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研究生:陳思樺
研究生(外文):SzuHuaChen
論文名稱:表現依鈣蛋白質分解酵素抗轉錄核醣核酸對其轉殖小鼠生長及體組成的影響
論文名稱(外文):Regulation of Growth Performance and Body Composition by Expression of m-calpain Antisense RNA in Transgenic Mice
指導教授:歐柏榮
指導教授(外文):BorRungOu
學位類別:碩士
校院名稱:東海大學
系所名稱:畜產學系
學門:農業科學學門
學類:畜牧學類
論文種類:學術論文
論文出版年:2001
畢業學年度:89
語文別:中文
論文頁數:99
中文關鍵詞:依鈣蛋白質分解酵素抗轉錄核醣核酸轉殖小鼠生長體組成
外文關鍵詞:calpainantisense RNAtransgenic micegrowth performancebody composition
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本研究的目的是分析表現依鈣蛋白質分解酵素抗轉錄核醣核酸對其轉殖小鼠生長性能及體組成的影響。
以 PCR及南方點墨法的分析得知,被轉殖帶有依鈣蛋白質分解酵素抗轉錄核醣核酸質體之外源性基因之小鼠經過數代的繁殖仍可偵測到此外源基因,表示此外源性基因穩定的存在。轉殖公小鼠、非轉殖公小鼠、轉殖母小鼠及非轉殖母小鼠的體重在2.5-3月齡才有明顯的不同,但是採食量並沒有明顯差異,這說明基因轉殖小鼠體重的增加並非是因為採食量增加之故,而可能是由於體內能量代謝改變所致。
小鼠體組成之分析,顯示轉殖公小鼠、非轉殖公小鼠、轉殖母小鼠及非轉殖母小鼠在水分及粗蛋白的含量並沒有差異,但是轉殖公小鼠之粗脂肪含量在2.5月齡時顯著較非轉殖公小鼠者為高;轉殖母小鼠之粗脂肪含量在 3月齡顯著較非轉殖母小鼠者為高。由上述結果可知轉殖公小鼠及轉殖母小鼠分別在 2.5 及 3 月齡時顯著比非轉殖小鼠來得重,可能是因為細胞降低蛋白質分解所累積的能量在體內以脂肪的型式堆積所致。

The objective of this study was to investigate the effects of m-calpain antisense RNA on growth performance and body composition in transgenic mice. M-calpain cDNA fragment was inserted into pMAMneo vector, then the transgene was transferred into mouse embryo by microinjection. Transgenic mice were identified by polymerase chain reaction (PCR) and Southern blot. The body weights of transgenic male mice at 2.5 month old were higher than those of non-transgenic male mice at the same age. In addition, the body weights of transgenic female mice at 3 month old were higher than those of non-transgenic female mice at the same age. The concentrations of m-calpain in various tissues were determined by Western blot. To understand the possible mechanism, the body composition, water content, crude fat content and crude protein content were examined as well . There was no difference in water content and crude protein content between transgenic and non-transgenic mice. However, The crude fat content in transgenic mice was higher than those in non-transgenic mice. This result implied that the energy saved from reduction of protein degradation contributed to the accumulation of adipose tissue in transgenic mice.

目錄
頁次
摘要……………………………………………………………..1
緒言……………………………………………………………..2
文獻檢討………………………………………………………..3
壹、骨骼肌蛋白質分解的控制機制………………………..3
貳、依鈣蛋白質分解酵素 (calpain) 之簡介……………….6
參、依鈣蛋白質分解酵素及其內源性抑制劑 calpastatin 在動物體內各個組織中之表現以及對動物生長之影響……………………………………………………....28
肆、抗轉錄核糖核酸 (antisense RNA) 之應用……………32
材料與方法…………………………………………………....36
結果…………………………………………………………....45
討論…………………………………………………………....64
結論…………………………………………………………....68
參考文獻……………………………………………………....69
英文摘要………………………………………………………97
小傳……………………………………………………………99
圖次
頁次
圖 1. 依鈣蛋白質分解酵素之結構…………………………13
圖 2. 依鈣蛋白質分解酵素之內源性抑制物 calpastatin 之結構………………………………………………………21
圖 3. 依鈣蛋白質分解酵素自我活化的模式圖…………….25
圖 4. 依鈣蛋白質分解酵素經分離活化的模式圖………….26
圖 5. 依鈣蛋白質分解酵素自我活化及分離的模式圖…….27
圖 6. 質體 pMAMA 之圖譜………………………………...48
圖 7. 利用 PCR 鑑定基因轉殖小鼠之數目………………..49
圖 8. 利用 Southern blot 鑑定基因轉殖小鼠之數目………50
圖 9. 轉殖公小鼠與非轉殖公小鼠體重之比較…………….51
圖 10. 公小鼠體組成中水分之百分比……………………....53
圖 11. 公小鼠體組成中粗蛋白之百分比…………………...54
圖 12. 公小鼠體組成中粗脂肪之百分比…………………...55
圖 13. 轉殖母小鼠與非轉殖母小鼠體重之比較…………...56
圖 14. 母小鼠體組成中水分之百分比……………………...58
圖 15. 母小鼠體組成中粗蛋白之百分比…………………...59
圖 16. 母小鼠體組成中粗脂肪之百分比…………………...60
圖 17. 西方點墨法分析小鼠組織中 m-calpain 表現……....61
圖 18. 影像分析儀將圖16 數據化之結果………………….62
圖 19. 29週齡轉殖小鼠及非轉殖小鼠之照片………………63 
表次
頁次
表 1. 離乳後非轉殖公小鼠及轉殖公小鼠的採食量……….52
表 2. 離乳後非轉殖母小鼠及轉殖母小鼠的採食量……….57

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