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研究生:姚季光
研究生(外文):Yao Jih-Guang
論文名稱:果蠅複眼發育中EYEGONE(EYG)蛋白的功能區之分析
論文名稱(外文):Dissection of the Functional Domains of the EYE GONE (EYG) protein in Drosophila melanogaster
指導教授:孫以瀚孫以瀚引用關係
指導教授(外文):Y. Henry Sun
學位類別:碩士
校院名稱:國立陽明大學
系所名稱:遺傳學研究所
學門:生命科學學門
學類:生物訊息學類
論文種類:學術論文
論文出版年:2001
畢業學年度:89
語文別:英文
論文頁數:63
中文關鍵詞:果蠅複眼
外文關鍵詞:Drosophila eye development
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EYE GONE (EYG) 蛋白為果蠅複眼發育的主控因子之一,且是 Pax 轉錄蛋白族中的一員。若eyg基因突變喪失功能,會導致複眼無法產生;若eyg基因異位表現,則引起異位複眼發生。此外,在我們實驗室之前的研究得知,EYG蛋白會抑制複眼抑制基因wg和omb表現。EYG蛋白具有兩個保留性高的DNA結合區: RED 區 (RD)及homeodomain區 (HD)。在我的論文研究中探討了EYG蛋白結構與功能的關係。針對EYG蛋白不同的功能區,我設計了一系列EYG的刪除構建(deletion construct),將它們以微注射送入果蠅胚胎,建立轉殖果蠅藉由GAL4/UAS 系統將它們誘發表現在特定的組織或細胞中,以利於在活體中分析EYG蛋白在發育過程中的功能。從我的結果得知:(1)對於恢復eyg喪失功能的突變所造成之無眼性狀及誘導異位複眼形成,RD及HD兩個功能區都是需存在,且不需C端的區域。(2)對於抑制 wg與 omb在眼盤兩側的表現,RD是必需但不充分。(3)對於抑制wg在眼盤背側的表現,HD是必需但不充分,但對於抑制omb在眼盤兩側的表現,及抑制wg在眼盤腹側的表現,HD卻不是必需的。因此RD與HD這兩個不同的DNA結合區在果蠅的複眼發育中扮演著不同的調控功能。

EYE GONE (EYG), a transcription factor of the Pax family, is a master regulator of Drosophila eye development. Its loss-of-function mutation abolished eye development. Ectopic eyg expression induces ectopic eyes formation, and suppresses the anti-eye genes, wg and omb. EYG has two conserved DNA-binding domains, the RED domain (RD) and the homeodomain (HD). In order to determine which domain(s) is required for EYG functions in eye development, a series of deletion constructs were designed and transformed into flies. The transgenes were induced to express in specific tissues using the GAL4/UAS system. The results showed that (1) the RD and HD are both required, and together they are sufficient, for the rescue of “no-eye” phenotype of eyg mutant and for the induction of ectopic eye, (2) the RD is required but not sufficient for the suppression of wg and omb transcription in eye disc and (3) the HD is required but not sufficient for the suppression of wg transcription at dorsal margin, and is not required for the transcription suppression of wg in the ventral margin and of omb in both dorsal and ventral margins of the eye disc. Thus, the two different DNA-binding domains play different functions during eye development.

中文摘要 -----------------------------------------------------1
Abstract ----------------------------------------------------2
Introduction ----------------------------------------------3-10
Results ----------------------------------------------11-23
Discussion --------------------------------------------24-28
Materials and Methods -----------------------------------29-36
References ---------------------------------------------37-40
Tables --------------------------------------------41-42
Figures -----------------------------------------------43-63

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