臺灣博碩士論文加值系統

近來，文獻指出膳食纖維會藉由腸系黏膜淋巴結調節小腸的免疫功能。為了研究膳食纖維對小腸黏膜淋巴組織─培耶氏斑的影響，實驗設計選擇三週大Wistar公鼠，分別以5% ( 一般餵食 ) 及10% 纖維素、10% 米糠和10% 小麥麩皮為膳食纖維來源，對照組為不含任何膳食纖維，餵食八週後犧牲。利用酵素結合分析法 ( ELISA ) 測血漿中的免疫球蛋白和小腸中分泌性免疫球蛋白A，免疫組織化學染色法觀察培耶氏斑中B、T細胞和IgA的分佈。結果為膳食纖維並不會影響老鼠的體重及攝食情形。免疫組織化學染色觀察膳食纖維餵食組B細胞的分佈是集中於發生中心，但對照組是零星分散於整個濾泡。T細胞大量表現濾泡間區位置，實驗組的圓頂區 ( dome area ) 亦有T細胞的分布，並為對照組多。IgA的分佈明顯集中於濾泡區 ( follicle ) 環圍，而對照組是散佈於濾泡。ELISA測得5%纖維素組中血清IgG和IgA以及 sIgA 均顯著高於對照組，此結果引申膳食纖維不但可能刺激腸道區域性免疫組織分化和免疫細胞的遷移，也可能影響全身性免疫系統。

Modulation of intestinal immune function related to mesenteric lymph node by dietary fiber had been studied recently. In order to investigate the physiological roles of dietary fiber on intestine epithelium mucosal lymphoid tissue-peyer's patches (PP), 3-week-old male Wistar rats were fed with 8-week cellulose (5 and 10g/100g diet), rice bran (10 g/100 g diet), and wheat bran (10g/ 100g diet) as well as without any dietary fiber supplement as a control group. Plasma samples and secretory IgA of intestinal mucus were drawn for quantity of immunological proteins by enzyme-linked immunosorbant assay (ELISA).The distribution of B and T lymphocytes as well as IgA-plasma cells of PP observed by immunohistochemistry. When Wistar rats were fed with the diets containing various dietary fiber for 8 weeks, there was no significant difference among the five groups, neither in the food intake nor weight gain. Frozen section of immunohistochemistry had showed that there were more B lymphocyte staining in the germinal center of rats fed with dietary fiber compared to control group that B lymphocyte was dispersedly distributed in PP. There were also more T lymphocyte staining in the interfollicle area and dome area of PP in the rats with fiber treatment compared with control group. As B lymphocyte, there was IgA-plasma cell distributed circlely in the follicle of rats fed with dietary fiber but control group showed no pattern at all. Compared to the control group, the plasma IgG and IgA as well as sIgA detected by ELISA were significantly higher in the group fed with 5% cellulose. This results imply that dietary fiber not only stimulated differention of local immunity and lymphocyte migration, and also modulated the immune system in the whole system.

表目錄
圖目錄
中文摘要……………………………………………………………..….I
英文摘要……………………………………………………………..….II
第一章前言………………………………………………………….…1
第二章文獻回顧…………………………………………………….…3
第一節 腸管上皮黏膜細胞區域性免疫組織………………………3
第二節 培耶氏斑的B細胞免疫調節反應…………………………7
第三節 IgA的分泌及功能………………………………………....10
第四節 膳食纖維和腸道環境之關係…………………...…………12
第五節 膳食纖維和腸道免疫之關係……………………………...14
第三章研究動機與目的………………………………………………19
第四章材料與方法……………………………………………………21
第五章結果……………………………………………………………29
第六章討論……………………………………………………………33
參考文獻………………………………………………………………..71
表目錄
表一：膳食纖維對老鼠攝食和成長的影響………………………….39
表二：不同種類的膳食纖維餵食八週後各組平均體重…………….40
表三：不同種類的膳食纖維餵食八週後平均各組小腸段培耶氏斑
數……………………………………………………………… 41
表四：不同種類的膳食纖維餵食八週後各組血清免疫球蛋白IgA
量……………………………………………………………..42
表五：不同種類的膳食纖維餵食八週後各組血清免疫球蛋白IgG
含量……………………………………………………………43
表六：不同種類的膳食纖維餵食八週後各組血清免疫球蛋白IgM
含量……………………………………………………………44
表七：不同種類的膳食纖維餵食八週後各組小腸中分泌性免疫球
蛋白SIgA含量………………………………………………..45
圖目錄
圖一：迴腸上皮黏膜細胞區域性免疫組織培耶氏斑的結構圖……..46
圖二：M細胞的構造…………………………………………………..47
圖三：發生中心的成熟…………………………………………………48
圖四：分泌性免疫球蛋白A的構造和形成…………………………..49
圖五：不同種類的膳食纖維餵食八週後各組平均體重………………50
圖六：不同種類的膳食纖維餵食八週後各組平均小腸培耶氏斑數
量…………………………………………………………………51
圖七：不同種類的膳食纖維餵食八週後各組血漿免疫球蛋白IgA…52
圖八：不同種類的膳食纖維餵食八週後各組血漿免疫球蛋白IgG…53
圖九：不同種類的膳食纖維餵食八週後各組血漿免疫球蛋白IgM…54
圖十：不同種類的膳食纖維餵食八週後各組小腸中分泌性免疫球
蛋白IgA………………………………………………………….55
圖十一：三至四週齡大Wistar公鼠餵食不含任何膳食纖維8週後
培耶氏斑冷凍切片免疫化學染色法─B細胞………………….56
圖十二：三至四週齡大Wistar公鼠餵食10% 纖維素8週後培耶
氏斑冷凍切片免疫化學染色法─B細胞……………………….57
圖十三：三至四週齡大Wistar公鼠餵食一般飼料 ( 5% 纖維素 )
8週後培耶氏斑冷凍切片免疫化學染色法─B細胞…………..58
圖十四：三至四週齡大Wistar公鼠餵食10% 米糠8週後培耶氏
斑冷凍切片免疫化學染色法─B細胞………………………….59
圖十五：三至四週齡大Wistar公鼠餵食10% 小麥麩皮8週後培
耶氏斑冷凍切片免疫化學染色法─B細胞…………………….60
圖十六：三至四週齡大Wistar公鼠餵食不含任何膳食纖維8週後
培耶氏斑冷凍切片免疫化學染色法─T細胞………………….61
圖十七：三至四週齡大Wistar公鼠餵食10% 纖維素8週後培耶
氏斑冷凍切片免疫化學染色法─T細胞……………………….62
圖十八：三至四週齡大Wistar公鼠餵食一般飼料 ( 5% 纖維素 )
8週後培耶氏斑冷凍切片免疫化學染色法─T細胞…………...63
圖十九：三至四週齡大Wistar公鼠餵食10% 米糠8週後培耶氏
斑冷凍切片免疫化學染色法─T細胞………………………….64
圖二十：三至四週齡大Wistar公鼠餵食10% 小麥麩皮8週後培
耶氏斑冷凍切片免疫化學染色法─T細胞…………………….65
圖二十一：三至四週齡大Wistar公鼠餵食不含任何膳食纖維8週
後培耶氏斑冷凍切片免疫化學染色法─IgA…………………..66
圖二十二：三至四週齡大Wistar公鼠餵食10% 纖維素8週後培
耶氏斑冷凍切片免疫化學染色法─IgA………………………..67
圖二十三：三至四週齡大Wistar公鼠餵食一般飼料 ( 5% 纖維素 )
8週後培耶氏斑冷凍切片免疫化學染色法─IgA……………...68
圖二十四：三至四週齡大Wistar公鼠餵食10% 米糠8週後培耶
氏斑冷凍切片免疫化學染色法─IgA…………………………..69
圖二十五：三至四週齡大Wistar公鼠餵食10% 小麥麩皮8週後
培耶氏斑冷凍切片免疫化學染色法─IgA……………………..70

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