臺灣博碩士論文加值系統

摘要
學號：M8610006
論文名稱：薑科植物花青素標記之研究 總頁數：174
學校名稱：國立屏東科技大學 所別：熱帶農業研究所 碩士班
畢業時間及摘要別：89學年度第2學期碩士學位論文摘要
研究生：陳 禾 宗 指導教授：呂 廷 森 博士
論文摘要內容：
為瞭解薑科（Zingiberaceae）植物之花青素分布以探討花青素標記應用於薑科植物化學分類上之可行性及花色與色素組成之關係，本研究針對薑科9個屬41個種之67個樣品利用各種色層分析法與光譜分析法進行調查。結果得知anthocyanidins之種類豐富，以cyanidin(Cy)與delphinidin(Dp)分布最廣，分別佔本研究樣品的58%和54%，其次為malvidin(Mv)、peonidin(Pn)及pelargonidin(Pg)，但未發現petunidin；依組成類型可區分為9類。關於花色與色素組成之關係，紅色系主要含Cy，橘色系則含Pg或Cy，而紅紫色系主要由Dp或Mv所構成。其次依anthocyanins之組成類型亦可區分為9類，其中主要色素為花青素1(Dp 3-malonylglucoside)、2(Cy 3-rutinoside)和3(Mv 3-rutinoside)，且Dp 3-malonylglucoside則可望成為薑科於單子葉植物中之化學標記，而Mv 3-rutinoside成為Curcuma在薑科各屬間之分類標記。利用花青素標記對薑科植物進行化學分類之結果顯示，基本上在亞科和族的層級中與傳統形態分類是一致的；且薑科植物花青素組成型態亦於單子葉植物各科中呈現出其特徵性。以上結果可提供薑科植物化學分類之參考及作為新色系育種上之重要資訊。

ABSTRACT
The objective of this study is to elucidate the distribution of anthocyanin in Zingiberaceae plants and to investigate the feasibility of utilizing anthocyanin markers in chemotaxonomy. Also investigated is the relationship between the flower colors and the composition of anthocyanin. This study utilized chromatographic and spectroscopic methods to investigate the anthocyanin distribution among 67 samples of 41 species under 9 genera in Zingiberaceae. Results of the study showed that the type of anthocyanidin was varied greatly and the composition could be divided into 9 categories. Among them, cyanidin (Cy) and delphinidin (Dp) were the most widely distributed pigments at 58% and 54%, respectively. They were followed by malvidin (Mv), peonidin (Pn) and pelargonidin (Pg). Petunidin, however, has not yet to be found. Determination of the relationship between the flower colors and the composition of anthocyanidin showed that the red color series contained mainly Cy, the orange color series contained Pg or Cy, and the red-purple color series were mainly composed of Dp and Mv. On the basis of its composition, anthocyanin, too, could be divided into 9 groups. Of the 9 groups, three were considered as major pigments and they are: anthocyanin 1) Dp 3-malonyglucoside, anthocyanin 2) Cy 3-rutinoside, and anthocyanin 3) Mv 3-rutinoside. Dp 3-malonyglucoside could be used as a chemical marker for such monocotyledon plants as Zingiberaceae and Mv 3-rutinoside was considered as a chemotaxonomic marker of Curcuma among the genera of Zingiberaceae. Results of the application of anthocyanin markers in chemotaxonomy of Zingiberaceae showed that it was consistent, on the levels of subfamily and tribe, with the results of the taxonomy using traditional morphological characteristics. The composition of anthocyanin in Zingiberaceae was considered as unique characteristics among the members of monocotyledon families. The findings of this study may serve as an important reference in the chemotaxonomy of Zingiberaceae and provide valuable information for breeding flowers with new color series.
Key words: Zingiberaceae, chemotaxonomy, anthocyanin markers.

目錄
中文摘要------------------------------------------------------Ⅰ
英文摘要------------------------------------------------------Ⅱ
誌謝----------------------------------------------------------Ⅲ
目錄----------------------------------------------------------Ⅳ
圖表索引------------------------------------------------------Ⅵ
壹.前言-------------------------------------------------------1
貳.前人研究---------------------------------------------------4
一.花青素研究之現況------------------------------------------5
二.花青素標記------------------------------------------------7
參.材料與方法-------------------------------------------------11
一.植物材料--------------------------------------------------11
二.花青素組成之HPLC定性分析----------------------------------56
三.花青素之萃取、分離與純化----------------------------------57
四.花青素之TLC定性分析---------------------------------------70
五.花青素之分光光譜測定--------------------------------------70
六.花青素配醣基之TLC定性分析---------------------------------71
七.花青素之分子量測定----------------------------------------72
八.花青素之結構鑑定------------------------------------------73
肆.結果與討論-------------------------------------------------74
一.薑科植物花青素之分布--------------------------------------74
二.薑科植物花青素配醣基之分布-------------------------------104
三.薑科植物主要色素之分離、純化與鑑定-----------------------120
四.薑荷花野生種實生苗花青素之組成---------------------------148
五.結論-----------------------------------------------------154
參考文獻-----------------------------------------------------156
附錄---------------------------------------------------------168
作者簡介-----------------------------------------------------174

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