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研究生:黃勝敏
研究生(外文):Shen-Min Huang
論文名稱:人類肝癌訊息傳遞蛋白之變化
論文名稱(外文):The Changes of Signaling Transduction Protein
指導教授:蕭 松 瑞
指導教授(外文):Song-Jui Shiow
學位類別:碩士
校院名稱:中山醫學大學
系所名稱:生物化學研究所
學門:生命科學學門
學類:生物化學學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:99
中文關鍵詞:肝臟腫瘤西方墨點法B型肝炎病毒PI-3-K相關蛋白
外文關鍵詞:HCC:Hepatocellular CarcinomaWesternblotHBV:Hepatitis B virusPI-3-K
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survival 和pro-apoptotic、anti-apoptotic蛋白的表現是否平衡與腫瘤的形成和擴散有密切的關係。本研究目的在探討PI-3-K相關蛋白、細胞凋亡相關蛋白及Bcl-2相關蛋白在人類Hepatocellular Carcinoma(HCC)的表現情形。本研究我們收集30個人類HCC 患者的肝臟腫瘤組織及週邊正常組織,其中包含14 個B型肝炎病毒相關的HCC患者及16 個非B型肝炎病毒相關之HCC患者肝臟組織,利用西方墨點法(Westernblot)來觀察PI-3-K相關蛋白:PI-3-K、Akt-p、Cdc42 和細胞凋亡相關蛋白:IAP、Caspase 3 、NF-κB及Bcl-2相關家族成員:Bcl-2、 Bad-p、 Mcl-1、Bax等蛋白的表現,結果顯示B型肝炎病毒陽性相關的HCC患者其PI-3-K、Cdc42、Bcl-2、Bad-p、Bax、NF-κB等蛋白過度表現其比率分別為7/14位(50.0%)、6/14位(42.9%)、7/14位(50.0%)、7/14位(50.0%)、7/14位(50.0%)、11/14位(78.6%)而Mcl 1蛋白則有8/14位(57.1%)表現是減少的,在非B型肝炎病毒感染之HCC患者其PI-3-K、Cdc42、Bcl-2、Bad-p、Bax 等蛋白表現是減少的其比率分別為7/16位(43.8%)、8/16位(50.0%)、8/16位(50.0%)、9/16位(56.3%)、8/16位(50.0%)而NF-κB蛋白只有5/16位(31.3%)是過度表現,另外IAP、Caspase 3、Akt-p等蛋白表現則無明顯的差異。本研究顯示B型肝炎病毒陽性相關的HCC患者其PI-3-K
、Cdc42、Bcl-2、Bad-p、Bax、NF-κB等蛋白是過度表現,對照於在非B型肝炎病毒感染之HCC患者蛋白表現,顯然二者造成人類 HCC的路徑是不相同的。期望此研究結果對於人類 HCC的預防或治療有所助益。

The expression of survival, pro-apoptotic, and anti-apoptotic protein is related with the tumor growth and progression. The purpose of this study is to investigate the expression of PI-3-K related protein, apoptotic related protein, and Bcl-2 related protein in human hepatocellular carcinoma. The tumor and surrounding normal liver tissue of 30 HCC patients were included. The 14 patients were HBV associated HCC, the other 16 patients were non-HBV associated HCC. We observed the expression of the PI-3-K related protein (PI-3-K, Akt-p, and Cdc42), apoptotic related protein (IAP, Caspase 3 and NF-κB), and Bcl-2 family related protein (Bcl-2, Bad-p, Mcl-1, and Bax) using the Western blot. Our study showed that the over-expression rate of PI-3-K, Cdc 42, Bcl-2, Bad-p, Bax, and NF-κB in HBV associated HCC patients are 50.0%, 42.9%, 50.0%, 50.0%, 50.0%, and 78.6%. There was 57.1% of suppression rate in Mcl-1 protein. In non-HBV associated HCC, the expression in 43.8% of PI-3-K, 50.0% of Cdc 42, 50.0% of Bcl-2, 56.3% of Bad-p, and 50.0% of Bax were decreased. There was 31.3% overexpression rate in NF-κB. The expression of IAP, Caspase 3, and Akt-p were non-significant. We concluded that the PI-3-K, Cdc 42, Bcl-2, Bad-p, Bax, and NF-κB are overexpressed in HBV associated HCC, but not in non- HBV associated HCC. The carcinogenesis pathway of HCC formation in HBV associated and non-HBV associated should be different.

目 錄
人類肝癌訊息傳遞蛋白之變化…………………………………1
縮寫表……………………………………………………………2
中文摘要…………………………………………………………3
英文摘要…………………………………………………………5
壹.緒論………………………………………………………….6
貳.研究目的…………………………………………………….26
參.材料與方法………………………………………………….29
肆.結果………………………………………………………….37
伍.討論………………………………………………………….50
陸.圖與表……………………………………………………….56
柒.參考文獻…………………………………………………….75

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