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研究生:蘇倩雯
研究生(外文):Su Chien-Wen
論文名稱:金銀花之促進酪胺酸酶活性成分研究
論文名稱(外文):Studies on the Tyrosinase Activity Enhancing Constituents of Lonicera japonica Thunb.
指導教授:黃克峰黃克峰引用關係
指導教授(外文):Huang Keh-Feng
學位類別:碩士
校院名稱:靜宜大學
系所名稱:應用化學系
學門:自然科學學門
學類:化學學類
論文種類:學術論文
論文出版年:2001
畢業學年度:89
語文別:中文
論文頁數:52
中文關鍵詞:天然仿曬劑金銀花活性成分
外文關鍵詞:natural tanning agentsLonicera japonica Thunb.active constituent
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金銀花是中藥忍冬(Lonicera japonica Thunb.)的花蕾,文獻記載具有清熱解毒之藥理活性。
在我們持續致力於化妝品天然原料之開發研究中,最近發現金銀花之萃取物具有酪胺酸酶(tyrosinase)活性增強之效果。
本研究以tyrosinase活性導向與管柱層析得到其活性成分,並經由光譜分析確立其結構,其活性測試亦一併完成。
In our continuing search for natural source ingredient from Chinese medicinal herbs, was found that extracts of Lonicera japonica Thunb.
showed the significant tyrosinase enhancing activity.
In this study, the active constituent of Lonicera japonica Thunb. was isolated by the process of tyrosinase activity assay and column chromato-graphy. The structure was elucdiated by the spectroscopic methods and its activity was also achieved.
目錄
頁次
中文摘要
英文摘要
壹、 前言
貳、 金銀花
一、植物介紹
二、金銀花之成分研究
參、 活性測試總論
一、 皮膚之生理結構
1、 表皮(Epidermis)
2、 真皮(Dermis)
3、 皮下組織(Subcutis)
二、 黑色素(Melanin)之形成過程
1、 黑色素之定義
2、 黑色素之種類
(1) 真黑色素(Eumelanin)
(2) 褐黑色素(Pheomelanin)
3、 黑色素細胞(Melanocyte)
4、 黑色素生成(Melanogensis)之機轉
(1)黑色素之生化合成路徑
(2)黑色素細胞中之黑色素生成
5、 黑色素之功用
三、 酪胺酸酶(Tyrosinase)與黑色素之關係
1、 酪胺酸酶
2、 影響酪胺酸酶活性之因子
(1) 內分泌之影響
(A) 黑色素細胞刺激素(MSH)
(B) 褪黑激素(melatonin)
(2) 化學方面之影響
(A) 苯丙胺酸(Phenylalanine)
(B) 硫氫化物類(-SH-group)
(C) 金屬
(D) 還原劑
(3) 物理因素之影響
(A) 溫度
(B) pH值
(C) 陽光、紫外線
(D) 放射線
3、 酪胺酸酶活性與人類皮膚之關係
4、 酪胺酸酶影響皮膚顏色深淺之決定因子
(1) 皮膚中酪胺酸酶之活性
(2) 黑色素體之大小
(3) 酪胺酸酶合成與衰退的速率
(4) 黑色素體中合成黑色素之速率
(5) 黑色素體將黑色素運送至周圍角質細胞之效率
(6) 角質細胞中黑色素體之降解速率
四、 促進酪胺酸酶活性的物質
1、 直接操縱黑色素的形成
2、 解除酪胺酸酶的抑制
3、 增加酪胺酸酶的合成
4、 補骨脂的技術
5、 刺激酪胺酸酶的活性
6、 市面上常用來黑化皮膚的物質
五、 紫外線與皮膚的關係
肆、 實驗部分
一、 儀器
二、 試藥與溶劑
三、 金銀花之萃取與成分分離
四、 試藥配製
五、 測定tyrosinase activity之流程
伍、 結果與討論
一、 tyrosinase activity之測試結果
二、 金銀花50%酒精各分離之抽出率
三、金銀花50﹪Alcohol萃取物對tyrosinase activity之百分率
四、金銀花50﹪Alcohol萃取物之UV吸收
陸、 結論
柒、 參考文獻
捌、 圖表索引
柒、參考文獻
1. G. Prota, Med. Res. Rev., 8, 525. (1988).
2. T. J. Andreadis, US Paten 2 949 403, 16 August, (1960).
3. L''''Oreal, British Patent 954 920, 25 July, (1961).
4. P. R. Gordon, B. A. Gilchrest, J. Invest. Dermatol., 93, 700. (1989)
5. K. T. Lee, J. H. Kim, Proceeding of 3rd ASCS Conference, Taipei, Taiwan, p 34. (1997).
6. 華碧春、陳齊光: 忍冬藤和金銀花的本草研究。福建中醫學院學報, 6 (1) :27, 1996.
7. 顏焜熒,原色中藥飲片圖鑑,南天書局,臺北 p91, (1980).
8. 李永梅等:細毯毛忍冬花蕾化學成分研究。中國中藥雜誌,26 (1) , 2001.
9. T. Nakaoki, Yakugaku Zasshi, 69, 320. (1949).
10. 吳元流、方洪矩: 金銀花揮發油化學成分研究。化學學報, 38(6):573, 1980.
11. W. Chang and F. L. Hsu, Master thesis. Taipei Medical College, Taipei,
(1990).
12. W. C. Chang, F. L. Hsu, Prostaglandins Leukotrienes & Essential Fatty Acids, 45(4), 307. (1992).
13. J. T. Cheng, Y. Y. Lee, F. L. Hsu, W. Chang, C. S. Niu, Chin. Pharm. J., 46(6),575. (1994).
14. C. W. Chang, M. T. Lin, S. S. Lee, K. C. S. Chen Liu, F. L. Hsu, J. Y. Lin, Antiviral Research, 27, 367. (1995).
15. S. J. Lee, S. E. Joo, S. K. Ho, C. H. Wook, K. S. Sik, K. H. Pyo, Arch. Pharm. Research, 18(2), 133. (1995).
16. 高玉敏、王名洲、王建平: 金銀花化學成分的研究。中草藥, 26, 568, (1995).
17. 黃麗瑛、呂植偵、李繼彪: 中藥金銀花化學成分的研究。中草藥, 27, 645, (1996).
18. 婁紅祥等: 金銀花中水溶性化合物的分離與結構鑑定。中草藥, 27, 195, (1996).
19. W. S. Schlotzhauer, Volatile constituents from the flowers of Japanese Honeysuckle (Linicera japonica), J. Agric. Food. Chem., 44, 206-209. (1996).
20. M. T. Chul, P. J. Ok, C. K. Won, S. K. Ho, K. H. Pyo, K. S. Sik, C. H. Wook, C. K. Charn, Yakhak Hoeji, 43(1) 117 (1999).
21. R. Kakuda: Secoiridoid glycosides from the flower buds of Linicera japonica, Phytochemistry, 55, 879-881 (2000).
22. 周德成: 解剖生理學,昭人出版社,台北,p82-89. 1989.
23. 周燕輝: 皮膚病,正中書局,台北, 1-10. 1987.
24. 黃美勳: 中藥對酪胺酸酵素氧化作用之活性研究,中國醫藥學院碩士論文, 1996.
25. 黃效民: 黑色素之功能。食品工業月刊,四月號, p8-12. 1996.
26. J. M. Pawelek, and A. M. Korner. The biosynthesis of mammalian melanin. American Scientist. 70, 136-145 (1982).
27. G. Prota, Recent advances in the chemistry of melanogensis in mammals. J. Invest. Dermatol. 75, 122-127. (1980).
28. 劉承煌,皮膚病理學。中國醫藥科技出版社。北京, p74-98. 1991.
29. Lowell A, Golgsmith: Biochemistry and Physiology of the skin. Oxford University Press, Inc, (1983).
30. A., J. Sanchez-Ferrer, N. Rodriguez-Lopez and F. Garcia-Canovas. Tyrosinase: a comprehensive review of its mechanism. Biochim. Biophys. Acta. 1247: 1-11. (1995).
31. C. B. Keith, Lawrence M: Melanins: Hair dyes for the future. Cosmetics & Toiletries, 109: 59-64. (1994).
32. 楊濟華: 部分中草藥美白與防曬成份研究,靜宜大學應用化學研究所碩士論文. 1996.
33. L. A. Golgsmith, Biochemistry and Physiology of the Skin. Oxford University Press , Inc. (1983).
34. H. J.Niggli, Comparative studies on the correction between pyrimidine dimer formation and tyrosinase activity in Cloudman S-91 melanoma cell after ultraviolet-irradiation. Photochem. Photobiol. 52: 519-524. (1990).
35. A. B. Lerner, and T. B. Fitzpatrick. Biochemistry of melanin formation. Physiol. Rev. 30:91-126. (1950).
36. B. B. Fuller, J. B.Lunsford and D. S. Iman.α-Melanocyte - stimulating hormone regulation of tyrosinase in cloudman S-91 melanoma cell cultures . J. Biol. Chem. 262: 4024-4033. (1987).
37. M, K. Jimenez, W. L. Kameyama, Y. Maloy, Tomita and V. J. Hearing. Mammalian tyrosinase: Biosynthesis, processing and modulation by melanocyte-stmulating hormone. Proc. Natl. Acad. Sci. USA. 85: 3830-3834. (1988).
38. B. B. Fuller and D. H. Viskochil. The role of RNA and protein synthesis in mediating the action of MSH on mouse melanoma cells. Life Sci. 24: 2405-2416. (1979).
39. G. Wong, and J. Pawelek. Melanocyte-stimulating hormone promotes activation of pre-existing tyrosinase molecules in cloudman S-91 melanoma cells. Nature. 255: 644-646. (1975).
40. S. A. Burchill, R. Viren, B. B. Fuller and A. J. Thoday. Regulation of tyrosinase synthesis byα-melanocyte-stimulating hormone in hair Follicular melanocytes of the mouse. J. Endocr. 116: 17-23 (1988).
41. S. A. Burchill, A. J. Thody and S. Ito. Melanocyte-stimulating hormone, tyrosinase activtity and the regulation of eumelanogenesis and phaeomelanogenesis in the hair follicular melanocytes of the mouse. J. Endocr. 109: 15.21 (1986).
42. B. Weatherhead, and A. Logan. Interaction of α-melanocyte- stimulation hormone, melatonin, cyclic AMP in the control of melanogenesis in hair follicular melanocytes in vitro. J. Endocr. 90: 89-96 (1981).
43. P. L. Altmeyer, Stohr and H. Holzmann. Seasonal rhythm of the plasma level of α-melanocyte stimulating hormone. J. Invest. Dermatol. 86: 454-456 (1986).
44. J. R. Jara, F. Solano, J. C. Garcia-Borron, P. Aroca and J. A. Lozano. Regulation of mammalian melanogensis. The role of metal cations. Biochimica et Biophysica Acta.1035: 276-285 (1990).
45. D. C. Liebler, The role of metabolism in the antioxidant function of vitamin E. Critical Review in Toxicology. 23: 147-169 (1993).
46. K. T. Ram, C. D. Chintamani and R. Abburi. pH-dependent interconversion of two forms of tyrosinase in human skin. Biochem. J. 252:481-487 (1988).
47. Christine Romero, Edith Aberdam: Retinoic acid as modulator of UVB-induced melanocyte differentiation. Journal of Cell Science, 107: 1095-1130 (1994).
48. W. C. Quevedo, G. Szabo and J. Virks. Melanocyte populations in UV-irradiated human skin. J. Invest. Dermatol. 45: 295-298 (1965).
49. M. D. Mitsuru, B. S. Todd, D. D. S. Shoko, M. D. Everett and B. B. Fuller. The relationship between tyrosinase activity and skin color in human foreskins. J. Invest. Dermatol. 95: 9-15 (1990).
50. K. G. Iozumi, E. Hoganson, R. Pennella, M. A. Everett and B. B. Fuller. Role of tyrosinase as the determinant of pigmentation in cultured human melanocytes. J. Invest. Dermatol. 100: 806-811 (1993).
51. S. H. Pomerantz, and I. G. Ances. Tyrosinase activity in human skin. Influence of race and age in newborns. J Clin. Invest. 55:1127-1131 (1975).
52. K. W. Jimbow, C. Quevedo, T. B. Fitzpatrick and G. Szabo. Some aspects of melanin bibology. J. Invest. Dermatol. 67: 72-89 (1976).
53. W. C. Quevedo, The control of color in mammals. Am. Zool. 9: 531-540. (1969).
54. M. Seiji, K. Shimao, M. S. C. Birbck and T. B. Fitzpatrick. Subcellular localization of melanin biosynthesis. Ann NY Acad. Sci. 100:497-533 (1963).
55. M. Duggan, J. Wilmott and A. Znalden: Tyrosinase The Enzyme Behind the tan, Cosmetics and Toiletries Vol. 102, p 97-101, March (1987).
56. J. Lowe Nicholas and N. A Shaath: Sunscreen Marcel Dekker Inc. (1990).
57. Lisha Zhang, Takemi Yoshida and Yukio Kuroiwa: Stimulation of melanin synthesis of B16-F10 mouse melanoma cells by bufalin, Life Sciences, Vol. 51, NO. 1, p17-24 (1992).
58. A. K. Mathur, B. N.Gupta , R. Shanker, S. Narang , S. Singht and A. Singh: Dermal exposure of hexachlorophent to guinea pigs, Veterinary & Human Toxicology, 34 (1) : 4-6, Feb (1992).
59. W. Tur, A. G. Induchem, Tanning Accelerators: Increasing the effectiveness, Cosmetics & Toilertries Vol. 105. 79-85. Dec (1990).
60. F. Kevin Gallagber: Self-tanning the walnut way, SPF MARCH
p43.(1994).
61.M. A. pathak: Sunscreens-progress and perspectives on photorotectionof human skin against UVB and UVA radiation, The Journal of Dermatology, 23: 783-800. (1996).
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