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研究生:顏淑雲
研究生(外文):Shu-Yun Yen
論文名稱:波紋硬囊實果菌(Sclerocystissinuosa)種內遺傳與繁殖之多樣性研究
論文名稱(外文):The diversity of intraspecific genetics and reproduction of Sclerocystis sinuosa (Glomales)
指導教授:陳瑞青陳瑞青引用關係吳繼光
指導教授(外文):Zuei-Ching ChenChi-Guang Wu
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:植物學研究所
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2000
畢業學年度:88
語文別:中文
論文頁數:64
中文關鍵詞:囊叢枝菌根菌繡球孢子目波紋硬囊實果菌18S 核糖體DNA孢子果
外文關鍵詞:vesicular-arbuscular mycorrhizal fungiGlomalesSclerocystis sinuosa18S ribosomal DNAsporocarp
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囊叢枝菌根菌之分類主要依其產生之無性孢子形態,因此常有混淆。台灣產波紋硬囊實果菌Sclerocystis sinuosa會產生兩種孢子果,兩種孢子果主要不同點在於包被的有無,因兩種之其它外型差異不大,引起此兩種孢子果是否為同種之懷疑。本論文則針對此菌種之生物多樣性,藉由掃描式電子顯微鏡、形態連續觀察及18S ribosomal DNA 序列比較等方法來研究,試求來解決此問題。
在以砂耕法多孢接種後,確實發現純種菌株能產生兩種孢子果,且兩種孢子果的所佔比例會隨時間消長;若接種本菌株中無包被的孢子果較易產生兩型孢子果,而接種有包被之孢子果則產生無包被孢子果的比例較低。在繁殖菌種時也發現似乎是兩種孢子果的中間型,經掃瞄式電子顯微鏡觀察發現裸露孢子果被菌絲逐漸包裹,而形成包被。將本菌株無包被與有包被兩型孢子果之18S ribosomal DNA 序列比對,發現此兩種不同表型之菌株DNA序列相同;再與其他已發表之菌種比對,與 Glomus sinuosum 具最高相似性,但仍有不同的鹼基。故認為會產生兩型孢子果之台灣產S. sinuosa與G. sinuosum系屬同一種,但與國外已發表之菌株為不同品系。
Arbuscular mycorrhizal fungi (AMF) are one of the most widespread group of fungi on earth. Taxonomists have described three families, six genera and approx. 150 species. This taxonomy is based on morphological characters, and especially on features of asexual spores. These characteristics may be difficult to discern and are subjected to alterations during spore ontogeny or by parasitism. And the morphological similarity might conceal physiological diversity in these fungi.
There are two kinds of sporocarp of Sclerocystis sinuosa collected from Taiwan. The size and shape of two sporocarp types are similar; however, they differ in the presence or lacking of sporocarp peridium. The present investigation employed 18S ribosomal DNA sequences, sporocarp ontogeny and pot inoculation test to explore their identity.
Numbers and ratios of sporocarp with different mode of peridium formation were varied according to time change after inoculation either by naked sporocarps or sporocarps with peridium. Two types of sporocarps were reproduced at maturity by inoculating solely naked sporocarps in root of sorghum seedlings in the sand pot culture. In contrast, only sporocarps covered peridium were reproduced solely at maturity by inoculating with peridial sporocarps in the same host and environment.
As the result of 18S ribosomal DNA sequence, there is no difference between two sporocarp types. But slight variation in sequence compared with Glomus sinuosum were observed. The two sporocarp types of S. sinuosa collected from Taiwan are thus identified as the same species with G. sinuosum, but can be treated as a different strai
目錄
誌謝
目錄 Ⅰ
圖表目錄 Ⅱ
中文摘要 Ⅵ
英文摘要 Ⅶ
壹、前言 1
貳、材料與方法
一、材料 7
二、形態觀察 9
三、孢子果比重測試 10
四、18S ribosomal DNA 序列分析 11
參、結果
一、孢子果數連續採樣統計 14
二、孢子果的發育觀察 16
三、孢子果比重測試 17
四、18S ribosomal DNA序列分析 17
肆、討論
一、孢子果數統計 18
二、孢子果發育過程 19
三、孢子果的變異 20
四、孢子果比重測試 20
五、18S ribosomal DNA序列分析之可靠性 24
伍、結論 25
陸、參考文獻 26
圖表 31
附錄 63
Almeida, R. T., and N. C. Schenck. 1990. A revision of the genus Sclerocysis (Glomaceae, Glomales). Mycologia 82: 703-714.
Ames, R. N., and R. W. Schneider. 1979. Entrophospora, a new genus in the Endogonaceae. Mycotaxon 8: 347-352.
Becard, G., and P. E. Pfeffer, 1993. Status of nuclear division in arbuscular mycorrhizal fungi during in vitro development. Protoplasma 194: 62-68.
Benjamin, R. K. 1979. Zygomycetes and their spores. In: The whole fungus.Ⅱ. B. Kendrick (ed.). National Museums of Canada, Ottawa. 573-722.
Berkeley, M. J., and C. E. Broome. 1873. Enumeration of the fungi of Ceylon.Ⅱ. J. Linn. Soc. London, Bot. 14: 137.
Bruns, T. D., T. J. White, and T. W. Taylor. 1991. Fungal molecular systematics. Annu. Rev. Ecol. Syst. 22: 525-564.
Daniels, B. A., and H. D. Skipper. 1982. Methods for the recovery and quantitative estimation of progagules from soil. In: Methods and principles of mycorrhizal research. N. C. Schenck (ed.). The American Phytopathological Society, St. Paul. 20-45.
Danielson, R. M. 1982. Taxonomic affinities and criteria for identification of the common ectomycorrhizal symbiont of pines. Canada. J. Bot. 60: 7-18.
Dodd, J. C., S. Giovannetti, M. Broome, A. Lanfranco, and C. Walker. 1996. Inter- and intraspecific variation within the morphologically similar arbuscular mycorrhizal fungi Glomus mosseae and Glomus coronatum. New Phytol. 133: 113- 122.
Frank, A. B. 1885. Uber die auf Wurzelsymbiose beruhende Ernahrung gewisser Baume durch unterirsische Pilze. Ber. Deut. Bot. Gesell. 3: 128-145.
Gallaud, I. 1905. Etudes sur les mycorrhizes endotrophs. Revue Generdle de Botanique 17: 5-48, 66-83.
Gerdemann, J. W., and J. M. Trappe. 1974. The Endogonaceae in the Pacific Northwest. Mycologia Memoir 5: 76.
Gerdemann, J. W., and B. K. Bakshi. 1976. Endogonaceae of India: two new species. Trans. Brit. Mycol. Soc. 66: 340-343.
Gibson, J. L. 1986. Morphology, cytoloty and ultrastructure of selected species of Endogonaceae (Endogonales: Zygomycetes). Ph. D. Dissertation, University of Florida, Gainesville, Florda. 166.
Giovannetti, M., and V. Gianinazzi-Pearson. 1994. Biodiversity in arbuscular mycorrhizal fungi. Mycol. Res. 98: 705-715.
Hawksworth, D. L., P. M. Kirk, B. C. Satton, and D. N. Pegler (eds.). 1995. Dictionary of the fungi. Eighth edition. University Press, Cambridge. 437.
Koske, R. E., and B. Tessier. 1983. A convenient, permanent slide mounting medium. Newsletter Mycol. Soc. Amer. 34: 59.
Koske, R. E., and J. N. Gemma. 1989. A modified procedure staining roots to detect VA mycorrhizas. Mycol. Res. 92: 486-488.
Lin, Su-Chen. 1998. Ecological study of vesicular- arbuscular mycorrhizal fungi and its application in Taiwan. Doctoral Dissertation, National Taiwan University.
Lloyd-Macgilp, S. M. Chambers, J. C. Dodd, A. H. Fitter, C. Walker, and J. P. W. Young. 1996. Diversity of the ribosomal internal transcribed spacers within and among isolates of Glomus mosseae and related mycorrhizal fungi. New Phytol. 133: 103-111.
Mitchell, J. I., P. J. Roberts, and S. T. Moss. 1995. Sequence or structure? A short review on the application of nucleic acid sequence information to fungal taxonomy. Mycologist 9: 67-75.
Moreau, F. 1953. Les champignons, TomeⅡ. Systematique. Encycl. Mycol. 23: 1-1231.
Morton, J. B. 1990. Evolutionary relationships among arbuscular mycorrhizal fungi in the Endogonaceae. Mycologia 82: 192-207.
Morton J.B., and G. L. Benny. 1990. Revised classification of arbuscular mycorrhizal fungi (Zygomycetes): A new order, Glomales, two new suborders, Glominae and Gigasporineae, and two new families, Acaulosporaceae and Gigasporaceae, with an emendation of Glomaceae. Mycotaxon 37: 471-491.
Morton, J. B., J. D. Bever, and F. L. Pfleger. 1997. Taxonomy of Acaulospora gerdemannii, and Glomus leptotichum, synanamorphs of an arbuscular mycorrhizal fungus. Mycol. Res. 101: 625-631.
Pirozynski, K. A., and Y. Dalpe. 1989. Geological history of the Glomaceae with particular reference to mycorrhizal symbiosis. Symbiosis 7: 1-36.
Redecker, D., H. Thierfekder, C. Walker, and D. Werner. 1997. Restriction analysis of ribosomal DNA as a tool for species identification in different fenera of the order Glomales. Appl. Environ. Microbiol. 63: 1756-1761.
Redecker, D., J. B. Morton, and T. D. Bruns. 2000. Molecular phylogeny of the arbuscular mycorrhizal fungi Glomus sinuosum and Sclerocystis coremioides. Mycologia 92: 282-285.
Sanders I. R., M. Alt, K. Groppe, T. Boller, and A. Wiemken. 1995. Identification of ribosomal DNA polymorphisms among and within spores of the Glomales: application to studies on the genetic diversity of arbuscular mycorrhizal fungal communities. New Phytol. 130: 419-427.
Sawaki, H., K. Sugawara, and M. Saito. 1998. Phylogenetic position of an arbuscular mycorrhizal fungus, Acaulospora gerdemannii, and its synanamorph Glomus leptotichum, based upon 18S rRNA gene sequence. Mycoscience 39: 477-480.
Scannerini S., and Bonfante P. 1991. Bacteria and bacteria- like objects in endomycorrhizal fungi (Glomaceae). In: Symbiosis as a source of evolutionary innovation. L. Margulis, and R. Fester (eds.). MIT Press, Cambridge. 273-287.
Schenck, N. C., and Y. Perez (eds.). 1990. Manual for the identification of VA mycorrhizal fungi. Third edition. Synergistis Publications.
Simon L., M. Lalonde, and T. D. Bruns. 1992. Specific amplification of 18S fungal ribosomal genes from vesicular-arbuscular endomycorrhizal fungi colonizing roots. Appl. Environ. Microbiol. 58: 291-295.
Simon L., J. Bousquet, R. C. Levesque, and M. Lalonde. 1993. Origin and diversification of endomycorrhizal fungi and coincidence with vascular land plants. Nature 363: 67-69.
Smith, G. W., and N. C. Schenck. 1985. Two new dimorphic species in the Endogonaceae: Glomus ambisporum and Glomus heterosporum. Mycologia 77: 566-574.
Simth, S. E., and D. J. Read (eds.). 1997. Mycorrhizal symbiosis. Twice edition. Academic Press, San Diego. 9-126.
Susan, I.(ed.). 1992. Mutualistic symbioses. In: Fungal-plant interaction. Chapman & Hall Published. London. 266-285.
Trappe, J. M., and N. C. Schenck. 1982. Taxonomy of the fungi forming endomycorrhizal fungi (Endogonales). In: Methods and principles of mycorrhizal research. N. C. Schenck (ed.). Am. Phytopath. Soc., St. Paul, Minnesota. 1-9.
Vandenkoornhuyse, P., and C. Leyval. 1998. SSU rDNA sequencing and PCR-fingerprinting reveal genetic variation within Glomus mosseae. Mycologia 90: 791-797.
Walker, C. 1979. Complexipes moniliformis: a new genus and species tentatively placed in the Endogonaceae. Mycotaxon 12: 99-104.
Walker, C., and F. E. Sanders. 1986. Taxonomic concepts in the Endogonaceae: Ⅲ. The separation of Scutellospora gen. nov. from Gigaspora Gerd. & Trappe. Mycotaxon 27: 169-182.
Walker, C. 1987. Current concepts in the taxonomy of the Endogonaceae. In: Mycorrhizae in the next decade, practical applications and research priorities. D. M. Sylvia, L. L. Hung, and J. H. Graham (eds.). IFAS. University of Florida, Gainesville. 300-302.
White, T. J., T. Bruns, S. Lee, J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: A guide to methods and applications. M. A. Innis, D. H. Gelfand, J. J. Sninsky, and T. J. White (eds.). Academic Press. 315-322.
Wu, C. G. 1983. The preliminary studies on the endomycorrhizae of bamboo forest (Phyllostachys pubscens Mazel. ) at Chi-tou. Master’s thesis, National Taiwan University.
Wu, C. G., and Z. C. Chen. 1986. The endogonaceae of Taiwan.Ⅰ: A preliminary investigation on endogonaceae of bamboo vegetation at Chi-tou areas, Central Taiwan. Taiwania 31: 65-88.
Wu, C. G., and Z. C. Chen. 1987. The endogonaceae of Taiwan.Ⅱ: Two new species of Sclerocystis from Taiwan. Trans. Mycol. Soc. R. O. C. 2: 73-83.
Wu, C. G. 1993a. Glomales of Taiwan: Ⅲ A comparative study of spore ontogeny in Sclerocystis (Glomaceae, Glomales). Mycotaxon 47: 25-39.
Wu, C. G. 1993b. Glomales of Taiwan: Ⅳ A monograph of Sclerocystis (Glomaceae). Mycotaxon 49: 327-349.
Wu, C. G. 1993c. Observation of Sclerocystis sinuosa in Taiwan (Glomaceae, Glomales). Trans. Mycol. Soc. Japan 34: 283-290.
Wu, C. G., and Su-Chen Lin (eds.). 1998. Technical manual of vesicular- arbuscular mycorrhizal fungi. Taiwan Agricultural Research Institute Published.
Zeze, A., E. Sulistyowati, K. Ophel-keller, S. Barker., and S. Smith. 1997. Intersporal genetic variation of Gigaspora margarita, a vesicular arbuscular mycorrhizal fungus, revealed by M13 minisatellite-primed PCR. Appl. Environ. Microbiol. 63: 676-678.
黃玲瓏, 1998。 電子顯微鏡學實驗講義。國立台灣大學植物學研究所。
陳懋彥,黃玉芬,陳榮銳,陳瑞青。 1999。 植物分子分類學實驗手冊。國立台灣大學植物學研究所。
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