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研究生:許競爵
研究生(外文):Ching-Chueh Hsu
論文名稱:I.假孕大鼠子宮蛻膜形成過程中蛋白激酵素C異構體mRNA之表現.II.中草藥萃取物抑制肝臟纖維化功效之評估.
論文名稱(外文):I. The mRNA expressions of PKC isoforms during decidulization at pseudopregnant rats. II. Estimation of the inhibitory effect of Chinese herb extract on liver fibrosis.
指導教授:劉哲育劉哲育引用關係
指導教授(外文):Jer-Yuh Liu
學位類別:碩士
校院名稱:中山醫學大學
系所名稱:生物化學研究所
學門:生命科學學門
學類:生物化學學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:110
中文關鍵詞:蛻膜形成作用蛋白激酵素 C肝臟草藥纖維化肝星狀細胞
外文關鍵詞:decidualizationPKCliverherbfibrosishepatic stellate cells
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第一部分.
我們過去的研究中發現,在蛻膜形成(decidualization)的過程中,不論是在真懷孕或假懷孕大鼠中,蛋白激酵素C(PKC)異構體都參與其蛻膜的形成,因此我們猜測PKC可能參與調節蛻膜細胞(decidual cell)的增殖過程。本實驗藉由RT-PCR的技術來分析假孕大鼠蛻膜形成過程中,PKC異構物及其下游相關蛋白以及細胞增殖指標(cdc2,cyclin D1)之mRNA表現,再輔以DNA定序分析確定RT-PCR產物之專一性。結果發現PKCα mRNA表現量在假孕大鼠蛻膜形成過程第二天到第九天中有顯著的增加,而同時期中PKC相關轉錄因子c-jun,c-fos亦有明顯的增加,這些明顯的改變意味著在蛻膜組織中PKCα的活化。此外也發現在假孕大鼠蛻膜形成過程中PKCδ及PKCλ mRNA表現量增加,而PKCλ mRNA表現增加的同時細胞增殖指標(cdc2及cyclin D1)mRNA的表現量亦增加。因此由以上的實驗結果證實了PKC異構物參與子宮蛻膜的形成過程。
第二部分.
肝炎(Hepatitis)在台灣地區素有國病之稱。根據流行病調查發現肝炎與肝纖維化和肝硬化有相關性。肝纖維化是一個動態的過程,在初期是為可逆的階段。因此,目前許多研究室仍致力於尋找阻延肝纖維化之藥物,其中包含中草藥。另外,根據研究指出肝星狀細胞(hepatic stellate cells,HSCs)過度活化增殖是導致肝纖維化/肝硬化的主要細胞之一。本實驗旨在建立一「快速細胞篩選模式」來篩檢中醫理論中具抗肝纖維化中草藥之功效。實驗選用36種中草藥,以傳統水萃取法萃取後,利用活化與未活化態肝星狀細胞偵測其半數致死劑量(LD50)。結果顯示上述36種中草藥中,確有抗纖維化功效者為31種(86.1 %),進一步分析後發現其中22種(61.1 %)中草藥其對於未活化態肝星狀細胞較不具傷害性,顯示利用肝星狀細胞篩選模式對於抗肝纖維化中草藥之篩選可能較優於傳統之動物模式。

Part I.
Our previous study have demonstrated that the protein levels of PKC isoforms were altered in the proliferation stages of decidualization at pseudopregnant and pregnant rats. In this study, we measured the mRNA expressions of the PKC isoforms and their down-stream related genes by using RT-PCR. The product of RT-PCR was also checked by DNA sequence analysis. The results showed that the levels of PKCα mRNA was significantly increased from day 2 to day 9 in the decidualization. The mRNA expression of the transcription factors (c-jun and c-fos) were also significantly increased during the same times. These specified that the alterations in the PKCα may signify their activation in decidual tissues. In addition, the mRNA levels of the other PKC isoforms (PKCλ and PKCδ were also significantly increased during the decidualization. The increase in the PKCλ mRNA was paralleled with the increase in the mRNA levels of the proliferation markers (cdc2 and cyclin D1). Thus, These data confirmed that the expression of PKC isoforms may be involved in the development of the decidualization.
Part II.
Hepatitis is one of the most common diseases in Taiwan. Patients with hepatitis may lead to liver fibrosis and cirrhosis. Liver fibrogenesis, which is a dynamic, complex, and progressive process, may be reversible at initial stages. Recently, many labs are extremely focus on discovering new drugs from Chinese herb for anti-fibrosis of liver. According to previous research, to activate hepatic stellate cells have been clearly identified as the major cause of liver fibrogenesis. The present studies aim to set up a fast selective cell model to estimate the anti-fibrotic effects by Chinese herbs. LD50 was determined by the actived/inactived HSCs when treated with water extracts of 36 kinds of Chinese herb. Our results showed that totally 31 kinds of Chinese herbs (86.1%) were detected with the antifibrotic effects. Within these 31 herbs, there are 22 kinds (61.1%) have less damage on inactived HSCs compared with actived. Taken together, our results indicated that the HSCs model may be more suitable to estimate the anti-fibrotic effects of Chinese herbs than animal models.

第一部分:假孕大鼠子宮蛻膜形成過程中蛋白激酵素C異構體mRNA之表現
壹、中文摘要
貳、英文摘要
參、縮寫表
肆、前言
伍、實驗動機
陸、實驗方法與材料
柒、實驗結果
捌、討論
玖、參考文獻
拾、圖表
附錄
第二部分:中草藥萃取物抑制肝臟發炎功效之評估
壹、中文摘要
貳、英文摘要
參、縮寫表
肆、前言
伍、實驗動機
陸、實驗方法與材料
柒、實驗結果
捌、討論
玖、參考文獻
拾、圖表
附錄

Part I.
1.宋永魁、李茂盛、楊友仕、曾啟瑞、張明揚(1998)不孕症及生殖內分泌學,合記圖書出版社,p127-128
2.Mosher WD, Pratt W.F.(1991) Fecundity and infertility in the United States: incidence and trends. Fertil. Steril., 56:192
3.郭宗正編譯(1998)彩色圖解不孕症治療,台灣長年出版社,p7
4.郭宗正編譯(1998)彩色圖解不孕症治療,台灣長年出版社,p9-28
5.宋永魁、李茂盛、楊友仕、曾啟瑞、張明揚(1998)不孕症及生殖內分泌學,合記圖書出版社,p315-326
6.游士弘、金玉堂、簡利婷、溫慧勤和王鈴琪(1997)人工生殖之醫學與法律(上),當代醫學雜誌,24(7):89-94
7.戴步雲編譯(2001)Moore人體胚胎學,合記出版社,p25-31
8.戴步雲編譯(2001)Moore人體胚胎學,合記出版社,p131
9.Tang, B., Guller, S. and Gurpide, E.(1994) Mechanism of human endometrial stromal cells decidualization [Review]. Ann. N. Y. Acad. Sci., 734:19-25
10.Pijnenborg, R., Dixon, G., Robertson, W.B. and Brosens, (1980) rophoblastic invasion of human decidua from 8 to 18 weeks of pregnancy. Placenta, 1:3-19
11.Golander, A., Zakuth, V., Shechter, Y. and Spirer, Z.(1981) Suppresion of lymphocyte reactivity in vitro by a soluble factor secretion by explants of human decidua. Eur. J. Immunol., 11:849-851.
12.潘人榮編譯(2001)人體胚胎學,藝軒出版社,p107-109
13.Daly, D. H., Maslar, I. A. and Riddick, D. H.(1982)Prolactin production during in vitro decidualization of proliferative endometrium. Am. J. Obster. Gynecol., 145:672-678
14.Tang, B., Guller, S. and Gurpide, E.(1994) Mechanism of human endometrial stromal cells decidualization [Review]. Ann. N. Y. Acad. Sci., 734:19-25
15.Tang, B., Gurpide, E.(1993)Direct effect of gonadotropins on decidualization of human endometrial stromal cells. J. Steroid. Biochem. Mol. Biol., 47:115-121
16.Dey, SK., Villanueva, C., Chien, SM., Crist, RD.(1978) The role of histamine in implantation in the rabbit. J. Report Feril., 53:23-26
17.Takai, Y., Kishimoto, A., Kikkawa, U., Mori, T. and Nishizuka, Y. (1979) Unsaturated diclyglycerol as a possible messenger for the activation of Calcium-activated, phospholipid-dependent protein kinase system. Biochem. Biophys. Res. Commun., 91:1218-1224
18.Takai, Y., Kishimoto, A., Inoue, M. and Nishizuka, Y. (1977) Studies
on a cyclic nucleotic-independent protein kinase and its proenzyme in
mammalian tissue. J. Biol. Chem., 252:7603-7609.
19.Takai, Y., Kishimoto, A., Inoue, M. and Nishizuka, Y., Kawahara, Y., Mori, T. and Nishizuka, Y.(1979)Calcium-dependent activation of a ultifunctional protein kinase by membrane phospholipid. J. Biol. Chem. ,254:3692-3695.
20.Takai, Y., Kishimoto, A., Kikkawa, U., Mori, T. and Nishizuka, Y.(1979)Unsaturated diacylglycerol as a possible messenger for the activation of Calcium-activated, phospholipid-dependent protein kinase system. Biochem. Biophys. Res. Commun., 91:1218-1224.
21.Castagna, M., Takai, Y., Kaibuchi, K., Sano, K., Kikkawa, U. and Nishizuka, Y.(1982)Direct activation of calcium-activated phospholipid-dependent protein kinase by tumor-promoting phorbol ester. J.Biol.Chem., 257:7847-7851.
22.Kraft, A. S. and Anderson, W. B.(1983).Phorbol ester increase the amount of Ca2+, phospholipid-dependent proteinase kinase associated with plasma membrane. Nature., 301:621-623.
23.Berridge, M. J. (1987) Inositol trisphosphate and diacylglycerol:
two interaction second messengers. Annu. Rev. Biochem., 56:159-193.
24.Rozengurt, E.(1989)Signal transduction pathway in mitogensis. Brit. Med. Bull., 45:515-528.
25.Housey, G. M., Johnson, M. D., Hsiao, M. L., O, Brain, C.A., Murrhy, J. P., Kirschmerier, P. and Weistein, I. B.(1988)Overproduction of protein kinase C cause disordered growth control in rat fibroblasts. Cell.,52:343-354.
26.Johnnes, F.-J., Prestle, J., Eis, S., Oberhagemann, P.and Pfizenmarier, K. (1994) PKCθis a novel atypical member of the protein kinase C family. J. Biol. Chem., 269:6140-6148.
27.Hug, H. and sarre, T. F.(1993)Protein kinase C isoenzymes:divergence in signal transduction. Biochemistry., 291:329-343.
28.Jaken, S. (1996)Protein kinase C isoenzyme and substrates. Curr.Opin.Cell Biol., 8:168-173.
29.Johnnes, F.-J., Prestle, J., Eis, S., Oberhagemann, P.and Pfizenmarier, K. (1994) PKCθis a novel atypical member of the protein kinase C family. J. Biol. Chem., 269:6140-6148.
30.Stabel, S(1994)Protein kinase C-an enzyme and its relatives. Semin. Cancer Biol., 5:277-284.
31.Azzi, A., Bosoboinik, D. and Hensey, C.(1992)The protein kinase C and protein kinase C related gene families. Eur. J. Biochem., 208:547-557.
32.Huang, K. P., Huang, F. L., Nakabayashi, H. and Yoshida, Y. (1988) Biochemical characterization of rat brain protein kinase C isoenzyme. J. Biol. Chem., 263:14839-14845.
33.Hocever, B. A., and Fields, A. P. (1991) Selective translocation of beta II-protein kinase C to the nucleus of human promyelocytic (HL60) leukemia cells. J. Biol. Chem., 266:28-33.
34.Hidaka, H., Tanaka, T., Onoda, K., Hagiwara, M., Watanable, W., Ohta, H., Ito, Y., Tsurudomw, M. and Yoshida, T. (1988) Cell type-specific expression of protein kinase C isoenzyme in the rabbit cerebellum. J. Biol. Chem., 263:4523-4526.
35.Liyanage, M., Frith, D., Liveneh, E. and Stable, S.(1992)Protein kinase C group B members PKC-delta, -epsilon, -zeta and PKC-eta. Biochem. J., 283:781-787.
36.Sasaki, Y., Hayashi, N., Ito, T., Fusamoto, H., Dato, N. and Kamada, T.(1989)Hetergeneous activation of Protein kinase C during rat liver regeneration induced by carbon tetrachloride administration. FEBS Lett., 254:59-65.
37.Alessenko, A., Khan, W. A., Westel, W. and Hannun, Y. A.(1992)Selective changes in Protein kinase C isoenzymes in rat liver nuclei during liver regeneration. Biochem. Biophys. Res. Commun., 182:1333-1339.
38.Okamoto, A., Nishimura, K., Nakagawa, M. and Nakano, H.(1988)Protein Kinase C in the regeneration rat liver. Biochemistry., 207:304-310.
39.Rush, J. S., Klen, J., Fanti, P., Bhat, N. R. and Waechter, C.J.(1992)Direct assay of membrane-associated protein kinase C activity in B lymphocyte in the present of Brij 58. Anal Biochem., 207:304-310.
40.Dong, L., Stevents, J. L., Fabbro, D. and Jaken, S.(1993)Regulation of Protein kinase C isoenzymes in kidney regeneration. Cancer Res., 53:4542-4549.
41.Kondo, T., Inui, H., Konishi, F. and Inagami, T.(1992)Phosphatease D mimics plateate-derived growth factor as a competence factor in vascular smooth muscle cells. J. Biol. Chem., 267:23609-23616.
42.Carpenter, G.(1992)Receptor tyrosine kinase substrate:src homology domain and signal transduction. FASEB J., 6:3283-3289.
43.Cowley, B. D., Chadwick, L. J, Grantham, J.J. and Calvet, J. P.(1989)Sequence protooncogene expression in regeneration kidney following acute renalinjury. J Boil Chem., 264:8389-8393.
44.張愛琪(1997)碩士論文,中山醫學院生化所
45.劉惠珍(1999)碩士論文,中山醫學院生化所
46.Kubota T, Kamada S, Taguchi M, Sakamoto S and Aso T.(1993)Protein kinase C activation and calcium mobilization decrease prolactin release from human decidual cells in early pregnancy. J Endocrinol., 137(2):335-40
47.Ogle TF, Dai D and George P.(1999)Progesterone-regulated determinants of stromal cell survival and death in uterine decidua are linked to protein kinase C activity. Steroids., 64(9):628-33
48.Kekule, AS., Lauer, U., Meyer, M., Caselmann, WH., Hofschneider, PH., and Koshy, R.(1990) The PreS2/S region of integrated hepatis B virus DNA encodes a transcriptional tranactivator. Nature., 343(6257):457-461
49.Luber, B., Lauer, U., Weiss, L., hohne, M., Hofschneider, PH. and Kekule AS.(1993)The hepatitis B virus transactivator HBx cause elevation of diacylglycerol and activation of protein kinase C. Res Virol 144(4):311-321
50.Kekule, AS., Lauer, U., Weiss, L., Luber, B. and Hofschneider, PH.(1993)Hepatitis B virus transactivator HBx uses a tumor promoter signaling pathway. Nature 361(6414):742-745
51.Rodgers, W. H., Osteen, K. G., Matrisian, L M., Navre, M., Giudice, L. C., Gorstein, F. G.(1993)Expression and localization of matrilysin, a matrix metalloproteinase, in human endometrium during the reproductive cycle . J. Obstet. Gynecol., 168:9413-9417
52.王意維(2000)碩士論文,中山醫學院生化所
53.Heldin, C. H. and Westermark, B.(1998)Role of tissue inhibitor of metalloproteinase-2 in regulation of pro-gelatinase A activation catalyzed by membrane-type-1 matrix metallopproteinase in human cancer cell. J. Biochem., 124:462-470
54.Signe F. Bjorn, Nina Hastrup, Leif R. Lund, Keld Dano, Jorgen F. Larsen. and Charies Pyke.(1997)Coordinated expression of MMP-2 and its putative, MT1-MMP, in human placentation. Molecular Human Reproduction., 3:713-723.
55.Van Dijk, M., Muriana, F.J., van Der, Hoeven, P.C., de Widt, J., Schaap, D., Moolenaar, W.H. and van Blitterswijk, W.J.(1997)Diacylglycerol generated by exogenous phospholipase C activates the mitogen-activated protein kinase pathway independent of Ras- and phorbol ester-sensitive protein kinase C: dependence on protein kinase C-zeta. Biochem. J., 323:693-699
56.Berra, E., Diaz-Meco, M.T., Dominguez, I., Municio, M.M., Sanz, L., Lozano, J., Chapkin, R.S., and Moscat, J.(1993)Protein Kinase C zeta isoform is critical for mitogenic signal transduction. Cell., 74:555-563
57.Mischak, H., Goodnight, J.A., Kolch, W., Martiny-Baron, G., Schaechtle, C., Kazanietz, M.G., Blumberg, P.M., Pierce, J.H., and Mushinski, J.F.(1993)Overexpression of protein kinase C-delta and -epsilon in NIH 3T3 cells induces opposite effects on growth, morphology, anchorage dependence, and tumorigenicity. J. Biol. Chem., 268:6090-6096
Part II.
1.李岩、王艷玲(1999)肝癌防治錦囊,一橋出版社,p25-33
2.潘淑芬編譯(2001)醫學生物化學,藝軒出版社,p345-346
3.譚建民、廖桂聲(1999)中西醫會診系列:肝炎,書泉出版社,p3-4
4.潘淑芬編譯(2001)醫學生物化學,藝軒出版社,p347-352
5.胡亦瑜編譯(2001)肝病全書----A、B、C、D十大肝炎及肝癌,原水文化,p86
6.高尚德(2000)急慢性肝炎,行政院衛生署中醫藥委員會,p7-8
7.胡亦瑜編譯(2001)肝病全書----A、B、C、D十大肝炎及肝癌,原水文化,p87
8.高尚德(2000)急慢性肝炎,行政院衛生署中醫藥委員會,p29-32
9.Albanis E, Friedman SL.(2001) Hepatic fibrosis. Pathogenesis and principles of therapy. Clin Liver Dis. 5(2):315-34, v-vi. Review.
10.Li D, Friedman SL.(1999) Liver fibrogenesis and the role of hepatic stellate cells: new insights and prospects for therapy. J Gastroenterol Hepatol., 14(7):618-33. Review
11.Albert G.(2001) History, Heterogeneity, Developmental Biology, and Functions of Quiescent Hepatic Stellate Cells. Seminars in Liver Disease., 21(3):311-335
12.Blouin A, Bolender RP, Weibel ER.(1977) Distribution of organelles and membranes between hepatocytes and nonhepatocytes in the rat liver parenchyma. A stereological study. J Cell Biol., 72:441-455
13.Thimgan MS, Yee HF.(1999) Quantitation of rat hepatic stellate cell contraction: stellate cells' contribution to sinusoidal resistance. Am J Physiol., 277:G137-G143
14.Kolodney MS, Thimgan MS, Honda HM.(1995) Ca2+-independent myosin II phosphorylation and contraction in chicken embryo fibroblasts. J Physiol., 515(part 1):87-92.
15.Friedman SL.(1996) Hepatic stellate cells. Prog Liver Dis., 14:101-130.
16.Blomhoff R, Wake K.(1991) Perisinusoidal stellate cells of the liver: important roles in retinol metabolism and fibrosis. FASEB J., 5:271-277
17.Blaner WS, Hendriks HF, Brouwer A.(1985) Retinoids, retinoid-binding proteins, and retinyl palmitate hydrolase distributions in different types of rat liver cells. J Lipid Res., 26:1241-1251
18.Blomhoff R, Norum KR, Berg T.(1985) Hepatic uptake of [3H]retinol bound to the serum retinol binding protein involves both parenchymal and perisinusoidal stellate cells. J Biol Chem., 260:13571-13575
19.Blomhoff R, Rasmussen M, Nilsson A.(1985) Hepatic retinol metabolism. Distribution of retinoids, enzymes, and binding proteins in isolated rat liver cells. J Biol Chem., 260:13560-13565
20.Hendriks HF, Verhoofstad WA, Brouwer A.(1985) Perisinusoidal fat-storing cells are the main vitamin A storage sites in rat liver. Exp Cell Res., 160:138-149
21.Ramadori G, Knittel T, Schwogler S.(1991) Dexamethasone modulates alpha 2-macroglobulin and apolipoprotein E gene expression in cultured rat liver fat-storing (Ito) cells. Hepatology., 14:875-882
22.Friedman G, Liu LM, Friedman SL, Boyles JK.(1991) Apolipoprotein E is secreted by cultured lipocytes of the rat liver. J Lipid Res., 32:107-114
23.Reeves HL, Friedman SL.(2002) Activation of hepatic stellate cells--a key issue in liver fibrosis. Front Biosci., 7:d808-26. Review.
24.Friedman SL.(1999) Stellate cell activation in alcoholic fibrosis--an overview. Alcohol Clin Exp Res. 23(5):904-10. Review.
25.Bataller R, David A. (2001) Hepatic Stellate Cells as a Target for the Treatment of Liver Fibrosis. Seminars in Liver Disease., 21(3):437-451
26.Rockey D, Don C.(2001) Hepatic Blood Flow Regulation by Stellate Cells in Normal and Injured Liver. Seminars in Liver Disease., 21(3):337-349
27.Toda K, Kumagai N, Tsuchimoto K, Inagaki H, Suzuki T, Oishi T, Atsukawa K, Saito H, Morizane T, Hibi T, Ishii H.(2000) Induction of hepatic stellate cell proliferation by LPS-stimulated peripheral blood mononuclear cells from patients with liver cirrhosis. J Gastroenterol., 35(3):214-20
28.Friedman SL.(2000) Molecular Regulation of Hepatic Fibrosis, an Integrated Cellular Response to Tissue Injury. J Biol Chem., 275:2247-2250
29.Tsukamoto H.(1999) Cytokine regulation of hepatic stellate cells in liver fibrosis. Alcohol Clin Exp Res., 23(5):911-6
30.黃怡超(2000)台灣常用治肝病中藥方劑對肝硬化的實驗療效研究,行政院衛生署中醫藥委員會,89年度委託研究計畫摘要
31.Lin SC, Lin CC, Lin YH, Shyuu SJ.(1994) Hepatoprotective effects of Taiwan folk medicine: wedelia chinensis on three hepatotoxin-induced hepatotoxicity. Am J Chin Med., 22(2):155-68
32.Wasser S, Ho JM, Ang HK, Tan CE.(1998) Salvia miltiorrhiza reduces experimentally-induced hepatic fibrosis in rats. J Hepatol., 29(5):760-71
33.Aniya Y, Shimabukuro M, Shimoji M, Kohatsu M, Gyamfi MA, Miyagi C, Kunii D, Takayama F, Egashira T.(2000) Antioxidant and hepatoprotective actions of the medicinal herb Artemisia campestris from the Okinawa Islands. Biol Pharm Bull., 23(3):309-12
34.Lin SC, Chung TC, Lin CC, Ueng TH, Lin YH, Lin SY, Wang LY.(2000) Hepatoprotective effects of Arctium lappa on carbon tetrachloride- and acetaminophen-induced liver damage. Am J Chin Med., 28(2):163-73
35.Nan JX, Park EJ, Kim YC, Ko G, Sohn DH.(2002) Scutellaria baicalensis inhibits liver fibrosis induced by bile duct ligation or carbon tetrachloride in rats. J Pharm Pharmacol., 54(4):555-63
36.You H, Wang B, Wang T.(2000) Proliferation and apoptosis of hepatic stellate cells and effects of compound 861 on liver fibrosis. Zhonghua Gan Zang Bing Za Zhi., 8(2):78-80
37.Liu CH, Liu P, Hu YY, Xu LM, Tan Y, Wang ZN, Liu C.(2000) Effects of salvianolic acid-A on rat hepatic stellate cell proliferation and collagen production in culture. Acta Pharmacol Sin., 2000;21(8):721-6
38.Liu P, Hu Y, Liu C, Liu C, Zhu D.(2001) Effects of salvianolic acid-A (SA-A) on liver injury: SA-A action on hepatic peroxidation. Liver., 21(6):384-390
39.呂通信(1996)台灣民間常用青草藥總整理(下),青原藥用植物研究中心,p200
40.王伯祥(2000)中醫肝膽病防治大全,正中書局,p89
41.王伯祥(2000)中醫肝膽病防治大全,正中書局,p108
42.許鴻源(1972)台灣地區出產中藥材圖鑑,行政院衛生署中醫藥委員會,p294
43.甘偉松(1981)藥用植物學,國立中醫藥研究所,p485
44.李岡榮(2000)即將失傳的台灣青草藥(下),p260
45.Wei ZM.(1996) Effects of injection Salviae miltiorrhizae on senile chronic asthmatic bronchitis patients. Zhongguo Zhong Xi Yi Jie He Za Zhi. 16(7):402-4. Chinese.
46.Miski M, Ulubelen A, Johansson C, Mabry TJ.(1983) Antibacterial activity studies of flavonoids from Salvia palaestina. J Nat Prod. 46(6):874-5
47.林榮貴、張建雄、林俊清(1993)嘉南學報,vol.19,p21-32
48.林俊清、蔣連財(2000)中華民國生藥學會學術研討會
49.方博正.(2001)碩士論文. 台灣產生藥之抗氧化及抗皰疹病毒活性資源開發研究.高雄醫學大學天然藥物研究所.
50.黃曉元.(1997)碩士論文. 九層塔與七層塔精油對大白鼠初代肝細胞中GSH相關之抗氧化與解毒代謝系統之影響.中興大學食品科學所.
51.Ferrandiz ML, Bustos G, Paya M, Gunasegaran R, Alcaraz MJ.(1994) Hispidulin protection against hepatotoxicity induced by bromobenzene in mice. Life Sci. 55(8):PL145-50.

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