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研究生:柯國楨
研究生(外文):Kerr Gour Jen
論文名稱:精漿及精蟲中乳酸脫氫酉每與肌酸激酉每活性及其同功酉每的研究評估
論文名稱(外文):THE STUDY OF LD AND CK ACTIVITIES AND THEIR ISOENZYMES IN SEMINAL PLASMA AND SPERM
指導教授:蔡麗玉蔡麗玉引用關係
指導教授(外文):LI-YU TSAI
學位類別:碩士
校院名稱:高雄醫學大學
系所名稱:醫學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:2000
畢業學年度:89
語文別:中文
論文頁數:104
中文關鍵詞:精液乳酸脫氫酉每肌酸激酉每天門冬胺酸轉胺酉每乳酸脫氫同功酉每肌酸激同功酉每乳酸脫氫酉每-C4乳膠電泳
外文關鍵詞:SEMENLACTATE DEHYDROGENASE (LD)CREATINE KINASE (CK)ASPARTATE TRANSAMINASE (AST)LD ISOENZYMECK ISOENZYMELD - C4AGAROSE ELECTROPHORESIS
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據統計,每年約有15 %的新婚夫婦有不孕的困擾,其中約有30-50%是因為男性原發性因素或因精液品質不良所引起。同時,國內外的文獻也指出傳統的精液分析無法滿足對精液品質評估的需求。因此我們進行精漿及精蟲中天門冬胺酸轉胺酉每(Aspartate transaminase :以下簡稱AST),乳酸脫氫酉每 (Lactate dehydrogenase;以下簡稱LD)與肌酸激酉每(Creatine Kinase;以下簡稱CK)等之活性測定及其同功酉每的分析來探討其作為精液品質評估之生化標幟的可行性。
本實驗隨機收集108位高雄市阮綜合醫院生殖醫學中心門診病人(其中正常精蟲數者81位,精蟲稀少症者22位,無精症者5位)之精液檢體,待完全液化後離心分離精漿並以Triton-x 100 萃取已清洗乾淨之精蟲中的酵素。本實驗採用OLYMPUS AU 600自動生化分析儀測定各酵素活性及使用瓊脂電泳法進行LD及CK同功酉每分析。
本研究結果顯示:1.無精症者精漿中完全無LD-C4存在,而所有病人之精漿檢體及嚴重精蟲稀少症者之精蟲檢體中之CK同功酉每全部是CK-1。 2.正常精蟲數者與精蟲稀少症者間有顯著差異之主要檢查項目的結果如下表所示。 3.比較正常精蟲數者與精蟲稀少症者之平均值比值時,發現這些分析項目中以精蟲CK活性之比值最高(1:20.2),表示在區分正常精蟲數者與精蟲稀少症者時,以精蟲CK活性的能力最好。 4.我們以相關分析統計證實這些分析項目中以精漿LD-C4百分比與精蟲數間的相關性最好( r=0.577 , P=0.000)。
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項 目 (單位) 正常精蟲數者   精蟲稀少症者
----------------------------------------------------------------
精漿
LD-C4 (%) 16.8±0.6 5.29±1.1a
( 80 )( 22 )
AST (IU/L) 261.4±8.7 214.8±20.6b
( 81 )( 22 )
CK (IU/L) 660.6±92.0 521.0±109.3
( 75 )( 21 )
CK-1 (%) 100 0
( 75 )( 21 )
精蟲
AST (mIU/106 sperms) 4.50±0.3 15.7±5.2a
( 78 )( 21 )
LD (mIU/106 sperms) 18.54±1.3 73.6±27.2a
( 77 )( 20 )
CK (mIU/106 sperms) 4.39±0.8 88.6±63.5c
( 72 )( 18 )
LD-C4 (%) 59.60±1.3 46.2 ±4.7a
( 79 )( 21 )
CK-3 (%) 14.9 ±1.4 4.6 ±1.9c
( 72 ) ( 18 )
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1.※ 結果以平均值 ±標準誤表示 ; 括弧內數字表示檢體數目。
2. 精蟲稀少症者vs.正常精蟲數者a P = 0.000 , bP < 0.05, cP < 0.01
由以上數據我們推測:精漿的LD-C4百分比(或活性); 精蟲的LD-C4百分比、CK-3百分比、AST活性、LD活性及CK活性都可做為反映精蟲成熟度的生化指標。其中以精蟲CK活性對精蟲數是否正常的區分能力最好,而在評估精蟲成熟度方面,以精漿LD-C4百分比是最適合的生化指標。

According to the statistics showed that there are about fifteen percents newly married couples have the worry of infertility in every year. About 30 to 50 percents of these resulted from the male primary factors or their poor semen quality. Moreover, literatures have been demonstrated that the conventional semen analysis couldn’t satisfy the need of the evaluation of semen quality.
The aim of this study is to evaluate the usability of the analysis of asparate oxaloacetate transaminase activity (AST), lactate dehydrogenase activity (LD), creatine kinase activity (CK) and LD as well as CK isoenzymes in seminal plasma and sperms for biochemical markers of the semen quality.
In this study, we randomly collected 108 outpatient’s semen samples from The Center of Reproductive Medicine in the Yuan’s General Hospital in Kaohsiung (81 normalspermic men, 22 oligospermic men, 5 azoospermic men ). After complete liquefaction, we separated the seminal plasma fraction by centrifugation and extracted enzymes from washed sperms by 0.2% triton-x 100. We determined enzymes activity by OLYMPUS AU600 biochemical autoanalyzer and measured LD and CK isoenzymes by agarose electrophoresis.
Results indicated that no LD-C4 present in the azoospermic seminal plasma samples and only CK-1 presents in all seminal plasma samples and severity oligospermic sperm samples. Data also showed that normalspermic men were significantly higher than oligospermic men in seminal plasma LD-C4 percent (16.8±0.6% vs.5.3±1.1% , P=0.000 , N=80 vs. 22), AST activity (261.4±8.7 IU/l vs.214.8±20.6 IU/L , P<0.05 , N=81 vs. 22), sperm LD-C4 percent (457.2±37.0% vs. 142.8±37.6% , P=0.000 , N=80 vs. 22) and CK-3 percent (14.9±1.4% vs.4.6±1.9% , P<0.01 , N=72 vs. 18). Inversely, oligospermic men were significantly higher than normalspermic men in sperm LD activity (73.63±27.17 mIU/106 sperms vs. 18.54±1.3 mIU/106 sperms , P=0.000 , N=20 vs. 77), CK activity (88.61±63.49 mIU/106 sperms vs. 4.39 ±0.8 mIU/106 sperms , P<0.01 , N=18 vs. 72 ) and AST activity (15.7±5.2 mIU/106 sperms vs. 4.5±0.3 mIU/106 sperms , P=0.000 , N=21 vs. 78). When compared to the mean value ratios of these items between normalspermic men and oligospermic men, the sperm CK activity was found that has highest ratio (1:20.2). This indicated that the sperm CK activity is the best in distinguish normalspermic men and oligospermic men. Additionally, the correlation analysis of these items demonstrated the best positive relationship between the seminal plasma LD-C4 percent and the sperm concentration (r=0.577, P=0.000).
These data suggested that the seminal plasma LD-C4 percent (or activity) and sperm LD-C4 percent, CK-3 percent, AST 、LD and CK activities were all good for biochemical markers to reflect sperm maturity. Especially the sperm CK activity has best power to distinguish either normal or not in sperm concentration. In terms of evaluating sperm maturity, the seminal plasma LD-C4 percent is the most suitable biochemical marker.

中文摘要---------------------------------------------------1
英文摘要---------------------------------------------------3
第一章 前言
第一節 研究背景------------------------------------------5
第二節 研究動機------------------------------------------6
第三節 研究目的------------------------------------------8
第二章 文獻回顧
第一節 簡介正常精蟲形成與荷爾蒙調控作用------------------9
第二節 男性不孕症的原因探討-----------------------------11
第三節 傳統精液分析的應用現況---------------------------13
第四節精液中乳酸脫氫酉每、肌酸激酉每及其同功酉每與
天門冬胺酸轉胺酉每活性的研究現況-----------------15
第三章 材料及方法
第一節受檢對象-----------------------------------------23
第二節精液檢體的收集及處理-----------------------------24
第三節傳統精液檢查項目的方析方法-----------------------26
第四節精漿及精蟲萃取液中乳酸脫氫酉每及肌酸激酉每活性及其
同功酉每與天門冬胺酸轉胺酉每活性的分析方法-------29
第五節統計分析方法-------------------------------------41
第四章 研究結果
第一節精液分析的結果-----------------------------------42
第二節精液中乳酸脫氫酉每活性及其同功酉每的分析結果-----44
第三節精液中肌酸激酉每活性及其同功酉每的分析結果-------47
第四節精液中天門冬胺酸轉胺酉每活性的分析結果-----------49
第五節精液中各種生化標幟間的相關酉每性及其診斷效力
的評估-------------------------------------------50
第六節精液中罕見乳酸脫氫酉每同功的分析結果-------------51
第五章 討論
第一節實驗方法的討論-----------------------------------53
第二節實驗結果的討論-----------------------------------54
第三節綜合討論-----------------------------------------58
第四節結論---------------------------------------------60
參考文獻 -------------------------------------------------61
表目錄
表一、以( A )反復冷凍解凍的方法及以( B ) 0.2% Triton-X的
方法來萃取精蟲酵素的結果比較------------------------73
表二、以0.2% Triton-X 100處理的精蟲檢體在 4℃下保存五天
之精蟲LD、CK、AST活性及Protein的變化情形------------74
表三、正常精蟲數者、精蟲稀少症者與無精症者的精液分析------75
表四、正常精蟲形態者與精蟲畸型症者的精液分析--------------76
表五、正常精蟲運動性者與精蟲無力症者的精液分析------------77
表六、正常精蟲數者及精蟲稀少症者在精蟲形態分類及運動性
分類中的分佈情形------------------------------------78
表七、正常精蟲數者、精蟲稀少症者與無精症者精液中LD同功
酉每百分比的分析------------------------------------79
表八、正常精蟲數者、精蟲稀少症者與無精症者精液中 LD 及其
同功酉每活性分析------------------------------------80
表九、精液中LD及其同功酉每活性與精蟲數、正常形態精蟲百分比
及前進運動性精蟲百分比間的相關性--------------------81
表十、正常精蟲數者、精蟲稀少症者與無精症者精液中CK同功酉每
百分比分析------------------------------------------82
表十一、正常精蟲數者、精蟲稀少症者與無精症者精液中CK及其
同功酉每的活性分析----------------------------------83
表十二、精液中CK及其同功酉每活性與精蟲數、正常形態精蟲百分
比及前進運動性精蟲百分比間的相關性------------------84
表十三、正常精蟲數者、精蟲稀少症者與無精症者精液中AST活
性分析----------------------------------------------85
表十四、精液中 AST 活性與精蟲數、正常形態精蟲百分比及前
進運動性精蟲百分比間的相關性------------------------86
表十五、精蟲數與精蟲中 LD、CK、AST、LD-C4 (%)及CK-3(%)
間的相關性------------------------------------------87
表十六、正常精蟲數者與精蟲稀少症者之LD-C4與CK-3百分比
及LD-C4與CK-3活性間的相關性-------------------------88
表十七、正常精蟲數者與精蟲稀少症者精液中LD、CK及AST活
性平均值的比值--------------------------------------89
表十八、各種生化指標在區分正常精蟲數者與精蟲稀少症者時之
敏感度及特異性分析----------------------------------90
圖目錄
圖一、精細胞減數分裂的DNA變化及形態變化-------------------91
圖二、LD 同功酉每之電泳條件調整前及調整後之電泳圖---------92
圖三、正常精蟲數者、精蟲稀少症者及無精症者精液中LD同功酉每
電泳圖----------------------------------------------93
圖四、正常精蟲數者、精蟲稀少症者及無精症者精漿LD同功酉每百
分比的分析------------------------------------------94
圖五、正常精蟲數者及精蟲稀少症者精蟲LD同功酉每百分比的
分析-----------------------------------------------95
圖六、正常精蟲數者、精蟲稀少症者及無精症者精液中CK
同功酉每電泳圖--------------------------------------96
圖七、CK 同功酉每電泳圖-----------------------------------97
圖八、精漿CK同功酉每百分比的分析--------------------------98
圖九、精漿LD、CK及AST活性分析-----------------------------99
圖十、精蟲LD、CK及ASR活性分析----------------------------100
圖十一、精漿LD-C4(%)、LD-C4 活性、AST活性、精蟲CK-3 (%)及
精蟲LD-C4(%)等生化指標之ROC Curve分析--------------101
圖十二、精蟲LD、CK、AST、LD-C4、CK-3活性等生化指標之ROC
Curve分析------------------------------------------102
圖十三、睪丸及精蟲中罕見LD同功酉每電泳圖-----------------103
圖十四、精液中罕見LD同功酉每電泳圖-----------------------104

1.Rowe, P.J. , Comhaire, F.H. , Hargreave, T.B. and Mellows, H.J. 1993. WHO Manual for the standardized Investigation and Diagnosis of the Infertile Couples. Cambridge University press, Cambridge.
2.Gilbert BR, Cooper GW, Goldsbein M. 1992. Semen analysis in the evaluation of male factor subfertility. AUA Update Series 11(30):250.
3.Carlsen E. Giwercman A, Keiding N, skahkebaek NE. 1992. Evidence for decreasing quality of semen during the past 50 years. BMJ 305: 609-613.
4.Auger J, Kunstman JM, Czyglik F, Jouannet P. 1995. Decline in semen quality among fertile men in paris during the past 20 years. N Engl J Med 332:281-285.
5.Irvine S, Cawood E, Richardson D, MacDonald E, Aitken J. 1996. Evidence of deteriorating semen quality in the United Kingdom: birth cohort study in 577 men in scotland over 11 years. BMJ 312:467-71.
6.Macleod J, Gold RA. 1951. The male factor in fertility and infertility.Ⅱ. Spermatozoa counts in 1000 cases of know fertility and 1000 cases of infertile marriage J Urol 66:436.
7.Smith KD et al. 1977. Relationship between indices of semen analysis and pregnancy rate in infertile couples. Fertil steril 28:1314.
8.Irvine, D.S. and Aithen, R.J. 1994. Seminal fluid analysis and sperm function testing. Endocr. Metab. Clin North Am 23:725-748.
9.Aitken, R.J., Best, F.S.M., Richardson, D.W. et al. 1982a. An analysis of sperm function in cases of unexplained infertility: Conventional criteria, movement characteristics, and fertilizing capacity. Fertil Steril 38: 212-221.
10.Aitken, R.J., Best, F.S.M., Richardson, D.W. et al. 1982b. An analysis of semen quality and sperm function in cases of oligozoospermia. Fertil steril 38:705-711.
11.Schwart, D Laplanche, A. Jouannet, P. and David, G. 1979. Within-subject variability of human semen in regard to sperm count, volume, total number of spermatozoa and length of abstinence. J Reprod Fertil 57: 391-395.
12.Mallidis, C., Howard, E.J. and Bakev, H.W. 1991. Variation of semen quality in normal men. Int J Androl 14:99-107.
13.Market CL, Shaklee JB, and Whitt GS. 1975. Evolution of a gene:Multiple genes for LDH isozymes provide a model of the evolution of gene structure, function and regulation. Science 189:102-114.
14.McLeod J & Wroble wski B. 1958. Lactic dehydrogenase activity in human semen. Proc Soc Exp Biol Med 99:265-267.
15.Blanco A and Zinkham W H. 1963. Lactate dehgdrogenase in human testes. Science 139:601-602.
16.Goldberg E. 1963. Lactate and malic dehydrogenase in human spermatozoa. Science 139:602-603.
17.Goldberg E. 1977. Isozymes in testes and spermatozoa, In: Isozymes: Current Topics in Biological and Medical Research. Ratazzi MS, Scandalios JG, and Whitt GS, Eds. New York, Alan R Liss, Vol. 1. pp. 79-124.
18.Hintz M and Goldberg E. 1977. Immunohistochemical localization of LDH-X during spermatogenesis in mouse testes.Dev. Biol 57:375-384.
19.Iinkham WH, Blaco A, and Clowry L. 1964. An unusual isozyme of actate dehydrogenase in mature testes: localization, outogeny and kinetic properties. Ann WY Acad Sci 121:571-588.
20.Goldberg E & Hawtrey C. 1967. The ontogeny of sperm-specific lactate dehydrogenase in mice. J Exp Zool 164:309.
21.Gavella M, Cvitkovic O & skrabalo Z .1982. Seminal plama isoenzyme LDH-X in infertile men. Andrologia 14:104.
22.Burgos de, NMG, Burgos C,Coronel CE, de camusso AB, pigini T, Blanco A. 1979. Correlation of lactate dehydrogenase isoenzyme C4 activity with the count and motility of human spermatozoa. J Repord Fertil 55:107-111.
23.Orlando C., Casano ., Caldini AL., Forti G., Barni T., Bonfanti L., serio M. 1988. Measurement of seminal LDH-X and transferrin in normal and infertile men, J Androl 9:220-223.
24.Orlando C, krausz C Foxti G, Cassano R. 1994. Simultaneous measurement of sperm LDH, LDH-X, CPK activities and ATP content in normospermic and oligozoospermic men. Int J Androl 17:13-18.
25.Casano R, Orlando C, Serio M, Fortic C. 1991. LDH and LDH-X activity in sperm from normospermic amd oligospermic men. Int J Androl 14:257-263.
26.Bishop D.W. 1962. Spermatozoa motility. American Association for the Advancement of Science, Washing ton, D.C., U.S.A. Publication No. 72.
27.Lindemann C.H.B. & Gibbons I.R. 1975. ATP-induced motility and sliding of filaments in mammalian sperm extracted with triton-x 100. J Cell Biol. 65:147-162.
28.Tombes R.M., Shapiro B.M. 1985. Metabolite channeling: a phosphorylcreatine shuttle to mediate high engergy phosphate. Transport between sperm mitochondria and tail. Cell 41:325-334.
29.Wallimann T., Moser H., Zurbriggen B., Wegman G., Eppeaberger H.M. 1986. Creatine kinase isoenzymes in spermatozoa J. Muscle Res Cell Motil 7:25-34.
30.Huszar G., Vigne L., Corrales M. 1990. Sperm creatine kinase activity in fertile and infertile oligospermic men. J Androl 11:40-46.
31.Huszar G., Vigue L. 1994. Correlation between the rate of lipid peroxidation and cellular maturity as measured by creatine kinase activity in human spermatozoa. J Androl 15:71-77.
32.Huszar G., Vigue L. 1993. Incomplete development of human spermatozoa is associated with increased creatine phosphokinase concentration and abnormal head morphology. Mol Reprod Dev 34:292-298.
33.Huszar G., Vigue L. 1990. Spermatogenesis related change in the synthesis of the creatine kinase B-type and M-type isoforms in human spermatozoa. Mol Reprod Dev 25:258-262.
34.Huszar G., Vigue L., Morshedi M. 1992. Sperm creatine phosphokinase M-isoform ratios and fertilizing potential of men : A blinded study of 84 couples treated with in vitro fertilization. Fertil steril 57:882-888.
35.Heller C.G., Clerment Y. 1964. Kinetics of the germinal epithelium in man. Recent Prog. Horm. Res. 20:545-575.
36.Reith A.D., Bernstein A. 1991. Molecular biology of the W and steel loci. Genome Anal. 3:105-133.
37.Kretser D.M., Kerr J.B. 1994. The cytology of the testis. In knobil E., Neill J.D. (eds). Physiology & Reproduction. Raven Press, New York, 1177-1290.
38.Dix D.J., Allen J.W., Collins B.W. et al. 1996. Targetted gene disruption of HSP 70-2 resultes in failed meiosis, germ cell apotosis and male infertility. Proc. Natl. Acand. Sci. USA. 93:3264-3268.
39.Maekawa M., O'Brien D.A., Allen R.L. et al. 1989. Heat Shock cognate protein (hsc 71) and related proteins in mouse spermatogenic cells. Biol. Reprod. 40:843-852.
40.McLachlan R.I., Wreford N.G., Tsonic C. et al. 1994. Testosterone effects on spermatogenesis in the gonadotrophin releasing hormone immunized rat. Biol. Reprod. 50:271-280.
41.O'Donnell L., McLachlan R.I., Wreford N.G. et al. 1994. Testosterone promotes the conversion of round spermatids between Ⅶ and Ⅷ of the rat spermatogenic cycle. Endocrinolog 135:2608-2614.
42.Perryman K.J., Stanton P.G., Loveland K.L. et al. 1996. Hormonal dependency of neural cadherin in the binding of round spermatids to sertoli cells in vitro. Endocrinology 137:3877-3883.
43.Sun Y.T., Irby D.C., Robertson D.M. et al. 1989. The effects of exogenously administered testosterone on spermatogenesis in intact and hypophysectomised rats. Endocrinology 125:2141-2149.
44.Singh J., O'Neill C., Handelsman D.J. 1995. Induction of spermatogenesis by androgens in gonadotrophin deficient (hpg) mice. Endocrinology 136:5311-5321.
45.Kumar T.R., Wang Y., Lu N. et al. 1997. Follicle stimulating hormone is required for ovarian follicle maturation but not male fertility. Nature Genet. 15:201-204.
46.Henriksen K., Kangasniemi M., Parvinen M. et al. 1996. In vitro follicle-stimulating hormone prevents apoptosis and stimulates deoxyribonucleic acid synthesis in the rat seminiferous epithelium in a stage specific fashion. Endocrinology 137:2141-2149.
47.Shetty J., Marathe G.K., Dighe R.R. 1996. Specific immunoneutralization of FSH leads to apoptotic cell death of the pachytene spermatocytes and spermatogonial cells in the rat. Endocrinology. 137:2179-2182.
48.McLachlan R.I., Wreford N.G., Kretser D.M. et al. 1995. The effects of recombinant follicle-stimulating hormone on the restoration of spermatogenesis in the gonadotrophin-releasing hormone immunized rat. Bilo. Reprod. 50:271-280.
49.Lerchl A., Sotiriadou S., Behre H.M. et al. 1993. Restoration of spermatogenesis by follicle-stimulating hormone despite low intratesticular testosterone in photo-inhibited hypogonadotrophic Djungarian hamsters (phodopus sungorus). Biol. Reprod. 49:1108-1116.
50.Aitken R.J., Gordon Baker H.W. and Irvine D.S. 1995. On the nature of semen quality and infertility. Hum. Reprod 10:248-249.
51.Barratt C.L.R. 1995. On the accuracy and clinical value of semen laboratory tests. Hum. Reprod. 10:250-252.
52.Seiblel M.M. and Zilberstein M. 1995. The diagnosis of male infertility by semen quality: the shape of sperm morphology. Hum. Reprod. 10:247-252.
53.Barratt C.L.R. , Dunphy B.C., Thomas E.J. et al. 1988. Semen characteristics of 49 fertile men. Andrologia 20:264-269.
54.Barratt C.L.R., Naeeni M., Clements S. et al. 1995. Clinical value of sperm morphology measurements for in vivo fertility:comparison between World Health Organisation (WHO) criteria of 1987 and 1992. Hum. Reprod. 10:587-593.
55.Mayaux M.J., schwatz D., Czyglik F. et al. 1985. Conception rates according to semen characteristics in a series of 15364 insemination cycles: results of a multivariate analysis. Andrologia 17:9-15.
56.Bostofte E., Bagger P., Michael A. et al. 1990. Fertility Prognosis for infertile men: results of follow up study of semen analysis in infertile men from two different populations evaluated by the Cox regression model. Fertil. Steril. 54:1100-1106.
57.Barratt C.L.R., Mcleod I. D. , Dunphy B.C. et al. 1992a. Prognostic significance of two putative sperm function tests: hypoosmotic swelling and bovine sperm mucus penetration tests ( Penetrak ) . Hum. Reprod. 7:1240-1244.
58.Campana A., Sakkas D. Stalberg A. et al. 1996. Intrauterine insemination: evaluation of the result according to the woman's age, spermquality, total sperm count per insemination and life table analysis. Hum. Reprod. 11:732-736.
59.Burr R.B., Siegberg R., Flaherty S.P. et al. 1996. The influence of sperm morphology and the number of motile sperm inseminated on the outcome of intrauterine insemination combined with mild ovarian stimulation Fertil. Steril. 65:127-132.
60.Menkveld R., Stander F.S.H., Kotze T.J.V. et al. 1990. The evaluation of morphological characteristic of human sperm spermatozoa according to stricter criteria. Hum. Reprod. 5:586-592.
61.Menkveld R., Rhemvev J.P.T., Franken D.R. et al. 1996. Acrosomal morphology as a novel criterion for male fertility diagnosis: relation with acrosin activity, morphology ( strict criteria ) , and fertilization in vitro. Fertil. Steril. 65:637-644.
62.Matson P.L. 1995. External quality control for semen analysis and sperm antibody determinatoons: report on a pilot scheme. Hum.Reprod. 10: 620-625.
63.Aitken R.J., Irvine D.S. Wu F.C. 1991. Prospective analysis of sperm-oocyte fusion and reactive oxygen species generation as criteria for the diagnosis of infertility. Am.J. Obstet. Gynecol. 164:542-551.
64.Sukcharoen N., Keith J., Irvine D.S. et al. 1995a. Predicting the fertilising potential of human sperm suspension in vitro: importance of sperm morphology and leukocyte contamination. Fertil. Steril. 63:1293-1300.
65.LeVan K.M., Goldberg E. 1991. Properties of human testis-specific lactate dehydrogenase expressed from Escherichia coli . Biochemical Journal 273:587-592.
66.Millan J.L., Driscoll C.E. LeVan K.M.& Goldberg E. 1987. Epitopes of human testis-specific lactate dehydrogenase deduced from a cDNA sequence. Proceedings of the National Academy of sciences. USA. 84:5311-5315.
67.Edwards Y.H., Povey S., LeVan K.M., Driscol C.E. Millan J.L. & Goldberg E.1987. Locus determining the human sperm-specific lactate dehydrogenase, LDHC, is syntemic with LDHA. Development Genetics 8:219-232.
68.Alcivar A.A., Transler J.M., Hake L.E., Salehi-Ashtiani, Goldberg E. & Hecht N.B. 1991. DNA methylation and expression of genes coding for lactate dehydrogenase A and C during rodent spermatogenesis. Biology of Reproduction. 44:527-535.
69.Tanaka S. & Fukumoto H. 1986. A postmeiotically expressed clone encodes lactate dehydrogenase isozyme x. Biochemical and Biophysical Research Communications 136:760-766.
70.Gerez de Burgos N.M., Burgos C., Coronel C.E. Bartarelli de Camusso A., Pigini T. & Blanco A. 1979. Correlation of lactate dehydrogenase isoenzyme C4 activity with the count ans motility of human spermatozoa. Journal of Reproduction and Fertility 55:107-111.
71.Velasco J.A.N. Zapata I.T., Hernandez P.M, Albacete M.P., Oltra J.Y. & Paricio J.J.P. 1993. Lactate dehydrogenase-C4 activity in seminal plasma and male infertility. Fertility and sterility 60:331-335.
72.Virji W. 1985. LDH-C4 in human seminal plasma and its relationship to testicular function. I. Methodological aspects. International Journal of Andrology 8:193-200.
73.Jounnet P. & David G. 1978. Evolution of the properties of semen immediately following vasectomy. Fertility and sterility. 29:435-441.
74.Eliasson R.&Virii N. 1985. LDH-C4 in human seminal plasma and its relationship to testicular function. Ⅱ. Clinical aspects. International Journal of Andrology 8:201-214.
75.Cvitkovic M., Gavella Z., Papic A., Singer A., & skrabalo Z. 1984. Seminal plasma isoeazyme LDH-X values in extreme oligozoospermia. In: The Male Factor in Human Infertility Diagnosis and Treatment. MTP presis LTD, Lancaster.
76.Mann T. & Lutwak-Mann C. 1981. Male reproductive function and semen. Springer Vevlag. New York.
77.MacLeod J. 1964. Human seminal cytology as a sensitive indicator of the germinal epithelium. International Jourmal of Fertility 9:281-295.
78.Toth A. 1979. Reversible toxic effect of salicylazo-sulphapyridine on semen quality. Fertility and sterility 31:538-540.
79.Reader S.C.J., shingles C & stonard M.D. 1991. Acute testicular toxicity of 1,3-dinitrobenzene and ethylene glycol monomethyl ether in the rat: Evaluation of biochemical effect markers and hormonal responses. Fundamental and Applied Toxicology 16:61-70.
80.Haqqi T.M. & Adhami U.M. 1982. Testicular damage and change in serum LDH isozyme pattern induced by multiple sub-lethal doses of apholate in albino rats. Toxicology letters 12:199-205.
81.Golderg E. 1987. Antigenic sites of lactate dehydrogenase-C4. Isozymes: Current Topics in Biological and Medical Research 14:103-122.
82.Tung K.S.K., Goldberg E.H & Goldberg E. 1979. Immunobiological consequence of immunization of female mice with homologous spermatozoa: Induction of infertility. Journal of Reproductive Immunology 1:145-158.
83.Naz R.K. 1987. Involvement of fertilization antigen ( FA-1 ) in involuntary immunoinfertility in humans. Journal of Clincial Investigation 80:1375-1383.
84.Huszar G., Corrales M, Vigue L. 1988a. Correlation between sperm creatine phosphokinase activity and sperm concentrations in normospermic and oligospermic men. Gamete Res 19:67-75.
85.Huszar G. 1994. The role of sperm creatine kinase in assessment of male fertily. Reprod Med Rev. 3:179-187.
86.Turner T.T. 1995. On the epididymis and its role in the development of the fertile ejaculate. J Androl 16(4): 292-298.
87.Huszar G., Patrizio P., Vigue L., Willets M., Wilker C., Adhoot D. & Johnson L. 1998. Cytoplasmic extrusion and the switch from creatine kinase B to M isoform are completed by the commencement of epididymal transport in human and stallion spermatozoa. J. Andro 19(1):11-20.
88.Word Health Organization,1992, WHO Laboratory Mannual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction. Cambridge University Press,Cambridge.
89.Gunnar S., Finn E.,Von.E., and Peter K.1984. Additional lactate dehydrogenase (LDH) isoenzymes in normal testis and spermatozoa of adult man. Mol Gen Genet. 198:172-174
90.Aitken RJ, clarkson JS. 1988. Significance of reactive oxygen species. and anti-oxidants in defining the efficancy of sperm preparation techniques. J Androl 9:367-376.
91.Gerald P. Butrimoviyz, Francis Farina, and Ira Sharlip. 1983. Micromethod for Determination of lactate dehydrogenase isoengyme C4 activity in human seminal plasma. Clin. Chem 29(8)1518-1521.
92.Gabor Huszar , Lynne Vigue. 1993. Incomplete development of human spermatozoa is associated with increased creatine phosphokinase concentration and abnormal head Morphology. Molecular Reproduction and Development 34:292-298.
93.Leon L. Gershbein and Darid R. Thielen. 1988. Enzymatic and Electrolytic profiles of Human Semen. The prostate 12:263-269.
94.R.S. Sidhu, J. Hallak, R.K. Sharma, A.J. Thomas, Jr., and A. Agarwal. 1998. Relationship between creatine kinase levels and clinical Diagnosis of infertility. Journal of Assested Reproduction and Genetics 15(4):188-192.
95.schmidt E. & schmidt F. 1974. The inportance of enzymatic analysis in medicine. In: Bergmeyer H c(ed). Methods of enzymatic analysis, 2nd Eng edn. Vol 1:6-14. Verlag chemie, weinheim.
96.Osinowa OA. 1981. Studies on leakage of enzymes from Washed bull and ram spermatozoa. J Reprod Fertil 62:549-554.
97.Harrison RAP. & White IG. 1972. Glycolytic enzymes in spermatozoa and cytoplasmic droplets of bull, boar and ram, and their leakage after shock. J Reprod Fertil 30:105.
98.Huszar G, Vigue L, corrales M. 1988b. Sperm creatine phosphokinase activity as a measure of sperm quality in normospermic, variablesper- -mic and oligospermic men. Biol Reprod 38:2061-2066.
99.Sasmira L., Nabil S., Lynne V., Marcelia C., Gabor H. 1996. Biochemical markers of early and late spermatogenesis: Relatioship between the lactate dehydrogenase-X and creatine kinase-M isoform concentrations in human spermatozoa. Mol Reprod Dev 43:495-502.
100.Aithen J., Harkiss D., Buclscigham D. 1993. Relationship between iron-catalized lipid peroxidation potential and human sperm function. J Reprod Fertil 98:257-265.
101.Aitken J., Krausz C., Buckingham D. 1994. Relationship between biochemical markers for residual sperm cytoplasma , reactive oxygen species generation, and the presence of leukocytes and precussor germ cells in human sperm suspensions . Mol Reprod Dev 39:268-279.

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