第一部分.
我們過去的研究中發現，在蛻膜形成（decidualization）的過程中，不論是在真懷孕或假懷孕大鼠中，蛋白激酵素C（PKC）異構體都參與其蛻膜的形成，因此我們猜測PKC可能參與調節蛻膜細胞（decidual cell）的增殖過程。本實驗藉由RT-PCR的技術來分析假孕大鼠蛻膜形成過程中，PKC異構物及其下游相關蛋白以及細胞增殖指標（cdc2，cyclin D1）之mRNA表現，再輔以DNA定序分析確定RT-PCR產物之專一性。結果發現PKCα mRNA表現量在假孕大鼠蛻膜形成過程第二天到第九天中有顯著的增加，而同時期中PKC相關轉錄因子c-jun，c-fos亦有明顯的增加，這些明顯的改變意味著在蛻膜組織中PKCα的活化。此外也發現在假孕大鼠蛻膜形成過程中PKCδ及PKCλ mRNA表現量增加，而PKCλ mRNA表現增加的同時細胞增殖指標（cdc2及cyclin D1）mRNA的表現量亦增加。因此由以上的實驗結果證實了PKC異構物參與子宮蛻膜的形成過程。
第二部分.
肝炎（Hepatitis）在台灣地區素有國病之稱。根據流行病調查發現肝炎與肝纖維化和肝硬化有相關性。肝纖維化是一個動態的過程，在初期是為可逆的階段。因此，目前許多研究室仍致力於尋找阻延肝纖維化之藥物，其中包含中草藥。另外，根據研究指出肝星狀細胞（hepatic stellate cells，HSCs）過度活化增殖是導致肝纖維化/肝硬化的主要細胞之一。本實驗旨在建立一「快速細胞篩選模式」來篩檢中醫理論中具抗肝纖維化中草藥之功效。實驗選用36種中草藥，以傳統水萃取法萃取後，利用活化與未活化態肝星狀細胞偵測其半數致死劑量（LD50）。結果顯示上述36種中草藥中，確有抗纖維化功效者為31種（86.1 %），進一步分析後發現其中22種（61.1 %）中草藥其對於未活化態肝星狀細胞較不具傷害性，顯示利用肝星狀細胞篩選模式對於抗肝纖維化中草藥之篩選可能較優於傳統之動物模式。

Part I.
Our previous study have demonstrated that the protein levels of PKC isoforms were altered in the proliferation stages of decidualization at pseudopregnant and pregnant rats. In this study, we measured the mRNA expressions of the PKC isoforms and their down-stream related genes by using RT-PCR. The product of RT-PCR was also checked by DNA sequence analysis. The results showed that the levels of PKCα mRNA was significantly increased from day 2 to day 9 in the decidualization. The mRNA expression of the transcription factors (c-jun and c-fos) were also significantly increased during the same times. These specified that the alterations in the PKCα may signify their activation in decidual tissues. In addition, the mRNA levels of the other PKC isoforms (PKCλ and PKCδ were also significantly increased during the decidualization. The increase in the PKCλ mRNA was paralleled with the increase in the mRNA levels of the proliferation markers (cdc2 and cyclin D1). Thus, These data confirmed that the expression of PKC isoforms may be involved in the development of the decidualization.
Part II.
Hepatitis is one of the most common diseases in Taiwan. Patients with hepatitis may lead to liver fibrosis and cirrhosis. Liver fibrogenesis, which is a dynamic, complex, and progressive process, may be reversible at initial stages. Recently, many labs are extremely focus on discovering new drugs from Chinese herb for anti-fibrosis of liver. According to previous research, to activate hepatic stellate cells have been clearly identified as the major cause of liver fibrogenesis. The present studies aim to set up a fast selective cell model to estimate the anti-fibrotic effects by Chinese herbs. LD50 was determined by the actived/inactived HSCs when treated with water extracts of 36 kinds of Chinese herb. Our results showed that totally 31 kinds of Chinese herbs (86.1%) were detected with the antifibrotic effects. Within these 31 herbs, there are 22 kinds (61.1%) have less damage on inactived HSCs compared with actived. Taken together, our results indicated that the HSCs model may be more suitable to estimate the anti-fibrotic effects of Chinese herbs than animal models.

第一部分：假孕大鼠子宮蛻膜形成過程中蛋白激酵素C異構體mRNA之表現
壹、中文摘要
貳、英文摘要
參、縮寫表
肆、前言
伍、實驗動機
陸、實驗方法與材料
柒、實驗結果
捌、討論
玖、參考文獻
拾、圖表
附錄
第二部分：中草藥萃取物抑制肝臟發炎功效之評估
壹、中文摘要
貳、英文摘要
參、縮寫表
肆、前言
伍、實驗動機
陸、實驗方法與材料
柒、實驗結果
捌、討論
玖、參考文獻
拾、圖表
附錄

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