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研究生:葉怡真
論文名稱:樟芝對血管內皮細胞之影響及保心血管疾病之機制探討
論文名稱(外文):The Antioxidation Effects of Antradria camphorata on Culture Human unbical
指導教授:楊新玲楊新玲引用關係許游章
學位類別:碩士
校院名稱:中國醫藥學院
系所名稱:營養研究所
學門:醫藥衛生學門
學類:營養學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:121
中文關鍵詞:樟芝醱酵液樟芝菌絲體動脈硬化共軛雙烯鍵丙二醛細胞凋亡電位改變低密度脂蛋白
外文關鍵詞:Fermented culture broth of A. camphorataAqueous extract of mycelia of A. camphoraatAtherosclerosisConjugate dieneMDAApoptosisElectrophoretic mobilityLow density lipoproteinLDL
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中 文 總 摘 要
樟芝 (子實體) 是台灣稀少特有的菇類,相傳具有解毒、抗癌及抗氧化等功效。本研究的目是有二:第一、探討樟芝菌絲體的醱酵液(FCBA)或樟芝菌絲體的萃取液(AEMA)是否具保護血管內皮細胞受自由基傷害及提升抗氧化能力。研究顯示,單獨低濃度 (<100μl/ml) 的FCBA或AEMA會促進內皮細胞生長;然而,高濃度 (>200μl/ml) 的FCBA或AEMA內皮細胞會造成毒性。內皮細胞加入FCBA 或 AEMA會抑制自由基誘發劑-AAPH (15 mM)對內皮細胞的氧化傷害;包括,存活率(Cell viability)明顯上昇,PGI2 生成減少,細胞內SOD活性增加。此外,加入FCBA 或 AEMA亦會保護AAPH誘發內皮細胞凋亡 (Apoptosis);包括,TUNEL實驗細胞凋亡的現象明顯減少,且會抑制Cytochrome C 、caspase-3、bax蛋白的表現及PARP的裂解減少,而增加bcl-2、catalase 、SOD 蛋白的表現。第二、研究樟芝保護低密度脂蛋白(Low density lipoprotein,LDL)氧化作用。研究發現,LDL加入FCBA 或 AEMA會抑制自由基誘發劑-銅離子 (CuSO4) 或AAPH 對LDL的氧化傷害;包括,延長共軛雙烯 (Conjugate diene) 的延遲作用時間(Lag phase),降低脂質過氧化物丙二醛(MDA)形成,延緩脂質過氧化物(Lipid peroxide)產生,抑制脂蛋白B(Apo B)的裂解,保護膽固醇不被氧化,及抑制LDL的電位改變 (Electrophoretic mobility)。總結,樟芝(FCBA或AEMA)可以保護血管內皮細胞自由基誘發的氧化傷害及抑制氧化型LDL的形成,進而降低動脈硬化的發生。
英 文 總 摘 要
Antrodia camphorata (here we mean its fruit body), a rare mushroom in Taiwan , traditionally is used as an antidote, anticancer and antioxidant drug. The first purpose of this study was to evaluate the ability of Fermented culture broth of A. camphorata (FCBA) 、aqueous extract of mycelia of A. camphoraat (AEMA) to protect human umbilical vein endothelia cell(HUVEC) from free radical-induced oxidative damge in vitro and investigate its antioxidant role. Results show that exposure of HUVEC to low concentration (<100 μl/ml) of FCBA or AEMA increases endothelial cells growth; however as exposure to high concentration (>200 μl/ml) of those drugs causes cellular toxicity. Oxidative stress and apoptosis were induced by (AAPH). HUVEC preincubated with FCBA or AEMA prior to the AAPH exposure resulted in a dose-dependent increase in resistnace to oxidative stress; results had showed that an increase in cell viability and intracelluar SOD activity, while a decrease in PGI2 production as the cells incubated with AAPH were pretreated with increasing concentrations of FCBA and AEMA; Also, preincubated with FCBA or AEMA protects HUVECs from AAPH-induced apoptosis; results had showed a decrease of apoptotic cells detected by TUNEL assay, an inhibition of cytochrome c release, bax production, caspase-3 and PARP degradation, while a increase in bcl-2, catalase and SOD expression. The second purpose of this study was to evaluate the ability of FCBA and AEMA to protect low density lipoprotein (LDL) from free radical (CuSO4)-induced oxidative damge in vitro. Pretreated with FCBA or AEMA protects LDL from CuSO4-induced oxidative damge in a dose- and time-dependent manner; results had showed an enlongation lag phase of conjuage diene, delayed lipid peroxide production, a decreas of apo B synhtesis, protection of cholesterol from oxidation, and inhibit the electrophoretic mobility of LDL as LDL incubated with AAPH were pretreated with increasing concentrations of FCBA and AEMA. In conclusion, both FCBA and AMEA protect endothelial cells from free-radical induced-oxidative stress and apoptosis and reduce ox-LDL production, further reducing the risk of atherosclerosis.
目 錄
中文總摘要………………………………………………………………………..1 英文總摘要………………………………………………………………………2
第一章……………………………………………………………………………3
壹、 摘 要…………………………………………………………………4
貳、 前 言 6
參、 文獻整理……………………………………………………………..6
肆、 實驗品器材…………………………………………………………23
伍、 實驗方法…………………………………………………………….26
陸、 實驗結果…………………………………………………………….41
柒、 討 論 53
第二章………………………………………………………………………….55
壹、 摘 要…………………………………………………………………56
貳、 前 言、文獻整理…………………………………………………..58
參、 實驗品器材…………………………………………………………68
肆、 實驗方法……………………………………………………………70
伍、 實驗結果……………………………………………………………80
陸、 討 論 85
總 結………………………………………………………………………….87
圖 表…………………………………………………………………………88
參考文獻…………………………………………………………………
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