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研究生:朱朔嘉
研究生(外文):Sho-Chia Chu
論文名稱:自體螢光光譜與影像技術應用於癌組織診斷
論文名稱(外文):Autofluorescence Spectrum And Imaging Techniques for Cancer Tissue Diagnosis
指導教授:江惠華江惠華引用關係林禎國
指導教授(外文):Huihua Kenny ChiangJen-Kou Lin
學位類別:碩士
校院名稱:國立陽明大學
系所名稱:醫學工程研究所
學門:工程學門
學類:生醫工程學類
論文種類:學術論文
論文出版年:2001
畢業學年度:89
語文別:中文
論文頁數:53
中文關鍵詞:雷射誘發自體螢光影像結直腸癌診斷光譜
外文關鍵詞:autofluorescenceimagecolorectal cancerdiagnosisspectraEBCCD
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本研究是以自體螢光(Autofluorescence)光譜與影像研究正常組織與癌組織之間的差異,希望能以此為基礎進而診斷早期癌組織病變。量測系統現以敏感的ICCD(增強型電荷耦合元件)、Y型光纖、氮氣雷射、分光儀,建構成自體螢光光譜系統。用更敏感的EBCCD(電子轟擊性攝影機),前接Nikon鏡頭,並置換不同濾光鏡擷取影像,組成自體螢光影像量測系統。量測標本是離體結直腸組織為主;實驗結果顯示,光譜訊息中350~650nm平均自體螢光強度,正常組織、癌組織光譜明顯不同;藉此差異性可判讀組織是否癌化。影像方面,使用帶通濾光鏡擷取波段螢光影像和全光譜影像效果一樣,但顯示螢光影像比白光影像較能凸顯較佳的病灶範圍診斷。未來希望能將兩套系統結合,成為自體螢光光譜影像系統,作為早期癌組織診斷輔助儀器。
The goal of this research is to use autofluorescence spectra and image to differentiate cancer from normal tissues.In this research, we developed autofluorescence spectra measurement system and image measurement system. The former consists of a sensitive ICCD, a Nitrogen laser, a Y type fiber, a monochromate and a long-pass filter. The latter includes an even more sensitive EBCCD, a Nikon len , and a band-pass filter set. The measured sample is a piece of colorectal tissue from operation. The result of the spectra experiment shows characteristic spectrum, 350-650 nm waveform of the normal and cancerous tissues. These spectra characteristics enable the diagnosis of the tissues , cancerous or normal. In addition, the fluoresence image system is set to be developed for clinical colorectal tissue characterizations, including normal and cancer tissues. The band-passed spectral images are the same as the full spectral images , but the latter are better recognized than white light images. We will integrate the two systems together as an autofluorescence spectra image system for early cancer tissues diagnosis.
一、緒論
1.1引言 1
1.2研究動機與目的1
1.3前人研究2
1.4簡述各章節內容 4
二、研究背景
2.1結直腸之解剖位置與結構5
2.2結直腸癌8
2.3目前臨床診斷9
2.4醫療雷射12
2.5螢光產生機制13
2.6螢光光譜量測技術15
三、材料與方法
3.1儀器簡介17
3.2實驗架構26
3.3標本與實驗流程 28
四、實驗結果
4.1光譜量測 30
4.2影像擷取 37
五、討論
5.1病理因素42
5.2雷射光雜訊45
5.3螢光影像 46
5.4 ICCD與EBCCD影像比較46
六、結論與未來方向
參考文獻
[1] 中華民國行政院衛生署, ”中華民國八十六年癌症登記報告”, 2000.
[2] 中華民國行政院衛生署, ”中華民國八十八年死亡原因統計”, 2000.
[3] 王智昱, ”癌症組織之自體螢光診斷技術開發研究”, 博士論文, 1998.
[4] Chih-Yu Wang, Chin-Tin Chen, Chun-Pin Chiang, Shueng-Tsong Young, Song-
Nan Chow, and HuiHua Kenny Chiang, “A Probability-based multivariate statistical algorithm for autofluorescence spectroscopic identification of oral carcinogenesis,” Photochemistry and Photobiology, vol. 69, no. 4, pp. 471-7, 1999.
[5] C.Y. Wang, J. K. Lin, and Huihua Kenny Chiang, “Autofluorescence Spectroscopic Differentiation Between Normal and Cancerous Colorectal Tissues Using Two-Peak Ratio Algorithm,” J. Formos. Med. Assoc., vol. 98, no. 12, pp. 837-843, 1999.
[6] Chih-Yu Wang, Huihua Kenny Chiang, Chin-Tin Chen, Chun-Pin Chiang, Ying-Shiung Kuo, Song-Nan Chow, ”Diagnosis of oral cancer by light-induced autofluorescence spectroscopy using double excitation wavelengths,” European Journal of cancer, vol. 35, No. 2, pp. 144-50, 1999
[7] 孫家偉, ”大腸癌症組織自體螢光分期診斷技術開發研究”,碩士論文, 1999.
[8] 吳詩斌, “雷射誘發自體螢光影像技術應用於結直腸癌症與瘜肉診斷之開發研
究,碩士論文, 民89.
[9] T. J. Romer, M. Fitzmaurice, R. M. Corthren, R. Richards-Kortum, R. Petras,M.V. Sivak, Jr., J. R. Kramer Jr., “Laser-induced fluorescence microscopy of normal colon and dysplasia in colonic adenomas: implication for spectroscopic diagnosis,” Am. Coll. of astroenterology, vol. 90, No.1, pp.81-7, 1995.
[10] V. R. Kolli, H. E. Savage, T. J. Yao, S. P. Schantz, “Native cellular fluorescence of neoplastic upper aerodigestive mucosa. Photodynamic therapy in Otolaryngology─ Head and neck surgery,” Arch otolaryngel head neck surg., vol. 121, pp.1287-92, 1995.
[11] R. R. Alfano, G. C. Tang, A. Pradhan, “Fluorescence spectra from cancerous and
normal human breast and lung tissues,” IEEE J. Quant. Electr. QE-23, pp. 1806-
11, 1987.
[12]V. D. Tuan, “In vivo cancer diagnosis of the esophagus using differential normalized fluorescence indices,” Lasers in Surgery and Medicine, vol. 16, pp. 41-7, 1995.
[13]M. Panjehpour, “Spectroscopic diagnosis of esophageal cancer: new classification model, improved measurement system,” Gastrointestinal Endoscopy, vol. 41, no.6 , pp. 577-81, 1995.
[14]A. Mahadevan, M. F. Mitchell, E. Silva, S. Thomsen, R. R. Richard-Kortum, “A study of the fluorescence properties of normal and neoplastic human cervical tissue,” Lasers in Surgery and Medicine, vol. 13, pp. 647-55, 1993.
[15]G. Bottiroli, A. C. Corce, D. Locatelli, R. Marchesini, E. Pignoli, C. Cuzzoni, S. D. Palma, M. Dalfante, P. Spinelli, “Natural fluorescence of normal and neoplastic human colon: A comprehensive‘ex vivo’ study,” Lasers in Surgery and Medicine, vol. 16, pp.48-60, 1995.
[16]F. Koenig, F. J. Mcgovern,A. F. Althausen,T. F. Deutsch,and K.T.Schomacker.”
Laser induced autofluorescence diagnosis of bladder cancer,”The journal of urology,Vol.156,1597-1601,November 1996
[17]癌症研究組, ”大腸直腸癌診斷與治療之共識”, 衛生研究院, 1999.
[18] “大腸直腸癌”, 癌症諮詢中心癌症專欄.(http://www.uhealthy.com)
[19] 林楨國,「衛教常識:大腸直腸癌」, 台北榮民總醫院大腸直腸外科.
(http://www.vghtpe.gov.tw/~crs/Diease/crsnur/colcancer2.htm)
[20] 趙醫師, ”痔瘡大腸直腸醫學目錄”, 三軍總醫院.
(http://www.toplulu.com/med2.html)
[21] 莊壽洺 饒宇東, “基本病理學”,南山堂出版社, 1990.
[22] 胡明一等編譯, “人體解剖學”, 藝軒出版社, 1994.
[23] 光電協會, ”1999年我國雷射及應用產業概況(上)”, 光訊, 第83期, 2000.
[24] 光電協會, ”1999年我國雷射及應用產業概況(下)”, 光訊, 第84期, 2000.
[25]T. D. Wang. J. V. Dam, J. M. Crawford, E. A. Preisinger, Y. Wang, M. S. Feld, “Fluorescence endoscopic imaging of human colonic adenomas,” Gastroenterology, vol. 111, pp. 1182-91, 1996.
[26]T. D. Wang, J. M. Crawford, M. S. Feld, Y. Wang, I. Itzkan, J. V. Dam, “In vivo identification od colonic dysplasia using fluorescence endoscopic imaging,” Gastrointerestinal endoscopy, vol. 49 pp. 447-55,1999.
[27]M. L. Harries, S. Lam, C. MacAulay, J. Qu, B. Palcic, “Diagnostic imaging of the Laryngol. Oto., vol. 109, no. 2, pp. 108-10, 1995 Feb.
[28]M. Zargi, L. Smid, I. Fajdiga, B. Bubnic, J. Lenaric, P. Oblak, ‘Detection and localization of early laryngeal cancer with laser-induced fluorescence: preliminary report,” Eur. Arch. Otorhinolaryngol. Suppl., 1: S113-6, 1997.
[29]M. Zargi, L. Smid, I. Fajdiga, B. Bubnic, J. Lenaric, P. Oblak, “Laser induced fluorescence in diagnostics of laryngeal cancer,” Acta. Otolaryngol. Suppl. (Stockh)., vol. 527, pp. 125-7, 1997.
[30]J. Hung, S. Lam, J. C. LeRiche, B. Palcic, “Autofluorescence of normal and malignant bronchial tissue,” Lasers. Surg. Med., vol. 11, no. 2, pp. 99-105, 1991.
[31]A. E. Profio, D. R. Doiron, E. G. King, “Laser fluorescence bronchoscope for localization of occult lung tumors,” Med. Phys., vol. 6, no. 6, pp. 523-5, 1979.
[32]B. W. Chwirot, S. Chwirot, W. Jedrzejczyk, M. Jackowski, A. M. Raczynska, J. Winczakiewicz, J. Dobber, “Ultraviolet laser-induced fluorescence of human stomach tissues: detection of cancer tissues by imageing techniques,” Laser Surg. Med., vol: 21, no. 2, pp. 149-58, 1997
[33]伍秀菁等, ”儀器總覽-光學量測儀器單光儀”, 行政院國科會精儀中心, p5-8,1998.
[34]G. Bourg-Heckly,J. Blais,J.-J Padilla,O. Bourdon,J. Etienne,F.Guillemin,L.Lafay.
Endoscopic Ultraviolet-Induced Autofluorescence Spectroscopy of the Esophagus:
Tissue Characterization and Potential for Early Cancer Diagnosis. Endoscopy 2000;32(10):756-765
[35].M.Sackmann. Fluorescence Diagnosis in GI Endoscopy. Endoscopy 2000;332(12):
977-985
[36] Abraham Katzir. Lasers and Optical Fibers in Medicine. Academic Press,
Inc.1993,pp 76.
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