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研究生:成寧
研究生(外文):Ning Cheng
論文名稱:台灣鐵杉之組織培養
論文名稱(外文):Tissue Culture of Tsuga chinensis var. formosana
指導教授:王亞男王亞男引用關係
指導教授(外文):Prof. Ya-Nan Wang
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:森林學研究所
學門:農業科學學門
學類:林業學類
論文種類:學術論文
論文出版年:2000
畢業學年度:88
語文別:中文
論文頁數:58
中文關鍵詞:台灣鐵杉組織培養MS培養基SH培養基不定芽癒合組織BATDZ
外文關鍵詞:Tsuga chinensis var. formosanatissue cultureMSSHadventitious budscallusBATDZ
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本試驗以臺灣鐵杉(Tsuga chinensis (Franchet) Pritz. ex Diels var. formosana (Hayata) Li & Keng)之成熟胚為培植體進行培養。將成熟種子先以流水處理48小時,再以70%酒精浸泡兩分鐘,最後在5% NaOCl水溶液(含約1% (v/v) Tween20展著劑)中超音波震盪20分鐘,可達零污染率。取成熟胚進行培養,在添加0.1% (v/v) EM-X溶液之培養基培養可大幅提高發芽率。以MS為基礎培養基在黑暗的環境下,添加1ppm BA及2ppm 2,4-D培養,可獲得大量淡褐色、鬆軟之癒合組織。以MS培養基在光照環境下,添加0.1ppm TDZ培養可促進幼苗生長。以SH培養基培養,無論添加BA、TDZ或BA與2,4-D之組合,皆可獲得不定芽。在黑暗的環境下,BA與2,4-D則可誘導出白色堅硬的癒合組織。將具不定芽之培植體移往不含生長調節劑1/2 SH培養基,以含活性炭與不含活性炭的培養基交互培養,可得小植株。
Results of mature embryo culture of Tsuga chinensis (Franchet) Pritz. ex Diels var. formosana (Hayata) Li & Keng were as follows: mature seeds were first treated with running water for 48 hours, then soaked in 70% ethanol solution for 2 minutes. Soaked in 5%NaOCl (supplemented with 1% (v/v) Tween 20) and treated with ultrasonic shaker for 20 minutes. The medium containing 0.1% (v/v) EM-X could promote germination ratio of mature embryos. Light brown and soft calli were induced after mature embryos were cultured on MS medium containing 1 ppm BA and 2 ppm 2,4-D under dark condition. Mature embryos grew better in MS medium containing 0.1ppm TDZ under light condition. Adventitious buds formed on SH medium containing BA or TDZ or BA with 2,4-D. Under dark condition, white and compact calli were induced in SH medium containing BA and 2,4-D. After the adventitious buds were transferred to 1/2 strength SH medium containing 0.1%(w/v) activated charcoal, and then transferred to 1/2 SH medium with out activated charcoal alternatively, adventitious buds could grow to plantlets.
壹、前言 1
貳、前人研究 2
一、 組織培養途徑 2
二、 影響培植體生長與分化之因子 12
參、材料與方法 15
一、 材料 15
二、 方法 15
肆、結果 18
一、 培植體之表面消毒 18
二、 基礎培養基 18
三、 不同植物生長調節劑的濃度與組合 19
四、 癒合組織的培養 31
五、芽體的抽長及誘根 32
六、細懸浮培養 33
伍、結論 34
陸、參考文獻 36
柒、附錄 45
捌、照片 47
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