跳到主要內容

臺灣博碩士論文加值系統

(3.231.230.177) 您好!臺灣時間:2021/07/28 18:45
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

: 
twitterline
研究生:余俊億
研究生(外文):Chun-I Yu
論文名稱:太平洋白蝦感染桃拉症病毒後血漿之分析
論文名稱(外文):Hemoplasma Analysis of Pacific White Shrimp (Litopenaeus vannamei) Infected with Taura Syndrome Virus
指導教授:宋延齡宋延齡引用關係
指導教授(外文):Yen-Ling Song
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:動物學研究所
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2000
畢業學年度:88
語文別:中文
論文頁數:69
中文關鍵詞:太平洋白蝦桃拉症血漿
外文關鍵詞:Litopenaeus vannameiTaura syndormehemoplasma
相關次數:
  • 被引用被引用:7
  • 點閱點閱:270
  • 評分評分:
  • 下載下載:35
  • 收藏至我的研究室書目清單書目收藏:0
臺灣地區養殖太平洋白蝦(Litopenaeus vannamei)於1999年3月發生大量死亡現象,病蝦具有軟殼以及紅色色素擴散的情形,倖存的蝦子其殼上出現許多任意分布、不規則形狀、凹陷的黑化潰爛斑點。殼下的表皮組織以及結締組織發現有細胞核濃縮與破裂的情形,細胞質中有明顯的圓形包涵體(inclusion),呈現”胡椒粉”的樣子。針對病蝦採用桃拉症病毒專一性核酸探針作原位雜交診斷顯示87%的檢驗病蝦呈現桃拉症病毒陽性反應,經純化的病毒顆粒負染再於穿透式電子顯微鏡下觀察為正二十面體(icosaherdal),直徑為28-31nm。這些結果指出這一次臺灣地區白蝦大量死亡的主因為桃拉症病毒,桃拉症病毒可能經由慢性感染或帶原的蝦母由西半球傳播到東南亞的臺灣。
其次探討桃拉症病毒感染太平洋白蝦對血漿(hemoplasma)的影響,並對感染組與對照組作以下的血淋巴液分析,包括細菌凝集素效價(bacterial agglutinating titer)、抗菌活性分析(antibacterial activity)、血漿凝血蛋白含量(plasma clottable protein concentration)、血漿中血青素含量(plasma hemocyanin content)以及血漿中鎂鈣離子濃度(plasma Mg++/Ca++ level)。急性桃拉症造成太平洋白蝦死亡,人工感染後到了第六天存活率為0 %。血淋巴分析結果顯示隨著桃拉症病毒的感染,血漿中血青素和銅含量、血漿中鈣離子濃度與凝血蛋白量都下降,但血漿中鎂離子濃度於感染第二天顯著上升,至於抗菌活性分析,對照組與感染第0天均無法在血漿中測得活性,然而於感染第2和4天卻可於血漿中測得對抗哈維氏弧菌Vibrio harveyi的活性。本研究結果顯示急性桃拉症感染會改變蝦血漿中成份及抗菌活性,推測或許血淋巴的變化可以反映出蝦子健康的狀況,並提供作為疾病的診斷的依據。
In Taiwan, high mortality of cultured Pacific white shrimp, Litopenaeus vannamei, juveniles occurred within 3 days of disease onset in March, 1999. Moribund juveniles had a soft shell and a pale reddish coloration and survivors were found with multifocal, melanized, cuticle lesions on the cephalothorax. Prominent necrosis in the cuticular epithelium and subcutis had pyknotic nuclei and cytoplasmic spherical inclusion that gave lesions a peppered or buckshot-riddled appearance. In situ hybridization analyses using Taura syndrome virus (TSV)-specific cDNA probes showed that 87% of the juveniles examined were TSV positive. Negative staining of the purified viral preparations revealed the presence of icosahedral particles, 28-31nm in diameter. These results indicate that TSV is responsible for the acute mortality of white shrimp in Taiwan. TSV may have been introduced to Southeast Asia with chronically infected brooders or asymptomatic carriers imported from the Western Hemisphere.
Study was further conducted to examine the changes in hemoplasma of Pacific white shrimp L. vannamei artificially infected with TSV. Hemoplasma obtained from infected and control shrimps were analyzed for bacterial agglutinating titer, antibacterial activity, plasma clottable protein concentration, plasma hemocyanin content, plasma Mg++/Ca++ level. Acute TSV infection caused high mortality in Pacific white shrimp and the survival rate was 0 % on the day sixth post infection. Hematological results showed plasma hemocyanin content, plasma Ca++ level and clottable protein content significantly decreased as disease progressed by TSV infection. A significant increase in plasma Mg++ level was found toward second day of TSV group. There is no antibacterial activity in the plasma of both control group and the day 0 of TSV group. However, on the day second and fourth after TSV-infection, we can detect the antibacterial activity against V. harveyi in the plasma. These results indicate that some hemoplasma parameters changed after TSV infection and they probably could be useful as the indicators of healthy status.
目錄
中文摘要 ----------------------------------------------------------------- iv
英文摘要 ----------------------------------------------------------------- vi
前言 ----------------------------------------------------------------- 1
第一章 桃拉症診斷
文獻回顧 -------------------------------------------------------- 3
材料與方法 ------------------------------------------------------- 11
結果 ------------------------------------------------------- 15
討論 ------------------------------------------------------- 17
第二章 人工感染與血漿的分析
文獻回顧 ------------------------------------------------------- 19
材料與方法 ------------------------------------------------------- 25
結果 ------------------------------------------------------- 30
討論 ------------------------------------------------------- 32
參考文獻 ---------------------------------------------------------------- 36
附圖 ---------------------------------------------------------------- 42
附表 ---------------------------------------------------------------- 54
附錄一 ---------------------------------------------------------------- 55
附錄二 ---------------------------------------------------------------- 57
Bachere, E., E. Mialhe, J. Rodriguez. 1995. Identification of defence effectors in the haemolymph of crustaceans with particular reference to the shrimp P. japonicus (Bate): prospects and applications. Fish and Shellfish Immunology 5, 597-612.
Bonami, J. R., K. W. Hasson, J. Mari, B. T. Poulos, D. V. Lightner. 1997. Taura syndrome of marine penaeid shrimp: characterization of the viral agent. Journal of General Wirology 78, 313-319.
Boone, W. R., E. Schoffeniels. 1979. Hemocyanin synthesis during hypoosmotic stress in the shore crab Carcinus maenas (L.). Comparative Biochemistry and Physiology 63B, 207-214.
Brock, J. A., R. Gose, D. V. Lightner, K. W. Jasson. 1995. An overview on Taura syndrome, an important disease of farmed Penaeus vannamei. In: Swimming Through Troubled Water, Proceedings of the Special Session on Shrimp Farming, Aquaculture ’95 eds Browdy, C. L.& J. S. Hopkins pp. 84-89. Baton Rouge, LA: World Aquaculture Society.
Brock, J. A. 1997. Special topic review: Taura syndrome, a disease important to shrimp farms in the Americas. World Journal of Microbiology Biotechnology 13, 415-418.
Castille Jr, F. L., A. L. Lawrence. 1981. The effect of salinity on the osmotic, sodium and chloride concentrations in the hemolymph of euryhaline shrimp of the genus Penaeus. Comparative Biochemistry and Physiology 68A, 75-80.
Chen, J. C., S. Y. Cheng. 1993. Hemolymph osmolality, acid-base balance and shift of ammonotelic to ureotelic excretory pattern of Penaeus japonicus exposed to ambient ammonia. Comparative biochemistry and physiology 106C, 733-737.
Chen, J. C., C. T. Chen. 1996. Changes of osmotic and electrolyte concentrations in the haemolymph of Penaeus japonicus exposed to ambient ammonia. Comparative Biochemistry and Physiology 114C, 35-38.
Chen, Y. L., S. H. Huang, J. H. Cheng, I. H. Tsai. 1993. Relationship between hemolymph coagulation and disease in shrimps: (II) Purification and characterization of the hemolymph coagulogen of penaeids, In: COA Fisheries Series, No. 40 eds. Kou, K. H. Fish Disease Res. 13, 1-9. Taipei, ROC.
Dall, W. 1974. Indices of nutritional state in the western rock lobster Panulirus longipes (Milne Edward)-I. Blood and tissue constituents and water content. Journal of Experimental Marine Biology and Ecology 16, 167-180.
Destoumieux, D., P. Bulet, D. Loew, A. V. Dorsselaer, J. Rodriguez, E. Bachere. 1997. Penaeidins, a new family of antimicrobial peptides isolated from the shrimp Penaeus vannamei (Decapoda). The Journal of Biological Chemistry 272, 28398-28406.
Destoumieux, D., M. Munoz, C. Cosseau, J. Rodriguez, P. Bulet, M. Comps, E. Bachere. 2000. Penaeidins, antimicrobial peptides with chitin-binding activity, are produced and stored in shrimp granulocytes and released after microbial challenge. Journal of Cell Science 113, 461-469.
Djangman, J. S. 1970. The effects of feeding and starvation on copper in the blood and hepatopancreas, and on blood proteins of Crangon vulgaris (Fabricius). Comparative Biochemistry and Physiology 32C, 709-731.
Fuller, G. M., R. F. Doolittle. 1971. Studies of invertebrate fibrinogen. I. Purification and characterization of fibrinogen from the spiny lobster. Biochemistry 10, 1305-1311.
Garza, J. R., K. W. Hasson, B. T. Poulos, R. M. Redman, B. L. White, D. V. Lightner. 1997. Demonstration of infectious Taura syndrome virus in the feces of seagulls collected during an epizootic in Texas. Journal of Aquatic Animal Health 9, 156-159.
Gilles, R. 1977. Effects of osmotic stresses on the protein concentration and pattern of Eriocheir sinensis blood. Comparative Biochemistry and Physiology 56A, 109-114.
Hall, M., M. C. van Heusden, K. Soderhall. 1995. Identification of the major lipoproteins in crayfish hemolymph as proteins involved in immune recognition and clotting. Biochemical and Biophysical Research Communications 216, 939-946.
Hasson, K. W., J. Hasson, H. Aubert, R. M. Redman, D. V. Lightner. 1997. A new RNA-friendly fixative for the preservation of penaeid shrimp sample for virological detection using cDNA genomic probes. Journal of Virological methods 66, 227-236.
Hasson, K. W., D. V. Lightner, L. L. Mohney, R. M. Redman, B. T. Poulos, B. M. White. 1999. Taura syndrome virus (TSV) lesion development and the disease cycle in the Pacific white shrimp Penaeus vannamei. Disease of Aquatic Organisms 36, 81-93.
Hasson, K.W., D. V. Lightner, B. T. Poulos, R. M. Redman, B. L. White, J. A. Brock, J. R. Bonamei 1995. Taura syndrome in Penaeus vannamei: demonstration of a viral etiology. Disease of Aquatic Organism 23, 115-126.
Hennig, O., T. Itami, M. Maeda, M. Kondo, Y. Natsukari, Y. Takahashi. 1998. Analyses of hemolymph immunoparameters in kuruma shrimp infected with penaeid rod-shaped DNA virus. Fish Pathology. 33, 389-393.
Hose, J. E., G. G. Martin. 1989. Defense functions of granulocytes in the ridgeback prawn Sicyonic ingentis. Journal of Invertebrate Pathology 53, 335-346.
Kim, Y. J., W. C. Choi, H. R. Kim, S. J. Jung, M. J. Oh 1999. Changes in Penaeus chinensis haemocytes during white spot baculovirus (WSBV) infections. Bulletin European. Association of Fish Pathologists, 19 (5), 213-215.
Komatsu, M., S. Ando. 1998. A very-high-density lipoprotein with clotting ability from hemolymph of sand crayfish, Ibacus ciliatus. Bioscience, Biotechnology, and Biochemistry 62, 459-463.
Lightner, D. V. 1988. Bacterial shell (brown spot) disease. In: Disease Diagnosis and Control in North American Marine Aquaculture. eds. Sindermann, C. J. and D. V. Lightner. Developments in Aquaculture and Fisheries Science, Vol. 17, pp.48-51. New York, Elsevier.
Lightner, D. V. 1995. Taura syndrome: an economically important viral disease impacting the shrimp farming industries of the Americas including the United States. In: Proceedings of the Ninety-ninth Annual Meeting USAHA, Reno, Nevada. pp. 36-52. Richmond, VA: Pat Campbell & Associates.
Lightner, D. V., R. M. Redman. 1998. Strategies for the control of viral diseases of shrimp in the Americas. Fish Pathology 33, 165-180.
Lotz, J. M. 1997. Effect of host size on virulence of Taura virus to the marine shrimp Penaeus vannamei (Crustacea: Penaeidae). Disease of Aquatic Organism 30, 45-51.
Mari, J., J. R. Bonami, D. V. Lightner. 1998. Taura syndrome of penaeid shrimp: cloning of viral genome fragments and development of specific gene probes. Disease of Aquatic Organisms 33, 11-17.
Martin, G. G., J. E. Hose, S. Omori, C. Chong, T. Hoodbhoy, N. McKrell. 1991. Localization and roles of coagulogen and transglutaminase in hemolymph coagulation in decapod crustaceans. Comparative Biochemistry and Physiology 100B, 517-522.
Medzhitov, R., C. A. Janeway Jr. 1997 Innate immunity: The virtues of a non-clonal system of recognition. Cell 91, 295-298.
Montano-Perez, K., G. Yepiz-Plascencia, I. Higuera-Ciapara, F. Vargas-Albores. 1999. Purification and characterization of the clotting protein from the white shrimp Penaeus vannamei. Comparative Biochemistry and Physiology 122B, 381-387.
Noga, E. J. T. A. Arroll, R. A. Bullis, L. Khoo. 1996. Antibacterial activity in hemolymph of white shrimp, Penaeus setiferus. Journal of Marine Biotechnology 4, 181-184.
Noga, E. J., D. P. Engel, T. W. Arroll, S. McKenna, M. Davidian. 1994. Low serum antibacterial activity coincides with increased prevalence of shell disease in blue crabs Callinectes sapodus. Diseases of Aquatic Organisms 19, 121-128.
Nunan, L. M., B. T. Poulos, D. V. Lightner. 1998. Reverse transcription polymerase chain reaction (RT-PCR) used for the detection of Taura syndrome virus (TSV) in experimentally infected shrimp. Disease of Aquatic Organisms 34, 87-91.
Omori, S. A., G. G. Martin, J. E. Hose. 1989. Morphology of hemocyte lysis and clotting in the ridgeback prawn, Sicyonia ingentis. Cell Tissue Research 255, 117-123.
Overstreet, R. M., D. V. Lightner, K. W. Hasson, S. McLiwain, J. M. Lotz. 1997. Susceptibility to Taura syndrome virus of some penaeid shrimp species native to the gulf of Mexico and the southeastern United States. Journal of Invertebrate Pathology 69, 165-176.
Perazzolo, L. M., M. A. Barracco. 1997. The prophenoloxidase activating system of the shrimp Penaeus paulensis and associated factors. Developmental and Comparative Immunology 21, 385-395.
Perez-Farfante, I., B. F. Kensley. 1997. Penaeoid and sergestoid shrimp and prawns of the world: keys and diagnoses for the families and genera. Memoires Du Museum National D’Histoire naturelle, Tome 175, 1-233.
Renwrantz, L. 1986. Lectins in molluscs and arthopods: their occurrence, origin and roles in immunity. Symposia of the Zoological Society of London 56, 81-93.
Roberson, B. S. 1990. Bacterial agglutination. In: Techniques in Fish Immunology, eds Stolen, J. S., T. C. Fletcher D. P. Anderson, B. S. Roberson & W. B. van Muiswinkel. pp. 81-86. Fair Haven, USA.: SOS Publications.
Rtal, A., L. Nonnotte, J. P. Truchot. 1996. Detoxification of exogenous copper by binding to hemolymph proteins in the shore crab, Carcinus maenas. Aquatic Toxicology 36, 239-252.
Sartoris, F. J., H O. Portner. 1997. Increased concentrations of haemolmph Mg2+ protect intracellular pH and ATP levels during temperature stress and anoxia in the common shrimp Crangon crangon. The Journal of Experimental Biology 220, 785-792.
Sellos, D., S. Lemoine, A. V. Wormhoudt. 1997. Molecular cloning of hemocyanin cDNA from Penaeus vannamei (crustacea, decapoda): structure, evolution and physiological aspects. FEBS Letters 407, 153-158.
Soderhall, K., L. Cerenius. 1992. Crustacean immunity. Annual Review of Fish Diseases 1, 3-23.
Stern, S. 1995. Swimming through troubled waters in shrimp farming: Ecuador Country Review. In: Swimming Through Troubled Water, Proceedings of the Special Session on Shrimp Farming, Aquaculture ’95 eds Browdy, C. L.& J. S. Hopkins pp. 84-89. Baton Rouge, LA: World Aquaculture Society.
Stewart, J. E., J. W. Cornick, J. R. Dingle. 1967. An electronic method for counting lobster (Homarus americanus Milne Edwards) hemocytes and the influence of diet on hemocyte numbers and hemolymph proteins. Canadian Journal of Zoology 45, 291-304.
Tait, J. 1991. Types of crustacean blood coagulation. Journal of the Marine Biological Association of the United Kingdom 9, 191-198.
Tu, C., H. T. Huang, S. H. Chuang, J. P. Hsu, S. T. Kuo, N. J. Li, T. L. Hsu, M. C. Li, S. Y. Lin. 1999. Taura syndrome in Pacific white shrimp Penaeus vannamei cultured in Taiwan. Diseases of Aquatic Organisms 38, 159-161.
Vargas-Albores, F., M. A. Guzman, J. L. Ochoa. 1993. A lipopoysaccharide-binding agglutinin isolated from brown shrimp (Penaeus californiensis Holmes) haemolymph. Comparative Biochemistry and Physiology 104B, 407-413.
Vijayan, K. K., A. D. Diwan. 1996. Fluctuations in Ca, Mg and P levels in the hemolymph, muscle, midgut gland and exoskeleton during the moult cycle of the indian white prawn, Penaeus indicus (Decapoda: Penaeidae). Comparative Biochemistry and Physiology 114A, 91-97.
Wheatly, M. G. 1997. Crustacean models for studying calcium transport: The journey from whole organisms to molecular mechanisms. Journal of the Marine Biological Association of the United Kingdom 77, 107-125.
Yeh, M. S., Y. L. Chen, I. H. Tsai 1998. The hemolymph clottable proteins of tiger shrimp, Penaeus monodon, and related species. Comparative Biochemistry and Physiology 121, 169-176.
Yu, C. I., Y. L. Song. 2000. Outbreaks of Taura syndrome in pacific white shrimp Penaeus vannamei cultured in Taiwan. Fish Pathology 35, 21-24.
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
1. 26.孫本初、陳衍宏(2000)。「政府再造的利器-『標竿學習』理念基礎之介紹」,立法院院聞 28(3):30-39。
2. 42.劉祥得(2000),「『標竿學習法』之研究-政府再造的新策略」,中國行政評論 9(2):91-134。
3. 41.劉宗其,李勝祥(1998),「企業文化與組織氣候對市場資訊處理及績效之影響」,企業管理學報,第43期,pp.167-204。
4. 25.施振榮(1996),「什麼才是競爭力」,天下雜誌,187期,1996年12月,pp.152-159。
5. 23.洪春吉(1999),「台灣地區中、美、日資企業主管之企業文化與企業倫理、領導行為之實證比較」,交大管理學報,第19卷第1期,p.93。
6. 16.林瑞唐(1998),「企業的最佳典範」,能力雜誌,中國生產力中心,1998年9月,P.18-23。
7. 12.吳怡靜,「前瞻,創新,深心知識-一九九八標竿企業」,天下雜誌,民國87年11月,頁66。
8. 30.陳依蘋(2000),專訪勤業管顧總經理顏漏有談企業購併,會計研究月刊,173期,pp.18-22。
9. 29.陳正男,黃文宏(1996),「市場導向之企業文化與根源的探討」,輔仁管理評論,第四卷,第一期,pp.79-110。
10. 49.顏漏有(1997),「全球企業競爭標竿專題報導-企業標竿管理觀念與執行實務」,能力雜誌,中國生產力中心,pp.19-23。
11. 45.廖洲棚(1997),「團隊型組織理論之初採」,人事管理,34-7卷,pp.11 — 16。
12. 1.丁虹,司徒達賢,吳靜吉(1988),「企業文化與組織承諾之關係研究」管理評論,pp.173-198。