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研究生:高嘉君
研究生(外文):Chia-Chun Kao
論文名稱:自體免疫性甲狀腺疾病患者血清中anti-Ro/SS-A與anti-La/SS-B抗體內同種型分佈之研究
論文名稱(外文):Isotype distribution of anti-Ro/SS-A and anti-La/SS-B antibodies in plasma of patients with autoimmune thyroid diseases
指導教授:蔡嘉哲蔡嘉哲引用關係
指導教授(外文):Gregory J. Tsai
學位類別:碩士
校院名稱:中山醫學院
系所名稱:醫學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:2001
畢業學年度:89
語文別:中文
論文頁數:31
中文關鍵詞:自體免疫性甲狀腺疾病同種型
外文關鍵詞:autoimmune thyroid diseaseanti-Ro/SS-Aanti-La/SS-BIsotype
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本研究收集57個Grave’s disease,25個Hashimoto’s thyroiditis且無其他自體免疫相關病症患者,和24個正常人共106支血清進行自體免疫性甲狀腺疾病患者血清中自體抗體分佈之研究發現,有高頻率抗Ro及抗La抗體存在的現象,並且以免疫球蛋白G(immunoglobulin-G)的同種型分佈最常見,免疫球蛋白A居次,免疫球蛋白M最少見,提示了日後在自體免疫性甲狀腺疾病檢查中,抗Ro及抗La抗體檢測的重要性、甲狀腺體分泌及抗原分佈的相關性和探討甲狀腺炎與Sjogren’s syndrome疾病間之關聯性與發展的方向。

Objective:
To study the isotype distribution of anti-Ro (SS-A) and anti-La (SS-B) antibodies in autoimmune thyroid diseases and the possible pathogenesis.
Materials and Methods:
A total of 106 serum samples from 57 patients with Grave’s disease, 25 patients with Hashimoto’s thyroiditis and 24 healthy controls were tested for the distribution of isotype of autoantibodies against the recombinant Ro 52kD, Ro 60kD, and La 48 kD protein by the enzyme-linked immunosorbent assay (ELISA). Immunoglobulin (Ig)G, IgM, and IgA antibodies to recombinant protein were detected by ELISA, too. For ELISA, a positive result was taken to be greater than 2 standard deviations above the mean of the normal group.
Results:
High prevalence of positive result of anti-Ro and anti-La antibodies in plasma of patients with autoimmune thyroid diseases. The distribution of isotype of
anti-La 48 Ab: IgG>IgA>IgM,
anti-Ro 52 Ab: IgG=IgA>IgM,
anti-Ro 60 Ab: IgG>IgA>IgM
Conclusion:
The prevalence of anti-Ro and anti-La antibodies and difference in distribution of isotype encourage further workup of autoimmune thyroid diseases with other autoimmune diseases.

誌 謝 I
中文摘要 II
ABSTRACT III
目 錄 IV
第一章 研究背景 1
第二章 材料與方法 5
第三章 結 果 8
第四章 討 論 10
參考文獻 18

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