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研究生:林秋宏
研究生(外文):Chiou-Hong Lin
論文名稱:綠竹筍蔗糖合成脢cDNA之選殖與檢定
論文名稱(外文):Cloning and Characterization of Sucrose Synthase cDNA from Shoots of Bamboo Leleba oldhami
指導教授:王愛玉
指導教授(外文):Ai-Yu Wang, Ph. D.
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:農業化學研究所
學門:農業科學學門
學類:農業化學類
論文種類:學術論文
論文出版年:2001
畢業學年度:89
語文別:中文
論文頁數:127
中文關鍵詞:Sucrose synthasecDNANorthern blotcDNA librarylambda ZAPIIBamboo shootsspecific probeRT-PCR
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  • 被引用被引用:21
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摘 要
蔗糖合成催化蔗糖與UDP形成果糖與UDPG的反應,後者即為多醣類生合成之前體。為了了解蔗糖合成在快速生長的竹筍中扮演的角色,因此本論文之目的即為進行綠竹筍蔗糖合成cDNA的選殖與檢定。以含有植物蔗糖合成 保守性序列的RT-PCR產物為探針,篩選綠竹筍cDNA庫,得到四種不同型式的蔗糖合成cDNA,分別為BSus1a、BSus1b、BSus2a以及BSus2b。BSus1a、BSus1b cDNA推衍出的胺基酸序列皆為816個胺基酸,分子量分別為92.83、92.98 kDa;與其它植物之Sus1-type蔗糖合成胺基酸序列的同質性最高,為90~96%之間。BSus2a及BSus2b cDNA推衍出的胺基酸序列皆為808個胺基酸,分子量分別為92.25、92.12 kDa;與Sus2-type蔗糖合成胺基酸序列的同質性最高,為89~92%之間。BSus1a與BSus1b mRNA在葉中的含量高於竹筍 (shoots),而BSus2a及BSus2b mRNA則主要存在於竹筍中。以pTrcHisA為表現載體,將四種BSus cDNA胺基酸轉譯區的序列轉形至大腸桿菌TOP10中表現。得到的重組蛋白質具有蔗糖合成的酵素活性,可被抗水稻蔗糖合成之多源性抗體辨認。由上述之結果證實了四種不同型式的cDNA,確實為綠竹筍蔗糖合成之cDNA。
Abstract
Sucrose synthase catalyzes a reaction of sucrose and UDP to form fructose and UDPG, the latter being a precursor of complex saccharide biosynthesis. The objective of this thesis is to clone and characterize the cDNA encoding sucrose synthase in the shoots of bamboo in order to understand the roles played by sucrose synthase in this rapid-growing plant. A cDNA library constructed from poly(A)+RNA of green bamboo shoots was screened for sucrose synthase by using the RT-PCR product, containing the conserved sequence of plant sucrose synthase, as a probe. Four different types of sucrose synthase cDNA, BSus1a, BSus1b, BSus2a and BSus2b were identified. BSus1a and BSus1b both encode polypeptides of 816 amino acids with relative molecular masses of 92.83 and 92.98 kDa and have highest amino acid sequence homology with Sus1-type sucrose synthase of other plants (90~96% identity). BSus2a and BSus2b both encode polypeptides of 808 amino acids with relative molecular masses of 92.25 and 92.12 kDa and have highest amino acid sequence homology with Sus2-type sucrose synthase (89~92% identity). BSus1a and BSus1b mRNA were detected at higher level in leaves than in shoots while BSus2a and BSus2b mRNA were detected predominantly in shoots. The coding region of each BSus cDNA was subcloned into an expression vector, pTrcHis A. The constructed expression plasmids were then transformed into E.coli TOP10 for expression. The recombinant proteins possessed sucrose synthase activity and showed cross-reaction to a polyclonal antibody against rice sucrose synthase. These results prove conclusively that four cDNAs encode sucrose synthase in green bamboo shoots.
目 錄
目錄……………………………………………………………………………Ⅰ
縮寫表………………………………………………………………………..Ⅲ
摘要…………………………………………………………………………..Ⅴ
Abstract…………………………………………………………………….Ⅵ
第一章 緒論………………………………………………………………….1
1 蔗糖合成之生化特性及生理角色……………………………………..1
1.1 蔗糖合成之生化特性……………………………………………..1
1.2 蔗糖合成之生理角色……………………………………………..3
1.2.1 蔗糖合成在澱粉合成上扮演之角色……………………..3
1.2.2 蔗糖合成在纖維素合成上扮演之角色…………………..4
1.2.3 蔗糖合成可能扮演之其它角色…………………………..5
2 蔗糖合成之基因與基因表現…………………………………………..6
3 本實驗室在水稻蔗糖合成的研究基礎………………………………..8
4 本論文之研究目的與方向………………………………………………..9
4.1 研究動機與目的………………………………………………………9
4.2 本論文之研究重點……………………………………………….11
第二章 材料與方法…………………………………………………………12
1 實驗材料料………………………………………………………………12
1.1 植物材料…………………………………………………………….12
1.2 載體………………………………………………………………….12
1.3 大腸桿菌菌株…………………………………………………….12
2 實驗藥品…………………………………………………………………13
2.1 一般化學試劑……………………………………………………….13
2.2 限制及核酸修飾酵素…………………………………………….13
2.3 培養基……………………………………………………………….13
2.4 放射性藥品………………………………………………………….13
3 重要儀器設備……………………………………………………………14
4 實驗方法…………………………………………………………………15
4.1 RNA之抽取及分析方法…………………………………………….15
4.2 DNA之抽取及分析方法…………………………………………….20
4.3 cDNA的合成………………………………………………………...24
4.4 cDNA庫的建立……………………………………………………...26
4.5 cDNA庫之篩選……………………………………………………...28
4.6 表現載體之建構…………………………………………………….30
4.7 重組蛋白質之檢定…………………………………………………32
4.8 放射性探針之製備…………………………………………………37
4.9 其他重要實驗方法…………………………………………………39
4.10 序列分析…………………………………………………………..44
第三章 結果與討論………………………………………………………...45
1 綠竹筍RNA的抽取……………………………………………………..45
1.1 RNA之抽取與poly(A)+RNA之純化…………………………..45
1.2 RNA之完整性分析…………………………………………...45
2 以RT-PCR進行綠竹筍蔗糖合成cDNA之選殖……………………….46
2.1 部分Sus cDNA之選殖……………………………………………...46
2.2 5''端與3''端片段之選殖………………………………….……...47
2.3 RT-PCR選殖方法之檢討……………………………………………49
3 全長Sus cDNA之選殖…………………………………………………..49
3.1綠竹筍cDNA庫的建立……………………………………………..49
3.2 cDNA庫之篩選…………...…………………………………………50
3.3正反應株的鑑定……………………………………………………..50
4 Sus cDNA序列分析……………………………………………………...51
4.1 cDNA序列及其推衍出之胺基酸序列……………………………...51
4.2 綠竹筍之Sus異構基因……………………………………………..53
4.3 3''端與5''端不轉譯區…………………………………………....54
4.4 與RT-PCR結果之比對……………………………………………..55
5 綠竹筍SuS基因表現與拷貝數目的探討………………………………55
5.1 專一性探針的製備………………………………………………….55
5.2 綠竹筍不同組織蔗糖合成基因表現的探討…………………….56
5.3 BSus異構基因之表現與異構之關係…………………………….57
5.4綠竹筍蔗糖合成基因拷貝數目分析……………………………..58
6 重組蛋白質之檢定………………………………………………………59
6.1 表現載體之建構…………………………………………………….59
6.2 重組蛋白質表現之探討…………………………………………….60
第四章 結論與展望………………………………………………………...62
參考文獻…………………………………………………………………….65
圖與表……………………………………………………………………….72
參 考 文 獻
Alwine, J.C., Kemp, D.J. and Stark, G.R. (1977) Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl paper and hybridization with DNA probes. Proc. Natl. Acad. Sci. USA 74, 5350- 5354.
Amor, Y., Haigler, C.H., Johnson, S., Wainscott, M. and Delmer, D.P. (1995) A membrane-associated form of sucrose synthase and its potential role in synthesis of cellulose and callose in plants. Proc. Natl. Acad. Sci. USA 92, 9353-9357.
Arai, M., Mori, H. and Imaseki, H. (1992) Expression of the gene for sucrose synthase during growth of mung bean seedlings. Plant Cell Physiol. 33, 503-506.
Birnboim, H.C. and Doly, J.A. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucl. Acids Res. 7, 1513-1523.
Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
Cardini, C.E., Leloir, L.F. and Chiriboga, J. (1995) The biosynthesis of sucrose. J. Biol. Chem. 214, 149-155.
Carlson, S.J. and Chourey, P.S. (1996) Evidence for plasma membrane-associated forms of sucrose synthase in maize. Mol. Gen. Genet. 252, 303-310.
Chan, H.Y., Ling, T.Y., Juang, R.H., Ting, I.N., Sung., H.Y. and Su, J.C. (1990) Sucrose synthase in rice plants. Plant Physiol. 94, 1456-1461.
Chan, M.T. and Yu, S.M. (1998) The 3'' untranslated region of rice α-amylase gene functions as a sugar-dependent mRNA stability determinant. Proc. Natl. Acad. Sci. USA 95, 6543-6547.
Chen, Y.C. and Chourey, P.S. (1989) Spatial and temporal expression of the two sucrose synthase genes in maize: Immunohistological evidence. Theor. Appl. Genet. 78, 553-559.
Cheng, W.H., Taliercio, E.W. and Chourey, P.S. (1999) Sugars modulate an unusual mode of control of cell-wall invertase gene (Incw 1) through its 3'' untranslated region in a cell suspension culture of maize. Proc. Natl. Acad. Sci. USA 96, 10512-10517.
Chomczynski, T. and Sacchi, N. (1987) Single-step of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156-159.
Chopra, S., Del favero, J., Dolferus, R. and Jacobs, M. (1992) Sucrose synthase of Arabidopsis: genomic cloning and sequence characterization. Plant Mol. Biol. 18, 131-134.
Chourey, P.S. and Nelson, O.E. (1976) The enzymatic deficiency conditioned by the shrunken-1 mutation in maize. Biochem. Genet. 14, 1041-1055.
Chourey, P.S., Latham, M. and Still, P.E. (1986) Expression of two sucrose synthetase genes in endosperm and seedling cells of maize: evidence of tissue specific polymerization of promoters. Mol. Gen. Gent. 103, 251-255.
Chourey, P.S., DeRobertis, G.A. and Still, P.E. (1988) Altered tissue specificity of the revertant shrunken allele upon Ds excision is associated with loss of expression and molecular rearrangement at the corresponding non-allelic isozyme locus in maize. Mol. Gen. Genet. 214, 300-306.
Chourey, P.S., Taliercio, E.W. and Kane, E.J. (1991) Tissue-specific expression and anaerobically induced post-transcripitional modulation of sucrose synthase gene in Sorghum bicolor M. Plant Physiol. 96, 485-490.
Chourey, P.S. Chen, Y.C. and Miller, M.E. (1991) Early degeneration in developing endosperm is unique to the shrunken mutation in maize. Maydica 36, 141-146.
Chourey, P.S., Taliercio, E.W., Carlson, S.J. Ruan, Y.-L. (1998) Genetic evidence that the two isozymes of sucrose synthase present in developing maize endosperm are critical, one for cell wall integrity and the other for starch biosynthesis. Mol. Gen. Genet. 259, 88-96.
D’Aoust, M.-A., Yelle, S. and Nguyen-Quoc, B. (1999) Antisense inhibition of tomato fruit sucrose synthase decreases fruit setting and the sucrose unloading capacity of young fruit. Plant Cell 11, 2407-2418.
Déjardin, A., Sokolov, L.N. and Kleczkowski, L.A. (1999) Sugar/osmoticum levels modulate differential abscisic acid-independent expression of two stress-responsive sucrose synthase genes in Arabidopsis. Biochem. J. 344, 503-509.
Delmer, D.P. and Amor, Y. (1995) Cellulose biosynthesis. Plant Cell 7, 987-1000.
Dretzen, G., Bellard, M., Sassone-Corsi, P. and Chambon, P. (1981) A reliable method for the recovery of DNA fragments from agarose and acrylamide gels. Anal. Biochem. 112,295-298.
Echt, C.S. and Chourey, P.S. (1985) A comparison of two sucrose synthase isozymes from normal and shrunken-1 maize. Plant Physiol. 79, 530-536.
Fu, H., Kim, S.Y. and Park, W.D. (1995) High-level tuber expression and sucrose inducibility of a potato Sus4 sucrose synthase gene require 5'' and 3'' flanking sequences and the leader intron. Plant Cell 7, 1387-1394.
Fu, H., Kim, S.Y. and Park, W.D. (1995) A potato Sus3 sucrose synthase gene contains a context-dependent 3'' element and a leader intron with both positive and negative tissue-specific effects. Plant Cell 7, 1395-1403.
Fu, H. and Park, W.D. (1995) Sink- and vascular-associated sucrose synthase functions are encoded by different gene classes in potato. Plant Cell 7, 1369-1385.
Geigenberger, P. and Stitt, M. (1993) Sucrose synthase catalyses a readily reversible reaction in vivo in developing potato tubers and other plant tissues. Planta 189, 329-339.
Gross, K.C. and Pharr, D.M. (1982) Cucumber fruit sucrose synthase isozymes. Phytochemistry 21, 1241-1244.
Hanahan, D. (1985) ”Techniques for transformation of E. coli.” in DNA Cloning, Vol. Ⅰ: A Practical Approach, Glover, D.(ed), IRL Press, Oxford, pp. 103-109.
Heim, U., Weber, H., Baumlein, H. and Wobus, U. (1993) A sucrose synthase gene of Vicia faba : Expression pattern in developing seeds in relation to starch synthesis and metabolic regulation. Planta 191, 394-401.
Hisajima, S. and Ito, T. (1981) Purification and properties of sucrose synthase from morning-glory callus cells. Biol. Plant 23, 356-364.
Huang, J.W., Chen, J.T., Yu, W.P., Shyur, L.F., Wang, A.Y., Sung, H.Y., Lee, P.D. and Su, J.C. (1996) Complete structure of three rice sucrose synthase isogenes and differential regulation of their expressions. Biosci. Biotech. Biochem. 60, 233-239.
Huang, D.Y. and Wang, A.Y. (1998) Purification and characterization of sucrose synthase isozymes from etiolated rice seedlings. Biochem. Mol. Biol. Int. 46, 107-113.
Huber, S.C., Huber, J.L., Liao, P.C., Gage, D.A., McMichael, R.W. Jr., Chourey, P.S., Hannah, L.C. and Koch, K. (1996) Phosphorylation of serine-15 of maize leaf sucrose synthase. Occurrence in vivo and possible regulatory significance. Plant Physiol. 112, 793-802.
Kleczkowski, -L.A. (1994) Glucose activation and metabolism through UDP-Glucose pyrophosphorylase in plants. Phytochemistry 37, 1507-1515.
Koch, K. E., Notle, K. D., Duke, E. R., McCarty, D. R. and Avigne, W. T. (1992) Sugar levels modulate differential expression of maize sucrose synthase genes. Plant Cell 4, 59-69.
Koch, K.E. (1996) Carbohydrate-modulated gene expression in plant. Annu. Rev. Plant Physiol. Plant Mol. Biol. 47, 509-540.
Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 27, 680-685.
Larsen, A.E., Salerno, G.L. and Pontis, H.G. (1985) Sucrose synthase from wheat leaves. Comparison with the wheat germ enzyme. Physiol. Plant. 67, 37-42.
Lehrach, H., Diamond, D., Wozney, J.M. and Boedtker, H. (1977) RNA molecular weight determination by gel electrophoresis under denaturing condition, a critical reexamination. Biochemistry 16, 4743-4751.
Logemann, J., Schell, J. and Willmitzer, L. (1987) Improved method for the isolation of RNA from plant tissues. Anal. Biochem. 163, 16-20.
Maraña, C., Garcia-Olmedo, F. and Carbonero, P. (1988). Linked sucrose synthase genes in group-7 chromosomes in hexaploid wheat (Triticum aestivum L.). Gene 63, 253-260.
Maraña, C., Garcia-Olmedo, F. and Carbonero, P. (1990). Differential expression of two types of sucrose synthase-encoding genes in wheat in response to anaerobiosis, cold shock and light. Gene 88, 167-172.
McCarty, D.R., Shaw, J.R. and Hannah, L.C. (1986) The cloning, genetic mapping, and expression of the constitutive sucrose synthase locus of maize. Proc. Natl. Acad. Sci. USA. 83, 9099-9103.
McDonell, M.W., Simon, M.N. and Studier, F.W. (1977) Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J. Mol. Biol. 110, 119-146.
Moriguchi, T. and Yamaki, S. (1988) Purification and charaterization of sucrose synthase from peach (Prunus persci) fruit. Plant Cell Physiol. 29, 1361-1366.
Murray, M.G. and Thompson, W.F. (1980) Rapid isolation of high-molecular-weight plant DNA. Nucl. Acids Res. 8, 4321-4325.
Nakai, T., Konishi, T., Zhang, X.Q., Chollet, R., Tonouchi, N., Tsuchida, T., Yoshinaga, F., Mori, H., Sakai, F. and Hayashi, T. (1998) An increase in apparent affinity for sucrose of mung bean sucrose synthase is caused by in vitro phosphorylation or directed mutagenesis of Ser11. Plant Cell Physiol. 39, 1337-1341.
Nakai, T., Tonouchi, N., Konishi, T., Kojima, Y., Tsuchida, T., Yoshinaga, F., Sakai, F. and Hayashi, T. (1999) Enhancement of cellulose production by expression of sucrose synthase in Acetobacter xylinum. Proc. Natl. Acad. Sci. USA 96, 14-18.
Nelson, N. (1994) A Photometric adaption of Somogi method for the determination of glucose. J. Biol. Chem. 153, 375-380.
Nguyen-Quoc, B., Krivitzky, M., Huber, S. C. and Lecharny, A. (1990) Sucrose synthase in developing maize leaves. Plant Physiol. 94, 516-523.
Notle, K.D. and Koch, K.E. (1993) Companion-cell specific localization of sucrose synthase in zones of phloem loading and unloading. Plant Physiol. 101, 899-905.
Pozueta-Romero, J., Yamaguchi, J., and Akazawa, T. (1991) ADPG formation by the ADP-specific cleavage of sucrose-reassessment of sucrose synthase. FEBS Lett. 291, 233-237.
Ruan, Y.L. and Chourey, P.S. (1998) A fiberless seed mutation in cotton is associated with lack of fiber cell initiation in ovule epidermis and alterations in sucrose synthase expression and carbon partitioning in developing seeds. Plant Physiol. 118, 399-406.
Sanchez de la Hoz, P., Vicente-Carbajosa, J., Mena, M. and Carbonero, P. (1992) Homologous sucrose synthase genes in barley (Hordeum vulgare) are located in chromosomes 7H (syn. 1) and 2H. FEBS Lett. 310, 46-50.
Salanoubat, M. and Belliard, G. (1987) The steady-state level of potato sucrose synthase mRNA is dependent on wounding, anaerobiosis and sucrose concentration. Gene 84, 181-185.
Sambrook, J., Fritsch, E.F. and Maniatus, T. (1989) Molecular cloning : A Laboratory Manual. 2nd edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
Schaffer, A.A., Aloni. B. and Fogelman. E. (1987) Sucrose metabolism and accumulation in developing fruit of cucumis. Phytochemistry 26, 1883-1887.
Schwarts, D. (1960) Electrophoretic and immunochemical studies with endosperm proteins of maize mutants. Genetics 45, 1419-1427.
Shaw, J.R., Ferl, R.J., Baier, J., St.Clair, D., Carson, C. and McCarty, L.C. (1994) Structural features of maize Sus1 gene and protein. Plant Physiol. 106, 1659-1665.
Short, J.M., Fernandez, J.M., Sorge, J.A. and Huse, W.D. (1988) λZAP: a bacteriophage λexpression vector with in vivo excision properties. Nucl. Acids Res. 16, 7583-7600.
Silvius, J.E. and Snyder, F.W. (1979) Comparative enzymatic studies of sucrose metabolism in tap roots and fibrous roots of Beta vulgaris L. Plant Physiol. 64, 1070-1073.
Sinha, A.K., Pathre, U.V. and Sane, P.V. (1998) Essential histidyl residues at the active site(s) of sucrose —phosphate synthase from Prosopis juliflora. Biochem. Biophys. Acta. 1388, 397-404.
Sonnewald, U., Hajirezaei, M.R., Kossmann, J., Heyer, A., Trethewey, R.N. and Willmitzer, L. (1997) Increased potato tuber size resulting from apoplastic expression of a yeast invertase. Nature Biotechnol. 15, 794-797.
Sturm, A. and Tang, G.Q. (1999) The sucrose-cleaving enzymes of plants are crucial for development, growth and carbon partitioning. Trends Plant Sci. 4, 401-407.
Sturm, A., Lienhard, S., Schatt, S. and Hardegger, M. (1999) Tissue-specific expression of two genes for sucrose synthase in carrot (Daucus carota L.). Plant Mol. Biol. 39, 349-360.
Su, J.C. (1965) Carbohydrate metabolism in the shoots of bamboo Leleba oldhami. I Preliminary survey of soluble saccharides and sucrose-degrading enzymes. Bot. Bull. Acad. Sinica, 6, 153-159.
Su, J.C., Wu, J.L and Yang, C.L. (1977) Purification and properties of sucrose synthase from maize kernels. Plant Physiol. 61, 389-393.
Sung, H.Y., Yuan, H.F. and Su, J.C. (1971) Carbohydrate metabolism in the shoots of bamboo Leleba oldhami. Ⅸ Some properties of the pentosan synthesizing system. J. Chin. Agric. Chem. Soc. Sp Iss, 62-73.
Sung, H.Y. and Su, J.C. (1977) Sucrose synthase isozymes of pea seedling - Purification and general properties. J. Chin. Biochem. Soc. 6, 22-37.
Tanase, K. and Yamaki, S. (2000) Purification and characterization of two sucrose synthase isoforms from Japanese pear fruit. Plant Cell Physiol. 41, 408-414.
Tang, G.Q. and Sturm, A. (1999) Antisense repression of sucrose synthase in carrot (Daucus carota L.) affects growth rather than sucrose partitioning. Plant Mol. Biol. 41, 465-479.
Towbin, H., Staehelin, T. and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Nalt. Acad. Sci. USA 76, 4350-4354.
Tupy, J. and Primot, L. (1982) Sucrose synthetase in the latex of Hevea Brasiliensis Muell. Arg. J. Exp. Bot. 33, 988-995.
Wang, A.Y., Yu, W.P., Juang, R.H., Huang, J.W., Sung, H.Y and Su, J.C. (1992) Presence of three rice sucrose synthase cDNA. Plant Physiol. 103, 1463-1464.
Wang, F.A., Sanz, A., Brenner, M.L. and Smith, A. (1993) Sucrose synthase, starch accumulation and tomato fruit sink strength. Plant Physiol. 101, 321-327.
Wang, F., Smith, A.G. and Brenner, M.L. (1994) Temporal and spatial expression pattern of sucrose synthase during tomato fruit development. Plant Physiol. 104, 535-540.
Weber, H., Buchner, P., Borisjuk, L. and Wobus, U. (1996) Sucrose metabolism during cotyledon development of Vicia faba L. is controlled by the concerted action of both sucrose-phosphate synthase and sucrose synthase : expression patterns, metabolic regulation and implications for seeds development. Plant J. 9, 841-850.
Weber, H., Borisjuk, L., Heim, U., Sauer, N. and Wobus, U. (1997) A role for sugar transporters during seed development: molecular characterization of a hexose and a sucrose carrier in fava bean seed . Plant Cell 7, 1835-1846.
Winter, H., Huber, J.L. and Huber, S.C. (1997) Membrane association of sucrose synthase: changes during the graviresponse and possible control by protein phosphorylation. FEBS Lett. 420, 151-155.
Wittich, P.E. and Vreugdenhil, D. (1998) Localization of sucrose synthase activity in developing maize kernels by in situ enzyme histochemistry. J. Exp. Bot. 324, 1163-1171.
Wolosiuk, R.A. and Pontis, H.G. (1971) Evidence of the existence of two forms of sucrose synthetase. FEBS Lett. 16, 237-240.
Yeh, D.B. and Su, J.C. (1972) Purification and characterization of sucrose synthetase from banana fruits. J. Chin. Biochem. Soc. 1, 37-51.
Yoshinaga, F., Tonouchi, N. and Watanabe, K. (1997) Research progress in production of bacterial cellulose by aeration and agitation culture and its application as a new industrial material. Biosci. Biotech. Biochem. 61, 219-224.
Yu, W.P., Wang, A.Y., Juang, R.H., Sung, H.Y. and Su, J.C. (1992) Isolation and sequence of rice sucrose synthase cDNA and genomic DNA. Plant Mol. Biol. 18, 139-142.
Zeng, Y., Wu, Y., Avigne, W.T. and Koch, K.E. (1998) Differential regulation of sugar-sensitive sucrose synthases by hypoxia indicate complementary transcriptional and post transcriptional response. Plant Physiol. 116, 1573-1583.
Zhang, X.Q. and Chollet, R. (1997) Seryl-phosphorylation of soybean nodule sucrose synthase (nodulin-100) by a Ca2+-dependent protein kinase. FEBS Lett. 410, 126-130.
Zhang, X.Q., Lund, A.A., Sarath, G., Cerny, R.L., Roberts, D.M. and Chollet, R. (1999) Soybean nodule sucrose synthase (nodulin-100): further analysis of its phosphorylation using recombinant and authentic root-nodule enzymes. Arch. Biochem. Biophys. 371, 70-82.
Zrenner, R., Salanoubat, M., Willmitzer, L. and Sonnewald, U. (1995) Evidence of the crucial role of sucrose synthase for sink strength using transgenic potato plants (Solanum tuberosum L.). Plant J. 7, 97-107.
王素秋 (1989) 豌豆蔗糖合成之研究,碩士論文,國立台灣大學農業化學研究所
王愛玉 (1989) 水稻蔗糖合成之分子生物學研究,博士論文,國立台灣大學農業化學研究所
宋賢一 (1973) 蔗糖合成酵素在植物醣類代謝之功用,博士論文,國立台灣大學農業化學研究所
李晏忠 (1997) 水稻蔗糖合成異構基因表現之研究,碩士論文,國立台灣大學農業化學研究所
岩素芬 (1992) 水稻蔗糖合成異構之純化與鑑定,博士論文,國立台灣大學農業化學研究所
高茂涵,王愛玉 (1999) 蔗糖、光照及溫度對水稻白化苗蔗糖合成異構含量之影響。中國農業化學會誌 37,207-214。
張久瑗 (1978) 綠竹筍之蔗糖合成酵素之純化及第四級結構之探討,碩士論文,國立台灣大學農業化學研究所
莊榮輝 (1985) 水稻蔗糖合成之研究-其純化、生物化學及免疫性質之比較,博士論文,國立台灣大學農業化學研究所
黃如瑋 (1994) 水稻蔗糖合成異構基因之選殖及影響表現生理條件的研究,博士論文,國立台灣大學農業化學研究所
黃瀅如 (2000) 綠竹筍蔗糖合成之純化與生化性質探討,碩士論文,國立台灣大學農業化學研究所
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