臺灣博碩士論文加值系統

本研究以龜紋硬皮馬勃之孢子粉接種青剛櫟苗使形成外生菌根，並與未接種者栽培1.5年後，再連同未種植苗木之相同介質，經0、10、20、40及80 ppm之鎘溶液施用500及600 ml後，比較其對鎘之截留與苗木吸收鎘之能力。
試驗結果顯示，接種之青剛櫟菌根苗雖經ANOVA分析與未接種者之鎘淋洗量並無顯著不同(p＞0.05)，但可減少淋洗液中鎘含量。而鎘在苗木地上部累積之結果顯示，接種與未接種苗木地上部鎘濃度亦無顯著差異(p＞0.05)。不過，不同鎘濃度處理卻會對淋洗液與苗木地上部鎘濃度造成顯著影響(p＜0.05)。
未接種苗木處理中，不同濃度鎘施用量與地上部Cd（r=0.875）、Na（r=0.602）之含量呈極顯著正相關(p＜0.01)，但地上部其他元素受鎘濃度處理之影響多不顯著(p＞0.05) 。苗木地上部鎘吸收量與地上部N（r=0.488）、Na（r=0.691）、Cu（r=0.449）等養分元素濃度間呈顯著正相關(p＜0.05)。
接種青剛櫟之菌根苗地上部其他元素濃度因鎘處理而有不同的表現。不同鎘施用量與地上部Cd（r=0.681）、N（r=0.576）、Na（r=0.691）濃度呈極顯著正相關(p＜0.01)；與地上部Fe（r=-0.437）濃度則呈顯著負相關(p＜0.05)。苗木地上部鎘吸收量只與地上部Na濃度間呈極顯著正相關(p＜0.01；r=0.507)。

The purpose of this study was to compare the effects of mycorrhizal and non-mycorrhizal seedlings on the interception and absorption ability of cadmium. 0, 10, 20, 40, and 80 ppm cadmium (Cd) (as CdCl2．2.5H2O solution) was added to dibbling tubes which grew 1.5-year-old mycorrhizal or non-mycorrhizal Cyclobalanopsis glauca (Thunb.) Oerst seedlings. During this study, total 500 or 600 ml cadmium chloride solution was added. The same amount of cadmium chloride solution was also added to the dibbling tubes which contained the artificial soil only.
The results showed that the content of Cd in shoot was not significantly different (p＞0.05) between inoculated seedlings and uninoculated seedlings, but the amount of Cd in leaching solution could be reduced by inoculated seedlings. The application of different Cd concentrations would significantly affect (p＜0.05) the amount of Cd in leaching solution.
The applied concentration of Cd was significantly positive related (p＜0.01) with amounts of Cd (r=0.875) and sodium (Na) (r=0.602) of shoot of uninoculated seedlings, but there was no significant relation with other elements. The Cd content of shoot of seedlings was significantly positively related (p＜0.05) with amounts of nitrogen (N) (r=0.488), Na (r=0.691) and copper (Cu) (r=0.449).
The applied concentration of Cd was significantly positively related (p＜0.01) with amounts of Cd (r=0.681), N (r=0.576) and Na (r=0.691) of shoot of inoculated seedlings, and was significantly negatively related (p＜0.05) with amounts of iron (Fe) (r=0.681). The Cd content of shoot of seedlings was significantly positively related (p＜0.05) only with amounts of Na (p＜0.01; r=0.507).
It was approved that the high concentration of Cd at 40 and 80 ppm would be harmful to the mycorrhizal development and the fungus mantle became yellowish brown.

【目錄】
摘 要----------------------------------------------------------------------------------Ⅰ
目 錄---------------------------------------------------------------------------------------Ⅳ
一、前言---------------------------------------------------------------------------- 1
二、前人研究------------------------------------------------------------------------ 3
三、材料與方法---------------------------------------------------------------------14
（一）苗木之培育---------------------------------------------------------------14
（二）鎘之施用處理-----------------------------------------------------------14
（三）淋洗液之接收與鎘含量分析-------------------------------------------15
（四）苗木地上部組織元素之養分分析-------------------------------------16
（五）試驗設計與統計分析----------------------------------------------------16
（六）試驗流程圖----------------------------------------------------------------18
四、結果------------------------------------------------------------------------19
（一）地上部乾重---------------------------------------------------------------19
（二）施鎘量500 ml經第一次淋洗之淋洗液分析-----------------------19
（三）施鎘量600 ml經第一次淋洗之淋洗液分析-----------------------21
（四）施鎘量600 ml經第二次淋洗之淋洗液分析-----------------------22
（五）僅經一次淋洗與經二次淋洗之淋洗液中鎘含量之分析比較---23
（六）僅經一次淋洗之苗木地上部鎘濃度分析---------------------------25
（七）經兩次淋洗後苗木地上部鎘濃度分析------------------------------26
（八）兩種不同淋洗方式對苗木地上部鎘濃度影響之分析比較------26
（九）苗木地上部氮(N)濃度之分析-----------------------------------------29
（十）苗木地上部磷(P)濃度之分析-----------------------------------------32
（十一）苗木地上部鉀(K)濃度之分析--------------------------------------32
（十二）苗木地上部鈣(Ca)濃度之分析-------------------------------------32
（十三）苗木地上部鎂(Mg)濃度之分析------------------------------------33
（十四）苗木地上部鈉(Na)濃度之分析------------------------------------33
（十五）苗木地上部鐵(Fe)濃度之分析-------------------------------------33
（十六）苗木地上部銅(Cu)濃度之分析-------------------------------------33
（十七）鎘施用量與無菌根青剛櫟苗地上部各元素含量之相關性---34
（十八）鎘施用量與青剛櫟菌根菌苗地上部各元素濃度之相關性---34
五、討論----------------------------------------------------------------------------38
六、結論----------------------------------------------------------------------------52
七、參考文獻--------------------------------------------------------------------53
【表目次】
頁次
表1不同接種處理苗木經施用不同鎘濃度後，其地上部乾重之分析比較-19
表2不同接種處理其施鎘量達500 ml後，經第一次淋洗之淋洗液中鎘含量分析結果-------------------------------------------------------------------------20
表3不同接種處理其施鎘量達600 ml後，經第一次淋洗之淋洗液中鎘含量分析結果------------------------------------------------------------------------21
表4不同接種處理其施鎘量達600 ml後，經第二次淋洗之淋洗液中鎘含量分析結果-------------------------------------------------------------------------23
表5未接種青剛櫟穴植管介質，僅經一次淋洗與經二次淋洗之淋洗液中鎘含量比較-------------------------------------------------------------------------24
表6接種青剛櫟苗在穴植管介質，僅經一次淋洗與經二次淋洗之淋洗液中鎘含量比較----------------------------------------------------------------------24
表7對照組穴植管介質僅經一次淋洗與經二次淋洗之淋洗液中鎘含量比較------------------------------------------------------------------------------------25
表8接種與未接種青剛櫟苗，在僅經一次淋洗後苗木地上部鎘濃度之分析比較----------------------------------------------------------------------------26
表9未接種及接種青剛櫟苗之穴植管介質經二次淋洗後，苗木地上部鎘濃度之分析比較----------------------------------------------------------------27
表10未接種青剛櫟苗僅經一次淋洗與經二次淋洗之苗木地上部鎘濃度分析比較----------------------------------------------------------------------------28
表11接種青剛櫟苗僅經一次淋洗與經二次淋洗之苗木地上部鎘濃度分析比較-------------------------------------------------------------------------------28
表12接種與未接種青剛櫟苗經5種不同濃度Cd處理後，地上部之N濃度平均值分析-----------------------------------------------------------------------29
表13接種與未接種青剛櫟苗經鎘不同濃度施用600 ml後，地上部N、P、K、Ca、Mg、Na、Fe、Cu之濃度---------------------------------------------------30
表14未接種青剛櫟苗經不同鎘濃度處理後，其施用量及鎘吸收量與地上部養分含量之關係----------------------------------------------------------------36
表15接種青剛櫟苗經不同鎘濃度處理後，其施用量及鎘吸收量與地上部養分含量之關係-------------------------------------------------------------------37
表16青剛櫟苗經不同鎘濃度處理後，其施用量及鎘吸收量與地上部養分濃度之關係--------------------------------------------------------------------------52
【圖目次】
頁次
圖1龜紋硬皮馬勃子實體之外觀--------------------------------------------------- 3
圖2苗木試驗時之示意圖------------------------------------------------------------14
圖3苗木於溫室之擺設情況---------------------------------------------------------15
圖4經施用不同鎘濃度處理後，未接種及接種青剛櫟苗木地上部乾重之比較-------------------------------------------------------------------------------39
圖5不同鎘濃度處理對苗木地上部氮濃度之影響-------------------------------45
圖6青剛櫟接種龜紋硬皮馬勃未施用鎘之正常白色菌根--------------------50
圖7青剛櫟菌根在40 ppm鎘處理受毒害的情形---------------------------50
圖8青剛櫟菌根在80 ppm鎘處理受毒害的情形---------------------------51

七、參考文獻
林瑞進 2000 青剛櫟外生菌根苗在貧瘠造林地造林之研究 國立台灣大學森林學研究所碩士論文 67頁。
黃增泉 1993 植物分類學：臺灣維管束植物科誌 國立編譯館 661頁。
胡弘道 1988 森林土壤學 國立編譯館 435頁。
胡弘道 1996 青剛櫟與美味牛肝菌之菌根純合成及共生後對苗木生長之影響 臺大農學院研究報告 36(4):329-341。
胡弘道 1999b 青剛櫟與大環柄菇、龜紋硬皮馬勃之純合成及其對青剛櫟幼苗在石礫褐壤之生長效應 行政院國家科學委員會專題研究計畫成果報告 8頁。
徐秀鳳 1993 土壤施用重金屬鎘、銅容許量之推估及以LEACHM模式模擬鎘、銅於土壤之移動 國立台灣大學環境工程學研究所碩士論文 104頁。
張添晉 1993 台北市土壤中四五級重金屬含量調查研究 台北市政府環境保護局 94頁。
張尊國 1990 以螯合劑淋洗受污染土壤時重金屬移動之研究 行政院國家科學委員會專題研究計畫成果 102頁。
張尊國 1996 台灣地區土壤重金屬污染場址鑑識（Ⅰ） 行政院國家科學委員會專題研究計畫成果報告 102頁。
莊榮輝 1996 重金屬鎘在細胞內的代謝隔離機制 行政院國家科學委員會專題研究計畫成果報告 106頁。
郭登志，黃益田 1992 鎘污染土栽培之莧菜品種對鎘吸收之效益 植物保護學會會刊 34:405-415。
陳勝利 1994 鎘對水稻幼苗與切離葉片生理之影響 國立台灣大學農藝學研究所碩士論文 80頁。
陳尊賢、駱尚廉、吳先琪 1994 桃園地區鎘污染農業土壤之綜合性再分析與評估 期末報告 行政院科技顧問組委託計畫報告 153頁。
劉棠瑞 1960 台灣木本植物圖誌上卷 國立台灣大學農學院 702頁。
鄭玉苹 1998 半純合成與自然感染青剛櫟白色外生菌根形態之比較 國立臺灣大學森林學研究所碩士論文 93頁。
盧國新 1997 化學復育法對污染土壤重金屬溶出及作物吸收重金屬之影響 國立台灣大學農業化學研究所碩士論文 102頁。
應紹舜 1996 台灣高等植物彩色圖誌第三卷（增訂一版） 應紹舜 670頁。
簡秀芳 1999 過量鎘對水稻切離葉片生理作用影響之研究 國立台灣大學農藝學研究所碩士論文 72頁。
Alloway, B. J. 1990. Heavy Metals in Soils. Halsted Press, New York. pp. 339.
Assche, F. V., and H. Clijsters. 1990. Commissioned review: Effects of metals on enzyme activity in plants. Plant Cell Environ 13:195-206.
Baker A. J. M. 1987. Metal tolerance. New Phytol. 106:93-111.
Brown, M. T., and D. A. Wilkins. 1985. Zinc tolerance of mycorrhizal Betula. New Phytol. 99:101-106.
Castellano, M. A. 1994. Current status of outplanting studies using ectomycorrhiza-inoculated forest trees. In: Mycorrhizae and Plant Health. St. Paul. Monnesota. pp. 261-281.
Chiu, S. W., Y. H. Chan, S. C. Law, K. T. Cheung, and D. Moore. 1998. Cadmium and manganese in contrast to calcium reduce yield and nutritional values of the edible mushroom Pleurotus pulmonarius. Mycol. Res. 102(4):449-457.
Colpaert, J. V., and J. A. V. Assche. 1992a. Zinc toxicity in ectomycorrhizal Pinus sylvestris. Plant Soil 143:201-211.
Colpaert, J. V., and J. A. V. Assche. 1992b. The effects of cadmium and the cadmium-zinc Interaction on the axenic growth of ectomycorrhizal fungi. Plant Soil 145:237-243.
Colpaert, J. V., and J. A. V. Assche. 1993. The effect of cadmium on ectomycorrhizal Pinus sylvstris L.. New Phytol. 123:325-333.
Costa, G., J. C. Michaut, and J. L. Morel. 1994. Influence of cadmium on water relations and gas exchanges, in phosphorus deficient Lupinus albus. Plant Physiol. Biochem. 32:105-114.
Cutler, J. M., and D. W. Rains. 1974. Characterization of cadmium uptake by plant tissue. Plant Physiol. 54:67-71.
Dixon, R. K., and C. A. Buschena. 1988. Response of ectomycorrhizal Pinus banksiana and Picea glauca to heavy metals in soil. Plant Soil 105:265-271.
Fett, J. P., J. Cambraia, M. A. Oliva, and C. P. Jordao. 1994. Cadmium uptake and growth inhibition in water hyacinths: Effects of nutrient solution factors. J. Plant Nutr. 17:1205-1217.
Fujita, M., and T. Kawanishi. 1986. Purification and characterization of a Cd-binding complex from the root tissue of water hyacinth in a Cd++ containing medium. Plant Cell Physiol. 28:379-382.
Gadd G. M. 1993. Transley review no 47. Interactions of fungi with toxic metals. New Phytol. 124:25-60.
Galli, U., H. Schuepp, and C. Brunold. 1994. Heavy metal binding by mycorrhizal fungi. Physiol. Plant. 92:364-368.
Galli, U., M. Meier, and C. Brunold. 1993. Effects of cadmium on non-mycorrhizal and mycorrhizal Norway spruce seedlings(Picea abies (L.) Karst) and its ectomycorrhizal fungus Laccaria laccata(Scop. ex Fr.)Bk. And Br.：sulphate reduction, thiols and distribution of the heavy metal. New Phytol. 125:837-843.
Godbold D. L., G. Jentschke, S. Winter, and P. Marschner. 1998. Ectomycorrhizas and amelioration of metal stress in forest trees. Chemosphere 36(4/5):757-762.
Gouia, H., M. H. Ghorbal, and C. Meyer. 2000. Effects of cadmium on activity of nitrate reductase and on other enzymes of the nitrate assimilation pathway in bean. Plant Physiol. Biochem. 38:629-638.
Grager, M., and G. Bertell. 1992. Effects of Ca2+ and Cd2+ on the carbohydrate metabolism in sugar beet(Beta vulgaris). J. Exp. Bot. 43(247):167-173.
Greger, M., and S. Lingberg. 1987. Effects of Cd2+ and EDTA on young sugar beet(Beta vulgaris).Ⅱ--Net uptake and distribution of Mg2+, Ca2+, and Fe2+ / Fe3+. Physiol. Plant. 69:81-86.
Greger, M., E. Brammer, S. Lindberg, G. Larsson and, J. Idestam-Almquist. 1991. Uptake and physiological effects of cadmium in sugar beet(Beta vulgaris) related to mineral provision. J. Exp. Bot. 42:729-737.
Hardiman, R. T., and B. Jacoby. 1984. Absorption and translocation of Cd in bush beans(Phaseolus vulgaris). Physiol. Plant. 61:670-674.
Hart, J. J., R. M. Welch, W. A. Norvell, L. A. Sullivan, and L. V. Kochian. 1998. Characterization of cadmium binding, uptake, and translocation in intact seedlings of bread and durum wheat cultivars. Plant Physiol. 116:1413-1420.
Hartley, J., J. W. G. Cairney, and A. A. Meharg. 1999. Cross-colonization of Scots pine(Pinus sylvestris) seedlings by the ectomycorrhizal fungus Paxillus involutus in the presence of inhibitory levels of Cd and Zn. New Phytol. 142:141-149.
Hartley, J., J. W. G. Cairney, F. E. Sanders, and A. A. Meharg. 1997. Toxic interactions of metal ions(Cd2+, Pb2+, Zn2+ and Sb3-) on in vitro biomass production of ectomycorrhizal fungi. New Phytol. 137:551-562.
Hernández, L. E., E. Lozano-Rodríguez, A. Gárate, and R. Carpena-Ruiz. 1998. Influnce of cadmium on the uptake, tissue accumulation and subcellular distribution of manganese in pea seedlings. Plant Sci. 132:139-151.
Holm, P. E., T. H. Christensen, S. E. Lorenz, R. E. Hamon, H. C. Domingues, E. M. Sequeira, and S. P. Mcgrath. 1998. Measured soil water concentrations of cadmium and zinc in plant pots and estimated leaching outflows from contaminated soils. Water Air Soil Pollut. 102:105-115.
Huthinson, J. J., S. D. Young, S. P. Mcgrath, H. M. West, C. R. Black, and A. J. M. Baker. 2000. Determining uptake of “non-labile” soil cadmium by Thlaspi caerulescens using isotopic dilution techniques. New Phytol. 146:453-460.
Joner, E. J., and C. Leyval. 1997. Uptake of 109Cd by roots and hyphae of a Glomus mosseae / Trifolium subterraneum mycorrhiza from soil amended with high and low concentrations of cadmium. New Phytol. 135:353-360.
Jones, M. D., and T. C. Hutchinson. 1986. The effect of mycorrhizal infection on the response of Betula papyrifera to nickel and copper. New Phytol. 102:429-442.
Kabata-Pendias, A., and H. Pendias. 1984. Trace Elements in Soil and Plants. CRC Press, Florida. pp. 315.
Khan, S., and N. N. Khan. 1983. Influence of lead and cadmium on the growth and nutrient concentration of tomato(Lycopersicon esculentum) and egg-plant(Solanum melongena). Plant Soil 74:387-394.
Leita, L., M. Contin, and A. Maggioni. 1991. Distribution of cadmium and induced Cd-binding proteins in roots, stems and leaves of Phaseolus vulgaris. Plant Sci. 77:139-147.
Lindberg, S., and G. Wingstrand. 1985. Mechanism for Cd2+ inhibition of (K+ + Mg2+) ATPase activity and K+ (86Rb+) uptake in roots of sugar beet(Beta vulgaris). Physiol. Plant. 63:181-186.
Mccreight, J. D. and D. B. Schroeder. 1982. Inhibition of growth of ectomycorrhizal fungi by cadmium, lead, nickel in vitro. Environ. Exp. Bot 22:1-7.
Meharg, A. A. 1993. The role of the plasmalemma in metal tolerance in angiosperms. Physiol. Plant. 88:1407-1410.
Morselt, A. F. W., W. T. M. Smits, and T. Limonard. 1986. Histochemical demonstration of heavy tolerance in ectomycorrhizal fungi. Plant Soil 96:417-420.
Ouariti, O., H. Gouia, and M. H. Ghorbal. 1997. Responses of bean and tomato plants to cadmium: Growth, mineral nutrition, and nitrate reduction. Plant Physiol. Biochem. 35:347-354.
Page, A. L., F. T. Bingham, and A. C. Chang. 1981. Cadmium. In: Effects of Heavy Metal Pollution in Plants, Vol.1, by N. W. Lepp, (ed.) p.77-109. Applied Sci., UK.
Schleģel, H., D. L. Godbold and A. Hüttermann. 1987. Whole plant aspects of heavy metal induced changes in CO2 uptake and water relations of spruce (Picea abies) seedlings. Physiol. Plant. 69:265-270.
Sela, M., E. Tel-or, E. Fritz, and A. Huttermann. 1988. Localization and toxic effects of cadmium, copper and uranium in azolla. Plant Physiol. 88:30-36.
Senden, M. H. M. N., F. J. M. Van Paassem, A. J. G. M. Van Der Meer, and H. Th. Wolterbeek. 1992. Cadmium-citric acid-xylem cell wall interactions in tomato plants. Plant Cell Environ. 15:71-79.
Strange, J., and M. R. Macnair. 1991. Evidence for a role for the cell membrance in copper tolerance of Mimulu guttatus. New Phytol. 119:383-388.
Thornton, I. 1981. Geochemical aspects of the distribution and forms of heavy metals in soils. In: Effect of heavy metal pollution in plants, vol. 2, N. W. Lepp,(ed.), p.1-33. Applied Sci., UK.
Trivedi, S., and L. Erdei. 1992. Effects of cadmium and lead on the accumulation of Cd2+ and K+ and on the influx and translocation of K+ in wheat of low and high K+ status. Physiol. Plant. 84:94-100.
Turnau, K., I. Kottke, J. Dexheimer, and B. Botton. 1994. Element distribution in mycelium of Pisolithus arrhizus treated with cadmium dust. Ann. Bot 74:137-142.
Vögeli-Lange, R., and G. J. Wagner. 1996. Relationship between cadmium, glutathione and cadmium-binding peptides(phytochelatins) in leaves of intact tobacco seedlings. Plant Sci. 114:11-18.
Weissenhorn, I., and C. Leyval. 1995. Root colonization of maize by a Cd-sensitive and a Cd-tolerant Glomus Mosseae and cadmium uptake in sand culture. Plant Soil 175:233-238.