臺灣博碩士論文加值系統

電灑法是目前毛細管質譜儀最常用的游離方法，然而由於毛細電泳常使用的界面活性劑、緩衝溶液以及共流析的分析物，均可能在離子化過程中與分析物互相競爭而抑制質譜訊號。因此，瞭解基質對於分析物質譜訊號的影響是重要的課題，可避免毛細電泳/電灑法質譜儀定量上可能產生的問題。
選擇實驗室曾經使用毛細區帶電泳分析之西藥化合物，做為研究的分析物，改變不同靈敏度之分析物的濃度，探討共流析(coanalyte)對於目標分析物訊號的影響。由實驗得知當存在離子化效率好且濃度高的化合物會抑制分析物質譜訊號，所以有必要提高層析的解析度。本研究使用具較小的表面親和力及只需較低濃度高分子界面活性劑(poly-(sodium undecylsuflate))進行微胞電動毛細電泳的分離來提高解析度，除此之外我們以揮發性的緩衝液來製備分離系統，以達到質譜靈敏度的最佳化。

Electrospray ionization (ESI) is the most common ionization technique for interfacing capillary electrophoresis (CE) with mass spectrometer (MS). However, the ESI signal of analyte could be suppressed by the electrolytes, additives in the running buffer, and coeluted analytes. Thus, problems of low concentration detection and quantification in the CE/ESI-MS could be happened. In order to resolve the problem of low concentration detection and quantification, it is important to understand the effects of matrix on the analyte signal.
In previous work, sixteen synthetic drugs had been analysis by CE/ESI-MS. These drugs were chosen as target analytes. To study the effect of coanalyte, compounds with different ESI-MS sensitivities and concentrations were studied. The results showed that analyte with high ESI sensitivity and concentration suppressed the signals of other analytes. Thus, to avoid the effect of coanalyte, it is important to enhance the chromatographic resolution. In this study, polymeric surfactant (poly- (sodium undecylsuflate)) was found to provide better chromatographic resolution than CZE and MEKC using SDS as the surfactant.

圖目錄………………………………………………………….3
表目錄………………………………………………………….5
摘要…………………………………………………………….6
Abstract…………………………………………………………7
第一章 前言與文獻回顧
1-1 研究目的………………………………………………………8
1-2 電灑游離法的發展歷史 ……………………………………..8
1-3電灑游離法的原理 …………………………………………..11
1-4 電灑法之基質效應………………………………………….. 19
1-5 微胞電動毛細電泳的簡介…………………………………...26
1-6 毛細管電泳/質譜儀…………………………………………..31
1-7 四極式離子阱簡介 ………………………………………….35
1-8 四極式離子阱配合大氣壓界面的設計……………………...41
第二章 實驗部分
2-1 藥品…………………………………………………………...51
2-2 儀器裝置……………………………………………………...52
2-3 實驗方法……………………………………………………...53
第三章 結果與討論
3-1 基質對於質譜儀訊號影響…………………………………...58
3-1-1低濃度共流析物對分析物訊號之影響…………………59
3-1-2高濃度共流析物對分析物訊號之影響…………………59
3-1-3表面親和力對分析物訊號之影響………………………61
3-2 找尋最佳條件………………………………………………...63
3-2-1毛細管電泳之分析………………………………………63
3-2-2 鞘流溶液………………………………………………...66
3-2-3 緩衝溶液…………………………………………………68
3-3 毛細管電泳/質譜儀之分析 …………………………….….70
3-4 結論………………………………………………………….72
參考文獻………………………………………………………88

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