本研究是以自體螢光（Autofluorescence）光譜與影像研究正常組織與癌組織之間的差異，希望能以此為基礎進而診斷早期癌組織病變。量測系統現以敏感的ICCD（增強型電荷耦合元件）、Y型光纖、氮氣雷射、分光儀，建構成自體螢光光譜系統。用更敏感的EBCCD（電子轟擊性攝影機），前接Nikon鏡頭，並置換不同濾光鏡擷取影像，組成自體螢光影像量測系統。量測標本是離體結直腸組織為主；實驗結果顯示，光譜訊息中350~650nm平均自體螢光強度，正常組織、癌組織光譜明顯不同；藉此差異性可判讀組織是否癌化。影像方面，使用帶通濾光鏡擷取波段螢光影像和全光譜影像效果一樣，但顯示螢光影像比白光影像較能凸顯較佳的病灶範圍診斷。未來希望能將兩套系統結合，成為自體螢光光譜影像系統，作為早期癌組織診斷輔助儀器。

The goal of this research is to use autofluorescence spectra and image to differentiate cancer from normal tissues.In this research, we developed autofluorescence spectra measurement system and image measurement system. The former consists of a sensitive ICCD, a Nitrogen laser, a Y type fiber, a monochromate and a long-pass filter. The latter includes an even more sensitive EBCCD, a Nikon len , and a band-pass filter set. The measured sample is a piece of colorectal tissue from operation. The result of the spectra experiment shows characteristic spectrum, 350-650 nm waveform of the normal and cancerous tissues. These spectra characteristics enable the diagnosis of the tissues , cancerous or normal. In addition, the fluoresence image system is set to be developed for clinical colorectal tissue characterizations, including normal and cancer tissues. The band-passed spectral images are the same as the full spectral images , but the latter are better recognized than white light images. We will integrate the two systems together as an autofluorescence spectra image system for early cancer tissues diagnosis.

一、緒論
1.1引言 1
1.2研究動機與目的1
1.3前人研究2
1.4簡述各章節內容 4
二、研究背景
2.1結直腸之解剖位置與結構5
2.2結直腸癌8
2.3目前臨床診斷9
2.4醫療雷射12
2.5螢光產生機制13
2.6螢光光譜量測技術15
三、材料與方法
3.1儀器簡介17
3.2實驗架構26
3.3標本與實驗流程 28
四、實驗結果
4.1光譜量測 30
4.2影像擷取 37
五、討論
5.1病理因素42
5.2雷射光雜訊45
5.3螢光影像 46
5.4 ICCD與EBCCD影像比較46
六、結論與未來方向
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