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研究生:傅慧雯
研究生(外文):Hui Wen Fu
論文名稱:應用ISSR研究玉山圓柏之遺傳變異
論文名稱(外文):Genetic Variation of Juniperus squamata var. morrisonicola Based on ISSR Markers
指導教授:蕭如英蕭如英引用關係
學位類別:碩士
校院名稱:國立中興大學
系所名稱:植物學系
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:82
中文關鍵詞:玉山圓柏遺傳變異ISSR
外文關鍵詞:Juniperus squamata var. morrisonicolagenetic variationISSR
相關次數:
  • 被引用被引用:7
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玉山圓柏(Juniperus squamata var. morrisonicola)生長在海拔3,000公尺以上高山,是台灣針葉樹種分布最高者,群落的形態有極大差異,生長型的變異範圍由匍伏灌木至直立喬木。以13個ISSR引子研究玉山圓柏10個族群160個個體,共得到173個條帶,其中83為多型性條帶(48.98%)。AMOVA分析顯示,地理區域間變方成分佔3.85%(P=0.0933),地區內族群間佔9.06%(P < 0.001),族群內個體間變方成分佔87.09%(P <0.001)。POPGENE的分析結果顯示,各族群內之基因歧異度值由0.294- 0.372,總的Nei’s基因歧異度(Ht)為0.384,遺傳分化係數(Gst)為0.117,基因流(Nm)為3.765。由這些結果看來,玉山圓柏族群基因流暢通,族群間分化程度與一般針葉樹相近。比較關山族群喬木與灌木兩種不同生長型個體,發現兩種生長型間無顯著遺傳差異。南湖大山地區的南湖池直立喬木族群與南湖大山北峰匍伏灌木族群間,有顯著的遺傳分化。
Juniperus squamata var. morrisonicola is distributed in mountains above 3,000m in altitude and is the conifer species with highest altitudinal distribution in Taiwan. The species is highly variable in growth forms, ranging from low procumbent to tall tree. One hundred and sixty individuals from ten populations were studied for ISSR variation. One hundred and seventy-three bands were observed using 13 primers. Eighty-three (48.98%) were polymorphic bands. AMOVA analysis revealed that the percentages of variance components attributable to variation among regions, among populations within regions, and among individuals within populations were 3.85% (p=0.0933), 9.06% (p<0.001), and 87.09% (p<0.001), respectively. The result indicated that there was a slight but signification differentiation among populations. However, the major variation existed among individuals within populations. POPGENE analysis revealed that the total gene diversity (Ht) of the species was 0.384 and the gene diversities of populations ranged from 0.294 to 0.372. The population genetic diversity index (Gst) was 0.117 while the gene flow index (Nm) was 3.765. The result indicated that the gene flow among populations was relatively unrestricted. Population differentiation was similar to other conifer species. No genetic differentiation between shrub and tree growth forms was observed in Kuanshan population. However, a significat genetic differentiation existed betweet tree-form Nanhuchi population and shrub-form Nanhutashanbefeng population in Nanhutashan region.
中文摘要----------------------------------------------Ⅰ
英文摘要----------------------------------------------Ⅱ
壹、前言---------------------------------------------- 1
貳、前人研究------------------------------------------ 3
參、材料與方法----------------------------------------12
肆、結果--------------------------------------------- 22
伍、討論--------------------------------------------- 35
陸、結論--------------------------------------------- 46
柒、引用文獻----------------------------------------- 48
王介鼎 1995 台灣穗花杉族群同位酵素變異之研究 國立台灣大學森林學研究所碩士論文
李鎮宇、姜家華、林讚標、王亞男 1995 紅檜五個族群同位酵素變異之研究 中華林學季刊28(1):3-20
林讚標、孫于卿、羅漢強、鄭育斌 2000 同功脢分析顯示台灣固有種台東蘇鐵之低遺傳歧異度 台灣林業科學15(1):13-19
林惠文 1997 以逢機多型性DNA分子標記探討紅檜族群之遺傳變異 私立中國文化大學生物科技研究所碩士論文
柳榗 1971台灣植物群落之分類Ⅱ、高山寒原及針葉樹林群系 林試所報告203:1-24
柳榗 1961 本省最新發現之一種寒帶林-香柏林 林試所通訊109:859-862
郭怡秀 1997 以逢機多型性DNA分子標記探討台灣扁柏族群之遺傳變異 私立中國文化大學生物科技研究所碩士論文
張國楨,姜家華,王亞男 1996 台灣櫸逢機擴大多型性核酸分析之研究 台大實驗林研究報告10(3):29-40
黃士元 2002 特有植物台灣粗榧保育資源研究-以族群遺傳變異及生態生理特性觀點 國立中興大學植物學系博士論文
曾于芯 2000 以RAPD研究鄧氏胡頹子之遺傳變異 國立中興大學植物學系碩士論文
黃朝慶,孫于卿 1995 清水圓柏分佈及生育地之調查研究 84年度試驗研究計畫執行成果(植物組) 台灣省特有生物研究保育中心印行
黃生 1991 基因流傳與遺傳分化 生物科學34:19-32
葉千萬、姜家華、王亞男 1996 利用RAPD研究台灣穗花杉族群之遺傳變異 台大農學院研究報告36(3):195-204
應紹舜1976 雪山地區高山植群的研究 中華林學季刊9(3):119-135
蘇鴻傑1974 台灣高山地區之香柏群落 台大實驗林研究報告113:101-110
蘇鴻傑1988 雪山香柏保護區植群生態之研究 林務局保育研究系列
陳玉峰1997 台灣植被誌第二卷 高山植被與高山植物(下) 晨星出版
Adams, R. P. and T. Demeke 1993. Systematic relationships in Juniperus based on random amplified polymorphic DNAs(RAPDs). Taxon 42 : 553-572.
Adams, R. P., L. E. Flounoy, R. A. Singh, H. Johnson, and M. Mayeux, 1998. Invasion of Juniperus ashei : A new theory based on random amplified polymorphic DNAs(RAPDs). Biochem. Syst. Ecol. 26 : 371-377.
Adams, R. P. 2000. Systematics of the one seeded Juniperus of the eastern hemisphere based on leaf essential oils and random amplified polymorphic DNAs. Biochem. Syst. Ecol. 28 : 529-543.
Casasoli, M., C. Mattioni, M. Cherubini, and F. Villani. 2001. A genetic linkage map of European chestnut (Castanea sativa Mill.) based on RAPD, ISSR, and isozyme markers. Theor. Appl. Genet. 102:1190-1199.
Cekic, C., N. H. Battey, and M. J. Wilkinson. 2001. The potential of ISSR-PCR primer-pair combinations for genetic linkage analysis using the seasonal flowering locus in Fragaria as a model. Theor. Appl. Genet. 103:540-546.
Excofier, L., P. E. Smouse, and J. M. Quattro. 1992. Analysis of molecular variance inferred from metric distance among DNA haplotypes: Application to human mitochondrial DNA restriction data. Genetics 131 : 479-491.
Fang, D. Q., M. L. Roose, R. R. Krueger, and C. T. Federici. 1997. Fingerprinting trifoliate orange germplasm accessions with isozymes, RFLPs, and inter-simple sequence repeat markers. Theor. Appl. Genet. 95:211-219.
Fang, D. Q. and M. L. Roose. 1997. Identification of closely related citrus cultivars with inter-simple sequence repeat markers. Theor. Appl. Genet. 95:408-417.
Fernandez, M. E., A. M. Figueiras, and C. Benito. 2002. The use of ISSR and RAPD markers for detecting DNA polymorphism, genotype identification and genetic diversity among barley cultivars with known origin. Theor. Appl. Genet. 104:845-851.
Hamrick, J. L. and M. J. W. Godt. 1989. Allozyme diversity in plant species. In A. H. D. Brown, M. T. Clegg, A. L. Kahler and B. S. Weri [eds.]. Plant population genetics, breeding and genetic resources: p.43-63. Sinauer. Sunderland, M. A..
Hollingsworth, M. L., P. M. Hollingsworth, G. I. Jenkins, J. P. Bailey, and C. Derris. 1998. The use of molecular markers to study patterns of genotypic diversity in some invasive alien Fallopia spp. (Polygonaceae). Mol. Ecol. 7:1681-1691.
Hess, J., J. W. Kadereit, and P. Vargas. 2000. The colonization history of Olea europaea L. in Macaronesia based on internal transcribed spacer 1 (ITS-1) sequences, randomly amplified polymorphic DNAs (RAPD), and intersimple sequence repeats (ISSR). Mol. Ecol. 9:857-868.
Hsiao, J. Y. and S. M. Lee. 1999. Genetic diversity and microgeographic differentiation of Yushan cane (Yushania niitakayamensis; Poaceae) in Taiwan. Mol. Ecol. 8 : 263-270.
Huang, J. C. and M. Sun. 2000. Genetic diversity and relationships of sweetpotato and its wild relatives in Ipomoea series Batatas (Convolvulaceae) as revealed by inter-simple sequence repeat (ISSR) and restriction analysis of chloroplast DNA. Theor. Appl. Genet. 100:1050-1060.
Hwang, S. Y., H. W. Lin, Y. S. Kuo, and T. P. Lin. 2001. RAPD variation in relation to population differentiation of Chamaecyparis formosensis and Chamaecyparis taiwanensis. Bot. Bull. Acad. Sin. 42 : 173-179.
Iruela, M., J. Rubio, J. I. Cubero, J. Gil, and T. Millan. 2002. Phylogenetic analysis in the genus Cicer and cultivated chickoea using RAPD and ISSR markers. Theor. Appl. Genet.104: 643-651
Joshi, S. P., V. S. Gupta, R. K. Aggarwal, P. K. Ranjekar, and D.S. Brar. 2000. Genetic diversity and phylogenetic relationship as revealed by inter simple sequence repeat (ISSR) polymorphism in the genus Oryza. Theor. Appl. Genet. 100:1311-1320.
Kojima, T., T. Nagaoka, K. Noda, and Y. Ogihara. 1998. Genetic linkage map of ISSR and RAPD markers in Einkorn wheat in relation to that of RFLP markers. Theor. Appl. Genet. 96:37-45.
Kobayashi, N., T. Horikoshi, H. Katsuyama, T. Hanhanda, and K. Takayanagi. 1998. A simple and efficient DNA extraction method for plants, especially woody plants. Plant tissue cluture biotechnology 4(2) : 76-80.
Lai, J. A., W. C. Yang, and J. Y. Hsiao. 2001. An assessment of genetic relationships in cultivated tea clones and native wild tea in Taiwan using RAPD and ISSR markers. Bot. Bull. Acad. Sin. 42:93-100.
Levi, A. and L. J. Rowland. 1997. Identifying blueberry cultivars and evaluating their genetic relationships using randomly amplified polymorphic DNA (RAPD) and simple sequence repeat- (SSR-) anchored primers. J. Amer. Soc. Hort. Sci. 122:74-78.
Lin, T. P., T. Y. Lee, L. F. Yang, Y. L. Chung, and J. C. Yang. 1994. Comparison of the allozyme diversity in several populations of Chamaecyparis formosensis and Chamaecyparis taiwanensis. Can. J. For. Res. 24:2128-2134.
Lin, T. P., C. S. Lu, and Y. L. Yang. 1993. Allozyme variation in four populations of Taiwania cryptomerioides in Taiwan. Silvae Genetica 42:278-284.
Martin, J. P. and M. D. Sanchez-Yelamo. 2000. Genetic relationships among species of the genus Diplotaxis (Brassicaceae) using inter-simple sequence repeat markers. Theor. Appl. Genet. 101:1234-1241.
Mattioni, C., M. Casasoli, M. Gonzalez, and R. Ipinza. 2002. Comparison of ISSR and RAPD markers to characterize three Chilean Nothofagus species. Theor. Appl. Genet. 104: 1064-1070.
McGregor, C. E., C. A. Lambert, M. M. Greyling, and L. Warnich. 2000. A comparative assessment of DNA fingerprinting techiques (RAPD, ISSR, AFLP, and SSR) in tetraploid potato (Solanum tuberosum L.) germplasm. Euphytica:Netherlands Journal of Plant Breeding 113(3) : 135-144.
Miller, C. I. and K. A. Marshall. 1991. Allozyme variation of Port-Orford-Cedar(Chamaecyparis lawsoniana):Implications for genetic conservation. For. Sic. 37:1060-2134.
Nei, M. 1973. Analysis of gene diversity in subdivided population. Proc. Natl. Acad. Sci. USA. 70 : 3321-3323.
Prevost, A. and M. J. Wilkinson. 1999. A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theor. Appl. Genet. 98(1) : 107-112.
Qian, W., S. Ge, and D-Y. Hong. 2001. Genetic variation within and among populations of a wild rice. Oryza granulata from China deteted by RAPD and ISSR markers. Theor. Appl. Genet. 102 : 440-449.
Ratnaparkhe, M. B., D. K. Santra, A. Tullu, and F. J. Muehlbauer. 1998. Inheritance of inter-simple-sequence-repeat polymorphisms and linkage with a fusarium wilt resistance gene in chickpea. Theor. Appl. Genet. 96:348-353.
Rohlf, F. J. 1993. Numerical Taxonomy and Multivariate Analysis System. Applied Biostatistics Inc., New York.
Sankar, A. A. and G. A. Moore. 2001. Evaluation of inter-simple sequence repeat analysis for mapping in Citrus and extension of the genetic linkage map. Theor. Appl. Genet. 102:206-214.
Slakin, M. and N. M. Barton. 1989. A comparison of three indirect methods for estimating average levels of gene flow. Evolution 43 : 1349-1368.
Sokal, R. R. and N. L. Oden. 1978. Spatial autocorrelation in biology. Some biological implications and four applications of evolutionary and ecological interest. Biological journal of the Linnean Society 10: 229-249.
Tomaru, N., Y. Tsumura, and K. Ohba. 1994. Genetic variation and population differentiation in natural populations of Cryptomeria japonica. Plant Species Biol. 9:191-199.
Uchida, K., Y. Tsumura, and K. Ohba. 1991. Inheritance of isozyme variants in leaf tissues of hinoki, Chamaecyparis Obtusa, and allozyme diversity of two natural forests. Japan J. Breed. 41:11-24
Weising, K., H. Nybom, K. Wolff, and W. Meyer. 1995. DNA Fingerprinting in plants and fungi. CRC press, Boca Ration.
Wolfe, A. D., Q. Y. Xiang, and S. R. Kephart. 1998. Assessing hybridization in natural populations of Penstemon (Scrophurariaceae) using hypervariable intersimple sequence repeat (ISSR) bands. Mol. Ecol. 7:1107-1125.
Wolff, K., E. Zietkiewicz, and H. Hofstra. 1995. Identification of chrysanthemum cultivars and stability of DNA fingerprinting patterns. Theor. Appl. Genet. 91:439-447.
Wolff, K. and M. M. Richards. 1998. PCR markers distinguish Plantago major subspecies. Theor. Appl. Genet. 96:282-286.
Wright, S. 1931. Evolution in Mendelian populations. Genetics 16 : 97-159.
Zietkiewicz, E., A. Rafalski, and D. Labuda. 1994. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplication. Genome 20 : 176-183.
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