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研究生:陳勝利
研究生(外文):Sheng-Lee Chen
論文名稱:水稻幼苗低溫耐性相關基因之選殖
論文名稱(外文):Identification of chilling tolerance-related genes in rice seedlings
指導教授:朱 鈞盧虎生盧虎生引用關係
指導教授(外文):Chun ChuHuu-Sheng Lur
學位類別:博士
校院名稱:國立臺灣大學
系所名稱:農藝學研究所
學門:農業科學學門
學類:一般農業學類
論文種類:學術論文
論文出版年:2000
畢業學年度:88
語文別:中文
論文頁數:167
中文關鍵詞:水稻(Oryza sativa L.)低溫耐性離層酸methyl jasmonate差異表現法電解質滲漏法
外文關鍵詞:rice (Oryza sativa L.)chilling toleranceabscisic acidmethyl jasmonatedifferential displayelectrolyte leakage
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  水稻(Oryza sativa L.)在台灣及其他亞熱帶地區早春的幼苗時期經常遭受冷害,此冷害可能導致作物生長受阻甚至降低產量。為了進一步探究調控冷害的分子機制,本研究以差異表現法(differential display)並配合銀染的技術來選殖與水稻幼苗低溫耐性表現相關的cDNAs。以低溫敏感的印度型水稻台中在來1號(TN 1)及低溫抗性的日本型台農67號(TNG 67)幼苗,經過(1)5℃;(2)25℃(對照組);(3) 10 mM ABA,25℃;或(4)10 mM methyl jasmonate (MeJa),25℃;各24小時處理後,取其根部之total RNA供進行差異表現法試驗。結果由600個差異表現片段中,成功再擴增出362個差異表現之cDNA片段,經基因庫比對結果,發現有153個(42.3 %) cDNA與已知基因有極高的相似性;61個cDNA分別與rab16D、heat shock protein、SalT、Zn-induced protein……等29個已知功能基因有極高的相似性。
另外以RNA slot blot分析結果發現,有58個cDNA clones在低溫或ABA處理下有極高的表現。利用北方墨點分析法(northern-blot analysis)自58個差異表現的片段中,選出28個在低溫、ABA及其它逆境處理下的表現與對照組有明顯差異的cDNA clones。其中有部分cDNA分別在低溫、ABA、MeJa、缺水、鹽害及熱休克(heat shock)處理下的水稻根部或地上部具專一性表現。以11個分別具有不同低溫耐性及ABA內生量的水稻品種,來測試這28個cDNA clones的表現,發現有6個基因在根部或地上部的表現量與電解質滲漏量呈顯著負相關,顯示這6個基因與低溫耐性相關。
低溫逆境下ABA前處理可導致部分低溫耐性基因在低溫敏感的水稻台中在來1號幼苗中大量表現,顯示這些基因可能參與水稻幼苗受ABA調節所產生的低溫耐性。另外,部分基因在未經ABA前處理的水稻幼苗中大量表現,顯然它們的表現不受ABA的影響。本試驗的結果也顯示,差異表現法可有效的選殖水稻幼苗低溫相關基因。
  Rice (Oryza sativa L.) seedlings frequently suffer from chilling injury during early spring in Taiwan as well as in other subtropical areas. The chilling injury may cause severe growth retardation and yield reduction. To gain more insights of molecular mechanisms of chilling tolerance, cDNAs associated with chilling tolerance in rice seedlings were isolated using differential display of mRNA. Seedlings of a chilling-sensitive indica type cultivar (cv. Taichung Native 1, TN 1), and a chilling-tolerant japonica type cultivar (cv. Tainung 67, TNG 67) were treated for 24 h with either: (1) 5oC; (2) 25oC (control); (3) 10-5 mM ABA at 25oC; or (4) 10-5 M methyl jasmonate (MeJa) at 25oC. The later two treatments were previously found that could induce chilling-resistance in TN1 seedlings. Total RNA were isolated from the roots of seedlings and used for differential display. Clones of 362 cDNA were re-amplified and isolated from 600 differentially expressed cDNA fragments. Sequences of 153 cDNA clones had significant similarities to the sequences known in the GenBank. Sixty-one of these clones, which could be separated to 29 groups, showed high homology to the sequences with known functions, i.e., rab16D, heat shock protein, SalT, and Zn-induced protein.
Slot-blot analysis revealed that 58 cDNA clones could be regulated by ABA, MeJA or chilling treatments. Among these 58 differentially expressed cDNA clones, 28 cDNA clones could be enhanced by ABA or chilling using northern blot analysis. In addition, 6 clones of the 28 clones showed differential expressions under stresses including chilling, osmotic, salt, heat shock, and ABA or MeJa treatments. By testing their expressions in 11 cultivars, which have different chilling tolerance and different chilling-induced ABA levels, six cDNA clones showed positively correlated with chilling tolerance. Some of these cDNA clones showed much higher transcript levels after exposure to chilling stress in TN 1 seedlings that pre-treated with ABA. Therefore, these clones might be involved in the ABA-mediated acclimation of chilling resistance in TN 1 seedlings. In contrast, transcript levels of other cDNA clones were up-regulated in seedlings without ABA pre-treatment, and their expressions seemed not to be related to endogenous ABA levels. In the current study, the technique of mRNA differential display was successfully used to identify genes associated with chilling tolerance in rice seedlings.
封面

表目錄
圖目錄
中文摘要
英文摘要
縮寫字
第一章 緒言
第二章前人研究
第三章利用差異表現法選殖水稻幼苗低溫耐性基因及低溫耐性相關基因之表現
第一節 前言
第二節 材料與方法
第三節 結果
第四節 討論
第四章 水稻低溫耐性相關基因與低溫耐性表現之關係
第一節 前言
第二節 材料與方法
第三節 結果
第四節 討論
第五章
參考文獻
附錄
戶義刈次。1963。作物試驗法。東京農業技術學會印行。第159-176頁。
朱德民。1984。植物與環境逆境。國立編譯館印行。第109頁。
李澤民。1991。離層酸及多元在水稻幼苗耐冷性上之意義。國立台灣大學農藝學研究所博士論文。
林茂森。2000。台灣水稻品種之歸群分析。中華農學會報。1: 89-98。
高小玲。1994。離層酸(ABA)與高粱幼苗耐冷性之關係。國立台灣大學農藝學研究所博士論文。
掘末登、国広泰史、東正昭、小三田善山、王懷義、熊建華、張思竹、李智勇、王永華。1988。日中品種の耐冷性と標準品種の選定。Trop Agric Res Ser 21: 76-87。
陳勝利、盧虎生、朱鈞。1997。差異表現法(differential display)在植物基因表現研究上的應用。科學農業45:145-152。
楊邦棋、劉麗飛、盧虎生、朱鈞。1998。離層酸與鈣離子對水稻切離根耐冷性之影響。中華農藝8: 151-160。
劉復誠。1999。台灣地區近年來的最大一次寒害。中華農業氣象學會簡訊 9: 1。
薛亦晴。2000。水稻耐冷性生理性狀之遺傳研究。國立台灣大學農藝學研究所碩士論文。
Adams MD, Dubnick M, Kerlavage AR, Moreno R, Kelley JM, Utterback TR, Nagle JW, Fields C, Venter JC (1992) Sequence identification of 2,375 human brain genes. Nature 355: 632-634
Adams MD, Kelley JM, Gocayne JD, Dubnick M, Polymeropoulos MH, Xiao H, Merril CR, Wu A, Olde B, Moreno RF, Kerlavage AR, McCombie, Venter JC (1991) Complementary DNA sequencing: expressed sequence tags and human genome project. Science 252: 1651-1656
Adnan S, Susan L, Weiss D (1998) Isolation and characterization of a heat-induced gene, hcit2, encoding a novel 16.5 kDa protein: expression coincides with heat-induced tolerance to chilling stress. Plant Mol Biol 36: 935-939
Aguan K, Sugawara K, Suzuki N, Kusano T (1991) Isolation of genes for low-temperature-induced proteins in rice by a simple subtractive method. Plant Cell Physiol 32: 1285-1289
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215: 403-410
An G, Leu G, Veltri RW, O’Hara SM (1996) Sensitive, nonradioactive differential display method using chemiluminescent detection. BioTechniques 20: 342-346
Bauer D, Muller H, Reich J, Riedel H, Arenkiel V, Warthoe P, Strauss M (1993) Identification of differentially expressed mRNA species by an improved display technique (DDRT-PCR). Nucleic Acid Res 21: 4272-4280
Boguski MS (1995) The turning point in genome research. Trends Biochem Sci 20: 295-296
Bouchez D, Hofte H (1998) Functional genomics in plants. Plant Physiol 118: 725-732
Bray EA (1988) Drought-and ABA-induced changes in polypeptide and mRNA accumulation in tomato leaves. Plant Physiol 88: 1210-1214
Burnett WV (1997) Northern blotting of RNA denatured in glyoxal without buffer recirculation. BioTechniques 22: 668-671
Chen JJW, Peck K (1996) Non-radioisotopic differential display method to directly visualize and amplify differential bands on nylon membrane. Nucleic Acid Res 24: 793-794
Chen THH (1994) Plant adaptation to low temperature stress. Canad J Plant Pathol 16: 231-236
Chen THH, Gusta LV (1983) Abscisic acid-induced freezing resistance in cultured plant cells. Plant Physiol 73: 71-75
Choi KH, Choi HS, Lee CH, Kwon YM, Rhew TH (1997) Identification of genes induced by low temperature in rice. J Biochem Mol Biol 30: 292-295
Christiansen MN, Carns HR, Slyter DJ (1970) Stimulation of solute loss from radicales of Gossypium hirsutum L. by chilling anaerobiosis and low pH. Plant Physiol 46: 53-56
Chu C, Hwang SJ, Lee TM (1986) Hormonal regulation of cold-resistance in rice seedlings. In Phytohormones Proc 5th Seminar on Science and Tech. Nara, Japan. Interchanges Assoc, Tokyo, Japan, pp 125-154
Chu C, Lee TM (1989) The relationship between ethylene biosynthesis and chilling tolerance in seedlings of rice (Oryza sativa L.). Bot Bull Acad Sin 30: 263-273
Chu C, Lee TM (1992) Regulation of chilling tolerance in rice seedlings by plant hormones. Korean J Crop Sci 37: 288-298.
Claes B, Dekeyser R, Villarroel R, Van den Bulcke M, Bauw G, Van Montagu M, Caplan A (1990) Characterization of a rice gene showing organ-specific expression in response to salt stress and drought. Plant Cell 2: 19-27
Daie J, Campbell WF (1981) Response of tomato plants to stressful temperatures. Plant Physiol 67: 26-29
Davies WJ, Mansfield TA, Hetherington AM (1990) Sensing of soil water status and the regulation of plant growth and development. Plant Cell Environ 13: 709-719
de Pater BS, van der Mark F, Rueb S, Katagiri F, Chua NH, Schilperoort RA, Hensgens LA (1992) The promoter of the rice gene GOS2 is active in various different monocot tissues and binds rice nuclear factor ASF-1. Plant J 2: 837-844
Doss RP (1996) Differential display without radioactivity--A modified procedure. BioTechniques 21: 408-410
Eamus D (1986) The responses of leaf water potential and leaf diffusive resistance to abscisic acid, water stress and low temperature in Hibiscus esculentus: The effect of water stress and ABA pre-treatments. J Exp Bot 37: 1854-1862
Eamus D, Wilson JM (1983) ABA levels and effects in chilled and hardened Phaeolus vulgaris. J Exp Bot 34: 1000-1006
Forney CF, Peterson SJ (1990) Chilling induced potassium leakage of cultured citrus cells. Physiol Plant 78: 193-196
Frandsen G, Muller-Uri F, Nielsen M, Mundy J Skriver K (1996) Novel plant Ca (2+)-binding protein expressed in response to abscisic acid and osmotic stress. J Biol Chem 271: 343-348
Gau SL, Lur HS, Chu C (1992) Amelioration of sorghum (Sorghum bicolor L.) seedlings to chilling stress by abscisic acid. The Proceedings of the Society for the Advance of Breeding Researches in Asia and Oceanian (SABRO). International Symposium on Impact of Biological Research on Agricultural Productivity. Taichung, Taiwan, R.O.C. pp. 255-264
Genoud T, Metraux JP (1999) Crosstalk in plant cell signaling: structure and function of the genetic network. Trend Plant Sci 4: 503-507
Gilmour SJ, Artus NN, Thomashow MF (1992) cDNA sequence analysis and expression of two cold-regulated genes of Arabidopsis thaliana. Plant Mol Biol 18: 13-21
Gilmour SJ, Zarka DG, Stockinger EJ, Salazar MP, Houghton JM, Thomashow MF (1998) Low temperature regulation of the Arabidopsis CBF family of AP2 transcriptional activators as an early step in cold-induced COR gene expression. Plant J 16: 433-442
Guy CL, Haskell D (1987) Induction of freezing tolerance in spanish is associated with the synthesis of cold acclimation induced proteins. Plant Physiol 84: 872-878
Guy MG (1989) Phospholipid, sterol composition and ethylene production in relation to choline-induced chill-tolerance in mung bean (Vigan radiata L. Wilcz) during a chilling-warm cycle. J Exp Bot 40: 369-374
Hajela RK, Horvath DP, Gilmour SJ, Thomashow MF (1990) Molecular cloning and expression of cor (cold regulated) genes in Arabidopsisis thaliana. Plant Physiol 93: 1246-1252
Hofte H, Desprez T, Amselm J, Chiapello H, Caboche M, Moisan A, Jourdan MF, Charpentou JL, Berthomieu P, Guerrier D, Giraduat J, Quigley F, Thomas F, Yu DY, Mache R, Raynal M, Cooke R, Grellet E, Delseney M, Parmentier Y, Marcillac G, Gigot C, Fleck J, Phillips G, Axelos M, Bardet C, Tremousaygue D, Lescure B (1993) An inventory of 1152 expressed sequenced tags obtained by partial sequencing of cDNA from Arabidopsis thaliana. Plant J 4: 1051-1061
Hughes MA, Dunn MA, Pearce RS, White AJ, Zhang L (1992) An abscisic acid-responsive, low temperature barley gene has homology with a maize phospholipid transfer protein. Plant Cell Environ 15: 861-865
Ito T, Kito K, Adati N, Mitsui Y, Hagiwara H, Sakaki Y (1994) Fluorescent differential display: arbitrarily primed RT-PCR fingerprinting on an automated DNA sequencer. FEBS Letters 351: 231-236
Jaglo-Ottosen KR, Gilmour SJ, Zarka DG, Schabenberger O, Thomathow MF (1998) Arabidopsis CBF1 overexpression induces COR genes and enhances freezing tolerance. Science 280: 104-106
Johnson RR, Cranston HJ, Chaverra ME, Dyer WE (1995) Characterization of cDNA clones for differential expressed genes in embryos of dormant and nondormant Avena fatua L. caryopses. Plant Mol Biol 28: 113-122
Kefu Z, Munns R, King RW (1991) Abscisic acid level in NaCl-treated barley, cotton and salt bush. Aust J Plant Physiol 18: 17-24
Keith CS, Hoang DO, Barret BM, Freigelman B, Nelson MC, Thai H, Baysdorfer C (1993) Partial sequence analysis of 130 randomly selected maize cDNA clones. Plant Physiol 101: 329-332
Koster KL, Lynch DV (1992) Solute accumulation and compartmentation during the acclimation of Puma rye. Plant Physiol 98: 108-113
Kurkela S, Franck M (1990) Cloning and characterization of a cold- and ABA-inducible Arabidopsis gene. Plant Mol Biol 15: 137-144
Laberge S, Caastonguay Y, Vezina LP (1993) New cold-and drought-regulated gene from Medicago sativa. Plant Physiol 101: 1411-1412
Le Tran Binh, Oono K (1989) Ecotypical responses and specific protein pattern of rice seedlings and callus during chilling-treatment. Jap J Breed 39: (suppl. 2) 132-133
Le Tran Binh, Oono K (1992) Molecular cloning and characterization of genes related to chilling tolerance in rice. Plant Physiol 99: 1146-1150
Lee TM (1997) Polyamine regulation of growth and chilling tolerance of rice (Oryza sativa L.) roots cultured in vitro. Plant Sci 122:111-117
Lee TM, Chu C (1992) Ethylene-induced polyamine accumulation in rice (Oryza sativa L.) coleoptiles. Plant Physiol 100: 238-245
Lee TM, Lur HS, Chu C (1993) Role of abscisic acid in chilling tolerance of rice (Oryza sativa L.) seedlings. Ⅰ. endogenous abscisic acid levels. Plant Cell Environ 16: 481-490
Lee TM, Lur HS, Chu C (1995) Abscisic acid and putrescine accumulation in chilling-tolerant rice cultivars. Crop Sci 35: 502-508
Lee TM, Lur HS, Chu C (1997) Role of abscisic acid in chilling tolerance of rice (Oryza sativa L.) seedlings. II. Modulation of free polyamine levels. Plant Sci: 126 1-10
Lee TM, Lur HS, Lin TH, Chu C (1996) Physiological and biochemical changes in relation to methyl jasmonate-induced chilling tolerance of rice (Oryza sativa L.) seedlings. Plant Cell Environ 19: 65-74
Lee TM, Lur HS, Shieh YJ, Chu C (1994) Level of abscisic acid in anoxia-or ethylene-treated rice (Oryza sativa L.) seedlings. Plant Sci 95: 125-131
Leonardi A, Heimovaara-Dijkstra S, Wang M (1995) Differential involvement of abscisic acid in dehydration and osmotic stress in rice cell suspension. Physiol Plant 93: 31-37
Levitt J (1980) Chilling, freezing and high temperature stresses. In J Levitt, ed, Response of plant to environmental stresses, Ed2, Vol 1. Academic Press, New York, pp 497
Liang P, Averboukh L, Keyomarsi K, Sager R, Pardee AB (1992) Differential display and cloning of messenger RNAs from human breast cancer versus mammary epithelial cells. Cancer Research 52: 6966-6968
Liang P, Bauer D, Averboukh L, Warthoe P, Rohrwild M, Muller H, Strauss M, Pardee AB (1995) Analysis of altered gene expression by differential display. Methods in Enzymology 254: 304-323
Liang P, Pardee AB (1992) Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257: 967-971
Liu J, Hara C, Umeda M, Zhao Y, Okta TW, Uchimiya H (1995) Analysis of randomly isolated cDNAs from developing endosperm of rice (Oryza sativa L.): evaluation of expressed sequence tags and expression levels of mRNAs. Plant Mol Biol 29: 685-689
Liu K, Kasuga M, Sakumu Y, Abe H, Miura S, Yamaguchi-Shinozaki K, Sinozaki K (1998) Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell 10: 1391-1406
Lohmann J, Schickle H, Bosch TCG (1995) REN display, a rapid and efficient method for nonradioactive differential display and mRNA isolation. BioTechniques 18: 200-201
Lurie S, Klein JD (1991) Acquisition of low-temperature tolerance in tomatoes by exposure to high-temperature stress. J Am Soc Hortic Sci 116: 1007-1012
Lyons JM (1973) Chilling injury in plants. Ann Rev Plant Physiol 24: 445-466
Mackill D J, Lei X (1997) Genetic variation for traits related to temperature adaptation of rice cultivars. Crop Sci 37: 1340-1346.
Mangrich ME, Salveit ME (2000) Heat shocks reduced chilling sensitivity of cotton, kenaf, okra, and rice seedling radicles. J Am Soc Hortic Sci 125: 377-382
Mantyla E, Lang V, Palva ET (1995) Role of abscisic acid in drought-induced freezing tolerance, cold acclimation, and accumulation of LTI78 and RAB18 proteins in Arabidopsis thaliana. Plant Physiol 107: 141-148
McWillin JR, Kramer PJ, Musser RL (1982) Temperature-induced water stress in chilling-senstive plants. Aust J Plant Physiol 9: 343-352
Medina J, Bargues M, Terol J, Perez-Alonso M, Salinas J (1999) The Arabidopsis CBF gene family is composed of three genes encoding AP2 domain-containing proteins whose expression is regulated by low temperature but not by abscisic acid or dehydration. Plant Physiol 119: 463-469
Minami E, Kuchitsu K, He DY, Kouchi H, Midoh N, Ohtsuki Y, Shibuya N (1996) Two novel genes rapidly and transiently activated in suspension-cultured rice cells by treatment with N-acetylchitoheptaose, a biotic elicitor for phytoalexin production. Plant Cell Physiol 37: 563-567
Mohapatra SS, Poole RJ, Dhindsa RS (1987) Changes in protein patterns and translatable messenger RNA populations during cold acclimation of alfalfa. Plant Physiol 84: 1172-1176
Mohapatra SS, Wolfraim L, Poole RJ, Dhindsa RS (1989) Molecular cloning and relationship to freezing tolerance of cold acclimation-specific genes of alfalfa. Plant Physiol 89: 375-380
Moons A, Bauw G, Prinsen E, Van Montagu M, Van Der Straeten D (1995) Molecular and physiological responses to abscisic acid and salts in roots of salt-sensitive and salt-tolerant indica rice varieties. Plant Physiol 107: 177-186
Morgan JM (1991) A gene controlling difference in osmoregulation in wheat. Aust J Plant Physiol 18: 249-257
Mundy J, Chua NH (1988) Abscisic acid and water-stress induce the expression of a novel rice gene. EMBO J 7: 2279-2286
Nagamine T, Nakagahra M (1990) Genetic variation of chilling injury at seedling stages in rice, Oryza sativa L. Jpn J Breed 40: 449-455
Oh BJ, Balint DE, Giovannoni JJ (1995) A modified procedure for PCR-based differential display and demonstration of use in plants for isolation of genes related to fruit ripening. Plant Mol Bio Rep 13: 70-81
Orr W, Johnson AM, Keller WA Singh J (1990) Induction of freezing tolerance in microspore-derived embryos of winter Brassica napus. Plant Cell Rep 8: 579-581
Orr W, White TC, Iu B, Robert L, Singh J (1995) Characterization of a low-temperature-induced cDNA from winter Brassica napus encoding the 70 kDa subunit of tonoplast ATPase. Plant Mol Biol 28: 943-948
Pan A, Hayes PM, Chen F, Chen THH, Blake T, Wright S, Karsai I, Bedo Z (1994) Genetic analysis of the components of winterhardiness in barley (Hordeum vulgare L.). Theor Appl Genet 89: 900-910
Pan RC (1990) The role of abscisic acid in chilling resistance. In RP Pharis and SB Rood, eds, Plant Growth Substances, Spring-Verlag, Hong Kong, pp 391-399
Reaney MJT, Gusta LV, Robertson AJ (1989) The effect of abscisic acid, kinetin, and gibberellin on freezing tolerance in smooth bromegrass (Bromus inermis) cells suspensions. Can J Bot 67: 3640-3646
Rikin A, Richmond AE (1976) Amelioration of chilling injuries in cucumber seedlings by abscisic acid. Plant Physiol 38: 95-97
Robertson AJ, Gusta LV, Reaney MJT, Ishikawa M (1988) Identification of proteins correlated with increased freezing tolerance in bromegrass cell cultures. Plant Physiol 86: 344-347
Ryu SB, Costa A, Xin X, Li PH (1995) Induction of cold hardiness by salt stress involves synthesis of cold- and abscisic acid responsive proteins in potato (Solanum commersonii Dun). Plant Cell Physiol 36: 1245-1251
Sabehat A, Lurie S, Weiss D (1998) Expression of small heat-shock proteins at low temperatures. A possible role in protecting against chilling injuries. Plant Physiol 117: 651-658
Sambrook J, Fritsch EF, Maniatis T (1989) ²Molecular cloning². A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Sarhan F, Danyluk J (1998) Engineering cold-tolerant crops-throwing the master switch. Trends Plant Sci 3: 289-290
Sasahara T, Kambayashi M, Komiya K, Kim C (1982) Inheritance of cold tolerance at early growing and maturing stages in rice (Oryza sativa L.) Jpn J Breed 32: 311-316
Sasaki T (1997) Tolerance for environmental stress. In Matsuo T, Futsuhara F, Yamaguchi H, eds, Science of the Rice Plant, Vol 3. Genetics, Food and Agriculture Policy Research Center, Tokyo, pp 534-550
Sasaki T, Song J, Kong-Ban Y, Mastsui E, Fang F, Higo F, Nagasaki H, Hori M, Miya M, Murayama-Kayano E, Takiguchi T, Takasuga A, Niki T, Ishimaru K, Ikeda H, Yamamoto Y, Mukai Y, Ohta I, Miyadera N, Havukkula I, Minobe Y (1994) Toward cataloguing all rice genes: large-scale sequencing of randomly chosen rice cDNAs from a callus cDNA library. Plant J 6: 615-624
Sato K, Park KB (1982) On the low temperature damage in rice seedling. IV. Effect of low temperature on electrical conductivity and mineral element contents in water effusate of leaf blades, and their varietal differences. Jpn J Crop Sci 51: 215-220
Sembdner G, Parthier B (1993) The biochemistry and the physiological and molecular actions of jasmonates. Annu Rev Plant Physiol Plant Mol Biol 44: 569-589
Shinozaki K, Yamaguchi-Shinozaki (1997) Gene expression and signal transduction in water-stress response. Plant Physiol 115: 327-334
Shirzadegan M, Christie P, Seemann JR (1991) An efficient method for isolation of RNA from tissue cultured plant cells. Nucleic Acid Res 19: 6055
Smith PG, Dale JE (1988) The effects of root cooling and excision treatments on the growth of primary leaves of Phaselous vulgaris L. : rapid and reversible increases in abscisic acid content. New Phytol 110: 293-300
Song P, Yamamoto E, Allen RD (1995) Improved procedure for differential display of transcripts from cotton. Plant Mol Bio Rep 13: 174-181
St. John TP, Davis RW (1979) Isolation of galactose-inducible DNA sequences from saccharomyces cerevisiae by differential plaque filter hybridization. Cell 16: 443-452
Sthapit BR, Witcombe JR, Wilson JM (1995) Methods of selection for chilling tolerance in Nepalese rice by chlorophyll fluorescence analysis. Crop Sci 35: 90-94
Sutton F, Ding X, Kenefick DG (1992) Group 3 LEA gene HVA1 regulation by cold acclimation and deacclimation in two barley cultivars with varying freeze resestance. Plant Physiol 99: 338-340
Takahashi R, Joshee N, Kitagawa Y (1994) Induction of chilling resistance by water stress, and cDNA sequence analysis and expression of water stress-regulated genes in rice. Plant Mol Biol 26: 339-352
Thomashow MF (1990) Molecular genetics of cold acclimation in higher plants. Adv Genet 28: 99-131
Thomashow MF (1994) Arabidopsis thaliana as a model for studying mechanisms of plant cold tolerance. In E Meyerowitz, C Somerville, eds, Arabidopsis. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp 807-834
Trentmann SM, van der Kanap E, Kende H (1995) Alternative to 35S as a label for the differential display of eukaryotic messenger RNA. Science 267: 1186-1187
Uchimiya H, Kidou S, Shimazaki T, Aotsuka S, Takamatsu S, Nishi R, hashimoto H, Matsubayashi Y, Kidou N, Umeda M, Kato A (1992) Random sequencing of cDNA libraries reveals a variety of expressed genes in cultured cells of rice (Oryza sativa L.). Plant J 2: 1005-1009
Umeda M, Hara C, Matsubayashi Y, Li HH, Liu Q, Tadokoro F, Aotsuka S, Uchimiya H (1994) Expressed sequence tags from cultured cells of rice (Oryza sativa L.) under stressed conditions: analysis of transcripts of genes engaged in ATP-generating pathways. Plant Mol Biol 25: 469-478
Van der Knaap E, Kende H (1995) Identification of a gibberellin-induced gene in deepwater rice using differential display of mRNA. Plant Mol Biol 28: 589-592
Van Zee K, Chen FQ, Hayes PM, Close TJ, Chen THH (1995) Cold-specific induction of a dehydrin gene family member in barley. Plant Physiol 108: 1233-1239
Vernieri P, Pardossi A, Togoni F (1991) Influence of chilling and drought on water relations and abscisic acid accumulation in bean. Austral J Plant Physiol 18: 25-35
Walker-Simmons M (1987) ABA levels and sensitivity in developing wheat embryos of sprouting resistant and susceptible cultivars. Plant Physiol 84: 61-66
Wang CY (1991) Effect of abscisic acid on chilling injury of Zucchini squash. J Plant Growth Regul 10: 101-105
Wang CY, Buta JG (1994) Methyl jasmonate reduces chilling injury in Cucurbita pepo through its regulation of abscisic acid and polyamine levels. Environ Exp Bot 34: 427-432
Ward DA, Lawlor DW (1990) Abscisic acid may mediate the rapid thermal acclimatization of photosynthesis in wheat. J Exp Bot 41: 309-314
Weaver KR, Caetano-Anolles G, Gresshoff PM, Callahan LM (1994) Isolation and cloning of DNA amplification products from silver-stained polyacrylamide gels. BioTechniques 16: 226-227
Wilkinson JQ, Lanahan MB, Conner TW, Klee HJ (1995) Identification of mRNAs with enhanced expression in ripening strawberry fruit using polymearse chain reaction differential display. Plant Mol Biol 27: 1097-1108
Wolfraim LA, Dhindsa RS (1993) Cloning and sequencing of the cDNA for cas17, a cold acclimation-specific gene of alfalfa. Plant Physiol 103: 667-668
Wolfraim LA, Langis R, Tyson H, Dhindsa RS (1993) cDNA sequence, expression, and transcript stability of a cold acclimation specific gene, cas18, of alfalfa (Medicago falcata) cells. Plant Physiol 101: 1275-1282
Yamaguchi-Shinozaki K, Mino M, Mundy J, Chua NH (1990) Analysis of an ABA-responsive rice gene promoter in transgenic tobacco. Plant Mol Biol 15: 905-912
Yamamoto K, Sasaki T (1997) Large-scale EST sequencing in rice. Plant Mol Biol 35: 135-144
Yamane H, Tagagi H, Abe T, Yokata T, Takahashi N (1981) Identification of jasmonic acid in three species of higher plants and its biological actives. Plant Cell Physiol 22: 689-697
Yoshida H, Kato A (1994) Cold-induced accumulation of RNAs and cloning of cDNAs related to chilling injury in rice. Breeding Sci 44: 361-365
Yoshida H, Kato A (1994) Cold-induced accumulation of RNAs and cloning of cDNAs related to chilling injury in rice. Breeding Sci 44: 361-365
Zeevaart JAD, Creelman RA (1988) Metabolism and physiology of abscisic acid. Annu Rev Plant Physiol Plant Mol Biol 39: 439-473
Zhang J, Davies WJ (1989) Abscisic acid produced in dehydrating roots may enable the plant to measure the water status of the soil. Plant Cell Environ 12: 73-81
Zhang J, Davies WJ (1990) Does ABA in the xylem control the rate of leaf growth in soil-dried maize and sunflower plants. J Exp Bot 41: 1125-1132
Zhang J, Davies WJ (1991) Antitranspirant activity in xylem sap of maize plants. J Exp Bot: 42: 317-321
Zhu B, Chen THH, Li PH (1995a) Activation of two osmotin-like protein genes by abiotic stimuli and fungal pathogen in transgenic potato plants. Plant Physiol 108: 929-937
Zhu B, Chen THH, Li PH (1995b) Expression of three osmotin-like protein genes in response to osmotic stress and fungal infection in potato. Plant Mol Biol 28: 17-26
Zhu B, Chen THH, Li PH (1996) Analysis of late-blight disease resistance and freezing tolerance in transgenic potato plants expressing sense and antisense genes for an osmotin-like protein. Planta 198: 70-77
Zimmermann CR, Orr WC, Leclerc RF, Branard EC, Timberlake WE (1980) Molecular cloning and selection of genes regulated in aspergillus development. Cell 21: 709-715
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