1980. Characterization of invertebrate cell lines Ⅱ. Isozyme analysis employing starch gel electrophoresis. In Vitro 16: 392-398.
Takahashi, M., J. Mitsuhashi, and T. Ohtaki. 1980. Establishment of a cell line from embryonic tissues of fleshfly, Sarcophaga peregrina (Insecta: Diptera). Develop. Growth Differ. 22: 11-19.
Trager, W. 1935. Cultivation of the virus of grasserie in silkworm tissue cultures. J. Exp. Med. 61: 501-513.
Trumble, J. T., and Baker, T. C. 1984. Flight phenology and pheromone trapping of Spodoptera exigua (Hüber) (Lepidoptera: Noctuidae) in southern and coastal California. Entviron. Entomol. 13: 1278-1282.
Tsang, K. R., G. B. Ward, A. H. Hardan, P. K. Harein, M. A. Brooks, and L. Jacobson. 1985. Establishment and characterization of a cell line from embryos of the indian meal moth, Plodia interpunctella. J. Invertebr. Pahtol. 46: 180-188.
Vail, P. V. and D. L. Jay. 1973. Pathology of a nuclear polyhedrosis virus of the alfalfa looper in alternate hosts. J. Invertebr. Pathol. 21: 198-204.
Vaughn, J. L. 1994. Lepidopteran celll culture. pp. 37-50. in: K. Maramorosch and A. H. McIntosh, eds. Arthopod Cell Culture System. CRC Press, Boca Raton.
Vaughn, J. L., R. H. Goodwin, G. J. Tompkins, and P. McCawley. 1977. The establishment of two insect cell lines from the insect Spodoptera frugiperda (Lepidoptera: Noctuidae). In Vitro 13: 213-217.
Wayadande, A. C., and J. Fletcher. 1998. Development and use of an established cell line of the leafhopper Circulifer tenellus to characterize Spiroplasma citri-vector interaction. J. Invertebr. Pathol. 72: 126-131.
Williams, J. G. K., A. R. Kubelik, K. J. Livak, J. A. Rafalski, and S. V. Tingey. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18: 6531-6535.
Wu, J., G. King, A. J. Daugulis, P. Faulkner, D. H. Bone, and M. F. A. Goosen. 1989. Engineering aspects of insect cell suspension culture: a review. Appl. Microbiol. Biotechnol. 32: 249-255.
Wyatt, S. S. 1956. Culture in vitro of tissue from the silkwrom Bombyx mori L. J. Gen. Physiol. 39: 841-852.
Yang, J. D., P. Gecik, A. Collins, S. Czarnecki, H. H. Hsu, A. Lasdun, R. Sundaram, G. Muthukumar, and M. Silberklang. 1996. Rational scale-up of a baculovirus-insect cell batch process based on medium nutritional depth. Biotechnol. Bioeng. 52: 696-706.
Yunger, C. E., J. L. Vaughn, and J. Cory. 1967. Adaption of and insect cell line (Grace’s Antheraea cells) to medium free of insect haemolymph. Science 155: 1565-1566.
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