(3.236.222.124) 您好!臺灣時間:2021/05/10 15:05
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

: 
twitterline
研究生:余姿瑩
研究生(外文):Tz-Ying Yu
論文名稱:歸脾湯對去卵巢母鼠引起氧化應力的影響
論文名稱(外文):Effect of Gui-Pi-Tang on oxidative stress induced by ovariectomy in female rats
指導教授:林文川林文川引用關係
學位類別:碩士
校院名稱:中國醫藥學院
系所名稱:中國藥學研究所
學門:醫藥衛生學門
學類:藥學學類
論文種類:學術論文
論文出版年:2001
畢業學年度:89
語文別:中文
論文頁數:95
中文關鍵詞:歸脾湯去卵巢氧化應力
外文關鍵詞:Gui-Pi-Tangovariectomyoxidative stress
相關次數:
  • 被引用被引用:0
  • 點閱點閱:179
  • 評分評分:系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔
  • 下載下載:25
  • 收藏至我的研究室書目清單書目收藏:1
母鼠雙側卵巢切除後會使腦部脂質過氧化程度上升,投予17-estradiol可消除此上升作用,這些證實雌激素具有抗氧化作用。我們探討中藥方劑歸脾湯對去卵巢大鼠誘發腦部脂質過氧化的影響。去卵巢大鼠餵食含歸脾湯1 %、3%或5 % 的飼料12週。歸脾湯有意義減少去卵巢大鼠大腦皮質、紋狀體和海馬迴不加鐵誘發和加鐵誘發的脂質過氧化值,對加鐵與不加鐵誘發脂質過氧化差值也有抑制作用。以生體活性氧如超氧陰離子、氫氧自由基和歸脾湯作用來評估其抗氧化性質。歸脾湯對超氧陰離子清除能力不佳,但可以很快和氫氧自由基作用,其二次反應常數為1.10 *10 10 / M / sec。由這些結果推測歸脾湯對去卵巢大鼠腦部氧化傷害的保護作用與其清除自由基的能力有關。

The increase in the lipid peroxidation (LPO) level in the brain of female rats after bilateral ovariectomy, and this increase was abolished upon administration of 17-estradiol. These results evidenced that estrogen present antioxidant activity in brain of female rats. We examined the effects of Gui-Pi-Tang (GPT), a traditional Chinese medicine, on increased LPO level in the brain of ovariectomized (OVX) rats. Ovariectomized rats were provided with a diet containing 1 %, 3 % or 5 % GPT for 12 weeks. GPT significantly decreased 1) the contents of Fe-independent and Fe-dependent LPO concentrations in cerebral cortex, striatum and hippocampus in OVX rats; 2) the differences between Fe-stimulated level of LPO and the basal level in same examined tissues.The antioxidant properties of GPT were also evaluated by studying the ability of this drug to reactive with relevant biological oxidants such as superoxide anion radical and hydroxyl radical. GPT is not good scavenger of superoxide anion. However, it reacts rapidly with OH radical with a second-order rate constant 1.10 * 10 10 / M / sec. Our results suggested that the protection against oxidative damage by GPT in the OVX rat brain may be due to its scavenging activity against free radicals.

中文摘要……………………………………………………………………..1
表目錄………………………………………………………………………..2
圖目錄………………………………………………………………………..3
附錄(目錄)....……………………………………………………………..5
第一章 總論…………………………………………………………………6
第二章 大鼠去卵巢增加脂值過氧化及抗氧化系統的影響……………..12
第三章 歸脾湯對去卵巢大鼠氧化應力相關指標的影響………………..32
第四章 歸脾湯活體外抗氧化作用的研究………………………………..54
第五章 歸脾湯對去卵巢大鼠誘發骨質疏鬆的影響……………………..67
結論…………………………………………………………………………76
參考文獻……………………………………………………………………78
附錄…………………………………………………………………………88
英文摘要…………………………………………………………………....93
謝辭…………………………………………………………………………95

參考文獻
1. 金禮蒙等編:醫方類聚,卷59,,1978,中華世界資料供應出版社,
臺北。
2. 宋  陳自明撰,明  薛立齋註:校註婦人良方,卷24,瘡瘍門,
婦人結核方論第四,28-29,1977,旋風出版社印行,臺北。
3. 吳啟瑞:歸脾湯對中樞神經系統及避暗學習反應之影響,3-7,1992,
中國醫藥學院中國藥學研究所碩士論文,臺中。
4. 王平:中醫十大名方。歸脾湯,劉玉茂主編。中國中醫藥出版社,北
京, 1998。
5. 謝鳴:中醫方劑現代研究。歸脾湯,劉敬閣、姜勁邁主編。學苑出版
社,北京,上卷,p.614-622,1997。
6. 顏兆熊:停經婦女補充女性荷爾蒙之利弊。台灣醫界 42, 517-522,
1999。
7.謝明村、吳啟瑞、蔡輝彥、彭文煌、謝佳璋:歸脾湯對於大鼠被動迴避
學習反應之影響,中國醫藥學院雜誌 1994, 3, 15-24。
8.於慶海、吳春福、莊麗萍:歸脾湯對小鼠記憶行為和膽鹼酯脢活性的影響,中藥藥理與臨床 1990, 6, 2-3。
9.吳春福、于慶海、庄麗萍、徐問宇、劉保成、劉雯、郭月英:歸脾湯的抗氧化作用,中國中藥雜誌 1991, 16, 752-753。
Aebi, H.(1984) Catalase in vitro. Method Enzymol. 250,121-126.
Aust, S.D., Morehouse, L.A. and Thomas, C.E. (1985) Oxygen
toxicity, oxygenradicals, transition metals and disease.
Free Radi. Biol. Med. 1, 3-25.
Bain SD.(1993) Bailey MC. Celino DL. Lantry MM. Edwards MW.
High-dose estrogen inhibits bone resorption and stimulates
bone formation in the ovariectomized mouse. J Bone Miner Res.
8(4):435-42
Barness, L.A.,. Mellman, W.J. Tedesco, T.,. Young, D.G,and
Nocho.R.A.(1963) A quantiative method for determining uniary
phenols. Clin. Chem. 9, 600-607
Behl, C.,WIDMANN, M ., TRAPP, T. & HOLSBOER , F. (1995). 17-β
Estradiol protect neurons from oxidative stress-induced cell
death in vitro .Biochem. Biophys. Res. Commun.., 216, 473-482.
Behl C. (1999)Vitamin E and other antioxidants in
europrotection.International Journal for Vitamin & Nutrition
Research. 69(3):213-9.
Benzi,G and Moretti, A. (1995) Age- and peroxidative stress-
related modifications of the cerebral enzymatic activities
linked to mitochondria and the glutathione system. Free
Radic. Biol. Med. 19, 77-101.
Birge, S.J. (1998) Hormones and the aging brain. Geriatrics 53,
S28-S30.
Boveris, A., Oshino, N. and Chance, B. (1972) The cellular
production of H2O2. Biochem. J. 128, 617-630.
Braughler, J.M., Pregenzer, J.F., Chase, R.L., Duncan, L.A.,
Jacobsen, E.J. and McCall, J.M. (1987) Novel 21-amino steroid
as potent inhibitors of iron-dependent lipid peroxidation. J.
Biol. Chem. 262, 10438-10440.
Bromont, C., Marie, C. and Bralet, J. (1989) Increased lipid
peroxidationin vulnerable brain regions after transient
forebrain ischemia in rats. Stroke 20, 918-924.
Burnett, CC, Reddi AH|(1983) Influence of estrogen and
progesterone on matrix-induced endochondral bone
formation.Calcif Tissue Int.35.609-614.
Catherine, A. Rice-Evans and ANTHONY T.Diplock.(1993)Current
Status of Antioxidant Therapy. Free Radi. Biol. Med .Vol.
15,pp.77-96
Delanty N. Dichter MA.(2000)Antioxidant therapy in neurologic
disease. [Review] [71 refs]Arch Neurol. 57(9):1265-70
Dreosti,I.E.(1991) Free radical pathology and the genome.In
Trace Elements. Micronutrients and Free Radicals .
(I.E.Dreosti,ed.)p.149-170,Humana Press, Clifton,NJ USA
Evans, P.H. (1993) Free radicals in brain metabolism and
pathology. Bri. Med. Bull. 49, 577-587.
Falkeborn M. Persson I. Terent A. Adami HO. Lithell H.
Bergstrom R. (1993) Hormonereplacement therapy and the risk
of stroke. Follow-up of a population-based cohort in Sweden.
Arch Inter Med.153 (10):1201-9
Fillit H. Weinreb H. Cholst I. Luine V. McEwen B. Amador R.
Zabriskie J (1986)Observations in a preliminary open trial of
estradiol therapy for senile dementia-Alzheimer's
type.Psychoneuroendocrinology. 11(3):337-45
Garrido, A., Garate, M., and Valenzulea, A. (1993) Changes in
the antioxidant capacity of blood plasma are produced after
Res comm Chem Pathol Pharmacol. 367-370.
Gibbs, R.B. and Aggarwal, P. (1998) Estrogen and basal
forebrain cholinergic neurons: implications for brain aging
and Alzheimer’s diseae-related cognitive decline. Horm and
Behav 34, 98-111.
Glowinski J. Iversen LL. Axelrod J.(1966) Storage and synthesis
of norepine-hrine in the reserpine-treated rat brain.J
Pharmacol Exp Ther.151(3):385-99
Gomez-Zubeldia MA. Hernandez R. Viguera J. Arbues JJ. Aparicio
A. Millan JC. (2000)Effect ofbilateral ovariectomy and
ovarian steroid hormones on the antioxidant systems and
plasmamalondialdehyde levels in Wistar rats. Endocr Res. 26
(1):97-107
Goodman .Y,. Bruce, A. J., Cheng, B.& Mattson , M. P.(1996).
Estrogens attenuate and corticosterone exacerbates
excitotoxicity , oxidative injury , and amyloid β-peptide
toxicity in hippocampal neurons .J. Neurochem., 66,1836-1844.
Green PS. Gordon K. Simpkins JW. (1997) Phenolic A ring
requirement for the neuroprotective effects of steroids.
Journal of Steroid Biochemistry & Molecular Biology. 63(4-
6):229-35.
Gutteridge, J.M.C. (1984) Reactivity of hydroxyl and hydroxyl-
like radicals discriminated by release of thiobarbituric acid-
reactive material from deoxy sugars, nucleosides and
benzoate. Biochem. J. 224, 761-767.
Henderson VW.(1997) The epidemiology of estrogen replacement
therapy and Alzheimer's disease.[Review] [142 refs]
Neurology. 48(5 Suppl 7):S27-35
Henderson, V.W., Watt, L. and Buckwalter, J.G. (1996) Cognitive
skills associated with esterogen replacement in women with
Alzhelmer’s disease. Psychoneuroendcrinology 21, 421-430.
Henderson VW. Paganini-Hill A. Emanuel CK. Dunn ME. Buckwalter
JG. (1994)Estrogen replacement therapy in older women.
Comparisons between Alzheimer's disease cases and
nondemented control subjects. Arch Neurol. 51(9):896-900
Halliwell, B., Gutteridge, J.M.C. and Aruoma, I. (1987) The
deoxyribose method: a simple “test-tube” assay for
determination of rate constants for reactions of hydroxyl
radicals. Anal. Biochem. 165, 215-219.
Hanfeman, DG, Sunde, RA, and Hoekstra, WG.(1974) Effect of
dietary selenium on erythrocyte and liver glutathione
peroxidase in the rats. J Nutr .104, 580-587.
Jorm AF. Korten AE. Henderson AS.(1987) The prevalence of
dementia: a quantitative integration of the literature [see
comments]. [Review] [62 refs] Acta Psychiatr Scand. 76(5):
465-79
Kalu, D.N. , Hardin, RR, Cockerhan, R.(1984) Evaluation of the
pathogenesis of skeletal change in ovariectomized rats.
Endocrinology.115,507-512.
Kalu, D.N. , Hardin, RR,(1984) Evaluation of the role of
calcitonin deficiency in ovariectomized-induce osteopenia.
Life Sci.34 ,2394-2397.
Kalu DN.(1991) The ovariectomized rat model of postmenopausal
bone loss. [Review] [98 refs]Bone Miner. 15(3):175-91, 1991
Kalu, D.N., Liu, C.C., Salerno, E., Hollis, B., Echon, R. and
Ray, M. (1991).Skeletal response of ovariectomlized rats to
low and high does of 17-estradiol.Bone Miner.14,175-187.
Kawas, C., Resnick, S., Morrison, A., Brookmeyer, R., Corrada,
M., Zonderma, A., Bacal, C., Lingle, D.L. and Metter, E.
(1997) A prospective study of estrogen replacement therapy
and the risk of developing Alzheimer’s disease: The
Baltimore Longitudinal Study of Aging. Neurology 48, 1517-
1521.
Kasapovic, J., Pajovic, S.B., Kanazir, D.T. and Martinovic,
J/V. (1997) Effect of estradiol benzoate and progesterone on
superoxide dismutase activity in the rat liver. J.
Endocrinol. Invest. 20, 203-206.
Kume-Kick, J., Ferris, D.C., Russo-Menna, I. And Rice, M.E.
(1996) Enhanced oxidative stress in female rat brain after
gonadectomy. Brain Res. 738, 8-14.
Lindsay R.(1987) Estrogen therapy in the prevention and
management of osteoporosis. Am J Obstet Gynecol. 156(5):1347-
51
Liu,C.C.,Kalu, D.N., (1990) Preexisting bone loss associated
with ovariectony is reversed by parathyroid hormone.J Bone
Min Res. 5:239.
Lovell, M.A., Ehmann, W.D., Butler, S.M. and Markesbery, W.R.
(1995) Elevated thiobarbituric acid-reactive substance and
antioxidant enzyme activity in the brain in Alzheimer’s
disease. Neurology 45, 1594-1601.
Lowry, O.H. , Rosebrough, N.J. , Farr, A.L. , and Randall, R.J.
(1951) Protein measurment with the folinophenol reagent .
J.Biol. Chem.193,265-275.
Markesbery, W.R. and Ehmann, W.D. (1993) Brain trace elements
in Alzheimer disease. In: Tery, R.D., Kaizman, R. and Bick,
K.L. eds. Alzheimer disease. New York: Raven Press; p.353-367.
Markesbery WR.(1997) Oxidative Stress Hypothesis in Alzheimer's
disease. Free Radic. Biol. Med. 23(1):134-47
Markesbery WR. Carney JM.(1999) Oxidative alterations in
Alzheimer's disease. [Review] [136refs] Brain Pathology. 9
(1):133-46
Marklund, S.and Marklund, G.(1975).Involvement of superoxide
anion radical in the autoxication of dyrogallol and
convenient assay for superoxide dismutase. Eur J. BioChem.
47, 469-474.
Mcintosh, L.J., Trush, M.A. and Tronocoso, J.C. (1997)
Increased susceptibility of Alzheimer’s disease temporal
cortex to oxygen free radical-mediated processes. Free Radic.
Biol. Med. 23, 183-190.
Mohamed MK. Abdel-Rahman AA.(2000) Effect of long-term
ovariectomy and estrogen replacement on the expression of
estrogen receptor gene in female rats. European Journal of
Endocrinology. 142(3):307-14
Moosmann B. Behl C.(1999) The antioxidant neuroprotective
effects of estrogens and phenolic compounds are independent
from their estrogenic properties. Proc Natl Acad Sci U S A.
96(16):8867-72
Norman Delanty ,MD; Marc A. Dichter ,MD ,PhD(2000)Antioxidant
Therapy in Neurologic Disease. Arch. Neurol. Vol 57 :1265-
1270
Ohkawa, H., Ohishi, N. and Yagi, K.(1979) Assay for lipid
peroxides in animal tissue by thiobarbituric acid reaction.
Anal.Biochem.95,351-358.
Ohkura T. Isse K. Akazawa K. Hamamoto M. Yaoi Y. Hagino N.
(1994)Evaluation of estrogentreatment in female patients
with dementia of the Alzheimer type. Endocrine Journal. 41
(4):361-71
Patlas N. Zadik Y. Yaffe P. Schwartz Z. Ornoy A. (2000)
Oophorectomy-induced osteopenia in rats in relation to age
and time post oophorectomy. Cells Tissues Organs.166(3):
267-74
Peris, P., Alvarez, L., Monegal, A., Guanabens, N., Duran, M.,
Pons, F., Martinez de Osaba, M.J., Echevarria, M., Ballesta,
A.M. and Munoz-Gomez, J. (1999) Biochemical markers of bone
turnover after surgical menopause and hormone replacement
therapy. Bone25, 249-353.
Persky, A.M., Green, P.S., Stubley, L., Howell, C.O.,
Zaulyanov, L., Brazeau, G.A. and Simpkins, J.W. (2000)
Protective effect of estrogen against oxidative damage to
heart and skeletal muscle in vivo and in vitro. Proc. Soc.
Exp. Biol. Med. 223, 59-66.
Ramassamy, C., Averill, D., Beggert, V. Bastiantetto,
S.,Theroux, L.,Lussier-Cacan, S.,Cohn, J.S.,Christen,
Y.Davignen, J. Quirion, R. and Poirier, J.(1999)Oxidative
damage and protection by Antioxidants in the frontal cortex
of Alzheimer’s disease is related to the Apolipoprotein E,
Genotype. Free Radic Biol Med. 27 , 544-553.
Reiter, R.J. (1995) Oxidative processes and antioxidative
defense mechanisms in the aging brain. FASEB J. 9, 526-533.
Rice-Evans, C.A. and Diplock, A.T. (1993) Current status of
antioxidant therapy. Free Radic. Biol. Med. 15, 77-91.
Robert, B. and Aggarwal, P. (1998) Estrogen and basal forebrain
cholinergic neurons: implications for brain aging and
Alzheimer’s disease-related cognitive decline. Horm Behav
34, 98-111。
Sahoo, A. and Chainy, G.B.N. (1997) Alterations in the
activities of cerebral antioxidnt enzymes of rat are related
to aging. Int. J. Neurosci. 15, 939-948.
Sharma, H.M., Hanna, A.N., Kauffman, E.M. and Newman, H.A.I.
(1995) Effect of herbal mixyure student rasayana on
lipoxygenase activity and lipid peroxidation. Free Radic.
Biol. Med. 18, 687-697.
Sohal, R.S. and Donato, H.J. (1979)Effect of experimental
prolongation of life span on lipofuscin content and lysosmal
enzyme activity in the brain of the housefly,
Musca domestica. J. Gerontol. 34, 489-496.
Sreelathakumari KT. Menon VP. Leelamma S.(1993) Lipid peroxide
metabolism in oophorectomised rats. Indian J Med Res. 98:305-8
Sugioka K. Shimosegawa Y. Nakano M.(1987) Estrogens as natural
antioxidants of membranephospholipid peroxidation. FEBS
Letters. 210(1):37-9
Tang MX. Jacobs D. Stern Y. Marder K. Schofield P. Gurland B.
Andrews H. Mayeux R.(1996)Effect of oestrogen during
menopause on risk and age at onset of Alzheimer's disease
[see comments]. Lancet. 348(9025):429-32
Tian, L. Cai, Q and Wei, H. (1998) Alterations of antioxidant
enzymes and oxidative damage to macromolecules in different
organs of rats during aging. Free Radic. Biol. Med. 24, 1477-
1484.
Toran-Alleerand, C.D., Singh, M. and Setalo, G.J. (1999) Novel
mechanisms of estrogen action in the brain: new players in an
old story. Front Neuroendocrinol. 20, 97-121.
Tremollieres F. Pouilles JM. Ribot C.(1992). Postmenopausal
bone loss. Role of estrogens.[Review].Presse Med. 21(19): 903-6
Turner RT, Vanderstcenhoven, JJ, Bell, NH.(1987) The effect of
ovariectomy and 17-estadiol on cortical bone
histomophometry in growing rats.J. Bone Min Res.2.115-122.
Uchii M. Takashima M. Sugiyama T. Kosaka N.(1998) [Effect of KW-
8232 on bone turnover in ovariectomized rats]. [Japanese]
Nippon Yakurigaku Zasshi .112(5):315-21
Wang, Q., Santizo, R., Baughman, V.L., Pelligrino, D.A., and
Iadecola, C. (1999) Estrogen provides neuroprotection in
transient forebrain ischemia through perfusion-independent
mechanisms in rats. Stroke 30, 630-637.
Wiseman, H. (1995) Dietary influence on membrane function:
importance in protection against oxidative damage and
disease. J. Nutr. Biochem. 7, 2-15.
Wronski,T.J, Cintron M, Doherty AL,Dann LM. (1988) Estrogen
treatment prevents osteopenia and depress bone turnover in
ovariectomized rats.Endovrinology.123,681-686.
Yagi, K. (1997) Female hormones act as natural antioxidants a
survey of our research. .Acta Biochimica Polnica 44, 701-710.
Yanker, B.A., DUFFY, L.K. & KIRSCHNER, D.A.(1990).Neurotrophic
and neurotoxic effrcts of amyloid β protein :reversal by
tachykinin neuropeptides. Science , 250 ,279-282.
Yamada, K., Tanaka, T., Zou, L.B., Senzaki, K., Yano, K.,
Osada, T., Ana, O., Ren, X., Kameyama, T. and Nabeshima, T.
(1999) Long-term deprivation of oestrogens by ovariectomy
potentiates-amyloid-induced working memory deficits in rats.
Bri.J. Pharmacol. 128, 419-427.
Yin A. (1996) Biochemical basis of lipofuscin, ceroid, and age
pigment-like fluorophores. Free Radic. Biol. Med. 21, 871-888.

QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
無相關論文
 
系統版面圖檔 系統版面圖檔