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研究生:廖庭寬
研究生(外文):Liao, Ting Kuan
論文名稱:生物濾床中乙酸乙酯抑制二甲苯去除現象之研究
指導教授:李季眉李季眉引用關係
指導教授(外文):Lee, Chi Mei
學位類別:碩士
校院名稱:國立中興大學
系所名稱:環境工程學系
學門:工程學門
學類:環境工程學類
論文種類:學術論文
論文出版年:2001
畢業學年度:89
語文別:中文
論文頁數:166
中文關鍵詞:生物濾床二甲苯乙酸乙酯抑制現象菌種分離純化
外文關鍵詞:biofiliterxyleneethyl acetateRhodococcus fasciansBurkholderia norimbergensisComamonas testosteroni
相關次數:
  • 被引用被引用:10
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生物濾床處理鄰二甲苯與乙酸乙酯混合廢氣時,乙酸乙酯的存在會抑制鄰二甲苯去除,本研究從微生物角度著手尋找抑制原因。首先篩選出三株具有不同分解特性之菌株,菌株X18可分解鄰二甲苯但不具乙酸乙酯分解能力,菌株X19兼具鄰二甲苯及乙酸乙酯分解能力,而菌株Ac6可分解乙酸乙酯但無法分解鄰二甲苯。
從批次實驗結果得知菌株X18降解鄰二甲苯之趨勢不會受到乙酸乙酯影響;而菌株X19降解鄰二甲苯的速率會受到乙酸乙酯抑制而減緩,抑制之可能原因為乙酸乙酯降解後產生之乙酸及乙醇,乙酸導致pH值劇降而抑制菌株生長,而乙醇累積使鄰二甲苯的負荷提高,導致菌株X19活性降低。
從各菌株組合之實驗結果顯示,乙酸乙酯分解菌Ac6的添加有助於菌株X18降解鄰二甲苯,並且能降低菌株X19的抑制情形,而抑制鄰二甲苯降解的主要因素為質傳速率限制、鄰二甲苯之負荷、族群競爭以及乙醇的助溶效應。
將各菌株組合植入生物濾床中發現純菌組X19會因乙醇的助溶效應,使鄰二甲苯負荷增加而造成菌株活性不佳,導致乙酸乙酯及鄰二甲苯去除效率不佳,而植種乙酸乙酯分解菌Ac6可促進濾床降解鄰二甲苯及乙酸乙酯,降低因乙醇累積產生的抑制現象。
在所有植種組合中以菌株X18+Ac6混合菌組之去除效果最佳,其次為添加污泥組,可知在生物濾床中植種適當純種菌株確實有促進濾床降解鄰二甲苯的效果。
目錄 I
表目錄 V
圖目錄 VII
第一章 前言
第二章 文獻回顧
2-1 噴塗業產生之VOCs廢氣
2-2 二甲苯及乙酸乙酯之物化特性及其危害
2-3 VOCs 廢氣之控制技術
2-3-1 物化處理
2-3-2 生物處理
2-3-2-1 生物洗滌塔
2-3-2-2 生物滴濾塔
2-3-2-3 生物濾床
2-4 生物濾床設計及操作因子
2-4-1 濾料的選擇
2-4-2 濾床形式及尺寸
2-4-3 廢氣特性
2-4-4 微生物種類及特性
2-4-5 溫度控制
2-4-6 溼度控制
2-4-7 pH值
2-4-8 營養源
2-4-9 壓損及流速
2-5 在生物濾床中添加純種菌株的優點
2-6 二甲苯與乙酸乙酯之好氧生物分解
2-7 抑制機制之探討
第三章 材料與方法
3-1 研究內容
3-2 樣品來源
3-3 實驗藥品
3-3-1 碳源
3-3-2 實驗用水
3-3-3 無機營養鹽(HCMM2)
3-3-4 培養基
3-3-5 Extran 鹼洗液
3-4 具分解二甲苯及乙酸乙酯能力菌株
3-4-1 自混合族群中分離具有分解能力之菌株
3-4-1-1 馴化具分解能力之菌株
3-4-1-2 菌株之分離與純化
3-4-1-3 具分解能力菌株之篩選
3-4-2 自實驗室保存之純種菌株篩選
3-4-2-1 分解能力回復
3-4-2-2 分解能力測試
3-5 光學密度(O.D.)與細胞乾重之關係
3-6 菌株對鄰二甲苯及乙酸乙酯去除能力之批次實驗
3-6-1 VOCs 之檢量線
3-6-2 批次實驗流程
3-6-3 分析方法
3-7 菌株於不同溫度下之生長速率
3-7-1 醋酸鹽利用能力測試
3-3-6 溫度梯度實驗
3-8 顯微鏡觀察及照相
3-9 菌種鑑定
3-9-1 革蘭氏染色
3-9-2 Biolog GP/GN Microplate菌種鑑定
3-10 各種菌株組合於不同溫度下降解鄰二甲苯及乙酸乙酯之批次實驗
3-11 生物濾床實驗
3-11-1 生物濾床設備
3-11-2 濾料製備
3-11-3 植種菌種
3-11-4 操作條件
3-11-5 分析項目與方法
第四章 結果與討論
4-1 分離篩選具鄰二甲苯或乙酸乙酯分解能力之菌株
4-1-1 鄰二甲苯分解菌之篩選結果
4-1-2 乙酸乙酯分解菌之篩選結果
4-2 利用菌株的生長特性試驗結果篩選生物濾床植種菌株
4-2-1 菌株於不同基質濃度下菌株的生長情形之探討
4-2-2 鄰二甲苯與乙酸乙酯分解菌株對不同基質利用能力之探討
4-2-3 生物濾床植種菌株之篩選
4-3 利用批次實驗探討鄰二甲苯及乙酸乙酯分解菌株之基質利用情形
4-3-1 鄰二甲苯分解菌X18及X19之批次實驗結果
4-3-2 影響鄰二甲苯降解速率因子之探討
4-3-3 乙酸乙酯分解菌Ac6之批次實驗結果
4-4 鄰二甲苯與乙酸乙酯分解菌於不同溫度下之生長速率之探討
4-5 菌種鑑定結果
4-6 各種菌株組合於不同溫度下之批次實驗結果 108
4-6-1 30℃下不同菌株組合降解不同比例鄰二甲苯與乙酸乙酯之批次
實驗結果
4-6-2 18℃下不同菌株組合降解不同比例鄰二甲苯與乙酸乙酯之批次
實驗結果
4-6-3 小結
4-7 在生物濾床中植種不同組合菌株之去除率比較
4-7-1 生物濾床操作因子之控制
4-7-2 各植種組合於不同進流濃度下之處理效率
4-7-1 生物濾床之去除效率之比較
第五章 結論與建議
5-1 結論
5-2 建議
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