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研究生:曾素真
研究生(外文):Sue-Jen Tzeng
論文名稱:臺灣植物葉表Pantoeaagglomerans及其他黃色細菌之鑑定與特性
論文名稱(外文):Identification and Characterization of Foliar Pantoea agglomerans and Other Yellow-pigmented Bacteria from Taiwan
指導教授:曾國欽曾國欽引用關係
指導教授(外文):Kuo-Ching Tzeng
學位類別:碩士
校院名稱:國立中興大學
系所名稱:植物病理學系
學門:農業科學學門
學類:植物保護學類
論文種類:學術論文
論文出版年:2001
畢業學年度:89
語文別:中文
中文關鍵詞:Pantoea agglomerans黃色細菌葉表鑑定特性
外文關鍵詞:FoliarPantoea agglomeransYellow-pigmented BacteriaIdentificationCharacterization
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陸、中文摘要
於臺灣臺中、彰化、南投、嘉義、花蓮等地區之植物葉表分離出 175 株黃色細菌菌株,其中 106 株菌株為革蘭氏陰性細菌,其餘 69 株菌株為革蘭氏陽性細菌。以 Biolog 系統鑑定,結果得知 106 株革蘭氏陰性之葉表黃色細菌菌株中,有 61 株菌株分別屬於 Actinobacillus、Burkholderia、Chryseobacterium、Enterobacter、Erwinia、Flavimonas、Haemophilus、Pantoea、Pseudomonas、Sphingomonas 、Stenotrophomonas 及 Xanthomonas 屬之細菌;而 69 菌株革蘭氏陽性之葉表黃色細菌菌株中,有 13 株革蘭氏陽性菌株分別屬於 Corynebacterium、Curtobacterium、Kytococcus、Microbacterium、Micrococcus 與 Staphylococcus 屬之細菌。革蘭氏陰性之葉表黃色細菌菌株中,有 17 株為 Pantoea agglomerans 。以 Biolog 系統測試此 17 株 P. agglomerans 菌株對 95 種碳素源之利用情形,結果顯示,此些菌株皆可利用之碳素源有 40 種;皆無法利用的碳素源有 18 種,而其餘 37 種碳素源之利用則因菌株之不同而有所差異。此些菌株不產生過敏性反應、不引起軟腐,以對細胞分裂素 (cytokinin) 合成基因具專一性之引子對 etzⅠL / etzⅠR 進行聚合酵素連鎖反應 (polymerase chain reaction, PCR) 測試,結果得悉此些 P. agglomerans 菌株不具細胞分裂素合成基因特有之 607 bp 產物。以培養基平板法測試 P. agglomerans 菌株對臺灣常見葉部植物病原細菌生長之拮抗能力,結果顯示大部分測試之 P. agglomerans 菌株對所測試之植物病原細菌菌株具拮抗能力。將 P. agglomerans Yh 93 與 Yh 95 兩菌株分別與甜椒細菌性斑點病菌XVT 40菌株同時接種於甜椒葉片,試驗結果顯示其可減少甜椒細菌性斑點病之發生。

柒、英文摘要
A total of one hundred and seventy five strains of yellow-pigmented bacteria were isolated from twenty-two crop species and four weed plant species at different localities in Taiwan. Among them, 106 strains were Gram negative, and the other 69 strains were Gram positive. Based on Biolog identification system, 61 strains of Gram negative bacteria were identified as species in Actinobacillus, Burkholderia, Chryseobacterium, Enterobacter, Erwinia, Flavimonas, Haemophilus, Pantoea, Pseudomonas, Sphingomonas, Stenotrophomonas, and Xanthomonas. Thirteen strains of Gram positive bacteria were identified as species in Corynebacterium, Curtobacterium, Kytococcus, Microbacterium, Micrococcus, and Staphylococcus. There were 17 strains of Pantoea agglomerans isolated in this study. Utilization of carbon sources by these 17 strains of P. agglomerans were examined with the Biolog GN2 Microplate. The results revealed that among the 95 carbon sources tested, all these 17 strains could utilize 40 carbon sources; and varied in the utilization of 37 carbon sources; in addition, they all did not utilize the other 18 carbon sources. When DNAs from P. agglomerans strains isolated in this study were used as templates, no specific DNA fragment was amplified by PCR with primer pairs etzⅠL/etzⅠR, which are specific to cytokinin biosynthesis gene (etz) of P. agglomerans pv. gypsophilae. Most strains of P. agglomerans isolated showed inhibitory effect on the growth of phytopathogenic bacteria tested. The relative disease index of bacterial spot on leaves of sweet pepper co-inoculated with cell suspensions of X. axonopodis pv. vesicatoria XVT40 and P. agglomerans Yh93 or Yh95 was significantly lower than that of the control plants on which the plants were inoculated with X. axonopodis pv. vesicatoria XVT40 only.

目錄
壹、前言………………………………………………………1
貳、材料與方法………………………………………………4
一、葉表黃色細菌菌株之分離………………………………………….4
二、葉表黃色細菌菌株之鑑定………………………………………….4
三、葉表Pantoea agglomerans生理生化特性之測試………………….5
四、葉表Pantoea agglomerans細胞分裂素 (cytokinin) 合成基因
之偵測………………………………………………………………6
五、葉表黃色細菌Pantoea agglomerans對葉部植物病原細菌
拮抗能力之測試……………………………………………………8
(一)培養基上對葉部植物病原細菌之拮抗能力測試……………8
(二)碳素源對茄科植物細菌性斑點病菌拮抗能力之影響…….…9
六、葉表黃色細菌Pantoea agglomerans對甜椒細菌性斑點病
發生之影響……………………………….…………………….…9
(一)供試植物………………………………………………………9
(二)供試細菌………………………………………………………9
(三)葉表黃色細菌Pantoea agglomerans對甜椒細菌性斑點病
發生之影響……………………………..……………………10
(四)相對發病指數 (relative disease index)………………………10
參、結果……………………………………………………….11
一、葉表黃色細菌菌株之分離……………………………………….11
二、葉表黃色細菌菌株之鑑定…………………………………………11
(一)革蘭氏陰性葉表黃色細菌之鑑定…………………………11
(二)革蘭氏陽性葉表黃色細菌之鑑定………………………….12
三、葉表 Pantoea agglomerans 之生理生化特性……………………12
四、葉表 Pantoea agglomerans 對 95 種碳素源之利用…………….13
五、葉表Pantoea agglomerans細胞分裂素 (cytokinin) 合成基因
之偵測……………………………………………………………14
六、葉表Pantoea agglomerans對植物病原細菌之拮抗能力……….14
(一)培養基上對葉部植物病原細菌之拮抗能力……….………14
(二)碳素源對茄科植物細菌性斑點病菌拮抗能力之影響…….15
七、葉表黃色細菌Pantoea agglomerans對甜椒細菌性斑點病
發生之影響………………………………………………………15
肆、討論……………………………………………………….16
伍、參考文獻…………………………………………………19
陸、中文摘要…………………………………………………24
柒、英文摘要…………………………………………………25
捌、圖表……………………………………………………………….27

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