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研究生:楊正彬
研究生(外文):Cheng-Bin Yang
論文名稱:乳山羊乳房炎檢測方法之研究
論文名稱(外文):Study of mastitis diagnostic methods for dairy goats
指導教授:徐濟泰
指導教授(外文):Jih-Tay Hsu
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:畜產學研究所
學門:農業科學學門
學類:畜牧學類
論文種類:學術論文
論文出版年:2000
畢業學年度:88
語文別:中文
論文頁數:79
中文關鍵詞:乳山羊乳房炎
外文關鍵詞:Dairy goatMastitis
相關次數:
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本試驗目的在探討各種乳山羊乳房炎檢測指標之適用性。試驗動物包括撒能及阿爾拜因乳山羊。測定項目為乳量、乳脂、乳糖、乳蛋白質、體細胞數、生菌數、鹼性磷酸、乳酸去氫、鈉離子、鉀離子、電導度及鹽度。試驗一進行前述各測定項目間相關性比較,亦探討不同乳山羊品種對各測定項目相關性之影響。試驗二則是以金黃色葡萄球菌人為感染的模式,進行各種乳房炎檢測項目適用性之探討。試驗結果顯示,生菌數對數值閾值為2.8之條件下於乳房炎的判斷上有良好之敏感性(0.94)及專一性(0.89),可作為其它檢測指標可信度之依據。在撒能乳山羊中,乳量、體細胞數、體細胞數對數值、鹼性磷酸、乳酸去氫、鈉離子、鉀離子、電導度與生菌數對數值之間具有顯著相關(P<0.05),但其相關係數均不超過0.7。在阿爾拜因乳山羊中,乳量、乳脂、乳蛋白質、體細胞數、體細胞數對數值、鈉離子、鉀離子、電導度與生菌數對數值之間具有顯著相關(P<0.05),但其相關係數則不超過0.5。因此,以生菌數對數值之外的任何單一檢測項目進行乳山羊乳房炎之判斷,其準確性並不理想。試驗二之結果顯示,於金黃色葡萄球菌感染之情況下,撒能乳山羊之乳量、乳脂、乳糖、乳蛋白質、體細胞數對數值、鈉離子與生菌數對數值之間具有顯著相關(P<0.05);在阿爾拜因乳山羊中,則只有乳糖、體細胞數對數值、鈉離子與生菌數對數值之間具有顯著相關(P<0.05)。而以上之檢測項目與生菌數對數值間之相關係數亦不超過0.5。以上兩試驗結果均顯示,以生菌數對數值之外的任何單一檢測項目進行乳山羊乳房炎判斷之準確性均不理想,而且乳山羊品種及感染病原菌的不同皆會影響乳房炎檢測指標之選擇。若依乳山羊品種進行複迴歸式之計算,以納入多種檢測指標,則可提高乳山羊乳房炎判斷之準確性。
The objective of the experiments was to investigate the accuracy of mastitis diagnostic indicators for dairy goats. Saanen and Alpine dairy goats were used. Measurements in the experiments included milk yield, milk fat, lactose, milk protein, somatic cell counts (SCC), standard plate counts (SPC), alkaline phosphatase (ALP), lactic dehydrogenase (LDH), Na+, K+, electrical conductivity (EC) and salt content. In experiment 1, the correlation of various measurements was examined, and the breed effect on the correlation of measurements was investigated. In experiment 2, the accuracy of mastitis diagnostic indicators for artificial Staphylococcus aureus infected mammary gland halves were investigated. The results of experiment 1 showed that there were good sensitivity (0.94) and specificity (0.89) for Log SPC with threshold value 2.8 in mastitis diagnosis which can be a sound basis for evaluating other diagnostic indicators. The correlations of Log SPC with milk yield, SCC, Log SCC, ALP, LDH, Na+, K+ and EC were significant in Saanen goats (P<0.05), with no correlation coefficient higher than 0.7. The correlations of Log SPC with milk yield, milk fat, milk protein, SCC, Log SCC, Na+, K+, EC were significant in Alpine goats (P<0.05), with no correlation coefficient higher than 0.5. Except of Log SPC, any other single measurement for goat mastitis diagnosis had a ideal accuracy. The results of experiment 2 showed that the correlations of Log SPC with milk yield, milk fat, lactose, milk protein, Log SCC and Na+were significant in Saanen goats (P<0.05), with no correlation coefficient higher than 0.5. The correlations of Log SPC with lactose, Log SCC and Na+ were significant in Alpine goats (P<0.05), with no correlation coefficient higher than 0.5. In conclusion, it was not suitable to use any single measurement for the goat mastitis diagnosis except of Log SPC. According to the breeds of dairy goats, different multiple regression equations including multiple diagnostic indicators can be adopted for the diagnosis of dairy goat mastitis, which can be more accurate than any single measurement except of Log SPC.
目錄 ------------------------------------------------ i
表次 ------------------------------------------------ ii
圖次 ------------------------------------------------ iii
Ⅰ、摘要 -------------------------------------------- 1
Ⅱ、前言 -------------------------------------------- 2
Ⅲ、文獻檢討 ---------------------------------------- 3
一、乳房炎之發生 ---------------------------------- 3
二、乳房炎檢測方法 -------------------------------- 6
三、檢測方法之評估 -------------------------------- 17
Ⅳ、材料與方法 -------------------------------------- 20
一、試驗動物 -------------------------------------- 20
二、試驗處理 -------------------------------------- 20
三、樣品採集 -------------------------------------- 22
四、分析方法 -------------------------------------- 22
五、統計分析 -------------------------------------- 31
Ⅴ、結果與討論 -------------------------------------- 32
Ⅵ、結論 -------------------------------------------- 69
Ⅶ、附表 -------------------------------------------- 70
Ⅷ、參考文獻 ---------------------------------------- 71
IX、英文摘要 ---------------------------------------- 77
X 、作者小傳 ---------------------------------------- 79
白火城、黃森源、林仁壽編譯。1996。家畜臨床血液生化學。pp. 10, 11, 47。立宇出版社。台南市。
李素珍。1988。生乳中體細胞數與乳品質的關係。科學農業36(3 - 4):88 - 92。
李素珍、林慶文、陳茂。1994。生羊乳中體細胞數與乳汁品質之關係。中畜會誌23(3):287-294。
林春基、張勝善。1994。羊乳中體細胞數與乳質關係之研究。中畜會誌23(4):407- 418。
吳永惠。1994。第59章、乳房炎。牛病學。pp. 225-240。藝軒出版社。台北市。
吳東益。1996。山羊乳中體細胞數與乳房炎間相關性之研究。國立台灣大學畜產學研究所碩士論文。
陳建仁。1983。流行病學。pp.432 - 433。伙伴出版社。台北市。
莫展榕。1997。荷蘭牛牛乳之導電度、體細胞數、乳量與乳房健康之關係。國立台灣大學畜產學研究所碩士論文。
蔡淑貞、張睿昇、施養志、陳陸宏。1992。真空包裝食品中長桿菌屬細菌之污染。食品科學19(1):68 -74。
Anderson, M. and A. T. Andrews. 1977. Progressive changes in individual milk protein concentrations associated with high somatic cell counts. J. Dairy Res. 44:223-235.
Bogin, E. and G. Ziv. 1973. Enzymes and minerals in normal and mastitic milk. Cornell Vet. 63:666-676.
Bogin, E., G. Ziv, J. Avidar, B. Rivetz, S. Gordin and A. Saran. 1977. Distribution of lactate dehydrogenase isoenzymes in normal and inflamed bovine udders and milk. Res. Vet. Sci. 22:198-200.
Boscos, C., A. Stefanakis, C. Alexopoulos, F. Samartzi. 1996. Prevalence of subclinical mastitis and influence of breed, parity, stage of lactation and mammary bacteriological status on Coulter Counter Counts and California Mastitis Test in the milk of Saanen and autochthonous Greek goats. Small Rumin. Res. 21:139-147.
Contreras, A., D. Sierra, J. C. Corrales, A. Sanchez and J. Macro. 1996. Physiological threshold of somatic cell count and California Mastitis Test for diagnosis of caprine subclinical mastitis. Small Rumin. Res. 21:259-264.
Dulin, A. M., M. J. Paape and B. T. Weinland. 1982. Cytospin cetrifuge in differential counts of milk somatic cells. J. Dairy Sci. 65:1247-1251.
Dulin, A. M., M. J. Pappe, W. D. Schultze and B. T. Weinland. 1983. Effect of parity, stage, of lactation, and intramammary infection on concentration of somatic cells and cytoplasmic particles in goat milk. J. Dairy Sci. 66:2426-2433.
East, N. E., E. F. Birnie and T. B. Farver. 1987. Risk factors associated with mastitis in dairy goats. Am. J. Vet. Res. 48(5):776-779.
Egwu, G. O., L. T. Zaria, P. A. Onyeyili, A. G. Ambali, S. S. Adamu and M. Birdling. 1994. Studies on the microbiological flora of caprine mastitis and antibiotic inhibitory in Nigeria. Small Rumin. Res. 14:233-239.
Fernando, R. S., S. L. Spahr and E. H. Jaster. 1985. Comparison of electrical conductivity of milk with other indirect methods for detection of subclinical mastitis. J. Dairy Sci. 68 : 449-456.
Harmon, R. 1995. Mastitis and milk quality. In “Milk Quality,” F. Harding. Ed., pp.25-33. Blackie Academic and Professional. Glasgow, UK.
Hicks, C. R., R. J. Eberhart and W. M. Sischo. 1994. Comparison of microbiologic culture, an enzyme-linked immunosorbent assay, and determination of somatic cell count for diagnosing Staphylococcus aureus mastitis in dairy cows. J. Am. Vet. Med. Assoc. 204 : 255-260.
Kalogridou-Vassiliadou, D. 1991. Mastitis-related pathogens in goat milk. Small Rumin. Res. 4:203-212.
Kalogridou-Vassiliadou, D., K. Manolkidis and A. Tsigoida. 1990. Somatic cell counts in relation to infection status of goat udder. J. Dairy Res. 59:21-28.
Kitchen, B. J. 1981. Review of the progress of dairy science: bovine mastitis: milk compositional changes and related diagnostic tests. J. Dairy Sci. 48:167-188.
Kitchen, .B. J., G. Middleton, M. Salmon. 1978. Bovine milk N-acetyl-beta-D-glucosaminidase and its significance in the detection of abnormal udder secretions. J. Dairy Res. 45: 15-20. .
Lee, C. S. and P. M. Outteridge. 1981. Leucocyte of sheep colostrum, milk and involution secretion, with particular reference to ultrastucture and lymphocyte sub-populations. J. Dairy Sci. 48:225-237.
Maisi, P. 1990. Milk NAGase, CMT and antitrypsin as indicators of caprine subclinical mastitis infections. Small Rumin. Res. 3:493-501.
Maisi, P. and I. Riipinen.1988. Use of California Mastitis Test, N-acetyl-beta-glucosaminidase, and antitrypsin to diagnose caprine subclinical mastitis. J. Dairy Res. 55 : 309-314.
McDermott, M. P., H. N. Erb, and R. P. Natzke. 1982. Predictability by somatic cell counts related to prevalence of intrammary infection within herds mastitis. J. Dairy Sci. 65: 1535-1539.
Messom, G. V.-V., C. B. E. Roets, A.-M. Massart-Leën, R. Heyneman, W.D.J. Kremer and A. Brand. 1993. Classfication of newly calved cows into moderate and severe responder to experimentally induced Escherichia coli mastitis. J. Dairy Res. 60:19-29.
Needs, E. C. and M. Anderson. 1984. Lipid composition of milk from cows with experimentally induced mastitis. J. Dairy Res. 51:239-249.
Neville, M. C. 1995. Sampling and storage of human milk. In ”Handbook of Milk Composition,” R. G. Jensen. Ed., pp.63-79. Academic Press Limited, UK.
Obara, Y. 1985. Diagnosis of bovine mastitis by determination of lysosomal enzyme activity in milk. J-A-R-Q-Jap-Agric-Res-Q. Ibaraki, Japan : Tropical Agriculture Research Center, Ministry of Agric., Forestry and Fisheries. Mar 1985. v. 18 (4) p. 298-304.
Obara, Y. and M. Komatsu. 1984. Relationship between N-acetyl-beta-D-glucosaminidase activity and cell count, lactose, chloride, or lactoferrin in cow milk. J. Dairy Sci. 67: 1043-1046.
Park, Y. W. 1991. Interrelationships between somatic cell counts, electrical conductivity, bacteria counts, percent fat and protein in goat milk. Small Rumin. Res. 5:367-375.
Park, Y. W. and R. D. Humphrey. 1986. Bacterial cell counts in goat milk and their correlations with somatic cell counts, percent fat, and protein. J. Dairy Sci. 69:32-37.
Peaker, M. 1975. Recent advances in the study of monovalent ion movements across the mammary epithelium: relation to onset of lactation. J. Dairy Sci. 58 : 1042-1047.
Peaker, M. 1977. Mechanism of milk secretion: milk composition in relation to potential difference across the mammary epithelium. J. Physiol. 270 : 489-505.
Poutrel, B. and C. Lerondelle. 1983. Cell content of goat milk: California Mastitis Test, Coulter Counter, and Fossomatic for predicting half infection. 1983. J. Dairy Sci. 66:2575-2579.
Rota, A. M., C. Gonzalo, P. L. Rodriguez, A. I. Rojas, L. Martin and J. J. Tovar. 1993. Somatic cell types in goats milk in relation to total cell counts, stage and number of lactation. Small Rumin. Res. 12:89-98.
Sheldrake, R. F., R. J. T. Hoare and V. E. Woodhouse. 1981. Relationship of somatic cell count and cell volume analysis of goat’s milk to intramammary infection with coagulase-negative staphylococci. J. Dairy Res. 48:393-403.
Smith, M. C. and D. M. Sherman. 1994. Goat Medicine. Lea and Febiger, Malvern, Pennsylvania.
Sung, Y. Y., T. I. Wu and P. H. Wang. 1999. Evaluation of milk quality of Alpine, Nubian, Saanen and Toggenburg breeds in Taiwan. Small Rumin. Res. 33:17-23.
Tallamy, P. T. and H. E. Randolph. 1970. Influence of mastitis on properties of milk. V. total and free concentrations of major minerals in skimmilk. J. Dairy Sci. 43: 1386-1388.
Timms, L. L. and L. H. Schultz. 1985a. N-Acetyl-B-D-Glucosaminidase and somatic cells in goat milk. J. Dairy Sci. 68:3363-3366.
Timms, L. L. and L. H. Schultz. 1985b. N-Acetyl-B-D-Glucosaminidase in milk and blood plasma during dry and early postpartum periods. J. Dairy Sci. 68:3367-3370.
Tirard-Collet, P., J. A. Zee, L. Carmichael and R. E. Simard. 1991. A study of the microbiological quality of goat milk in Quebec. J. Food Protec. 54(4):263-266.
Upadhyaya, T. N. and A. T. Rao. 1993. Diagnosis and threshold values of subclinical mastitis in goats. Small Rumin. Res. 12:201-210.
Vihan, V. S. 1989. Determination of NAGase activity in milk for diagnosis of subclinical carprine mastitis. Small Rumin. Res. 2:359-366.
Wheelock, J. V., J. A. F. Rook, F. K. Neave and F. H. Dodd. 1966. The effect of bacterial infections of the udder on the yield and composition of cow’s milk. J. Dairy Res. 33:199-215.
Wilson, D. J., K. N. Stewart and P. M. Sears. 1995. Effects of stage of lactation, production, parity and on somatic cell counts in infected anf uninfected dairy goats. Small Rumin. Res. 16:165-169.
Zeng, S. S. 1996. Composition of goat milk standards with cow milk standards for analyses of somatic cell counts, fat and protein in goat milk. Small Rumin. Res. 21:221-225.
Zeng, S. S. and E. N. Escobar. 1995. Effect of parity and milk production on somatic cell count, standard plate count and composition of goat milk. Small Rumin. Res., 17:269-274.
Zeng, S. S. and E. N. Escobar. 1996. Effect of breed and milk production on somatic cell count, standard plate count and composition of goat milk. Small Rumin. Res., 19:169-175.
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