臺灣博碩士論文加值系統

摘 要
許多疾病的起因與組織中各類型膠原蛋白含量有密切的關聯性，因此建立一套簡便、靈敏且精確之各類型膠原蛋白定量分析方法，不僅在科學研究上有其必需性，在臨床醫學上更具有重要性。本研究的目的，是利用毛細管電泳儀建立定量試樣中總膠原蛋白的方法，及使用電腦輔助各類型膠原蛋白序列及多胜的理論分析，然後進行膠原蛋白胜之毛細管電泳，並找出最佳緩衝溶液條件，以精確、靈敏地分析試樣中各種類型膠原蛋白的含量。
首先，利用實驗室研發的條件緩衝溶液進行膠原蛋白毛細管電泳，研究分析圖譜中膠原蛋白特定峰的共通性與專一性，在確定上述緩衝溶液條件的可行性之後，依此條件建立定量總膠原蛋白濃度的標準曲線，達到快速、簡便、正確定量試樣中總膠原蛋白含量的目標。
由於不同類型膠原蛋白之基酸組成有50﹪∼60﹪相同，且分子量也都在300,000左右，以致不同類型膠原蛋白彼此間之荷質比(charge/ mass)相當接近。為了解析不同種類的膠原蛋白，提高分析物間荷質比差異進而達到分離效果。本研究室先利用生物資訊網路及電腦輔助，預測分離不同類型膠原蛋白的有效方法。接著經過處理以得到膠原蛋白多胜，然後以毛細管電泳儀分析，並探討以毛細管電泳儀有效分離不同類型膠原蛋白的可行性。

目 錄
目錄………………………………………………….…. I
圖目錄………………………………………………....IV
表目錄…………………………………………………..Ｖ
中文摘要……………………………………………....VI
英文摘要…………………………………..……….….VII
第一章 緒論…………………………………….……. 1
第二章 實驗材料與方法………………………………28
第四章 各類膠原蛋白序列及多胜的理論分析.… 50
第五章 毛細管電泳分析不同類型的膠原蛋白………79
參考文獻……………………………………………….96

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