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研究生:翁茂倫
論文名稱:臺灣產深山粉背蕨複合種群之研究
論文名稱(外文):The study of Cheilanthes farinosa(Forsk.)kaulf. species complex in Taiwan
指導教授:郭城孟郭城孟引用關係
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:植物學研究所
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2000
畢業學年度:88
語文別:中文
論文頁數:55
中文關鍵詞:臺灣深山粉背蕨複合種群
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中文摘要
深山粉背蕨Cheilanthes farinosa (Forsk.) Kaulf.是屬於中小型的蕨類植物,主要分布在世界熱帶至溫帶地區的乾燥岩生環境,由於具有相當程度的形態差異,因此學者認為是分類關係有待釐清的複合種群,臺灣產深山粉背蕨複合種群{Cheilanthes farinosa (Forsk.) Kaulf. species complex}也不例外,過往關於此複合種群的分類學研究曾經使用過七個不同的學名,其中包含3個不同的分類群,顯示彼此之間具有一定程度的歧異性,因此本研究採用以下的方法以了解複合種群之間的親緣關係,並對分類處理提出建議。(1)外部形態特徵觀察,包括葉柄鱗片與葉片形態(2)孢子囊內孢子數目計算以及(3)核醣體DNA中的ITS(internal transtribed spacer)序列資料,以Kimura 2-parameter distance計算遺傳距離後,使用MEGA軟體的非加權配對群組(UPGMA)以及近鄰群組(Neighbor-Joining)的計算方式架構親緣關係樹。本研究以11個不同採集地點的深山粉背蕨複合種群的植株為研究材料,並以長柄粉背蕨Cheilanthes argentea(Gmel.)Kunze作為外群。結果顯示,此複合種群的葉柄鱗片具有兩類非連續性的形態差異,其一為中央黑色不透明邊緣褐色的雙色鱗片,其二為單色褐色鱗片,葉形分為兩類,分別為長橢圓形與披針形,此形態特徵的區分與ITS親緣關係樹的分支相符合,並且彼此間的遺傳距離大於與外群的距離,因此適合處理為不同種類,而葉柄鱗片形態、葉形變化可為鑑定特徵,另外,由ITS序列資料顯示分布於玉山塔塔加地區的族群可能為雜交族群,但仍需進一步的研究。研究材料的孢子數目均為64個,顯示並無無配生殖現象。

Abstract
Cheilanthes farinosa (Forsk.) Kaulf. is widely distributed in dry and rocky place of tropical and temperate region of the world. Its size is moderate to small. Because high diversity of morphology among this species, pteridologist pointed out this is a species complex and further taxonomic study is needed. There is also high diversity of the species complex in Taiwan. In the former taxonomic study, seven different scientific names have been applied. This study is to understand the phylogenetic relationship of Cheilanthes farinosa (Forsk.) Kaulf. species complex. 11 different populations from different locations are sampled and Cheilanthes argentea (Gmel.) Kunze is used to be outgroup. Morphological characters are examined, including the scale of stipe and the morphology of frond. Spore number of a sporangium is also counted. And Using the ITS of rDNA sequence data, Kimura 2-parameter distance is counted and phylogenetic tree are obtained based on UPGMA and Neighbor-Joining by MEGA package. There are two discontinuous types of scales of stipe, one is discolor and margin brown with a black line in the middle, the other is concolor brown. There are two different type of the morphology of the frond, one is oblong and the other is lanceolate. The morphological character and phylogenetic tree topologies are matched and indicated that this species complex includes two species by their longer genetic distance than the outgroup. And according to the ITS sequence, there is maybe a hybrid population in Tataka of Yu-Shan National Park. The spore number per sporangium of all samples are 64, indicate there is not apogamy among them.

目錄
中文摘要…………………………………………………………..i
英文摘要…………………………………………………………..iii
壹、 前言…………………………………………………………..1
貳、 材料與方法…………………………………………………..10
參、 結果…………………………………………………………..21
肆、 討論…………………………………………………………..32
伍、 分類處理……………………………………………………..39
陸、 結論…………………………………………………………..41
柒、 參考文獻……………………………………………………..42
附錄………………………………………………………………..49
表目次
表1、歷年臺灣產深山粉背蕨複合種群之分類處理……….…..5
表2、台灣產深山粉背蕨複合種群植物材料之採集地點………10
表3、ITS序列分析所使用的引子……………………………….15
表4、葉柄鱗片形態………………………………………………21
表5、葉片形態…………………………………………………….23
表6、每個孢子囊內孢子數目統計……………………………….24
表7、各OTU之ITS序列長度……………………………………27
表8、以Kimura 2-parameter計算之遺傳距離……………..…….29
圖目次
圖1、台灣產深山粉背蕨複合種群植物材料之採集地點……….11
圖2、使用UPGMA所架構的親緣關係樹……………………….30
圖3、使用Neighbor-Joining所架構的親緣關係樹………………31

柒、參考文獻
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間接引用
Fabbri, F. 1965. Secondo supplementa alle Tavole Cromosomiche delle pteridophyta de Alberto Chiarugi. Caryologia 18:675-731. Cited in Knobloch, I. W. 1966. A preliminary review of spore number and apogamy within the genus Cheilanthes. Amer. Fern J. 56:163-167.
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