臺灣博碩士論文加值系統

本文係利用蛋白質電泳技術，分析台灣西海岸蚵岩螺（Thais clavigera）族群遺傳結構；探討地點（香山、台西、布袋、七股），成熟度（成熟、未成熟）以及時間（1999.7~2000.3、2000.11）三因子，對蚵岩螺族群遺傳結構之影響。結果顯示11個基因座中，僅Ark、Lap-1、Lap-2、Pgm-1四個為多型性基因座。地點間平均基因異質性（mean heterozygosity, H）介於0.100~0.129之間，遺傳距離（genetic distance , D）介於0.0005~0.0029之間，因此台灣西海岸蚵岩螺可視為同一族群。但族群分化現象仍然存在，分化差異是由Ark基因座造成，依族群分化差異排序為香山、布袋、台西、七股。此外，Ark基因座之異質性在四個地點都有隨著蚵岩螺體型的增大有提高的趨勢，平均基因異質性亦有相同的趨勢，而不同採樣時間對遺傳結構的影響不大。由本實驗結果推測，環境因子（環境品質和地形）和蚵岩螺生殖生態，可能是造成台灣西海岸蚵岩螺族群遺傳結構相似度高的重要因素。

The genetic structure of oyster drill Thais clavigera along the west coast of Taiwan were assayed by starch gel electrophoresis. Factors of locality（i.e. Shainsan, Taisi, Budai, Chiku）,maturity（i.e. mature, immature） and sampling time（i.e.1999.7~2000.3, 2000.11） were analyzed to evaluate their effect on drill‘s genetic structure . Four of the eleven investigated enzyme loci were polymorphic , i.e. Ark, Lap-1, Lap-2, and Pgm-1. Among the four populations , the mean heterozygosity（H）and genetic distances（D） ranged from 0.100 to 0.129 and from 0.0005 to 0.0029, respectively. Therefore, T. clavigera along the west coast of Taiwan belongs to the same population. However, differentiation among populations still existed which was contributed by Ark locus. According to the degree of differentiation, the four populations were ranked as Shainsan、Budai、Taisi and Chiku . A similar trend was also observed in the four populations, as the drill size increased, the mean heterozygosity and the heterozygosity of Ark locus increased. Sampling time had no significant effect on genetic structure of populations. It is suggested that environmental factors（e.g. environmental quality and topography）and drill’s reproductive ecology may be important in determining the genetic structure of populations.

目錄
章次…………………………………………………………………………頁次
中文摘要……………………………………………………………………Ⅲ
英文摘要……………………………………………………………………Ⅳ
謝辭…………………………………………………………………………Ⅴ
目錄…………………………………………………………………………Ⅵ
表目錄………………………………………………………………………Ⅶ
圖目錄………………………………………………………………………Ⅷ
一、 緒論……………………………………………………………………1
二、 材料與方法……………………………………………………………6
三、 結果……………………………………………………………………10
四、 討論……………………………………………………………………16
參考文獻……………………………………………………………………23
個人履歷……………………………………………………………………51

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