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研究生:謝旻均
研究生(外文):M.C. Hsieh
論文名稱:植物細胞分裂素對水稻幼苗生長的影響之研究
論文名稱(外文):A Study on The Effects of Cytokinin on The Growth of Rice Seedlings
指導教授:董敏生
指導教授(外文):M.S. Tung
學位類別:碩士
校院名稱:國立中興大學
系所名稱:植物學系
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2001
畢業學年度:89
語文別:中文
論文頁數:27
中文關鍵詞:植物細胞分裂素水稻
外文關鍵詞:CytokininRice
相關次數:
  • 被引用被引用:1
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中文摘要
本研究的目的主要在探討不同濃度的植物細胞分裂素及相關化學物質對水稻幼苗生長發育能力的影響。
實驗是將Ade、AMP和植物細胞分裂素(iPA, t-Z 和DHZ)與Hoagland溶液混合成低濃度(10-16到10-9M),然後測試對水稻幼苗生長發育有如何的影響。實驗結果顯示在夏季10 到10 M的Ade、10 到10 M的AMP以及10 到10 M的DHZ會增進水稻shoot的生長,但是濃度10 到10 M的t-Z有抑制的結果而iPA在10-16至10-9M皆無作用。在夏季根部的生長發育中,只有10 到10 M的Ade有效的增進根部的發育,而其他的組別皆無作用。在冬季培養的情況是濃度10 M的ade、10 到10 M 的AMP和10 M的 t-Z可增進稻苗shoot的生長,而iPA和DHZ皆無。在冬季根部的生長發育,只有10 到10 M濃度的AMP是有促進作用而其他的處理皆無。由這些結果可推測,台梗3號幼苗在夏季及冬季有不同的酵素系統來調控生長。
另在夏季以10-10M Ade,10-12及10-10M DHZ處理以幼苗shoot被發現細胞胞器(粒線體、葉綠體及細胞質)的蛋白質皆比對照組的高,且光合速率(Hill reaction activity)也比對照組的佳。 由這些結果可推知,Ade、AMP及DHZ在適當濃度下可當稻苗的營養劑。另外本研究發現Nucleotide的t-Z及DHZ比free base的效果來得好。
Ade, AMP, iPA, t-Z and DHZ each was mixed with Hoagland solution to make low concentrations ( 10 to 10 M), which were then tested on the capacity of affecting rice( Oryza sativa L cv. Tai-keng 3) seedling growth in summer and winter cultivation season in Taiwan. In summer cultivation season( May to Aug.), Ade at 10 to 10 M, AMP at 10 to 10 M and DHZ at 10 to 10 M all were effective in promoting rice shoot growth, while iPA and t-Z at 10 to 10 were ineffective and prohibitory, respectively. In root growth, only Ade at 10 to 10 M were effective, while others were inefficient. In winter cultivation season( Nov. to Feb), active compounds which promote shoot growth included Ade at 10 M, AMP at 10 to 10 M and t-Z at 10 M. Other two iPA and DHZ were ineffective. In root growth, only AMP at 10 to 10 M were effective. These results indicate that Ade and AMP are common reagents for promoting rice shoot growth of both summer and winter cultivation season and also suggest that enzyme systems in rice seedlings growing in disparate season of a year may be somehow various for synthesizing different types of cytokinin. Furthermore, analytical results of protein level in cytosol and chloroplast fraction and Hill reaction activity of isolated chloroplasts from treatments of Ade at 10 M, AMP at 10 M and DHZ at 10 M all showed higher levels than the control during summer cultivation season, suggesting that Ade, AMP and DHZ could be considered as nutrients for producing stronger rice seedlings.
In addition, results showed that nucleotide CK were better than free CK in promoting rice and lettuce seedling growth.
目錄
縮寫表…………………………………………………………1.
中文摘要………………………………………………………2.
英文摘要………………………………………………………3.
前言……………………………………………………………5.
前人研究………………………………………………………7.
材料和方法……………………………………………………9.
結果和討論…………………………………………………..15.
參考文獻……………………………………………………..23.
附錄…………………………………………………………..27.
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